LOCUS       CP127756                2473 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C222 plasmid pC222_1, complete
            sequence.
ACCESSION   CP127756
VERSION     CP127756.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360844
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C222
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/12/2023 14:43:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,750
            CDSs (total)                      :: 2,674
            Genes (coding)                    :: 2,594
            CDSs (with protein)               :: 2,594
            Genes (RNA)                       :: 76
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 80
            CDSs (without protein)            :: 80
            Pseudo Genes (ambiguous residues) :: 0 of 80
            Pseudo Genes (frameshifted)       :: 34 of 80
            Pseudo Genes (incomplete)         :: 41 of 80
            Pseudo Genes (internal stop)      :: 26 of 80
            Pseudo Genes (multiple problems)  :: 19 of 80
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C222"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC222_1"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2014-01-08"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 45"
     gene            complement(join(2071..2473,1..74))
                     /locus_tag="PCL53_13740"
     CDS             complement(join(2071..2473,1..74))
                     /locus_tag="PCL53_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WJC39689.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            complement(522..1256)
                     /gene="erm(C)"
                     /locus_tag="PCL53_13745"
     ARG             complement(526..1256)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1317..1376)
                     /gene="ermCL"
                     /locus_tag="PCL53_13750"
     CDS             complement(1317..1376)
                     /gene="ermCL"
                     /locus_tag="PCL53_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WJC39691.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     ARG             complement(523..1256)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(2062..2473)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.518"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tctatgactt ctccactttc ttcgtctatg agcctctgag agcctttata gactgttcca
       61 tatctttctt tcatcttttt ctcactcctt attttaaact attctaacta tatcataact
      121 gttctaaaaa aaaaagaaca tttgttaaaa gaaattagaa caaaatgagt gaaaaattag
      181 aacaaacaaa ttccttataa accttatcat ctcaacctat attaagattt tacctagttg
      241 aatcttcttt tctatataaa gcgtcggagc atatcagggg gttatctaac gtaaatgcta
      301 cccttcggct cgctttcgct cggcattgac gtcagatact gcaccccctg aacccccatg
      361 ctccaacagc aaaaaggaaa ctttttgctg cttttccgac gcttattcgc ttcgctcata
      421 tttatataga aaagaagtga atgcgcaaaa gacataatcg attcacaaaa aataggtaca
      481 cgaaaaacaa gttaagggat gcagtttatg catcccttaa cttacttatt aaataattta
      541 tagctattga aaagagataa gaattgttca aagctaatat tgtttaaatc gtcaattcct
      601 gcatgtttta aggaattgtt aaattgattt tttgtaaata ttttcttgta ttctttgtta
      661 acccatttca taacgaaata attatacttc tgtttatctt tgtgtgatat tcttgatttt
      721 tttctattta atctgataag tgagctattc actttaggtt taggatgaaa atattctctt
      781 ggaaccatac ttaatataga aatatcaact tctgccatta aaaataatgc caatgagcgt
      841 tttgtattta ataatctttt agcaaacccg tattccacga ttaaataaat ctcatcagct
      901 atactatcaa aaacaatttt gcgtattata tccgtactta tgttataagg tatattacca
      961 aatattttat aggattggtt tttaggaaat ttaaactgca atatatcctt gtttaaaact
     1021 tggaaattat cgtgatcaac aagtttattt tctgtagttt tgcataattt atggtctatt
     1081 tcaatggcag ttacgaaatt acacctctgt actaattcaa gggtaaaatg cccttttcct
     1141 gagccgattt caaagatatt atcatgttca tttaatctta tatttgtcat tattttatct
     1201 atattatgtt ttgaagtaat aaagttttga ctgtgtttta tatttttctc gttcattata
     1261 accctcttta atttggttat aatgaatttt gcttattaac gattcattat aaccacttat
     1321 tttttgtttg gttgataatg aactgtgctg attacaaaaa tactaaaaat gcccatattt
     1381 tttcctcctt ataaaattag tataattata gcacgagctc tgataaatat gaacatgatg
     1441 agtgatcgtt aaatttatac tgcaatctga tgcgattatt gaataaaaga tatgagagat
     1501 ttatctagtt tcttttttta caagaaaaaa gaaagttctt aaaggtttta tacttttggt
     1561 cgtagagcac acggtttaac gacttaatta cgaagtaaat aagtctagtg tgttagactt
     1621 taatgttttt ttaaggcatt agtgcattta agcgtcagag catggcttta tgccgagaaa
     1681 actattggtt ggaatggcgt gtgtgttagc caaagctttg gcgagttggt tgggggtttc
     1741 atgggattaa tcccatgaaa gtaccaactc aacaacacac taacgcctgt tggttccaac
     1801 caataggaaa ttggaataag caattagtat aatgagagta taatgttggt ataacgttag
     1861 tataatgatg ctttttttca ttatattttt tatgtacttt aaacctgcac gcttatgtga
     1921 attagaaaaa gcttaatcgc atttcataga ttgacctccc aataactacg tggtgttatt
     1981 gggaggtcaa tctatttcat ttgcgttaag cttttgatct acattaagcg tgcaggtttt
     2041 ttgtctattt gtagacgatt ttatgttata ctagtccttg aaagaataat aatcagataa
     2101 tgcattttct tgtttttcat ttgcctcttg ctcaaagttc ccaaattcga gtaagaggta
     2161 tttttgtttt tggtcgtcgc ctctcattag tagttcaggg tttaacatta atactccagt
     2221 ttttcttttt ataatatttc cttcttctaa gattttaagt gttgttatta ctgtttgtag
     2281 acttgttcct gtagcttttg ctatttctct tgttgtagct atcattgtat tgttacttaa
     2341 gtggacatta tctaggatat agttaacgat tttaagtttt tttccgccaa tcatatctaa
     2401 catacttatt aattgcacta tatatgcctt tacgaagtta ccagacgttt gtttacggta
     2461 taacttgtct acc
//
