LOCUS       CP127724               20657 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C255 plasmid pC255_1, complete
            sequence.
ACCESSION   CP127724
VERSION     CP127724.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360862
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20657)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20657)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C255
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 29x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 03:47:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,717
            CDSs (total)                      :: 2,640
            Genes (coding)                    :: 2,573
            CDSs (with protein)               :: 2,573
            Genes (RNA)                       :: 77
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 34 of 67
            Pseudo Genes (incomplete)         :: 31 of 67
            Pseudo Genes (internal stop)      :: 19 of 67
            Pseudo Genes (multiple problems)  :: 16 of 67
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..20657
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C255"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC255_1"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2014-08-04"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 63"
     gene            166..842
                     /locus_tag="PCL78_13465"
                     /pseudo=""
     CDS             166..842
                     /locus_tag="PCL78_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            853..1135
                     /locus_tag="PCL78_13470"
                     /pseudo=""
     CDS             853..1135
                     /locus_tag="PCL78_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0003677 - DNA binding
                     [Evidence IEA]; GO_process: GO:0006260 - DNA replication
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            2345..2962
                     /gene="cadD"
                     /locus_tag="PCL78_13475"
     ARG             2346..2959
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            2981..3328
                     /locus_tag="PCL78_13480"
     CDS             2981..3328
                     /locus_tag="PCL78_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO_process: GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WJB81844.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3497..3589
                     /locus_tag="PCL78_13485"
     CDS             3497..3589
                     /locus_tag="PCL78_13485"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WJB81845.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3801..4775
                     /locus_tag="PCL78_13490"
     CDS             3801..4775
                     /locus_tag="PCL78_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WJB81846.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKTTLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(4895..5710)
                     /locus_tag="PCL78_13495"
     CDS             complement(4895..5710)
                     /locus_tag="PCL78_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WJB81847.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(5703..7146)
                     /locus_tag="PCL78_13500"
                     /pseudo=""
     CDS             complement(5703..7146)
                     /locus_tag="PCL78_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            complement(7118..7711)
                     /locus_tag="PCL78_13505"
     CDS             complement(7118..7711)
                     /locus_tag="PCL78_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WJB81848.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(7975..8355)
                     /gene="blaI"
                     /locus_tag="PCL78_13510"
     ARG             complement(7979..8355)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(8345..10102)
                     /gene="blaR1"
                     /locus_tag="PCL78_13515"
     ARG             complement(8349..10102)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            10209..11054
                     /gene="blaZ"
                     /locus_tag="PCL78_13520"
     ARG             10210..11051
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            11423..12031
                     /locus_tag="PCL78_13525"
     CDS             11423..12031
                     /locus_tag="PCL78_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WJB81852.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVIHTVNYLKDKEVQLMITSLPMMNEVVGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRI
                     IYHRVVEMLEEGQSISKIAKEVNITRQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            12445..12738
                     /locus_tag="PCL78_13530"
     CDS             12445..12738
                     /locus_tag="PCL78_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WJB81833.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            12813..14747
                     /locus_tag="PCL78_13535"
     CDS             12813..14747
                     /locus_tag="PCL78_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WJB81834.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            14751..15062
                     /locus_tag="PCL78_13540"
     CDS             14751..15062
                     /locus_tag="PCL78_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WJB81835.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            15074..15700
                     /locus_tag="PCL78_13545"
     CDS             15074..15700
                     /locus_tag="PCL78_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WJB81836.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            15938..16567
                     /gene="mobV"
                     /locus_tag="PCL78_13550"
                     /pseudo=""
     CDS             15938..16567
                     /gene="mobV"
                     /locus_tag="PCL78_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013258.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            16661..17206
                     /locus_tag="PCL78_13555"
     CDS             16661..17206
                     /locus_tag="PCL78_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="WJB81837.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="recombinase"
     gene            complement(17248..17532)
                     /locus_tag="PCL78_13560"
     CDS             complement(17248..17532)
                     /locus_tag="PCL78_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJB81838.1"
                     /translation="MKTFIHNKLLYYQCQTIKGVQLFKTLLLCGKNTPFMWQKHSFYVA
                     KTLLLCGKNTPLGIEIPCCSRAEQAPKKVFKKIYKNILKSSRGATYFSS"
                     /name="hypothetical protein"
     gene            17590..18450
                     /locus_tag="PCL78_13565"
     CDS             17590..18450
                     /locus_tag="PCL78_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WJB81839.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="replication initiation protein"
     gene            18537..18878
                     /locus_tag="PCL78_13570"
     CDS             18537..18878
                     /locus_tag="PCL78_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WJB81840.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(19045..19116)
                     /locus_tag="PCL78_13575"
     CDS             complement(19045..19116)
                     /locus_tag="PCL78_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WJB81853.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            19265..19450
                     /locus_tag="PCL78_13580"
     CDS             19265..19450
                     /locus_tag="PCL78_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJB81841.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(19669..20412)
                     /locus_tag="PCL78_13585"
     CDS             complement(19669..20412)
                     /locus_tag="PCL78_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WJB81842.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEPKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             10210..11054
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             7445..17738
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1193..1340
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          17591..18450
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19670..20412)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15939..16213
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 ttggtaataa aacgtttaga aactgtttat ttatataatt ttcgatactt agattatata
       61 atgaataata tcttagtgca agcacctaga ttattaaata aacacaaaaa atgtaagcgt
      121 tttattacct cttaccaaac taaaattcgg aaagaggttt ttattatgtc aattaaactt
      181 tacaacataa atgaggaata cagagaaaag ttttatcaaa taccgaaagt attttttaca
      241 aatcctaaat atatcaagtt atccaatgat gcaaaaatgg catggggtat tcttagagat
      301 cgtttagatt tatcaattaa aaatggttgg gttgattcta aaacaggtaa tatttatttt
      361 tattacaaaa atgaaaaccc acaaaatata ttaaattgta aaaaagataa agtaattaaa
      421 ataaagaaag aacttaattg aagccgaatt actactacaa gaaagacaag gtttaaataa
      481 gacaaataaa ctttatttag ttaaaccaga agtaacagaa gatgatattt ataaaattca
      541 aaagcaagaa aacactgttg agagcagcaa tggcaaggaa gtcggaaaaa ccgacttcaa
      601 gaagtcgata aaacagaatc taatgatact gaacttagct atactgaatc taacaatact
      661 gaaataaatg atatgaatga cttgaatgat atagagtccg aaaatgaaat tagtaatcat
      721 tcaagtcaat gcaaggttat ttaatgcaaa caattttaac tgaatttgaa gaaacacatg
      781 cactttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa tcattcaaat
      841 gattttataa caatcaaaga tacatcaaaa tatttcgtta aatcatctga ctttttaact
      901 gatagcgatg aaaaaatcaa gaaattgtca gtgatctaga aaaagatttg tatcgaaaac
      961 gtatgttaag ttatggtgga ttgcttaaat aaaaacataa aattttaaac ttagacgatg
     1021 ctgaagatgg caatttaatt aatacaagtg atgaagataa aatagcaaat gaagaagaaa
     1081 aggcacattc gattacggca attttgaaaa gcaaaattat tatttaaggc attagtgata
     1141 actgatgtct ttttttatta cttttttgta aaaaaagtgc tgtcttattt ttgtgacaaa
     1201 tgctgtatgt agtgtcacaa aagtgtgaca cttgagcttt ttatgacccc acacaaaaac
     1261 attttcgaag ccttgaaata tctggctttg ccagatcccc cattcattga tggggacaaa
     1321 tttcccttat gctcttacgg agattttaga gttaaattaa aatttctaag tttttgataa
     1381 gaaaagccct gcaggaattt agaagaacgt ggggaagttt taaagaattt tatgctaatt
     1441 tttaatttgc atgtaaatgg gcagtgtcta aaaaatagtt attgtttttg ctcaaatttt
     1501 tgtttttgaa aatagcatat atttatttgg ctcatatttg cgttttaaaa gcgtatataa
     1561 gttttagata taaaactata tgaatcaccc ctaaatcttt aaaatgccct cttaaaattc
     1621 aaaataaggg cataaaaaat aaaagccccc cagataaagt gaggtagctt tttctttcga
     1681 cgccgtatcg tcgatgtaat tcaaaaaagt tatgggctat aaatctacat cactttttca
     1741 agaatgtagt agcaatattc aacgcaaatt aatttttata actgctcgga atatctcaag
     1801 ccgtttcttt aattttgaaa taaaaaaatc gacgaaggtc gattacgttt ttgcacatgt
     1861 cgatttatcc gacgtataag tgcgccctta cgggatttaa ctagattata acgacgaatt
     1921 ttagacctgt aaagcaataa caattaggtc taccaggtag ataaagtgta caaagtagaa
     1981 ccgaaaaagt tatttatttt gaagattttt cgtagatata atttgctaat aaacttccta
     2041 aaactgaata aagaaaaata gttaaactca tgtaatcact ccataattct gaagtgtctc
     2101 catgtctacg catagacacg catttatgcc gagaaaattt attgtgcgtt gagaagaacc
     2161 cttaactaaa cttgcagacg aatgtcggca tagcgtgagc tattaagccg accattcgac
     2221 aagttttggg attgttaagg gttccgaggc tcaacgtcaa taaagcaatt ggaataaagc
     2281 acctatgatt atttaaaaat ataagttata atacattcaa aaatattttt gaatgaggtg
     2341 tattatgatt caaacggttg ttgctgctgc tgttctttat attgctacag cagttgattt
     2401 attagtaatt ttattaatat tttttgctag agcaaagact agaaaagaat atagaaatat
     2461 ttatgttggt caatatttag ggtctattat tttaatatta gttagtttgt ttttagcttt
     2521 tgtattaaat tatgttccag aaaagtggat attaggttta ttgggtttaa taccaattta
     2581 tcttggaatt aaagtggcta tttatgatga ttgtgaagga gaaaagagag ctaaaaaaga
     2641 attgaatgaa aaaggattgt ctaaattagt tggtacggtt gcaattgtta cgatagcaag
     2701 ttgtggcgcc gataatattg gtttatttgt tccgtatttt gtgacattaa gtgttactaa
     2761 tttattactt actctgtttg tctttttaat tttaattttc ttcttggtgt ttactgcaca
     2821 aaaattagct aatatcccag gaattggaga aattgttgag aaatttagtc gttggattat
     2881 ggctatcatt tatatagctt taggtttatt tattattatt gaaaatgaca ctattcaaac
     2941 aattttagga tttatatttt aatttagggt gtgattttat atgagttatg aaaatacttg
     3001 tgatgtgatc tgtgtacatg aggataaagt taacaatgct ttaagttttt tagaagatga
     3061 taaatctaag aaattactta acattttaga aaaaatttgt gatgagaaga aattgaaaat
     3121 catattatct ttgattaaag aagatgagtt gtgtgtttgt gatatttctt tgatattgaa
     3181 aatgagtgtt gcttcaactt cacatcattt aaggctttta tataaaaatg aggtacttga
     3241 tttttataaa gatggaaaga tggcatatta ttttattaaa gacgatgaaa taagagagtt
     3301 tttctctaaa aatcaggagg gtttttgaaa cgagtgaaac gagtttcttc ttgtcttgat
     3361 actataaaaa acaaagttat ataacataag acgacttatc ggaagttatc tatcatcgta
     3421 tattcggaga tgatataata tacaggtcgc ctatctctca ggcgtcaatt cagacgcaga
     3481 gaggaggtgc attactttgc taatcatttt cgttcacatc atagcaccag tcatcagtgg
     3541 ctgtgctgtt gcgtattata cttattggct tagtaaacgc aacaaataat taataggcga
     3601 ctttataagc cagcatcgaa aaaatcccca cactattgca gtagtgtggg gattggtgca
     3661 ttaattgtta atcattttcg ttatctagat tataacactg gttagtagag gaatgcaaaa
     3721 agataatatt ttaagtagtc tttttggata ttatcatatg atatatttat gtaaattgat
     3781 tttttattaa aggagcatat atgagtaata ctaaaattaa atatcaaatt tttatttcat
     3841 caacttattt agatttaata gaagagcgtc aagcagtagt agaagcagtt ttaaacagta
     3901 ataatattcc agcaggtatg gaacttttta actcaagtag taaaagccaa tgggaaataa
     3961 taacacaatg gatcaatgaa tcagatgcag tgatattatt gttaggttct agttatggaa
     4021 caattgatag tgaagaaaat attagctata cagaaatgga gtacaatttt gctaaatctt
     4081 taggtaaacc tatatatgtt tttgagatga ataaagcatc taccgaatcc gcggaaaagt
     4141 ctaaaaaatt agatgagttt aagacaaaag ttaaagaaaa tagactaatt agtccaaata
     4201 ttaatgatat ttcggaatta aaaagtaaag tatctcagac tttaatgcaa agtgtaccta
     4261 gtgaaattaa agaaaatagt ggatggataa gagtacagga ttgtggtaaa aatgtaaaaa
     4321 aattaaaaaa attggatgct atacaaagag atttagatac atatgatttt ttgaaagaag
     4381 aactttataa aatattcatg tatgtaaaaa ctttttattt ccagcacaca ccatttgatg
     4441 atagttatat tgaagatata gaaaactacc tgctaaatta tgaaaataat ccacataaat
     4501 cattcatgaa aataaaagtt tatgattata aaaacttttt aaaacattta aaaaacatat
     4561 tgattttaat taaacaagga aaaaagccat tagctcaagc cggtcatttt aaattaagct
     4621 tttacgaaga ggatttaaaa ttgttacaaa aaaattataa gactacgcta gtcgatatta
     4681 aaggattgaa taaacatttt tataaggaat gtgataggtg cttaaaagca tataatattt
     4741 ttattgaggg tataaataaa catttatatg aatgatataa gtacacccta atcatacata
     4801 tataattgtt gtcactttac tcgatatatg agaatgactt tacctaataa atgagaaaag
     4861 caacgcacta tattaaaatg cgttgctttt tctattatcg aatacctata actaaactat
     4921 ctctagctgt ttctacaact tctgtactta ttttatctaa accatttata tccataattc
     4981 tgtctatctg cgcaaataaa cgatgaatca gcctaaaatt cccttttgta attttaataa
     5041 tcgtcgttat tgcctcataa tcagtaaagt cttcgagttt taaatcaaat cctaagtctt
     5101 gccacttata ttctaatata tgatgcgtct catctttact tagattatca aattcatgcg
     5161 caaaacctat acgcgaatat agttgaggat aacgagatag ttttttctct atacccggca
     5221 taccaataaa tatcattgct aaattatgtt catcataaat agctcttaat tgctctaaat
     5281 gttgcacttt aaggcgatca atctcatcta ctataattaa atcaatatat ttaaacatat
     5341 cactataatg tttactatgt tcatgtccat acttatttac aacatgtaat ttttgaccca
     5401 aatcaatgat tgcagtaata ctatataaat cattccttaa tttagtttgt ttttctgctg
     5461 gtgcattata aaatatttta tccaccgata gtatgtcatc ggtcgtttta ctagctaaat
     5521 cttcccaacc tctatggtta acttgttttt caatagtatc ccaatttgtg taatacctag
     5581 acgataaagt ttttccgaca ccaggttgac cataacatat accaatatac tgatatttaa
     5641 tacaagcatc acaaaattca gcaaagcgtt tatactcttt cgtttcaatg aaattttgat
     5701 ttttagtcat tacgatatct ctttaatttt ttgacgtttt tagtcgtttc ttgaggtgaa
     5761 taaccacaat ttttttcttc cttaattaat tccgtggtac ttggactagc aatattttgt
     5821 tttaagtgtt ttcttctctg actacgagcg tgctgtatgt cttttatatc tatggagtaa
     5881 tcagctattt ctggacatat agcagtacaa agaaattcat ctctataaaa cacacgtatt
     5941 tcagccatat catttgggtt ataacgaatc agtacatatt cacctacata agcttctaga
     6001 ttagtattac tgtatcgaaa accttgaaaa tgaatgccat ctgaatgaac ttttctagat
     6061 ttaggtattt ccaacaataa taaatcaagt tgttctaagc tacttggcat attagggaaa
     6121 aagtgattac tattccaatg actgataggc gtggtatgaa tagtactatg ttctttttga
     6181 ttataatctt caattaaaaa gtaacgtaat ttttcttcaa aattgtgaaa atttattaga
     6241 ttactaggag tatcattatt gtttatatat ccaggcagtt gttctaagaa agtttgatta
     6301 actgtctgaa agaatcgttc tatttttcca cgaccacgag gaacaccaac tttcgaaaac
     6361 atcaagttaa ttttcaaatc aatagcgact tgttccatat gatgtgaagt aaagtcactt
     6421 ccatgatctg tgtagaattt ttcgggtata ccacacactg gccaattggt atcattttta
     6481 ttccatattg cttggtgtag tgttaatgct gtgttttgag cattgggggg catcaaaact
     6541 taagaaatag cctgcaattg cacgactata gtcatccata ataatagtta gccatggtct
     6601 attgatatta cctttttggt ctaatataaa gatatctagc aaagtatggt ctgcttgcca
     6661 tatttcattg ggtctcgagg attctctaat ttgtattaaa tcgtacttat tttgatagta
     6721 tttttcaccc tgatgagaga aatcaataac agacttaggc atcgctttaa taacactata
     6781 aatttgttta taacttggta tatcaaaatt attttcttta cagtaattag tgattttcct
     6841 atgaattgtg gctatactat ttcttttatt cataagcgca aggcgttcaa tttcatcaat
     6901 tatctttggt tctattttac gtgttccttt atcagctctt gtcgcataga ttagtccttt
     6961 aagtccttgt tctttatatg acttattcca cctataaagg gtgcttaacg caatcccttt
     7021 acttttagaa atacttgata aagattgttc gcctaggata aaaggtttta taatattgta
     7081 cttttgagta gcttttttac gtttggcttc agaaaaatta gttagatact tctccttgtt
     7141 tttcatcttc cttgtcctcc aatttattga ggtaacgata aattgttgta cgtgaaacat
     7201 gccactgttc tgcaattgtc ttaattggcg tgccattatc ataaagggtc ttaagtaagt
     7261 ttaaatcttt ttgatttaat ttttctggtc tgcctccata tcggcctcta gcacgtgctg
     7321 caattcggcc agctgaagaa cgctctaaaa ttaaattacg ttcaaattct gcaaatgctg
     7381 cgaataaatg aaataataat tgtccagttg aacttgattt atccattgtt atattttctt
     7441 ctaaactatg gaaacttaca cctcgttcgt taagctcatt aactaatgtg attaaatcct
     7501 ccatattacg tcccaatcta tctaatctcc aaacaacaat cgtatctcct gacctcgcaa
     7561 attcaattgc tttatctaaa ccaggtcttt tactttttga accactcata tggtcattaa
     7621 atatcttttc acaaccatat gtatttaatc gatcctcctg taaatttaaa ttttgtaatc
     7681 cagttgaaac tcttgcataa cctattttca aaatacaaac ctcctatata catcttcagt
     7741 gtaccataac ttaattacaa ggtcttttat gaacatagat tttttgttgg gtttttgaac
     7801 acataatcct atgtaattct tacttattta gacataaaag aagagtgtac agaaaatggt
     7861 tattttctgt acactctcat ctttaataat atattttaaa tgtcattaac aaaactaatt
     7921 taataagagt caagcatagt ttacatatgt attatgtttg aaacatacct ctaattactt
     7981 tttactaata tcatttaaaa tatctcgcaa ttcttcaatt tctttgttat ttaattcttc
     8041 atttttcgca aaattcaaca ctaaactttt catatcccct ccatacagtt tattaagaaa
     8101 ggttttagca gttttcattt taatatcgtc ttctttaata tttgatgagt aaaaataaat
     8161 attttctgat ttgtatcgtt ttataatctc ttttttatat agtcttgtga ttaatgttct
     8221 aatcgtttta tcactaactt ctttatattt ttgaatttca actacaattt cattagctga
     8281 tactgatttt ttatcccata ttatattcat aacatcccat tcagccatag atatttcaac
     8341 ttgcttattg gccatttaaa acacccattc cttttaatat tttttcactt attaattcag
     8401 catttttccc agacgggttt ccatctgata aatgtgtagc aaaataatac ttatcatgat
     8461 ttgtaattac gtaacctaca aaccacccat tattatactt cccgtttact atacctgtac
     8521 ctgttttccc atacagttca tacttttcat ttttcttaat caataatgaa gaagataatt
     8581 gattctttgc ttttttacta aaatggttat tttgttccat catattttta aaaactatta
     8641 cttgttcaag attagatatt ttcaaactat cttccatcca atagctttta taacttccca
     8701 aattttcatt accataattt aattgcttga gttgagccgc agtatagttc tttggtattt
     8761 gattactaat acgttcgaag taccaattaa ctgaattttg cattgctgta tttaaatctt
     8821 gttccttatt ccaagactcg aaaagataat gtttatgatt ccatgacata cgtgaatttt
     8881 tatcacttat aatatgacgg tcaagtccaa acagtgctaa ataaattttg taagttgaat
     8941 caggagaata tctttttcta ctttcttttt cattataaat atagtatttg tctttcttca
     9001 tgctgtacat aacgaaagat ccactatttg aaccaaaatt tttactttca tctaaaattt
     9061 ggtaatcgct ttgtaagggc tttttataat tataatcagt taaggattgc cccattaaaa
     9121 actggctttg aattatcatg ataaaaaaag taaaaataca aataaaaatt agaattaatt
     9181 ttgactgctt tttcagatcg gcttctttta tattaattaa tctatttttg agtaatgatt
     9241 ttttaccatt aaatgaatgg cttaatatat ttttgttgaa aaatgaggtt tttaatacgg
     9301 agtccataat agattccgca taagttttaa attcattttt gttaatatta ttaaggacaa
     9361 atctatcggc ttctacttca ttgtcatgaa ttatctttct ttttacaata tgtactaatg
     9421 gattgtaact cataataata gagaagatat taaagataat taaatgtaaa gtatctctat
     9481 ttttagcatg agcatattca tgcagtatta tatattttag tcttttatca attacacttt
     9541 taaaatatga actaggaatc aaaataatat atttcccata ccaaaaagtt attggagatt
     9601 gaatagcctc tgcttttcga atcacaatat tttttttata ttgatggttg aaaagtatcg
     9661 tatctatttt atttttttca ttttcgttta gataaagtga ctgtttcttt aaatatttaa
     9721 gatataataa ggctttcaaa aatttaaaac ttaaaataat aactaaaact atccaaataa
     9781 ctgtgcagat attatcaatt gaatcccaat taaacttatg gatatctgtt gcgaactctt
     9841 gaacaggttt ggtggtaatt atgttatggt tcaagtcgtg tgacttactt tcaactgtgg
     9901 gcgcttgatt attcacatta ttaaatttta taaaagagaa tttaattggg ataaaaggaa
     9961 ttaatccagc aagcacagtt agataccaaa ctttataatt taaactataa ttaaaatagc
    10021 gttttaatat gtacctaaaa aatactaaca atagaaatat aaaacaaaag cttactacac
    10081 tcattattag taatttagcc attttgacac cttctttcaa atatttataa taaacaattg
    10141 acatcaatat tacaattgta atattattgt tttgtaaaaa ttacaactgt aatatcggag
    10201 ggtttatttt gaaaaagtta atatttttaa ttgtaatggc tttagtttta agtgcatgta
    10261 attcaaacag ttcacatgcc aaagagttaa atgatttaga aaaaaaatat aatgctcata
    10321 ttggtgttta tgctttagat actaaaagtg gtaaggaagt aaaatttaat tcagataaga
    10381 gatttgccta tgcttcgact tcaaaagcga taaatagtgc tattttgtta gaacaagtac
    10441 cttataataa gttaaataaa aaagtacata ttaacaaaga tgatatagtt gcttattctc
    10501 ctattttaga aaaatatgta ggaaaagaca tcactttaaa agaacttatt gaggcttcaa
    10561 tgacatatag tgataataca gcaaacaata aaattataaa agaaatcggt ggaatcaaaa
    10621 aagttaaaca acgtctaaaa gaactaggag ataaagtaac aaatccagtt agatatgaga
    10681 tagaattaaa ttactattcg ccaaagagca aaaaagatac ttcaacgcct gctgctttcg
    10741 gcaagacttt aaataaactt atcgcaaatg gaaaattaag caaaaaaaat aaaaatttct
    10801 tacttgattt aatgtttaat aataaaaacg gagacacttt aattaaagat ggtgttccaa
    10861 aagactataa ggttgctgat aaaagtggtc aagcaataac atatgcttct agaaatgatg
    10921 ttgcttttgt ttatcctaag ggccaatctg aacctattgt tttagtcatt tttacgaata
    10981 aagacaataa aagtgataag ccaaatgata agttgataag tgaaaccgcc aagagtgtaa
    11041 tgaaggaatt ttaatattct aaatgcataa taaatactga taacatctta tagtttgtat
    11101 tatattttct attatcgttg acatgtataa ttttgatatc aaaacctgat tttcccttta
    11161 ttattttcga gatttatttt cttaattctc tttaacaaac tagaaatatt gtatatacaa
    11221 aaaatcataa ataatagatg aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac
    11281 gtatcttatt taaagtgcgt tgcttttttc tcatttataa ggttaaataa ttctcatata
    11341 tcaagcaaag tgacataatt gttgaattta tagtaactga aacgtatgat tagggtgtat
    11401 attaattatg agatggaaat caatgattgt tggctatgcg agagtatcat cgaacgatca
    11461 aaatttagaa agacaacttg aaaatttaaa gacgttttgt gctgaaaaaa tatttacaga
    11521 aaaacagtct gggaaatcta ttgaaaatag accagtactt caagaagcac ttaacttttt
    11581 gaggatggga gatcggtttg tagtagaatc cattgattgt ttaggtcgta attatgatga
    11641 agtgattcat accgttaatt atttaaaaga caaagaagta caattaatga ttactagctt
    11701 acctatgatg aatgaagtgg ttggcaatcc attactggat aagtttatga aagatttaat
    11761 tattcaaata ttagcaatgg tttcagaaca agagagaaat gaaagtaaac gtcgacaagc
    11821 gcaaggaatt caagttgcga aggaaaaagg cgtatataaa gggcgcccct tactttattc
    11881 accaaatgct aaagatccac agaaacgtat tatttatcat cgcgttgtag aaatgttaga
    11941 agaaggccaa tcaattagta aaattgcgaa agaagtaaat ataacaaggc agacgattta
    12001 cagaattaaa aacgataatg atttgattta ataaactttg tttcaatgca actgagagct
    12061 tctcacagcg tctcaaaaca gatgattata atctattatg agtttttaat aataagacaa
    12121 gaaaagagag cgtaaatatg ctctctcttt ttctcattaa cgaatgttta taacgcaatc
    12181 atgatgagag tattaataca gttaattatt tgaaatactt tagtttatat aatgttgaat
    12241 tataggtagt gaaattgtat ttattataca ttgtcatatc gttgtttaat gatagttaat
    12301 ttaaggataa tagaattaat attatatata gatgaacaaa acaagcaaca gagcagaaaa
    12361 aacactttta gattattttt actcattctc tattttattt atttttgcta taataaataa
    12421 agaatattaa atggaggtgt ttttatgaaa aagaaatttg tttctagttg tattgctagt
    12481 acaatcttat tcggcacttt attaggtgta acttataaag ctgaagctgc aacagtacat
    12541 gtagctggtg gtgtttggag ccatggtatt ggaaaacatt acgtatggtc ttattatagt
    12601 cacaataaaa gaaatcatgg ttcaacagca gtagggaaat attcatcttt tagtggtgtt
    12661 gctcgacctg gtgttcaatc taaggcatct gctccaaagg cttggggtgg aaacaaaaca
    12721 ttttatagtc ttcactaatt acatatatta attaaggaga atagacagaa ttaagtaaac
    12781 ttaattctgt ctattctctt gaaagaggca atatgaaaaa attcaaatta tttttagatt
    12841 ttagtacttt attattaatt tcagggctat tatttttatt tttcttcaaa gaaaatgaag
    12901 aaattatacc tgaaagttcc aatattttaa caatctctaa ttgggataaa tctaacagta
    12961 aatcaaaagt gttagatgtc attgaatcag gcgccaaaaa tcaaaatata caaataatta
    13021 aatccgttaa agattttgat aataaaaaag aattttttgt tttcaactca aaacgaaata
    13081 attctgactt tatacgtaat aaaacatcac ttttaactcc atcagattta ttaaacagag
    13141 agataaaagg aaagtattat attattggtg aacatttcaa tgtagaagag cttacgagtg
    13201 aattaaaaaa agctggttta acatctgaag ttgaatacat aaatagaaac atattatttt
    13261 ttgaattagt catagataat gatttattag tgcctttcat attcttaggc gtattatatc
    13321 ttttgtactt cttatatgac agaggatata atttgaaatt ttatgctatt caaaagctac
    13381 atggcttttc aactacacgc attatttttc ataatattca tataaaaatc atttattggc
    13441 tagtacttac cgctattttc tttattgcta atatattaat cattcatata gccaatttag
    13501 aattggattt cttaatgttt attcgtagat ttatcgtgtt gctttttgtt tttgcttgta
    13561 ttactacatt gctgtggctg atttcgtata gtcttctttt gaagttaagt attccattaa
    13621 tcttaaaagg gaaaaaagga tataagtttt caatatttat gggtaccttc accaaatgta
    13681 tcatggtgct aatactatct tttttactgg cacaaaattt aaatgcttat acgaagttaa
    13741 agaatatata tgactctgaa aaaatctggc aaaagttaga taattactat acattagaga
    13801 tttctcctaa tattagaaat tcagaagaaa aacaaatttt agaaactaat atatacaatt
    13861 taattaagaa aagcgaacaa cttggtggat tgcttttaaa gaataataat atagccaatc
    13921 ccgataaaaa caattacatt cctgaaaatg gcaatgtctt ttttatcaat aacaattttc
    13981 tgaatttcta taaaaatata aatcatgatt ttaaaatgat tgacaatcat tcggataaaa
    14041 taaacgtgtt tattcctcca aggttacaat atcaaaaatc tgaaattcaa aaaaatcacc
    14101 aaggatggat tgactttcaa aagcaacaaa acaaaaaagt agatgtgcaa gtactgtcaa
    14161 aaaacgtatc tgttttttct tttgatcgca caactaattt gaaatttggg tatctagatt
    14221 ctccaatagt aatgttgttg acacttgacg atttaacagg agatttttat ttagctgctg
    14281 tttctcaagg tggatatcta tttaaagatt tagatacact taaacagttg cttaaagata
    14341 atcacctaga agaagaaatt agcggtataa ctaattacaa ggattctgtg ttaaatgaat
    14401 taaatgaaac gaaaacaaaa atgataataa caatagttac tattattatt aacgttttca
    14461 ttcttttgat agctactgta tttgaaacaa ttcaatattt tagctggaat aaaaagcaac
    14521 tattattaag aaaaattcat ggttattcat tattcagcag taatgtacgt tatttaacaa
    14581 tttctatctt actttctatt atgttagctt acactacgca tattttattt ggttcaaaga
    14641 tattactatt cataataatg tctattgcta ttattcaaca tttacttcaa attgcttata
    14701 ttaaatatct tgaaaaatac tttaaagatt taatgaggga gatttaaaca atgaaaagaa
    14761 ctttaattat tgtaacaatt gtactattaa tcatcatact tagtctgtta ttggttagaa
    14821 atgagcattt agatcgtttt aacccactgt taaaagaaaa agtatcttat gccaaagttc
    14881 caaaagatac ccaggactat agaaatataa ctgtatactc taaaaatgga gaaaaaaaga
    14941 gttataaatt ggatttttta ggttatgatc ctacaaaaga atatgtgaaa gtaaatcata
    15001 aagggaaata tgtgcgttcg atagaataca ttacaaaaga tattcctagc tttttaaaat
    15061 gaggtgaccg atagtgataa aacttgaaaa tgtttttgta aaaaaaggta ataaaaacat
    15121 tttagacgga tgtaacttta attttgaaaa aggaaaatca tatgctttag ttggagaaag
    15181 tggcgctgga aaatctactt tacttaacat aatcgctggg ttcgaagatg tatcacaagg
    15241 aagcatatat attgaagata agttactaaa aaagaaagtc gatttttata gatatacgtt
    15301 agggtatttg tttcaaaatt ttgctttact tgaaaatcaa acaatcagtc aaaatttaga
    15361 tttagcccta aagttcaaaa agaataaaaa agataatatg aacttaaaga aagaagtatt
    15421 aaaaaaggtc ggcctagatt tagatatcaa gagaattgtt agttctctaa gcggtggtga
    15481 acaacaaaga gtagcattgg caagattaat attaaaagat ccgaaaatca ttttagctga
    15541 tgaacctact gggtcactag atacgaaaaa tggaaaaata gttattgatt tattattgaa
    15601 gctacttgat gaaaataaaa ctatgattgt cgtaacgcat gatttagagc ttgcgaaacg
    15661 atttgatgtc atagtcaata tttctgagct tagaagttaa gaaatattga taggtatagc
    15721 catacataga gaaaaaacaa tatgaaatat gattagtcat tacttttgag ttttttaatt
    15781 taaggactgg attttccagt ccttttttat gatgacaaaa aacaagcctc gcagagcaca
    15841 cgtttttaat gacttatttg gtttttaata agtctagtat gttaaaactt ttctgtgttt
    15901 aggtgttata atcatatgca gtggaagttg atttattatg tcttattcaa ttattcgagt
    15961 tgttaaagtg aaatcaaaaa caaatacaag aggtattcaa agacatattc aaagagaaaa
    16021 taaaaattat gaaaatatag atattgattt aagtaagtca tatttaaact atgatttagt
    16081 caatgatact aagtttgatt ttaataaaaa aattgatgag aaaatcgaaa aaaattataa
    16141 agggaaacgc aaaattagaa cagatgccat taaacatatt gatggattaa ttacatcaga
    16201 taatgtgttt tttaatcaat tatctgaaga agagacaaaa tagtttttta agcatgctaa
    16261 agcgttcttg gaagaagaat acggtaaaga aaatctttta tatgcgacag tacatatgga
    16321 tgagataaca ccacatatgc attatggcgt agttcctatt actaaagatg gacgtttaag
    16381 tgcgaaagaa gttgtaggta ataaaaaagc attaaccgaa ttccaagata gattcaatac
    16441 gtatattaat aagcaaggct atgatttaaa acgaggaatt tctagacaac ttacaaaaga
    16501 aaaacatgat caagttagcc gttataaaca aaaaacagaa tatcataaac aaatgcatat
    16561 gcgctagaag caaatagagg attatttaaa gcaagaaaat gataaattga tacaaaacta
    16621 ccaaaaatca ttagatacgc ttaaaaagct tctgagtgtc atgtatgaaa ttaaaactaa
    16681 aaatgtaggt ggatggtttc ataaagagaa gcaagaaaca ggcaatattg taattactaa
    16741 aactgatttt gaaaaataca ctaaacaaat taaagcagca cagatgatat tagatgatta
    16801 tgaatgtatt aaaagtggga agtcattaaa aaaagcagag aaacaaaatg aaagtttagt
    16861 taatgaattg actagtgtcc atatggaaaa tgaaaagctg gttgaagaat ttaatgattt
    16921 agcacaacgt tataattatt tgttgtcaga gaatgaaaag aaagataaag aactaaatta
    16981 tacgcttaag ttattcaatc aagtttttaa aatcattaaa agtatgatga aagaagagcg
    17041 ttatcataca ctaataaatc atattgataa tcatttagac aattctaaaa tacgtgaagt
    17101 aatgacgata gataacaatg atgaacagtt ctttaagaag aaatatcaag cacaagaacg
    17161 agaaatcatt tttaaagaag atagagaaga tggttataca ttataaaaat agatatttaa
    17221 atgcatttgt ttgaggttaa ttatttttta ggatgaaaaa taggtagccc ccctcgaact
    17281 ttttaatata tttttataaa tctttttaaa aaccttttta ggggcttgtt cagccctaga
    17341 gcaacaaggg atttcaatgc ctaaaggagt gtttttgcca cataaaagga gtgtttttgc
    17401 cacataaaag gagtgttttt gccacataaa aggagtgttt ttgccacata aaaggagtgt
    17461 tttaaaaagt tgtactcctt ttatggtttg acattgataa taaagtagtt tattgtgaat
    17521 aaaagttttc aaatgggagt gtatattcaa acgatactcc tttaggttcg taataaaggg
    17581 ggctgtatca tgacaggaga gacagttgta tataaaaatg aaatgaattt agttccatta
    17641 aggcgattta cagctactga aattaattta ttttttgcga tgtgtaataa attaaaagag
    17701 caagatacta atacattacg tttgtctttt gatgaattaa aaaaattaag taattatagt
    17761 ccagaaacac gtaatattaa tagatttgct aatgatttag ataatgtgta taaaaagatg
    17821 ttgaatttaa ccattagata tgaagatgat gatgtaatag aaagattcgt tttattcaat
    17881 cattatagaa tccataagcg agaacaatat ctagaaatat caacttcttc aaatttaaaa
    17941 cacatattga attcaattac taataatttt acaaaatttg aattaaaaga aatgactagg
    18001 cttaaatcta catattctaa aaatatgttt agattactta aacaatacaa acatacaggc
    18061 tatttaaaaa ttcacatcga tgattttaaa aatcgtttag atatacctaa atcttatcgt
    18121 atgactgata ttaacaaaaa tgtttttaaa cctattatta ttgaattagg ttctatattt
    18181 aataatctta ctatcaataa aatcaaagcg aaaaaagggc gtaaaattga atggatagag
    18241 ttcacatttg acgctgagaa acgcattcat aacaaacgac aacctcaaat gtccaagata
    18301 gataaatcaa gacaatatgt gaggcgtgaa aagacgccta aatggttaga agaacggtca
    18361 tatgaaaagc aacctcaaaa agattacgac ccacaattag agaaagaacg agaggatttt
    18421 ttaaaacaac ttgaactgaa ttgggaataa cgcaactttt cgttgcgagt tggttaacta
    18481 aaagtatcgt gacttttcaa ttattttaat tatagtttga gttaaggagt gattttatga
    18541 aattagctga agctataaaa gaacagagag aattaaaagg atggtctcaa gaggaattag
    18601 ccaatatatt aaaggtttcc agacaaagtg tttctaaatg ggaaagtgcc aaaaactatc
    18661 cttccttaga tattttgatt gctatgagcg atttattcgg aatctcttta gagcatttaa
    18721 ttaaaggaga tgcacgtttt aaaaaagtta ttttagaggg aaattataga gagagtaaca
    18781 gggtaccttc catagttgat tttctttatg atttttggtg gcttttcttt cctgttgccg
    18841 gatttttagt ttgggccatt cattcgttca tgggttgatt ttaaaatcaa aatttaatgc
    18901 acatcaggca atatgaaata tatactaaaa gtaacaagaa tttcgtttgt aaacgcacaa
    18961 aaccccgaac tattcgccgt agttcggggt tttgtggtga attggcttta atcgtctata
    19021 ttatttttta tcacgtttat ggagctaata accaaataac gtaacgagac aaccactgat
    19081 tgccgttgtc atgatgtgaa caagaatttc attcataaat cgcacctcct ctctacgccg
    19141 aattgacatc tgagagatag acgaccttca cattataaca aatctctcct tccatgaaat
    19201 aacatgaata ttaataaatg ttgttatata ttaaagataa ataaatataa gggtgggatt
    19261 gataatgttt ggcgatcaaa gacaagaagc tacaaaatat gtcattaaag aaggttatca
    19321 agatatttat ttcctcaaca aaaacgggga atggtattat ttcgaagtaa gaagtgtctg
    19381 gcgtggcaaa catattatta gagttaaaga tggtttgtta ggttggagaa aagaaatagt
    19441 aactgaataa agatacaaaa tacttgccaa tgcaaaagaa tgaaaacgcg tcaaatcaac
    19501 gatgtataaa agatgcataa aagataatga aagtaaaaag ccataaatgc cgtcatatag
    19561 gcatttatgg ctttttgttt ggttaacata atcgatgatt atcagaagtt caattcacat
    19621 aaaaaagcac ggctaattta tgaacttgtc gtaccttttt aattaaaatt agttaccaaa
    19681 tagacgactc caaaacccct ttttaggttc cgtttcttca ctcttttctt ctatatcact
    19741 atcattgtca tttggattag ctgaaacgtc ttctctttgt gcttcttcac cttgtgtact
    19801 ttcgtcgctt tgacttccag atatatcctt atattgaatc tctgtttctt tagtttcttc
    19861 ctgatctcgg ttaatattta caggactagt agtatatgtt ttttcttgtg catcaacgtt
    19921 ttgtctagcg ttagtagatg tatcaaaggg ataattaagg tgtttttctt tttctaattg
    19981 attttctaat ttttgaatct ttttattact ctctaatgcc agaacttgct gattctcaag
    20041 caattttgat ctttcttgca actcttttct gtaaaattca attgttgttt catgacgact
    20101 ttcttgtttt tcaatttgat tatttaaagt attaatctga tttttaagta tttctactat
    20161 ttgagcatcg cttttagatt caaaattaat attatccgac tctttttttt gtttgtcttc
    20221 agtagcactt tcaaaaccat atttcttttt attttcattt actctttgaa tgattttttc
    20281 tatatcggta tcttctttaa taaacgaagt gttttcctgt tttattgtct ctatattcaa
    20341 tctcttaata ttattaaaaa tcgtttgctt actaacccct aattcttcac tcaacctttt
    20401 tacacttttc atatcatcaa ccctttataa aatgtttata aacccttagt aaaatattat
    20461 aagcttataa cgattaataa taaagtaatc gacaaataaa aatataaatt cgccaacctt
    20521 tttatcaagg gtttgtaaag ggtttatggt gtttatcaaa ggtttataaa cctatatttt
    20581 ccctttttta agtttattca aagtgctata ataaccttaa tataacaaaa accccaacta
    20641 ctggtaatag tcggggt
//
