LOCUS       CP128694                9730 bp    DNA     circular BCT 03-JUL-2023
DEFINITION  Klebsiella pneumoniae strain KPN482 plasmid pKPN482_4, complete
            sequence.
ACCESSION   CP128694
VERSION     CP128694.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN35878166
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Kochan,T.
  TITLE     Klebsiella pneumoniae Clinical Isolates with Features of Both
            Multidrug-Resistance and Hypervirulence Have Unexpectedly Low
            Virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Kochan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2023) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. v0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 45.4541809495107x
            Sequencing Technology :: Oxford Nanopore MinION/GridION, Illumina
            NovaSeq/MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/26/2023 13:42:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,620
            CDSs (total)                      :: 5,500
            Genes (coding)                    :: 5,321
            CDSs (with protein)               :: 5,321
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 77 of 179
            Pseudo Genes (incomplete)         :: 116 of 179
            Pseudo Genes (internal stop)      :: 29 of 179
            Pseudo Genes (multiple problems)  :: 35 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN482"
                     /isolation_source="respiratory"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKPN482_4"
                     /geo_loc_name="USA:Houston"
                     /lat_lon="29.710689 N 95.400586 W"
                     /collection_date="2012-10"
                     /collected_by="Houston Methodist Hospital"
     gene            1297..3258
                     /locus_tag="QU720_28025"
     CDS             1297..3258
                     /locus_tag="QU720_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WJU05033.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3258..3989
                     /gene="mobC"
                     /locus_tag="QU720_28030"
     CDS             3258..3989
                     /gene="mobC"
                     /locus_tag="QU720_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WJU05034.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            3996..4526
                     /locus_tag="QU720_28035"
     CDS             3996..4526
                     /locus_tag="QU720_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJU05035.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4554..4733)
                     /locus_tag="QU720_28040"
     CDS             complement(4554..4733)
                     /locus_tag="QU720_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WJU05036.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4802..5179
                     /locus_tag="QU720_28045"
     CDS             4802..5179
                     /locus_tag="QU720_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WJU05037.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(5241..5540)
                     /locus_tag="QU720_28050"
     CDS             complement(5241..5540)
                     /locus_tag="QU720_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WJU05029.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5530..5793)
                     /locus_tag="QU720_28055"
     CDS             complement(5530..5793)
                     /locus_tag="QU720_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WJU05030.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            6170..7255
                     /locus_tag="QU720_28060"
     CDS             6170..7255
                     /locus_tag="QU720_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WJU05031.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            7252..9441
                     /locus_tag="QU720_28065"
     CDS             7252..9441
                     /locus_tag="QU720_28065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_function:
                     GO:0015343 - siderophore-iron transmembrane transporter
                     activity [Evidence IEA]; GO_process: GO:0015891 -
                     siderophore transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WJU05032.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     Typing          complement(2..497)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.39"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3259..3986
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 gcgcacactt caagagcaac cgcgtgttta gctaaacaaa tcctctgcga actcccagtt
       61 accaatggct gctgccagtg gcgtttttgc gtgtctttcc gggttggact caagtgaaca
      121 gttaccggat aaggcgcagc agtcgggctg aacggggagt tcttgcttac agcccagctt
      181 ggagcgaacg acctacaccg agccgagata ccagtgtgtg agctatgaga aagcgccaca
      241 cttcccgtaa gggagaaagg cggaacaggt atccggtaaa cggcagggtc ggaacaggag
      301 agcgcaagag ggagcgaccc gccggaaacg gtggggatct ttaagtcctg tcgggtttcg
      361 cccgtactgt cagattcatg gttgagcctc acggctccca cagatgcacc gaaaaagcgt
      421 ctgtttatgt gaattctggc aggagggcga agcctatgga aaaacgccac cggcgcggcc
      481 ctgccgtttt gcctcacatg ttagtcccct gcttatccac ggaatctgtg ggtaactttg
      541 cgagagttag cagcgcccgc cgcagtctca cggccggagc gtagcgactg agtgagggag
      601 gaggcgcaat atcccccctc ccgcagacgg ccaccggacg cgcggatgtg acatattgca
      661 ccgtgttacc gcctttccct tccctgccga taaatcattc agataggtga agtctgtcgg
      721 ttttctgctg aatattttac gttaacctat ctctgttgct tttttctcct tgcggagaaa
      781 tgcaggtcat ccccgctggg gcgtggctga cttcaggagg aaatcccctg cttacgctgg
      841 ctggacgcca ccgacgcacg gtctttcccc ctctttctgg ctcagctcgg ctggacgccg
      901 gccacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc acaaaaaccc
      961 acggtgctga acgcacctga cgaagcagca aagctgctcc tgattttgcg ggtggtcagc
     1021 cgctcgctat ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc actggggtgc
     1081 cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc tccggggctg cgccttttcc
     1141 gcttggggcg gaaagaggct ggcctccgtg gtgaaagctg aatgcaatca ccacggacgt
     1201 gaccgccgcc gccatgcctc ggggcatgtc cgcggcttgt gcagcagggg ctgcacacca
     1261 cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt ttaatacgga ttcgcttgcc
     1321 tggcagtggc atatcccgga gctggcccgg cagatttacc cgtgggtcag ctggctgtac
     1381 agggagtatt acggtctggc cggggctttc ctggttatcg gcctgttttt cggtctggct
     1441 gaactctggg ccggacggat gcgttttgtg gggctgagtc ataaggagcg acgggagtat
     1501 gccgatcgct ataagcgcga taataaccgg actgcgccat tttcggtgct ggcttcgctg
     1561 gattcgcccg ggcggatggt cagggcacgg cggcatgtct ggaactacgc gtactggctg
     1621 accggcgctg ccggtctggc ccttctgggg gggctggttg tcgccgtgtt tatcgagagc
     1681 tggggagaaa ccgtcctgcg tccggacctc tacgcgaacg cagattatta cctgctgcag
     1741 ccggctaacc tgttcgggat tgtctggtgt gccggagggc ttgtcgccgg catccttctg
     1801 gcgcggctgc tggcatttct gctgcttgac ggtcattttg cggccgctga cgaggcggtg
     1861 aatgcgaaac tgaaccagga gagccggcgc agcagccagc ggaccggaga aatgaccgac
     1921 gtccgccacc tgcatttcgg ggagccggta ccggttaacg cgctggccga tttttccaca
     1981 gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg ccagccggtg
     2041 ctggtcccgc gtgatacctg gcgcaagacc aatattcaga tcctcggcct gcccgggagc
     2101 ggcaagagcg tgatgggcac caacgcgctc attcgctgcg tacgcgattt cggggacgcg
     2161 gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg ggcgcactgc
     2221 ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa tctgtttcgc
     2281 gatctcgacc agtacgccct gaagaatctg ctggttgccg ggtttaacct gagtgagacc
     2341 ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag cggcgaagct gattgcggag
     2401 cagttcccgc agggggcaaa cattcagcag gtgctggcgg cggcatacgc gctgccggag
     2461 gccctgaaaa aggacgtgaa ggggctgatc accaagctgg agaacgtggc cgacctgagc
     2521 gtcctgcaga cggacagcgg catcgacgtg gcggggataa tcaacggcgg cggctgcctg
     2581 tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg tgcagaagat gctgtttgcc
     2641 cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga ggtggccgca cgcgagcatc
     2701 atgctcgatg agattaaata cctgctgtcg aagtacgtgc tgaacgcgct ggggacgctg
     2761 cgcagtcggg actgcaacct gctgctcgcg caccagtcgc tgggcgactt cgggcagtgc
     2821 ggccaggatc tgcccgctga gtttgtgaaa accacggtgc tggataacac gccgatccgc
     2881 tggttctacc gggcggccag ccaggagtcc gcgcagtggg cggcgggcca gaccggggaa
     2941 atccgggtgg acgtggagcg ccgccgggcc agccgggagg cggggaacgt ggagcatatc
     3001 agcggggaca gcttcattca gaaggaggcg cggccgctgt ttgacgtgaa caccctgcag
     3061 cacctgcctg acgggtttgc ggtgatgacc gggctgggcg tggcccggat ggcgttcagc
     3121 agcccgctgc gggttgaacg ccgggaaatc ccgctgcagc gcttcccggt gctggcgaaa
     3181 acggacccgc tggcggagta ccagccggag gccgggcggc agcggccggg tgatgatgat
     3241 tttgcggggc tgtactgatg gcactggagc gatacaacgt cagccacgcg aagcggcagg
     3301 cgcggaacgc ggagaaaacc cggctgaccc tgcgctggct gcgggaagag ctgtgttcca
     3361 cggcggagct ggttgcgcga cggctgggga ttgcggcggt gcagccggtt taccgcttcc
     3421 tggacagcct ggtggcgaaa gggctgctgg tccgggcgaa atacccggtt gacggccgtc
     3481 aggtcagcgt gtggggactg acgccgcacg gggtggcgtt ctcgttcgac gaggacgaac
     3541 cgctgacgga cgttattccg tttcagccgt caagggtgtc ggcggcgcag ctgccgcacc
     3601 ggctggccgt gcagtccctg cgtctcgcca tggaggcccg gggggcctcc ggctggcggt
     3661 acctgcaccg tatggccctg aaggggatga aggtgccgga cgcgctggcg gagctggacg
     3721 gcagaacggt ggcgtttgag gtggagcgca ccgtgaagag ccgcaggcgc tatcaggagg
     3781 tggtgtccgg gtatctgttc aatcgcaaag cgaacggcat cgatgaaatc tggtacatct
     3841 gcccggaccg ggcgacgcag gtcagggtgc agcgggcgat cctgtcggtg gatgaaatcg
     3901 tgaatccaca gaccggcgag gcccgcaaaa cggcggagct ggaccgggag cggctgtttg
     3961 cgtgctttaa atttatgaca acggagtgag ggattatggc gctgtacatg gtatttattg
     4021 aagacgcgga ggaccgggac ggcgagcgct gcatcatgcc gaccagcgag catcttgaag
     4081 atatggtggt gctcgcctgg cagaccgggg cgtatgtttt ctgttttgtc gaccgggccc
     4141 agcatctggc gatgatgaaa tccatcggga tgtatgacga gcggctcaac agcgacgaaa
     4201 aagccacgta ctttttcacc ggtaaaaatc cgcaatggga acgggacaaa cccgcaggaa
     4261 tgaagctgtg tgattacctc tatcgcaagg tggagcggtt cgccatcgag aacggtgtgc
     4321 aggtggttga tgccgggaag tgctcaccgg agcagattag cgcgatgatt gaggtggtca
     4381 gcgagcggca cggggaaagc gcggtgaacg agaagctgaa agcggagctg atgcgggaat
     4441 ataacagccg ctattttaac gtgccggtgc ctgaactgcc ggaggacaaa cccgcccgcc
     4501 ggtggtggcc gttcggcaaa aaataaacgg cagcaccggc catgccggtg ctgttattcc
     4561 ttcccgagct tatcccgcat cacctgccag agtgtttccg catcctcgtg cagccgctcg
     4621 cacatgtcgg ccggggtatc catatccagc tcgttcagtt tctcgagtaa cagcagcgac
     4681 tggtccttaa taaatttagc catattgagg gcagtttttt cctgcttagt catgttttct
     4741 gtccttccgg gtgtggtgtg cttcctgtcg ggtatactgt ctcaaaaccg gaggtgagac
     4801 aatggcgtgg gtgacggtga gacaggcgac ggaactgacg gggaaagccc ggagttccct
     4861 gtatagggat atggcgaaag gccgcgtatc ataccgtact gaagctgatg gtggtcgggt
     4921 ggtggatacc agcgaactta tccgggtgta cggtgaatta agacagattg agactcacga
     4981 gcgggacggg ttgagactgt cggatgagac tgaaaaaatg gcctccgacg cgttgatcgc
     5041 ggagataaaa gcactcaggg aggaggtggc cggactgcgg caggaaatgc aggccatgag
     5101 actgctggaa cacaaaccag agacagcaga gacaccggcc ggaacatcgc gccgatggtg
     5161 gcagtgggga aaacgctgac gcgctgtgct tgtccaaccg gcgacggcca cgcaatgaat
     5221 tgcgccccca tcaccggtta tcagcgaacg gtatggggat tctggcgctg gccaaccacg
     5281 gccagaaccc ggactttcct ggtctggtca tcaacatcat agaggacatg ctgtcccatc
     5341 agactgatcc ggcgaacgtc ctcgccatag cgtgagtcca cttcatatag gcgcgggaag
     5401 tcggccagtt tgtccgctgc agcgagaact cgatcggtaa cgttctgcgc atcccacagg
     5461 gatgaatttt ccgcaatgta gtcaaacaat gctgtcacat cctcgatggc tccctgcttc
     5521 cattccacct catacggcat cgcgtagttc ctgatagcgg ccatgcagtc tctggcggag
     5581 ttgttccgtg gtcatgcccg ggcgcgtgtc agcgcgggcg gcggtcactt tagcgcgaac
     5641 ctgatcttca tattctgcct gggccagttc ttcgttagcc aggcgctgca acagtgcagc
     5701 tttgtccggg ccaccttcag gcagtatgcg gggcagtctc tcggccagtt cccgcaggaa
     5761 attggcgttt tcatagtgtg tactgttgct cattgtggag cctcccgggc tgatacgtcg
     5821 atatgaagga gtttagcagg gggcggtctg aaagaatatg ctcgtgtcag tcaggctgat
     5881 gaagacgaaa gctggtaaaa aagggcttac gacacccatt ttacgtcaaa ctgcacgaca
     5941 tgcagttgcg tgcatagcca tgcagcgggt taaaatttat caggcgcgtt tccggcggtt
     6001 ttccgggtgg tttgttgccg gttttatccc gtagccgccg gaaatgccct cagaccgtct
     6061 gagcggtgcg cgtaatgcga cgttatggta aatcatgtga ttaatggtag tgataatcat
     6121 tatcactttt gctatattct tgtttctttt attgtaagga aatgtaagta tggcaatcac
     6181 gattaaatcc gccgttaatg tccgtagcag agtattaata tactctttac tactgtttac
     6241 tggttcagcg tatgcggctg atatagctga atcaacattc ctgactcgtg atatcggcaa
     6301 tggtctgtat gaaatgtctt ttgatggcaa aaataatagt ctctgggcag catcatcgtc
     6361 ttcattcgac agagataagg gccacggaat tgtttatcag ttagaaccat ccactctgaa
     6421 aacaaaatct gccgttaaaa tcgcacgtct tgggtttgcc acagcactgg atgaggaaca
     6481 tgagcgactt tatatcggca atactctgga cggctcagtg acggtcattg atactctaac
     6541 cggaaaagaa ctttcagtaa ttccgttaac tgagggacag gatgaggctc atttcagacc
     6601 gaccagagaa atggtcattg acaaaaaaaa tcagcgtctt tatgtctcca gcgccgcaga
     6661 taaaggggct ctgtgggtta ttgacatggc tgtcgggaag aaaattaaaa ccattgatgg
     6721 actggggcac gctgttactg gaattgcgtt aaataaaaat ggggataata tttatctggt
     6781 taacggggat ggtgagatta ttaacctgga tgccagtagc tatgaaataa aaaaacggtt
     6841 tgttgtagag cctgagaaaa aacacttttt ccttaatgta agtctggatg aaaaaaacgg
     6901 acgtgcattt attaccgacc cggatattcc cggggtgctg gttgttgatg taaactcggg
     6961 aaaaatacta cacagaattg atgttataaa ttcactggcg attttattta atcctttacg
     7021 gaacgaaatc tatatcacgc accgtaatgc aaaacaggtc agcattgtgg acagtaattc
     7081 ttattacgta aaaacaacga taaaaacccg cctgatgcca aacagtctgt ctttatcaca
     7141 tgacggtgaa aatctgtacg ttagtgtaaa acaagaaaaa aaagagatga gtactatgaa
     7201 ggattacata ctcaaaatcg aattatctaa aattgctatg gagccagaag aatgaataaa
     7261 tatttagggc tgagcatagt atatatgatg tatacatcat ctgttatggc tgatgattct
     7321 ggcgcagtta gtaaagataa aaatatttca ccagaagaaa cattaattgt tcatgggacc
     7381 gctgtttctg aaagcacaac agaaggaaca gattcataca caacccagtc agccaataca
     7441 gcatcaaaga tgaatctgtc attccgggat acacctcagt caactacagt cattacgcga
     7501 cgaagaatgg atgatgtagc agcaaaaaat attattgatg tggttaaaac cgctcctgga
     7561 ttatatgtca gtacagcaga aggttccgga agaccgaatt ttatgtccag aggatttaat
     7621 gctaatgtca tgtatgaagg atttagcctg ccgtggaatt catacattcc aagttcacaa
     7681 actagttcag ctatttttga ccgggtggag atagtccgcg gggcaacagg tcttgttcag
     7741 ggtgccggag acccttcagc cgcgataaat atgatacaca agcgtccgac ccggcaatat
     7801 caggccagca ctaacatcgg tgccggacgg tggaatgact accggggtgg ggttgatgta
     7861 tcaggaccac tgaatgacag cgcgacctta cgcggaaggg cagttgtgga tggcgaaaac
     7921 agtgagacct tcagggatag tgagaaacgc aggaataacg tcatttacgg aattatggag
     7981 gcagatctga ctgataatac cctacttacg attgggggat atcagcagta tgaatataac
     8041 aatcattggt ggtatgactt acctatttct ggtaccggac accaccttaa tcttcctcgc
     8101 tcgacgttta caggcaatga ctgggagttt gaaagaagta aaagtaaaac tgcatttgca
     8161 actctggagc agaagttcag taatagctgg aagctgaaat tttcaacgct gcaaaactgg
     8221 agaaatgctg acctgttagg gacggcaaca taccggcata aagatacccg acaaagtatg
     8281 tatcagcaag tatggggatc actgagggaa tataattcca aaagctatga cctgtcgctt
     8341 aatggggact atgctttatt tggtcgtgtt catcaattcg tggctggaac aactcagcaa
     8401 aaatacaccg ataaagatac aggttataca tattccccaa tatatattga gaatgttaat
     8461 atatttgatc atggttcaca ttcttcgaaa aaacccatga agatgtcagg tgatgcatcc
     8521 tacgacgtca ctgaacaaaa aagcgcctac gcagcaactc attttctact tagtgataac
     8581 tggaaaatat tgctgggtgg ccgtcttgac tggtatgaat atacaagtaa aggtacatcc
     8641 attaatacag gttataaagt taccagaaat atcaccaaat atgcaggttc aacatacaat
     8701 cttgacgatc atcataccat ttatgggagc tataccgata tattcaatcc acagtcaagc
     8761 accgacctca atggtaaagt gatcaagcct attgttggta agaattatga gacaggtatt
     8821 aagggtgagt attttaacgg cttgcttaat gcctctctgg cggtatttca ggtagaccag
     8881 gaaaatcgag ccaggctgct tcccgatcag acagggtgtg ttactaatgg caatcctcaa
     8941 tgctatgagg ctagcggtaa agtacgcaca aaagggatag atttcgaact tcagggcatg
     9001 gttacgccac tctgggaaat tggggtggga tatacatatg cagatacgcg atatataaaa
     9061 gatggagatc gtagtaatga aggcaagcga ttcaatactg ccgtgtcgcc acaaaattta
     9121 tttaagattt cgagcatgta cacctttgac ggaaaattaa caaactggcg agcaggagta
     9181 aacacgtact ggcaaagtaa aatatatgct aacataacag acacaaggga tggcaatccg
     9241 gttattaata aacaaaaggc ttattcaaca actgacatta ttattggcta tactccaatg
     9301 ccacaactgg atttacagct caatataact aatgtctttg ataaggttta ttatacatcc
     9361 ataggtgaat cgattggata tggcacggaa acatatggag aaccaagaaa tgttaaaatt
     9421 agtgcaacct atagattcta accatgcgga ttgggtcttc tcctcggatt tgcccctata
     9481 aatccgtctt ttgtggttcc ggcgtacaac cccctgtttg ccacttcagt tgatggttaa
     9541 tcaaatcatg tagaggatct tcttgagatc ctccatggtt cgtcgtaatc ttttgccctg
     9601 taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tgggtaactg
     9661 cttaaccgtg gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag
     9721 ccggactttg
//
