LOCUS       CP128701               10060 bp    DNA     circular BCT 03-JUL-2023
DEFINITION  Klebsiella pneumoniae strain KPN23 plasmid pKPN23_2, complete
            sequence.
ACCESSION   CP128701
VERSION     CP128701.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN35878164
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Kochan,T.
  TITLE     Klebsiella pneumoniae Clinical Isolates with Features of Both
            Multidrug-Resistance and Hypervirulence Have Unexpectedly Low
            Virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Kochan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2023) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. v0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 174.927069977949x
            Sequencing Technology :: Oxford Nanopore MinION/GridION, Illumina
            NovaSeq/MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/26/2023 13:52:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,405
            CDSs (total)                      :: 5,282
            Genes (coding)                    :: 5,141
            CDSs (with protein)               :: 5,141
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 72 of 141
            Pseudo Genes (incomplete)         :: 74 of 141
            Pseudo Genes (internal stop)      :: 32 of 141
            Pseudo Genes (multiple problems)  :: 29 of 141
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN23"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN23_2"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.8945 N 87.6208 W"
                     /collected_by="Fiorella Krapp"
     gene            complement(729..878)
                     /locus_tag="QU722_26965"
     CDS             complement(729..878)
                     /locus_tag="QU722_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WJU25784.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(963..1220)
                     /locus_tag="QU722_26970"
     CDS             complement(963..1220)
                     /locus_tag="QU722_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015643 - toxic substance binding [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WJU25785.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(1230..2915)
                     /locus_tag="QU722_26975"
     CDS             complement(1230..2915)
                     /locus_tag="QU722_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WJU25786.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3242..3487
                     /locus_tag="QU722_26980"
     CDS             3242..3487
                     /locus_tag="QU722_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJU25787.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3761..4132
                     /gene="tolA"
                     /locus_tag="QU722_26985"
     CDS             3761..4132
                     /gene="tolA"
                     /locus_tag="QU722_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0098567 - periplasmic side of plasma membrane [Evidence
                     IEA]; GO_function: GO:0005215 - transporter activity
                     [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WJU25788.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            4129..4494
                     /locus_tag="QU722_26990"
     CDS             4129..4494
                     /locus_tag="QU722_26990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015291 - secondary active transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WJU25789.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            4669..4947
                     /locus_tag="QU722_26995"
     CDS             4669..4947
                     /locus_tag="QU722_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WJU25790.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4935..5249
                     /locus_tag="QU722_27000"
     CDS             4935..5249
                     /locus_tag="QU722_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0009432 - SOS response
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WJU25791.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5413..5841
                     /locus_tag="QU722_27005"
     CDS             5413..5841
                     /locus_tag="QU722_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJU25779.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            5867..6046
                     /locus_tag="QU722_27010"
     CDS             5867..6046
                     /locus_tag="QU722_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WJU25780.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6073..6603)
                     /locus_tag="QU722_27015"
     CDS             complement(6073..6603)
                     /locus_tag="QU722_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJU25781.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6610..7341)
                     /gene="mobC"
                     /locus_tag="QU722_27020"
     CDS             complement(6610..7341)
                     /gene="mobC"
                     /locus_tag="QU722_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WJU25782.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(7341..9305)
                     /locus_tag="QU722_27025"
     CDS             complement(7341..9305)
                     /locus_tag="QU722_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WJU25783.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7915
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          48..626
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6614..7341)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 atacaaagtt acccacagat tccgtggata agcaggggac taacatgtga ggcaaaacgg
       61 cagggccgcg ccggtggcgt ttttccatag gctccgccct cctgccagag ttcacataaa
      121 cagacgcttt ttcggtgcat ctgtgggagc cgtgaggctc aaccatgaat ctgacagtac
      181 gggcgaaacc cgacaggact taaagatccc caccgtttcc ggtaggtcgc tccctcttgc
      241 gctctcctgt tccgaccctg ccgtttaccg gatacctgtc cgccgttctc ccttgcggga
      301 agtgtggcgc tttctcatag ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct
      361 ccaagctggg ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc ttatccggta
      421 actgttcact tgagtccaac ccgggaaagc acgcaaaacg ccactggcag cagccattgg
      481 taactgggag ttcgcagagg atttgtttag ctaaacacgc ggttgctctt gaagtgtgcg
      541 ccaaagtccg gctacactgg aaggacagat ttggttgctg tgctctgcga aagccagtta
      601 ccacggttaa gcagttcccc aactgactta accttcgatc aaaccacctc cccaggtggt
      661 tttttcgttt acagggcaaa agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      721 gatcttttct actgaaccgc gatccccgtc agttcagagg aggacgatgg tgccaccgtt
      781 ccgccctgaa catcccggat ataatttgcc tgacatgcgg ccagaagaaa gccagcaaca
      841 atcaatataa aaagaaaaat agtctttacc tttttcatat actttccctg tatttaaaga
      901 ctccggcatt catccctgaa caccggaagt cagtatttca aatcatcctt gataattttt
      961 tgttaccagt taccgtcccg ataatcaaaa gctatataac aattatccac atcaggtttt
     1021 atttgatttt taaaaaatgg ctgtagtaaa ggaacccatt ctttcttcaa atcaaactca
     1081 ccattattaa tattatcctt taaaggcatt cccaagcttt caatgacaga accatcgtca
     1141 ccaaagtctt tagaatactc ttccccttta aactcttcag ttttcttatc aaaccatgtc
     1201 aaacgtaatt taagccccat atttacctct caaagatatt ttttaatgtt ccgttttgga
     1261 tctggccctt taacctgctt acccgttttg ggatcaaatg cgcctaggtg ttcgccatca
     1321 ctggcacgat acccttcaag ctcaccgtgc tgggaatccc attcataaat tttacgtttt
     1381 ttatcgccat accatcgggc ccgcttacca ccaccacctt gtttaggtgt tttaggatca
     1441 cctcgcttta actctccaag tccctttatg tcttctgttt taggtactgg atgataatca
     1501 tggccgtagt ctttagtccc cttgcgaggc ttgttctttt cctcatttaa cttattttct
     1561 gcatccttcg cttttttctc cttttgtttc cggctctcca tcgccccggt aagtgcagcc
     1621 ttatctctgg ataaggattc attcattgcg tttaaagatg cctgagccgc ttttaatttc
     1681 tcattctgat tctgaatatc cttatcaaga tagcgaacct gatcctgcca cccggagcca
     1741 tattcatgag gcgggttata agtcacaaaa tcatctttat ttgcctctgc ctcttttacc
     1801 tttttatcgg cttcctgaac agcagccctt gcaccgggga gcgccttctc tgtcgatgca
     1861 atgcggctgt ttaaggtcgc aatattttta tcttcggcat ccagctcagc tttcgcttta
     1921 tcatagtctc ttttcagccc ctcttccgga tgagcggcgt cccatgcctg ctggcgacgc
     1981 ttttcttcct cctgacgctg ttttacctgt gccggggtaa gaacatctgt caccgacaca
     2041 taaaccggct tctgtccggt ctctttcggg aaacgaataa cggcctcacg ggagttacca
     2101 ccggctgtaa aaccagccgg acgtgaatca cctttttcag caacaatgcc ttttggcggg
     2161 gctttcgctg tcggaacacc tttcggtacg ctcacctgca gagacgggag acccggaatc
     2221 gacacactga atacccccgg acgttttgtc ggtttcgcat ccaccacagg gacactcatt
     2281 ggccgacctg cgacaaccgc aatatgctgc cgctcatcct tcaccacatc cacaacccgt
     2341 tgtctgacac gaaccgtcgc ctggtccagt ggtaaagtac ttgccggagt ctccgtcacc
     2401 gcatcggccg gtaatgacgt cataattttt gacatcattt tcggatcatc ttttgcaatc
     2461 tcagaaggca gcacaccgta gatcgcaatc ccccacagac caaacttaaa cggccccttc
     2521 agggcagcca gcacatcagc aacggcggcc gacaacgcat cgccggaaac tgataaagcc
     2581 agtccttcag cccccggagt agccagggca ggcagaccga aggccatcgc agtggccgaa
     2641 gactgtccgc caccagagct accgctatga ttgccgctgt taccaccatt accaccgggt
     2701 ttactcccgc cgttaccaaa ctcccccgga ttatagttat gaatatctcc attcggcgta
     2761 tgcgtcgtac cccagcccgg cccgctgttt ggatccgtac caccacctga tgaagggcca
     2821 cctttaccag acgtaccgtt cacattccca ctggcctgac caccattatg tcccagacct
     2881 gaattacccg gacctcgacc gtctccacca ctcataccgt tttcctcttt gacatttaaa
     2941 acaataaatt aaagccaaca tactgtacat atacacagta taattattac cttactcctc
     3001 ccgttttcag gtgtcaatat atgagattag ttctcatctt caggcacaaa aaagcgcccg
     3061 gagccacctg taagcagatc gcgtcttttc ccgaaaattt ctcatggcaa caaattttat
     3121 tatttaagac attcatcaca cttacatttc agatggtata gacacatctg aaagctaact
     3181 ggcatgagga gaggacctgc ggttaaactg ttcgggaaac ctgagacgcc cggaggccac
     3241 tatgacgcac ctgaaactga ctcccgctga agaaaagcgc ctggcggacg cctttgtcgc
     3301 tcaggcactg gccatgggcc tgaaagggaa caaaacgctg ccaaaggcgg taaaacaaca
     3361 ggtgcgggaa agggtgcttc gtgccattca ggacgaacgt gaagccaggc gggcgcagga
     3421 aatgcgtcgg gagaaatacg aggctacgga aaccaccttc acctggcagc cacagcgccg
     3481 ccggtaaggg gtatttacca taacgtcgca ttgcccgcac cgctcagacg gtctgagggc
     3541 gtttccggcg gctacgggat aaaacaggca acaaaccacc cggaaaaccg ccggaaacgc
     3601 gcccggtaaa ttgtaacccg ctgcatgcct atgcacgcaa ctgcatggcg ggccgtttgg
     3661 tgtaaaaacg atacgataag ccgtgtttat gtaaatgaac gtggccgtaa agagtgacaa
     3721 atatacatgt aacatttttt actgacaggt catcaacccg atgcgtttaa taccctttat
     3781 cctgctggca ttgtgcagcc ccttactcct gacggcctgt aagcattcca ccacccagcc
     3841 gccgaaaatt tatactctgg ataagtcatc taaaatcagt agctacgctg cggccatcaa
     3901 gaccgctgtt gaaatgcaga tgtttgaacc agaggcctat gcgggaaaaa tgtgcaccgt
     3961 gcggatatcc atacaacgtg atggcactct gaacagcgcc attgctgaag gaggagatcc
     4021 gaaactctgt aaagcagcaa tatcagccgt tacccacgcc aaaattccgc cggcaccgga
     4081 tgaaaatacc tggcaaatat tcaaaaacgc ccctctggat ttcagaccat gaaagctttt
     4141 actctgacag tgctggcggc catgttcctc actccggtac atgcgatacc actaaaaccc
     4201 gtaaccacac tgcagccaac ctacccggta caggccgcag caaaacgcat cgaaggtcag
     4261 gtgaccgcca cgtttgatat tctggacgat ggacgagtgg acaacgtgtc gcttaccggc
     4321 tgtcaccttt tttttcggga aacgctgacg gcaatgaggc actggctata tcccgcaggg
     4381 cacccggcaa tgcgacaggt tataaatttt gaattcagtc tgaaaaatga cggcttacag
     4441 gatccgcgac cagtcaccac ccaaaagctc aatgatgatg gttgccgcca gtaacaggtc
     4501 ctttaacccg caggctgcaa tagtcctgga tgatgagtga gcctgtagca gccctgatta
     4561 gtggaagcga agctgaaaaa tgggcagcaa aagccgtatt tctggcaaat agcgccgcca
     4621 cggattaaag tagtcatgtg taatacatat gactacaaag gggcttttat gtcatcagca
     4681 acatccatca ggctcgatga agaacttaag gaccggctga aaacgctggc cgatgaccgg
     4741 caccgctcag cgcacgcgct aatgctggag gcaatcaccg aatacatcga ccgggaagag
     4801 aagcgcagcc agtacctgcg ggacggacag gccgcatggc agcactatca ggaaacgggt
     4861 ctgcacctta ctgcggaaga agcggaagcc tggataagta cctggggaac tgagaacgag
     4921 caggatgctc cgccatgcca caggtaacga tttccgcgct ggcacagcgc gatttacaac
     4981 gccttcagga ctttctcaaa accaaaaatc ggctggcagc cagaaaagga ggtgaggtga
     5041 tcgtccgggc tatccagcaa ctgaaaacat tgccagacat tggccgcccg gtgccgtttc
     5101 tgccgctgga atataaggaa ctggtgatcg ggtttggcga cagtggctat gtgatgctct
     5161 accgccacga ccgggaaatg gaccagattg tgatcgtcac cgtcaggcat cagaaagaat
     5221 ccgggtatcc gggggcagac agcctctaat aaccgatgac gggggcgcaa ttcattgcgt
     5281 ggccgtcgcc ggtttgacaa gcgcagcgcg tcagaaaacc acatcctgaa cggaaaaacg
     5341 ggtaaaattc agcgatacat ttaccgtgag aaattacaca cctttcacac ccgataaaat
     5401 caggggtata cagtggcgaa aacattgctc aacctttcac aggcagcaca agccgcgggc
     5461 attaccagaa gaacgctata caatcatgta aaacagggaa aggttaccgt gtcacgtgac
     5521 gggaaaaaca accctgtcgt ggatgtgtca gaactgatcc gtgtatacgg aaatgtgaat
     5581 atccctgaga aacaaattcc cggcatttca caccgtgaaa atacacaaaa aaatttccca
     5641 caggaacagc ttctggcaat gcaaaaggaa ctggccgatc tccggcaggc tgtcaccctg
     5701 atgctggaag ataaaacatc ccgggaagaa gaacgccgcc agcacgatga tgagcgccgg
     5761 aaactccagg ccgaagtgga cagattgaca acagaactga cacagaagaa aaaacgcttc
     5821 tggtccggct ggtttagctg agaacttacc gaagggaatg gagaacatga cgaagcagga
     5881 aaaaactgcc ctcaatatgg ctaaattcat taaggatcag tcgctgctgt tgctggaaaa
     5941 gctgaacgag ctggacatgg ataccccggc cgacatgtgc gaacggcttc acgaggatgc
     6001 ggaaacactc tggcaggtga tgcgggataa gctcggggaa gaataacagc accggccatg
     6061 ccggtgctgg cgttattttt tgccgaacgg ccaccaccgg cgggcgggtt tgtcctccgg
     6121 cagttcaggc accggcacgt taaaatagcg gctgttatat tcccgcatca gctccgcttt
     6181 cagcttctcg ttcaccgcgc tttccccgtg ccgctcgctg accacctcaa tcatcgcgtt
     6241 aatctgctcc ggtgagcact tcccggcatc aaccacctgc acaccgttct cgatggcgaa
     6301 ccgctccacc tttcgataga ggtaatcaca cagcttcatt cctgtgggtt tgtcccgttc
     6361 ccattgcgga ttttcaccgg tgaaaaagta cgtggctttt tcgtcgctgt tgagccgctc
     6421 gtcatacatc ccgatggatt tcatcatcgc cagatgctgg gcccggtcga caaaacagaa
     6481 aacatacgcc ccggtctgcc aggcgagcac caccatgtct tccagatgct cgctggttgg
     6541 cataatgcag cgctcgccgt ctttgtcttc cacgtcctca ataaatacca tgtacagcgc
     6601 cataatccct cactccgttg tcataaattt aaagcacgcg aacagcctct cccggtccag
     6661 ctccaccgtt ttgcgggcct cgccggtctg tggattcacg atttcatcca ccgccagaat
     6721 cgcccgctgt acccggacaa gcgtcggccg gtcagggcag atgtaccaga tttcatcgat
     6781 gccgttcgcc cggcggttga acagataccc ggacaccact tcctgatacc gcctgcggct
     6841 cttgacggtg cgctctacct caaatgccac cgttctgccg tccagctccg ccagcgcgtc
     6901 cggcactttc atccctttca gggccatacg gtgcaggtac cgccagccgg tggccccccg
     6961 gccctccatg gagaggcgca gggactgcac ggccagccgg tgcggcagct gcgccgccga
     7021 cacccttgac ggctgaaacg gaataacgtc cgtcagcggc tcgtcctcgt cgaacgagaa
     7081 cgccaccccg tgcggcgtca gcccccacac gctgacctga cggccgtcaa ccgggtattt
     7141 cgcccggacc agcagccctt tcgccaccag actgtccagg aagcggtaaa ccggctgtac
     7201 cgccgcaatc cccagccgtc gcgcaaccag ctccgccgtg gaacacagct cttcccgcag
     7261 ccagcgcagg gtcagccgtg ttttctccgc gttccgcgcc tgccgcttcg cgtggctgac
     7321 gttgtactgc tccagtgtca tcagtaaagc cccgcaaaat catcatccgc ttttggtccg
     7381 ccgccgtcgg ccggaggcag gtactccgcc agcgggtccg ttttcgccag caccgggaag
     7441 gttttcagcg aaatttcccg gcgctcaacc cgcagcgggc tgctgaacgc cagccgcgcc
     7501 acgcccagcc cggtcatcac cgcgaacccg tcaggcaggt gttgcagggt gttcacgtca
     7561 aacagcggcc gcgcctcctt ctggatgaag ctgtcgccgc tgatgtgctc cacgttcccc
     7621 gcctcccggc tggcccggcg gcgctccacg tccacccgga tctccccggt ctggcccgcc
     7681 gcccactgcg cggactcctg gctggccgcg cgatagaacc agcggatcgg cgtgttatcc
     7741 agtaccgtgg ttttcacaaa atcagcgggc agatcctgcc cgcactgacc gaagtcgccc
     7801 agcgactggt gcgcgagcag caggttgcag tcccggctgc gcagcgtccc cagcgcgttc
     7861 agcacgtact tcgacagcag atatttaatc tcatcgagca tgatgctcgc gtgcggccac
     7921 ctccggaact catcccgcgc aatgatgatt tgcgcgcagc gggcaaacag catcttctgc
     7981 acccggatca ccgcctcatc gtccatcgag ccgatgacgt acaggcagcc gccgccgttg
     8041 attatccccg ccacatcgat accgctgtcc gtctgcagga cgctcaggtc ggccacgttc
     8101 tccagcttgg tgatcagccc cttcacgtcc tttttcaggg cctccggcaa cgcgtacgcc
     8161 gccgccagca cctgctgaat gtttgccccc tgcgggaact gctccgcaat cagcttcgcc
     8221 gctttctgct cgctgatgcg gtagtggtcc gccacgtcgc cggtctcact caggttaaac
     8281 ccggcaacca gcagattctt cagggcgtac tggtcgagat cgcggaacag attcagctgc
     8341 gccggtttcc ccggccgcag atccagcagg gtgaagtccg ggcagtgcgc ccggaagacg
     8401 tgcggcgccc acgcgtcccc tttggggtcg aagtacacca cagcgtcccc gaaatcgcgt
     8461 acgcagcgaa tgagcgcgtt ggtggccatc gcgctcttgc cgctcccggg caggccgagg
     8521 atctgaatat tggtcttgcg ccaggtatca cgagggacca gcaccggctg gccctgctca
     8581 tccttaccca gaaacaccgc ctgctgtttc cgggcctgct ccgtggaaaa atcggccagc
     8641 gcgttaaccg gtaccggctc cccgaaatgc aggtggcgga cgtctgtcat ttctccggtc
     8701 cgctggctgc tgcgccggct ctcctgattc agtttcgcgt tcaccgcctc gtcagcggcc
     8761 gcaaaatgac catcaagcag caggaacgcc agcagccgcg ccagaaggac gccggcgaca
     8821 agccctccgg cacaccaggc tatcccgaac aggttagccg gctgcagcag gtaataatct
     8881 gcgttcgcat agaggtccgg gcgcaggacg gtttctcccc agctctcgat aaacacggcg
     8941 acaaccagcc cccccagaag ggccagaccg gcagcgccgg tcagccagta cgcgtagttc
     9001 cagacatgtc gccgtgccct gaccatccgc ccgggtgaat ccagcgaggc cagcaccgaa
     9061 aatggcacag tccggttatt atcgcgctta tagcgatcgg catactcccg ccgcgcctta
     9121 tggctcagcc ccacaaaacg cattcggccg gcccagagtt cagccagacc gaaaaacagg
     9181 ccgataacca gaaaaaaacc ggccagactg taatactccc ggtacagcca gctgacccac
     9241 gggtaaatct gccgggccag ctccgggata tgccactgcc aggcaagcga atccgtatta
     9301 aacatgcaat ctctctcctt cagacgacgg gtttaccggc gtgatgcgca gcccccgctg
     9361 cacaagccgc ggacatgccc caaggcatgg cggcggcggt cacgtccgtg gtgattgcgt
     9421 tcagctttca ccgcggaggc cagcctcttt ccgccccatg cggaaaaggc gcagccccgg
     9481 aggaacaacg agcacgcccc agcgtgtgag aggaaaaggc ggcaccccag tgcggtcttt
     9541 tccggtgttc cggcccccgt cagagttcgc catagcgagc ggctgaccac ccgcaaaatc
     9601 aggagcagct ttgctgcttc gtcaggtgcg tccagcaccg tggatttttg tgggtggtca
     9661 ggcgtgagcc tgacgggggc cgggtgaatt tctggcgtgg ccggcgtcca gccggacaga
     9721 gccagaaaga ggggaaaaga ccgtgcgtcg gtggcgtcca gccagcgtaa gcaaggtctt
     9781 ttcccctgaa gtcagccacg ccccagcggg gatgacttgc atttctccgc aaggagaaaa
     9841 aagcaacaga gataggttaa cgtaaaatgt tcagcgaaaa accgacatgg ttcacctatc
     9901 ctgataattg atcgtcaggg aatagaaaga cttaatcagg ggacaatatc ccacatcagc
     9961 gcgtccggtg accgtcttgc aggtgccggg gaaattgcgc ctcctcgctc actcggtcgc
    10021 tacgctccgg gcgtgagact gcggcgggcg ctgcggacac
//
