LOCUS       CP128660                4437 bp    DNA     circular BCT 03-JUL-2023
DEFINITION  Klebsiella pneumoniae strain hvKP2 plasmid phvKP2_2, complete
            sequence.
ACCESSION   CP128660
VERSION     CP128660.1
DBLINK      BioProject: PRJNA788509
            BioSample: SAMN35878177
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Kochan,T.
  TITLE     Klebsiella pneumoniae Clinical Isolates with Features of Both
            Multidrug-Resistance and Hypervirulence Have Unexpectedly Low
            Virulence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Kochan,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2023) Microbiology & Immunology, Northwestern
            University, 303 E Chicago Ave, Ward 6-035, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: trycycler v. v0.4.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 223.684393238552x
            Sequencing Technology :: Oxford Nanopore MinION/GridION, Illumina
            NovaSeq/MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/26/2023 13:26:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,391
            CDSs (total)                      :: 5,269
            Genes (coding)                    :: 5,118
            CDSs (with protein)               :: 5,118
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 77 of 151
            Pseudo Genes (incomplete)         :: 74 of 151
            Pseudo Genes (internal stop)      :: 34 of 151
            Pseudo Genes (multiple problems)  :: 32 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="hvKP2"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="phvKP2_2"
                     /geo_loc_name="USA: NY, Buffalo"
                     /lat_lon="42.9047 N 78.8494 W"
     gene            complement(901..1323)
                     /locus_tag="QU746_26940"
     CDS             complement(901..1323)
                     /locus_tag="QU746_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WJT37592.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDAVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1323..1514)
                     /locus_tag="QU746_26945"
     CDS             complement(1323..1514)
                     /locus_tag="QU746_26945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WJT37593.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1995..2144)
                     /locus_tag="QU746_26950"
     CDS             complement(1995..2144)
                     /locus_tag="QU746_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJT37594.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            3051..3965
                     /locus_tag="QU746_26955"
     CDS             3051..3965
                     /locus_tag="QU746_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WJT37591.1"
                     /translation="MPNTVNYPQIIPFISKGKMDAIKNTFGNTLTDRECYGIYIWSQKA
                     SAAIYPLLQQLEVTLRNSIDKEATNVMGLNWWDGVYTDKTKPKWDDFIFNINKGRDRYK
                     KNYKKNNPSVLSSLIVVPHDDAIAHTDFYTWQAVLSDTFHTQNRKHVNKSLWPRLTYKV
                     LKGLDRKKTEKAARLDFINELNEIRNYRNRLSHNDCIWIKMNSNNLQSAVETVREKINK
                     IEDLIKTINPQIHSTLVKWGVFYHARRICSQREVEHHMGKNIVNCTNPTMSSILATIYS
                     WTDDGKLTSVVNDGKNDLAIHKF"
                     /name="Abi family protein"
     Typing          2300..2811
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.881"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgtgtaaca ccttcgctgt cgcattcagt gacaggcact agtttatcac aaaaaacgat
       61 aaaagtcaat ataaccaaca ctttaaaacc aaacatttta gctactcgaa acggtaattt
      121 taacgaagtc ccccccccct caatacaaac ccgaatatta agctatttac cataacatcc
      181 cttgcgtgca ccacctgaga ggccctcagg gcgtttctga cgggaaaccg gcaaaacaga
      241 caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg gcatgcatga
      301 gtatgcgcgc aagtgcatag cgcggaaatt cacgccattc agccggggta agtcctgttt
      361 ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg tggccgtgaa
      421 gcgttgccgg cgcagccgga aaaccggttt tcagcacgtt caggaatcgg gttcgtcgac
      481 ggccggcaat cacgttatgg caagtcgccg gacgactcac cacacgattc accggcgatt
      541 cgactcgtga cttgactcta gagtcaccac ggtcaaaaaa acgccaaatc gacgattcga
      601 ctctggagtc aagtcgtcat aagttactga tagtgtggag tgagtcgtcg atgactctat
      661 gacgagtcgt cggcaagtcg tacacgaatc accgacgact tggattgacc ttacagaagg
      721 taatactgcg taatttaata acaaccaact aactgatatt aatgagttat ctgcaaaaat
      781 ttaattttgc gtggggtgtg gatattttta gtgtgaggag cgttcagaga tctgacgatt
      841 tttgggaagg ttcacgaacg ccccggcatg gccggagagg aaaactcaag catggcggga
      901 tcaggatttt ttccagaatt tccaccacgg ggaatggggt ggtgaagaag gttcgtgctt
      961 gtgctccaga agctgcatag cctgacgaag ggaatcaata tgctcatctc gagccttaac
     1021 agccccttta agaccatcgt tttcagcttc aagtaaagcg attctatgtt gtaaatcatg
     1081 taaaccaacg ctgttaatac ctgtagattc tgcgtcactt tttacatcat attttacagc
     1141 atcgattaca ccatcaagag aaccaaacac tctaataagc tctgatgtat caataacttt
     1201 aatacccttc ccgtgcttta caacagaggt gtaacttaac ttaccactgt taatcatctt
     1261 gtaaatagta gggcgactaa ccttaacaag ccgtgctgct tcactaatag acactctggc
     1321 catcagttac catcctgtaa tgcatccagt ctcgaggaca gtgtacggaa aagatgctcc
     1381 gcatcatcgt ggagtttctc acaaaggtcg gcctcagcat cgagatcaag ctcgttaagc
     1441 ttctccagca ggagcagaga ctgattctgg atgaatttag ccatgttcag cgcagttttc
     1501 tgttgtttcg tcataaggga cctcagagca tcagtgctca gtgtaaaacc ggtaaggggg
     1561 tcggatgtac gaatggttca gagaacggat ctgaggtaat cctgtagtta ttttgtagtt
     1621 tatctgtagt catcgcacta aaaatcagga ttaagtcatc agcttttgta gtctttctgt
     1681 agtttacctg tagtccggaa aaataatccg ttcatgcgag catgaacgag gtcgttaatg
     1741 acgttttttt gtcaccgacg ccccttaacc tccgcaggtc acccggccag aatgataatt
     1801 gtttggccct taaatggtca tacacctaac gcagccgcga ttttcatcgc aactgcatga
     1861 attaaaaggt aaaaattgaa acctccgttt atttatgtaa tacgcaacgt attacaatca
     1921 cctgtaacca catgaaaata aagaaataaa atgtaatacc ggcgtattac cacgtacgcg
     1981 ggcaagcgaa aaagctataa cgcttcccgg ccgacaggtg gcccggaatc cacaggggcg
     2041 tgcaggttct cttcagtaac ctgcgccatt aaatcaaaaa ggtcatattc gccgggaagc
     2101 tgcgtatcag tcgaatccag ttgttcatca tattcagatg gcatggggtg atatccagta
     2161 gctgagaggt tgttagcggg tttcggggcg cagccctgaa ccagtcaaat agcacgtgcg
     2221 gagtgtatac gggctcaata tgctacggtg gagtaacacg ttatgaaagt gcgtcatgtg
     2281 acagaggaaa gtgcagcggc ggggcgtacg ggagaaaatg gaaacccggg gatatgacgc
     2341 ctcctcgctc actcagtcgc tgccgctcct gccgttcggc tgcggcgggc gttacctgca
     2401 cacggataag agcaaagaca tacggcatga caagcggggc agcaaaagaa tgcgccgctg
     2461 ccgtttttcc ataggctccg cccccctgac aagcatcaca gaatcggacg ctcaaatcag
     2521 tggtggcgaa acccgacagg actataaaga tatcaggcgt ttccccctga tggctccctc
     2581 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
     2641 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
     2701 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga
     2761 gtccaacccg gatagacgtg caaatgcgcc actggcaacg gccactggta agcaggatgt
     2821 gcgagagatg tatcgagagt tcttgaagtg gaggccttag tgaggctaca ctggaaggac
     2881 agatttggtg acatgtctcg cacaagactg ttaccacggt tcagaaactt gccggttaat
     2941 gaaccttcga aaaaccacct tgccgggtgg ttttttcgtt tcatactcta caactatgct
     3001 gagccatatg gcaacatcaa ttcataccta catatgaaga agcgatgccc atgcctaaca
     3061 ccgttaatta tccgcaaata atcccgttca tatcgaaagg gaaaatggat gctataaaaa
     3121 acactttcgg taatacctta acagacaggg aatgctacgg gatatatata tggtcgcaaa
     3181 aagcgtctgc tgcaatatac cctctcctac agcagttaga ggtaacgctt aggaactcaa
     3241 tagataaaga agcaacaaat gttatgggtc tgaactggtg ggatggagtc tatacagata
     3301 aaaccaaacc taaatgggat gattttattt tcaacataaa caaaggccga gatagatata
     3361 agaagaatta taaaaaaaac aacccatctg tactcagctc tttgattgtc gttccgcatg
     3421 atgacgctat agctcatact gacttttaca catggcaagc ggtcctaagt gacacatttc
     3481 acacgcaaaa tagaaaacac gtaaataaat ctctctggcc aaggcttact tacaaggtgc
     3541 taaaaggtct agacagaaaa aaaacagaga aagcagcaag gcttgatttt atcaacgaac
     3601 taaatgagat aagaaattac agaaacagat tatcgcacaa tgactgtata tggataaaaa
     3661 tgaactccaa taacttacag tccgcagtgg aaactgttag agaaaaaata aataaaattg
     3721 aagacttaat aaaaacaatt aaccctcaaa tacattcaac cctggttaaa tggggagttt
     3781 tctaccatgc gagaagaatt tgctctcaaa gagaggttga gcatcatatg gggaaaaaca
     3841 tagtaaactg caccaaccca actatgagct ctattttagc aaccatttac tcatggacgg
     3901 acgatggaaa gctaaccagc gtcgtgaatg acggtaaaaa cgaccttgcg atacataagt
     3961 tctagaagga gaaaaggccc gacatgctaa gcatgtcgag ccttttccaa gtcttttgcc
     4021 aatatcacct tacggcggag cgtggccttt attccattat aaataatgca taaaagcgaa
     4081 agctctaggc ttcattaaaa atgattctac atcacttact caaaaaagag caagacctta
     4141 tgtcacaagg gatcacacga gcagccgtaa atgacttaac ttgatgaaaa aaaacgtttt
     4201 taatctaact aaacttacct gcctggcttt aataaagagc ttaaaaatgg tacaatcaac
     4261 catccaagca attgttttat aaagtcaatt agcgttccat cagacaaaaa ataatggaga
     4321 gaaaggagaa tgaaaacaat gaaaacgttc tttaaggtta aaaatctttt aaaatcaatg
     4381 agatacttaa agcgtttcct gtcactgaat gcggcagtga aacgcgagtg cgcggtg
//
