LOCUS       CP128674                9294 bp    DNA     circular BCT 03-JUL-2023
DEFINITION  Klebsiella pneumoniae strain OC1092 plasmid pOC1092_5, complete
            sequence.
ACCESSION   CP128674
VERSION     CP128674.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN35878173
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Kochan,T.
  TITLE     Klebsiella pneumoniae Clinical Isolates with Features of Both
            Multidrug-Resistance and Hypervirulence Have Unexpectedly Low
            Virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Kochan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2023) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. v0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 124.716956169756x
            Sequencing Technology :: Oxford Nanopore MinION/GridION, Illumina
            NovaSeq/MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/26/2023 13:30:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,788
            CDSs (total)                      :: 5,659
            Genes (coding)                    :: 5,493
            CDSs (with protein)               :: 5,493
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 78 of 166
            Pseudo Genes (incomplete)         :: 91 of 166
            Pseudo Genes (internal stop)      :: 21 of 166
            Pseudo Genes (multiple problems)  :: 22 of 166
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="OC1092"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pOC1092_5"
                     /geo_loc_name="USA: Chicago"
                     /lat_lon="41.8945 N 87.6208 W"
     gene            join(9168..9294,1..404)
                     /locus_tag="QU718_28890"
     CDS             join(9168..9294,1..404)
                     /locus_tag="QU718_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJT79237.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(432..611)
                     /locus_tag="QU718_28895"
     CDS             complement(432..611)
                     /locus_tag="QU718_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WJT79238.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            680..1057
                     /locus_tag="QU718_28900"
     CDS             680..1057
                     /locus_tag="QU718_28900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WJT79239.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(1119..1418)
                     /locus_tag="QU718_28905"
     CDS             complement(1119..1418)
                     /locus_tag="QU718_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WJT79240.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1408..1671)
                     /locus_tag="QU718_28910"
     CDS             complement(1408..1671)
                     /locus_tag="QU718_28910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WJT79241.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(1977..2396)
                     /locus_tag="QU718_28915"
     CDS             complement(1977..2396)
                     /locus_tag="QU718_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WJT79242.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            2799..4484
                     /locus_tag="QU718_28920"
     CDS             2799..4484
                     /locus_tag="QU718_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WJT79243.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4494..4751
                     /locus_tag="QU718_28925"
     CDS             4494..4751
                     /locus_tag="QU718_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WJT79244.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            4836..4985
                     /locus_tag="QU718_28930"
     CDS             4836..4985
                     /locus_tag="QU718_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WJT79234.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            6466..8430
                     /locus_tag="QU718_28935"
     CDS             6466..8430
                     /locus_tag="QU718_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WJT79235.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            8430..9161
                     /locus_tag="QU718_28940"
     CDS             8430..9161
                     /locus_tag="QU718_28940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WJT79236.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     BGC             1..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5089..5667)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8431..9158
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gttttgtcga ccgggcccag catctggcga tgatgaaatc catcgggatg tatgacgagc
       61 ggctcaacag cgacgaaaaa gccacgtact ttttcactgg taaaaatccg caatgggaac
      121 ggaacaaacc cacaggaatg aagctgtgtg attacctcta tcgcaaggtg gagcggttcg
      181 ccatcgagaa cggtgtgcag gtggttgatg ccgggaagtg ctcaccggag cagattaacg
      241 cgatgattga cgtggtgagt gagcggcacg gggaaagcgc ggtgaacgag aagctgaaag
      301 cggagctgat gcgggaatat aacagccgct attttaacgt gccggtacct gaactgccgg
      361 aggacaaacc tgcccgccgg tggtggccgt tcggcaaaaa ataaatgcca gcaccggcca
      421 tgccggtgct gttattcctc cccgagctta tcccgcatca cctgccagag tgtttccgca
      481 tcctcgtgta gccgctcgca catgtcggcc ggggtatcca tatccagctc attcagttta
      541 tcgagcaata gcagcgactg ctccttaata aatttagcca tattgagggc tgttttttcc
      601 tgttttgtca tgattctttc cttccgggca tggtgtgcgc ctgtcaggta tactgtctca
      661 aaaatagcgg ggtgagacaa tggcgtgggt gacggtgaga caggcgacgg aactgacggg
      721 taaagcccga agttccctgt atcgggatat ggcgaaaggc cgcgtatcat accgtacgga
      781 agctgatggc ggccgggtgg tggataccag cgagcttatc cgggtgtacg gtgagttaag
      841 acaggatgag actcacgagc gggacgagtt gagactgtcg ggtgagactg aaaaaatggc
      901 ctccgacgcg ttgatcgcgg agataaaagc actcagggag gaggtggccg ggctacggca
      961 ggaaatgcag tccatgagac tgctggaaca caaaccggag acagcagaga ccccggccgg
     1021 aacaccgcgc cggtggtggc cgtggggaaa acgctgacgc gctgtgcttg tccaaccggc
     1081 gacggccacg caatgaattg cgtccccgtc accggttatt agagaacaat atggggattc
     1141 tggcgctggc cgaccacggc cagaacccgg acttttctgg tctggtcatc aacatcatag
     1201 aggacatgct gtcccatcag actgatccgg cgaacgtcct cgccatagcg tgagtccact
     1261 tcatagagtc gcgggaagtc ggccagtttg tccgccgcag cgagaacccg gtcggtaacg
     1321 ttctgcgcat cccacaggga tgaattttcc gcgatatagt caaacagcgt cgttacatcc
     1381 tcgatggctc cctgcttcca ttccacgtca tacggcatcg cgcagttcct gataacggcc
     1441 atgcaggcgc tggcggagtt gttccgtggt catacccgga cgcgtgtcag cacgggcggc
     1501 ggtcactttg gcgcgaacct gatcttcata ctctgcctgg gccagttctt cgttagccag
     1561 tcgctgcaac agcgcagctt tgtccgggcc accttccggc agtatgcggg gcaggctttc
     1621 agccagttcc cgaagaaagt tggcgttttc gtagtgtgta gtgttgctca ttgcggagcc
     1681 tcccgggtta atgcggcaat atgaaggaag tttagcaggg ggcggtctga aagaatatgg
     1741 tcgtgtcagt caggctgatg aagcagaaag tcggtaaaaa agggcttacg gcatccattt
     1801 tacgtcaaaa catatgccat gcagttgcgt gcataggcat gcattaggat aaaatttatc
     1861 gggcgcgttt ccggcagttt tccggggggg ttgttgcctg ttttatcccg tagccgccgg
     1921 aaacgccctg agcccgtctg agcggtgcgc gtaatgacgc gttatggtaa atccacttac
     1981 tgtctgcctt catagccatc gagataaaca gctgaaatgg tgtcagccag tagccttgat
     2041 tttttcggtg tgaggtcttt gctgcggtaa tcggtacccg caaattgtgt tgtgccttgg
     2101 agtgagttta aaaattcctg actgtgaaac tgctgaatgc gttgctgcac atcactccgt
     2161 gattttccgg tcgctttatc ctttgcgccc atatcccaga actgccggaa aaaaggcacc
     2221 atcattcgtg ttgtgtcagc tttctgggtg gaaaaattgg gatcaaaacc gtcatctgaa
     2281 gcatatacgt aatgccgggt atgctgttcc acgctgccct ttggagcaca ggcagtgaga
     2341 gttgctgtcg ctgttatcat ggcgataaga cacagtttgc tgccggaacg tctcatgagc
     2401 gtctccttac ctttaattct tttttctcat tgagttatat atccgcaggc tatcttatca
     2461 tcagtttctg accgtgttca gtgaccgaac aacccgaacc agaccccccg tttcttttcc
     2521 tgtcccggag ggggctttct ggtatctcat ataatgattt cagtaaaaca tactgaggcc
     2581 ggagaacggc gccgtttgtg ctggtttttg ttgcatctgg aggggattgc gcatggtgtg
     2641 agtggctgtt tttttgatca ttttcacaaa aacgcaaatt tacagacttg acatgaaaaa
     2701 caaggcagcg taatttttat actgtatata aaaccagtgg ttatatgtac agtattttat
     2761 tttagtttat tgttttaaaa gtcaaagagg aaaagtatat gagtggcgga gacggacgag
     2821 gtccgggtaa ttcaggtctg ggacacaatg gtggtcaggc cagtgggaat gtgaacggta
     2881 catcaggtaa aggtggccct tcatcaggtg gtggtacgga tccaaacagc gggccgggct
     2941 ggggtacgac gcatacacct aacggggata ttcataacta taacccgggg gagtttggta
     3001 acggcgggag taaacccggt ggtaatggcg gtaacagcgg caatcatagc gggagctctg
     3061 gtggcggaca gtcttcggcc accgcgatgg ccttcggtct gcctgccttg gccactccgg
     3121 gggctgaagg actggcttta tccgtttccg gcgatgcgtt gtcggccgct gttgctgatg
     3181 tgctggctgc cctgaaaggt ccgtttaagt ttggtctgtg ggggattgcg atttacggag
     3241 tgctgccttc tgagatagca aaagatgatc cgaaaatgat gtcaaaaatc gtgacgtcat
     3301 tgccggcaga cacggtaacg gagacgccgg tcagctccct gccgctggac caggcgacgg
     3361 tcagcgtcac taaacgtgtg gcggatattg tgaaggacga gcggcagcat attgcggttg
     3421 tcaccggccg gccaatgagt gttcctgtgg tggatgcgaa accgacaaaa cgtccggggg
     3481 tattcagtgt gtcgattccg ggtctcccgt ctctgcaggt gagcgtacct aaaggtgttc
     3541 cggccgcgaa agccccgcca aaaggcatta ttgctgaaaa aggtgattca cgtccggctg
     3601 gttttacggc cggtggtaac tcccgtgaag ccgttattcg tttcccgaaa gagagcggac
     3661 agaaaccggt ctatgtgtcg gtgacggatg ttcttactcc ggcacaggta aaacagcgtc
     3721 tggaggaaga aaagcgtcgc cagcaggcat gggacgctgc tcacccggaa gaggggctga
     3781 aaagagagta tgataaagcg aaagctgagc tggatgctga agataaaaat attgcgacct
     3841 taaacagtcg cattgcatcg acagagaagg cgatccccgg tgcaagggct gctgttcagg
     3901 aagccgataa aaaggtaaaa gaggcagagg caaataaaga tgattttgtg acttataacc
     3961 cgcctcatga atatggctcc gggtggcagg atcaggttcg ctatcttgat aaggatattc
     4021 agaatcagaa tgagaaatta aaagctgccc agacatcttt aaacgaaatg aatgaatcct
     4081 tatccaggga taaggctgcg ctttccgggg cgatggagag ccggaaacaa aaggagaaaa
     4141 aagcgaagga tgcagaaaat aaattaaatg aggaaaagaa aaaacctcgt aagggaacta
     4201 aagattacgg ccatgattat tttcctgatc ccaagactga agatattaag gggttgggag
     4261 agttgaaaga gggtaaacct aaaaccccta aacaaggtgg tggtggtaag cgggcccgat
     4321 ggtatggtga taaaaagcgt aaaatttatg aatgggattc ccagcacggt gagcttgaag
     4381 ggtaccgcgc cagtgatggc gaacacctcg gggcattcga tccaaaaacg ggtaagcagg
     4441 ttaaagggcc ggatccaaaa cgaaacatta aaaaatatct ttaagaggta aatatggggc
     4501 ttaaattaca tattcattgg tttgataaga aaaccgaaga gtttaaaggt ggtgaatact
     4561 caaaagactt cggtgatgat ggttctgtca ttgaaagtct ggggatgcct ttaaaggata
     4621 atattaataa tggttggttt gatgttgaaa aatcatgggt ttcgatatta cagccacact
     4681 ttaaaaatgt aatcgatatt agtaaatttg attactttgt atcctttgtt taccgggatg
     4741 gtaactggta agcaaaagtt atcaaggatg atttgaaata cagacttccg gtgttcaggg
     4801 atgaataccg gggtttttaa atacagggaa ggtatatgaa aaaggtaaag gttgtttttc
     4861 tttttatatt gattttttca ggctttcttc tggtcgcatg tcaggcaaac tatatccggg
     4921 atgttcaggg tggaacggtg gcaccatcgt cctcctctga actgacgggg atcgcggttc
     4981 agtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tcttttgccc
     5041 tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa ggttaagtca gttggggaac
     5101 tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac caaatctgtc cttccagtgt
     5161 agccggactt tggcgcacac ttcaagagca accgcgtgtt tagctaaaca aatcctctgc
     5221 gaactcccag ttaccaatgg ctgctgccag tggcgttttg cgtgcttttc cgggttggac
     5281 tcaagtgaac agttaccgga taaggcgcag cagtcgggct gaacgggggg ttcttgctta
     5341 cagcccagct tggagcgaac gacctacacc gagccgagat accagagtgt gagctatgag
     5401 aaagcgccac acttcccgca agggagaaag gcggaacagg tatccgggaa acggcagggt
     5461 tggaacagga gagcgcaaga gggagcgatt catcggaaac ggtgatgatc tttaagtcct
     5521 gtcgggtttc gccactcctg actgattcat ggttgagcca ccggctccca cagatgcacc
     5581 gaaaaagcgt ctgtttatgt gaactcagtc aggagggcgg agcctatgga aaaacgccac
     5641 ctgcgtggcc ttgccgtttt gcctcacatg ttagtcccct gcttattcac ggaatctgtg
     5701 ggtaactttg catgtgtccg cagcgcccgc cgcagtctca cggccggagc gtagcgaccg
     5761 agtgagcgag gaggcgcaat ttccccggca cctacaagac ggacaccgga cgcgctgatg
     5821 tggcatattg tcccctgatt aagtctttat attccctgat aataaattac caggataggt
     5881 gaatcatatc ggtttttcgc tgaacatttt acgttaacct atctctgttg cttttttctc
     5941 cttacggaga aatacaggtc atccccgcga gggcgtggct gacctcaggg ggatatcccc
     6001 tgcttacgct ggctggacgc caccgacgca cggtctctcc ccctctttct ggctccgtcc
     6061 ggctggacgc cggccacgcc agaaattcac ccggcccccg tcaggctcac gcctgaccac
     6121 ccacaaaaat ccacggtgct ggacgcacct ggcgaagcag caaagctgct cctgattttg
     6181 cgggtggtca gccgctcgct atggcgaact ctgacggggg ccggaacacc ggaaaagacc
     6241 gcactggggt gccgcctttt cctctcacac gctggggcgt gctcgttgtt cctccggggc
     6301 tgcgcctttt ccgcatgggg cggaaagagg ctggcctccg tggtgaaagc tgaacgcaat
     6361 caccacggac gtgaccgccg ccgccatgcc ttggggcatg tccgcggctt gtgcagcggg
     6421 ggctgcacat cacgccggca aaccatcgtc tgaaggagag agaacatgtt taatacggat
     6481 tcgcttgcct ggaagtggca cattccggag ctggcccggc agatttatcc gtgggtcagc
     6541 tggctgtacc aggagtatta cggtctggcc ggggctttcc tagttatcgg cctgtttttc
     6601 ggtctggctg aactttgggc cggacggatg cgttttgtgg ggctgagtca taaggagcga
     6661 cgggagtatg ccgatcgcta taagcgcgat aataaacggc cagcgccatt ttcggtgctg
     6721 gcctcgctgg attcgcccgg gcggatggtc agggcacgtc gacatgtctg gaactacgcg
     6781 tactggctga ccggcgcggc cggtctggcc cttctggggg ggctggttgt cgccgtgttt
     6841 atcgagagct ggggagaaac cgtcctgcgt ccggacctct acgcaaacgc agattattac
     6901 ctgctgcagc cggctaacct gttcgggatt gcctggtgcg cgggcgggct ggctgtcggg
     6961 attgtgttgg cgcggctgct ggcgttcctg ctgcttgacg gtcattttgc ggctgctgac
     7021 gaggcggtga acgcgaaact gaatcaggag agccggcgca gcagccagcg gacaggagag
     7081 atgaccgacg tgcgccacct gcatttcggg gagccggtac cggttaatgc gctggccgat
     7141 ttttcctcgg agcaggcccg gcaggagcag gcggtgtttc tgggtaagga tgagcagggc
     7201 aagccggtgc tggttccgcg tgatacctgg cgcaagacca acattcagat cctcggcctg
     7261 cccgggagcg gcaagagcgt gatggccacc aacgcgctca ttcgctgcgt acgcgatttc
     7321 ggggacgcgg tggtgtactt cgaccccaaa ggggacgcat gggcgccgca cgtcttccgg
     7381 gcgcactgtc cggacttcac cctgctggat ctgcggccgg ggaaaccggc gcagctgaat
     7441 ctgttccgcg atctcgacca gtacgcgctg aaaaacctgc tgattgccgg gcttaacctg
     7501 ggcgagaccg gcgacgtggc ggaccactac cgcatcagcg agcagaaagc ggcgaagctg
     7561 attgcggagc agtttccgca gggggcaaac attcagcagg tgctggcggc ggcgtacgcg
     7621 ctgccggagg ccctgaaaaa ggacgtgaag gggttgatca ctaggctgga aaacgtcgct
     7681 gacctgagcg tcctgcagac ggacagtggc atcgatgtgg cggggattat caacggcggc
     7741 ggctgcctgt acgttatcgg ctcgatggac gacgaggcgg tgatccgtgt gcaaaaaatg
     7801 ctgtttgccc gctgcgcgca aatcatcatt gcgcgggacg agttccggga gtggccacac
     7861 gtcagcgtga tgctcgatga aattaaatat ctgctgtcga agtatgtact gaacgcactg
     7921 gggacgctgc gcagccggga ctgcaatctg ttgctcgcgc accagtcgct gggcgatttc
     7981 gggcagtgcg ggcaggatct gcccgctgaa tttgtgaaaa ccacggtact ggataacacg
     8041 ccgatccgct ggttttaccg ggcggccagc ctggagtccg cgcagtgggc ggcggggcag
     8101 accggggaaa tcagggtgga cgtggagcgc cgccgtgcca gccgggaggc ggggaacgtg
     8161 gagcatatca gcggggacag cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac
     8221 accctgcaac acctgccgga cgggttcgcg gtcatgaccg ggctgggtgt ggcccggatg
     8281 gcgttcagca gtccgctgcg ggttgagcgc cgggaaatcc cgttgcagcg tttcccggtg
     8341 ctggcgaaaa ccgatcccct ggcggagttc cggcccccgg ccgacggcgg cgggccgaaa
     8401 gcggatgatg attttgcggg gatttactga tggcgctgga gctgtacaac gtcagccacg
     8461 cgaaacgaca ggcgcggaac gcggagaaaa cccggctgac cctgcgctgg ttgcgggaag
     8521 agctgtgttc cacggcggag ctggttgcgc ggcggctggg gattgcggcg gtgcagccgg
     8581 tctaccgctt cctcgacagc ctggtggcga aagggctgct ggtccggacc aaatatccgg
     8641 tggacggccg tcaggtcagc gtgtgggggc tgacgccgca tggcgtggcg ttctcgttcg
     8701 acgaggacga accgctggcg gatgttattc cgtttcagcc gtcaagggtg tcggcagcac
     8761 atctgccgca ccggctggcc gtgcaatccc tgcgccttga tatggaagcc agaggggcca
     8821 ccggctggcg cattctgcac cgtctgagtc ttaaaggcat gaaagtgccg gacgccctgg
     8881 cggagctgga cggtaaaacg gtggcgtttg aggtggagcg caccgtcaag agccgtcgcc
     8941 ggtatcagga agtggtgtcc gggtatctgt tcaaccgcaa agctaacggc atcgatgaaa
     9001 tctggtacat ctgcccggac cgggcgacgc aggtcagggt gcagcgggca atcctgtcgg
     9061 tggatgaaat cgtgaaccca cagaccggcg aggcccgcaa aacggcagag ctggatcgtg
     9121 aacggttgtt tgcgtgcttt aaatttatga caacggagtg agggattatg gcgctgtaca
     9181 tggtatttat tgaagacgcg gaggaccgga acggcgagcg ctgcatcatg ccgaccagcg
     9241 agcatctgga agatatggtg gtgctcgcct ggcagaccgg ggcgtatgtt ttct
//
