LOCUS       CP127711               20735 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C21 plasmid pC21_1, complete sequence.
ACCESSION   CP127711
VERSION     CP127711.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360869
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20735)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20735)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 43x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 03:46:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,699
            CDSs (total)                      :: 2,618
            Genes (coding)                    :: 2,532
            CDSs (with protein)               :: 2,532
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 56 of 86
            Pseudo Genes (incomplete)         :: 28 of 86
            Pseudo Genes (internal stop)      :: 16 of 86
            Pseudo Genes (multiple problems)  :: 11 of 86
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..20735
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C21"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC21_1"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2011-09-26"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 70"
     gene            join(19959..20735,1..1006)
                     /gene="blaR1"
                     /locus_tag="PCL91_13245"
                     /pseudo=""
     CDS             join(19959..20735,1..1006)
                     /gene="blaR1"
                     /locus_tag="PCL91_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /name="blaR1"
     gene            996..1376
                     /gene="blaI"
                     /locus_tag="PCL91_13250"
     ARG             997..1373
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            1640..2233
                     /locus_tag="PCL91_13255"
     CDS             1640..2233
                     /locus_tag="PCL91_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WJB58398.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            2205..3647
                     /locus_tag="PCL91_13260"
     CDS             2205..3647
                     /locus_tag="PCL91_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="WJB58399.1"
                     /translation="MKNKEKYLTNFSEAKRKKATQKYNIIKPFILGEQSLSSISKSKGI
                     ALSTLYRWNKSYKEQGLKGLIYATRADKGTRKIEPKIIDEIERLALMNKRNSIATIHRK
                     ITNYCKENNFDIPSYKQIYSVIKAMPKSVIDFSHQGEKYYQNKYDLIQIRESSRPNEIW
                     QADHTLLDIFILDQKGNINRPWLTIIMDDYSRAIAGYFLSFDAPNAQNTALTLHQAIWN
                     KNDTNWPVCGIPEKFYTDHGSDFTSHHMEQVAIDLKINLMFSKVGVPRGRGKIERFFQT
                     VNQTFLEQLPGYINNNDTPSNLINFHNFEEKLRYFLIEDYNQKEHSTIHTTPISHWNSN
                     HFFPNMPSSLEQLDLLLLEIPKSRKVHSDGIHFQGFRYSNTNLEAYVGEYVLIRYNPND
                     MAEIRVFYRDEFLCTAICPEIADYSIDIKDIQHARSQRRKHLKQNIASPSTTELIKEEK
                     NCGYSPQETTKNVKKLKRYRND"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            3640..4454
                     /locus_tag="PCL91_13265"
                     /pseudo=""
     CDS             3640..4454
                     /locus_tag="PCL91_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /name="AAA family ATPase"
     gene            complement(4678..5376)
                     /locus_tag="PCL91_13270"
     CDS             complement(4678..5376)
                     /locus_tag="PCL91_13270"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WJB58404.1"
                     /translation="MNESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFE
                     MNKASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSG
                     WIRVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDI
                     ENYLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDL
                     KLLQKIIRLR"
                     /name="DUF4062 domain-containing protein"
     gene            complement(5388..5546)
                     /locus_tag="PCL91_13275"
     CDS             complement(5388..5546)
                     /locus_tag="PCL91_13275"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WJB58400.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMGLLTQ
                     VVKANGK"
                     /name="DUF4062 domain-containing protein"
     gene            complement(5758..5850)
                     /locus_tag="PCL91_13280"
     CDS             complement(5758..5850)
                     /locus_tag="PCL91_13280"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WJB58401.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(6019..6366)
                     /locus_tag="PCL91_13285"
     CDS             complement(6019..6366)
                     /locus_tag="PCL91_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO_process: GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WJB58402.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(6385..7002)
                     /gene="cadD"
                     /locus_tag="PCL91_13290"
     ARG             complement(6389..7002)
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            complement(8211..8493)
                     /locus_tag="PCL91_13295"
                     /pseudo=""
     CDS             complement(8211..8493)
                     /locus_tag="PCL91_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0003677 - DNA binding
                     [Evidence IEA]; GO_process: GO:0006260 - DNA replication
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(8504..9180)
                     /locus_tag="PCL91_13300"
                     /pseudo=""
     CDS             complement(8504..9180)
                     /locus_tag="PCL91_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            9589..10330
                     /locus_tag="PCL91_13305"
                     /pseudo=""
     CDS             9589..10330
                     /locus_tag="PCL91_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /name="DUF536 domain-containing protein"
     gene            complement(10550..10735)
                     /locus_tag="PCL91_13310"
     CDS             complement(10550..10735)
                     /locus_tag="PCL91_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJB58389.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            10884..10955
                     /locus_tag="PCL91_13315"
     CDS             10884..10955
                     /locus_tag="PCL91_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WJB58405.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(11194..11535)
                     /locus_tag="PCL91_13320"
     CDS             complement(11194..11535)
                     /locus_tag="PCL91_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WJB58390.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(11622..12482)
                     /locus_tag="PCL91_13325"
     CDS             complement(11622..12482)
                     /locus_tag="PCL91_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WJB58391.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="replication initiation protein"
     gene            complement(12864..13187)
                     /locus_tag="PCL91_13330"
     CDS             complement(12864..13187)
                     /locus_tag="PCL91_13330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="WJB58408.1"
                     /translation="MENEKLVEEFNDLAQRYNYLLSENEKKDKELNYTLKLFNQVFKII
                     KSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQAQEREIIFKEDREDG
                     YTL"
                     /name="recombinase"
     gene            complement(13203..14131)
                     /gene="mobV"
                     /locus_tag="PCL91_13335"
                     /pseudo=""
     CDS             complement(13203..14131)
                     /gene="mobV"
                     /locus_tag="PCL91_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /note="frameshifted; internal stop; incomplete; partial on
                     complete genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0003677 - DNA binding
                     [Evidence IEA]; GO_process: GO:0006310 - DNA recombination
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            complement(14369..14995)
                     /locus_tag="PCL91_13340"
     CDS             complement(14369..14995)
                     /locus_tag="PCL91_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WJB58392.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(15107..15316)
                     /locus_tag="PCL91_13345"
     CDS             complement(15107..15316)
                     /locus_tag="PCL91_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WJB58393.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKRVINWIF"
                     /name="YxeA family protein"
     gene            complement(15320..15835)
                     /locus_tag="PCL91_13350"
     CDS             complement(15320..15835)
                     /locus_tag="PCL91_13350"
                     /inference="COORDINATES: protein motif:HMM:NF018897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="WJB58394.1"
                     /translation="MLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEISG
                     ITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIHG
                     YSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEKY
                     FKDLMREI"
                     /name="DUF1430 domain-containing protein"
     gene            complement(15924..16712)
                     /locus_tag="PCL91_13355"
     CDS             complement(15924..16712)
                     /locus_tag="PCL91_13355"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJB58395.1"
                     /translation="MKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANILIIH
                     IANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSIFMG
                     TFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEKQIL
                     ETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHDFKM
                     IDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKK"
                     /name="hypothetical protein"
     gene            complement(16949..17251)
                     /locus_tag="PCL91_13360"
     CDS             complement(16949..17251)
                     /locus_tag="PCL91_13360"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJB58396.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFLFSTQNEIILTLYVIKHHF"
                     /name="hypothetical protein"
     gene            complement(17326..17619)
                     /locus_tag="PCL91_13365"
     CDS             complement(17326..17619)
                     /locus_tag="PCL91_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WJB58397.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            complement(18033..18641)
                     /locus_tag="PCL91_13370"
     CDS             complement(18033..18641)
                     /locus_tag="PCL91_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WJB58406.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVIHTVNYLKDKEVQLMITSLPMMNEVVGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRI
                     IYHRVVEMLEEGQSISKIAKEVNITRQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            complement(19010..19852)
                     /gene="blaZ"
                     /locus_tag="PCL91_13375"
                     /pseudo=""
     CDS             complement(19010..19852)
                     /gene="blaZ"
                     /locus_tag="PCL91_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /name="blaZ"
     ARG             complement(19011..19252)
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="97.5"
                     /coverage="28.47"
     BGC             12326..20735
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(8008..8154)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.667"
                     /coverage="100.0"
                     /evalue="4.9199999999999993e-23"
     Typing          9590..10330
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.597"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11623..12482)
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(13857..14131)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 cttagtctcg tgggctcgga gatgtgtata agagacagct attatggact ccgtattaaa
       61 aacctcattt ttcaacaaaa atatattaag ccattcattt aatggtaaaa aatcattact
      121 caaaaataga ttaattaata taaaagaagc cgatctgaaa aagcagtcaa aattaattct
      181 aatttttatt tgtattttta ctttttttat catgataatt caaagccagt ttttaatggg
      241 gcaatcctta actgattata attataaaaa gcccttacaa agcgattacc aaattttaga
      301 tgaaagtaaa aattttggtt caaatagtgg atctttcgtt atgtacagca tgaagaaaga
      361 caaatactat atttataatg aaaaagaaag tagaaaaaga tattctcctg attcaactta
      421 caaaatttat ttagcactgt ttggacttga ccgtcatatt ataagtgata aaaattcacg
      481 tatgtcatgg aatcataaac attatctttt cgagtcttgg aataaggaac aagatttaaa
      541 tacagcaatg caaaattcag ttaattggta cttcgaacgt attagtaatc aaataccaaa
      601 gaactatact gcggctcaac tcaagcaatt aaattatggt aatgaaaatt tgggaagtta
      661 taaaagctat tggatggaag atagtttgaa aatatctaat cttgaacaag taatagtttt
      721 taaaaatatg atggaacaaa ataaccattt tagtaaaaaa gcaaagaatc aattatcttc
      781 ttcattattg attaagaaaa atgaaaagta tgaactgtat gggaaaacag gtacaggtat
      841 agtaaacggg aagtataata atgggtggtt tgtaggttac gtaattacaa atcatgataa
      901 gtattatttt gctacacatt tatcagatgg aaacccgtct gggaaaaatg ctgaattaat
      961 aagtgaaaaa atattaaaag gaatgggtgt tttaaatggc caataagcaa gttgaaatat
     1021 ctatggctga atgggatgtt atgaatataa tatgggataa aaaatcagta tcagctaatg
     1081 aaattgtagt tgaaattcaa aaatataaag aagttagtga taaaacgatt agaacattaa
     1141 tcacaagact atataaaaaa gagattataa aacgatacaa atcagaaaat atttattttt
     1201 actcatcaaa tattaaagaa gacgatatta aaatgaaaac tgctaaaacc tttcttaata
     1261 aactgtatgg aggggatatg aaaagtttag tgttgaattt tgcgaaaaat gaagaattaa
     1321 ataacaaaga aattgaagaa ttgcgagata ttttaaatga tattagtaaa aagtaattag
     1381 aggtatgttt caaacataat acatatgtaa actatgcttg actcttatta aattagtttt
     1441 gttaatgaca tttaaaatat attattaaag atgagagtgt acagaaaata accattttct
     1501 gtacactctt cttttatgtc taaataagta agaattacat aggattatgt gttcaaaaac
     1561 ccaacaaaaa atctatgttc ataaaagacc ttgtaattaa gttatggtac actgaagatg
     1621 tatataggag gtttgtattt tgaaaatagg ttatgcaaga gtttcaactg gattacaaaa
     1681 tttaaattta caggaggatc gattaaatac atatggttgt gaaaagatat ttaatgacca
     1741 tatgagtggt tcaaaaagta aaagacctgg tttagataaa gcaattgaat ttgcgaggtc
     1801 aggagatacg attgttgttt ggagattaga tagattggga cgtaatatgg aggatttaat
     1861 cacattagtt aatgagctta acgaacgagg tgtaagtttc catagtttag aagaaaatat
     1921 aacaatggat aaatcaagtt caactggaca attattattt catttattcg cagcatttgc
     1981 agaatttgaa cgtaatttaa ttttagagcg ttcttcagct ggccgaattg cagcacgtgc
     2041 tagaggccga tatggaggca gaccagaaaa attaaatcaa aaagatttaa acttacttaa
     2101 gaccctttat gataatggca cgccaattaa gacaattgca gaacagtggc atgtttcacg
     2161 tacaacaatt tatcgttacc tcaataaatt ggaggacaag gaagatgaaa aacaaggaga
     2221 agtatctaac taatttttct gaagccaaac gtaaaaaagc tactcaaaag tacaatatta
     2281 taaaaccttt tatcctaggc gaacaatctt tatcaagtat ttctaaaagt aaagggattg
     2341 cgttaagcac cctttatagg tggaataagt catataaaga acaaggactt aaaggactaa
     2401 tctatgcgac aagagctgat aaaggaacac gtaaaataga accaaagata attgatgaaa
     2461 ttgaacgcct tgcgcttatg aataaaagaa atagtatagc cacaattcat aggaaaatca
     2521 ctaattactg taaagaaaat aattttgata taccaagtta taaacaaatt tatagtgtta
     2581 ttaaagcgat gcctaagtct gttattgatt tctctcatca gggtgaaaaa tactatcaaa
     2641 ataagtacga tttaatacaa attagagaat cctcgagacc caatgaaata tggcaagcag
     2701 accatacttt gctagatatc tttatattag accaaaaagg taatatcaat agaccatggc
     2761 taactattat tatggatgac tatagtcgtg caattgcagg ctatttctta agttttgatg
     2821 cccccaatgc tcaaaacaca gcattaacac tacaccaagc aatatggaat aaaaatgata
     2881 ccaattggcc agtgtgtggt atacccgaaa aattctacac agatcatgga agtgacttta
     2941 cttcacatca tatggaacaa gtcgctattg atttgaaaat taacttgatg ttttcgaaag
     3001 ttggtgttcc tcgtggtcgt ggaaaaatag aacgattctt tcagacagtt aatcaaactt
     3061 tcttagaaca actgcctgga tatataaaca ataatgatac tcctagtaat ctaataaatt
     3121 ttcacaattt tgaagaaaaa ttacgttact ttttaattga agattataat caaaaagaac
     3181 atagtactat tcataccacg cctatcagtc attggaatag taatcacttt ttccctaata
     3241 tgccaagtag cttagaacaa cttgatttat tattgttgga aatacctaaa tctagaaaag
     3301 ttcattcaga tggcattcat tttcaaggtt ttcgatacag taatactaat ctagaagctt
     3361 atgtaggtga atatgtactg attcgttata acccaaatga tatggctgaa atacgtgtgt
     3421 tttatagaga tgaatttctt tgtactgcta tatgtccaga aatagctgat tactccatag
     3481 atataaaaga catacagcac gctcgtagtc agagaagaaa acacttaaaa caaaatattg
     3541 ctagtccaag taccacggaa ttaattaagg aagaaaaaaa ttgtggttat tcacctcaag
     3601 aaacgactaa aaacgtcaaa aaattaaaga gatatcgtaa tgactaaaaa tcaaaatttc
     3661 attgaaacga aagagtataa acgctttgct gaattttgtg atgcttgtat taaatatcag
     3721 tatattggta tatgttatgg tcaacctggt gtcggaaaaa ctttatcgtc taggtattac
     3781 acaaattggg atactattga aaaacaagtt aaccatagag gttgggaaga tttagctagt
     3841 aaaacgaccg atgacatact atcggtggat aaaatatttt ataatgcacc agcagaaaaa
     3901 caaactaaat taaggaatga tttatatagt attactgcaa tcattgattt gggtcaaaaa
     3961 ttacatgttg taaataagta tggacatgaa catagtaaac attatagtga tatgtttaaa
     4021 tatattgatt taattatagt agatgagatt gatcgcctta aagtgcaaca tttagagcaa
     4081 ttaagagcta tttatgatga acataattta gcaatgatat ttattggtat gccgggtata
     4141 gagaaaaact atctcgttat cctcaactat attcgcgtat aggttttgcg catgaatttg
     4201 ataatctaag taaagatgag acgcatcata tattagaata taagtggcaa gacttaggat
     4261 ttgatttaaa actcgaagac tttactgatt atgaggcaat aacgacgatt attaaaatta
     4321 caaaagggaa ttttaggctg attcatcgtt tatttgcgca gatagacaga attatggata
     4381 taaatggttt agataaaata agtacagaag ttgtagaaac agctagagat agtttagtta
     4441 taggtattcg ataatagaaa aagcaacgca ttttaatata gtgcgttgct tttctcattt
     4501 attaggtaaa gtcattctca tatatcgagt aaagtgacaa caattatata tgtatgatta
     4561 gggtgtactt atatcattca tataaatgtt tatttatacc ctcaataaaa atattatatg
     4621 cttttaagca cctatcacat tccttataaa aatgtttatt caatccttta atatcgacta
     4681 gcgtagtctt ataatttttt gtaacaattt taaatcctct tcgtaaaagc ttaatttaaa
     4741 atgaccggct tgagctaatg gcttttttcc ttgtttaatt aaaatcaata tgttttttaa
     4801 atgttttaaa aagtttttat aatcataaac ttttattttc atgaatgatt tatgtggatt
     4861 attttcataa tttagcaggt agttttctat atcttcaata taactatcat caaatggtgt
     4921 gtgctggaaa taaaaagttt ttacatacat gaatatttta taaagttctt ctttcaaaaa
     4981 atcatatgta tctaaatctc tttgtatagc atccaatttt tttaattttt ttacattttt
     5041 accacaatcc tgtactctta tccatccact attttcttta atttcactag gtacactttg
     5101 cattaaagtc tgagatactt tactttttaa ttccgaaata tcattaatat ttggactaat
     5161 tagtctattt tctttaactt ttgtcttaaa ctcatctaat tttttagact tttccgcgga
     5221 ttcggtagat gctttattca tctcaaaaac atatataggt ttacctaaag atttagcaaa
     5281 attgtactcc atttctgtat agctaatatt ttcttcacta tcaattgttc cataactaga
     5341 acctaacaat aatatcactg catctgattc attgatccat tgtgttatta tttcccattg
     5401 gcttttacta cttgagttaa aagtcccata cctgctggaa tattattact gtttaaaact
     5461 gcttctacta ctgcttgacg ctcttctatt aaatctaaat aagttgatga aataaaaatt
     5521 tgatatttaa ttttagtatt actcatatat gctcctttaa taaaaaatca atttacataa
     5581 atatatcata tgataatatc caaaaagact acttaaaata ttatcttttt gcattcctct
     5641 actaaccagt gttataatct agataacgaa aatgattaac aattaatgca ccaatcccca
     5701 cactactgca atagtgtggg gattttttcg atgctggctt ataaagtcgc ctattaatta
     5761 tttgttgcgt ttactaagcc aataagtata atacgcaaca gcacagccac tgatgactgg
     5821 tgctatgatg tgaacgaaaa tgattagcaa agtaatgcac ctcctctctg cgtctgaatt
     5881 gacgcctgag agataggcga cctgtatatt atatcatctc cgaatatacg atgatagata
     5941 acttccgata agtcgtctta tgttatataa ctttgttttt tatagtatca agacaagaag
     6001 aaactcgttt cactcgtttc aaaaaccctc ctgattttta gagaaaaact ctcttatttc
     6061 atcgtcttta ataaaataat atgccatctt tccatcttta taaaaatcaa gtacctcatt
     6121 tttatataaa agccttaaat gatgtgaagt tgaagcaaca ctcattttca atatcaaaga
     6181 aatatcacaa acacacaact catcttcttt aatcaaagat aatatgattt tcaatttctt
     6241 ctcatcacaa attttttcta aaatgttaag taatttctta gatttatcat cttctaaaaa
     6301 acttaaagca ttgttaactt tatcctcatg tacacagatc acatcacaag tattttcata
     6361 actcatataa aatcacaccc taaattaaaa tataaatcct aaaattgttt gaatagtgtc
     6421 attttcaata ataataaata aacctaaagc tatataaatg atagccataa tccaacgact
     6481 aaatttctca acaatttctc caattcctgg gatattagct aatttttgtg cagtaaacac
     6541 caagaagaaa attaaaatta aaaagacaaa cagagtaagt aataaattag taacacttaa
     6601 tgtcacaaaa tacggaacaa ataaaccaat attatcggcg ccacaacttg ctatcgtaac
     6661 aattgcaacc gtaccaacta atttagacaa tcctttttca ttcaattctt ttttagctct
     6721 cttttctcct tcacaatcat cataaatagc cactttaatt ccaagataaa ttggtattaa
     6781 acccaataaa cctaatatcc acttttctgg aacataattt aatacaaaag ctaaaaacaa
     6841 actaactaat attaaaataa tagaccctaa atattgacca acataaatat ttctatattc
     6901 ttttctagtc tttgctctag caaaaaatat taataaaatt actaataaat caactgctgt
     6961 agcaatataa agaacagcag cagcaacaac cgtttgaatc ataatacacc tcattcaaaa
     7021 atatttttga atgtattata acttatattt ttaaataatc ataggtgctt tattccaatt
     7081 gctttattga cgttgagcct cggaaccctt aacaatccca aaacttgtcg aatggtcggc
     7141 ttaatagctc acgctatgcc gacattcgtc tgcaagttta gttaagggtt cttctcaaca
     7201 cacaataaat tttctcggca taaatgcgtg tctatgcgta gacatggaga cacttcagaa
     7261 ttatggagtg attacatgag tttaactatt tttctttatt cagttttagg aagtttatta
     7321 gcaaattata tctacgaaaa atcttcaaaa taaataactt tttcggttct actttgtaca
     7381 ctttatctac ctggtagacc taattgttat tgctttacag gtctaaaatt cgtcgttata
     7441 atctagttaa atcccgtaag ggcgcactta tacgtcggat aaatcgacat gtgcaaaaac
     7501 gtaatcgacc ttcgtcgatt tttttatttc aaaattaaag aaacggcttg agatattccg
     7561 agcagttata aaaattaatt tgcgttgaat attgctacta cattcttgaa aaagtgatgt
     7621 agatttatag cccataactt ttttgaatta catcgacgat acggcgtcga aagaaaaagc
     7681 tacctcactt tatctggggg gcttttattt tttatgccct tattttgaat tttaagaggg
     7741 cattttaaag atttaggggt gattcatata gttttatatc taaaacttat atacgctttt
     7801 aaaacgcaaa tatgagccaa ataaatatat gctattttca aaaacaaaaa tttgagcaaa
     7861 aacaataact attttttaga cactgcccat ttacatgcaa attaaaaatt agcataaaat
     7921 tctttaaaac ttccccacgt tcttctaaat tcctgcaggg cttttcttat caaaaactta
     7981 gaaattttaa tttaactcta aaatctccgt aagagcataa gggaaatttg tccccatcaa
     8041 tgaatggggg atctggcaaa gccagatatt tcaaggcttc gaaaatgttt ttgtgtgggg
     8101 tcataaaaag ctcaagtgtc acactttgtg acactacata cagcatttgt cacaaaaata
     8161 agacagcact ttttttacaa aaaagtaata aaaaaagaca tcagttatca ctaatgcctt
     8221 aaataataat tttgcttttc aaaattgccg taatcgaatg tgccttttct tcttcatttg
     8281 ctattttatc ttcatcactt gtattaatta aattgccatc ttcagcatcg tctaagttta
     8341 aaattttatg tttttattta agcaatccac cataacttaa catacgtttt cgatacaaat
     8401 ctttttctag atcactgaca atttcttgat tttttcatcg ctatcagtta aaaagtcaga
     8461 tgatttaacg aaatattttg atgtatcttt gattgttata aaatcatttg aatgattgat
     8521 taaagatatt atattgtttc atgtttttac ggcgcatata aagtgcatgt gtttcttcaa
     8581 attcagttaa aattgtttgc attaaataac cttgcattga cttgaatgat tactaatttc
     8641 attttcggac tctatatcat tcaagtcatt catatcattt atttcagtat tgttagattc
     8701 agtatagcta agttcagtat cattagattc tgttttatcg acttcttgaa gtcggttttt
     8761 ccgacttcct tgccattgct gctctcaaca gtgttttctt gcttttgaat tttataaata
     8821 tcatcttctg ttacttctgg tttaactaaa taaagtttat ttgtcttatt taaaccttgt
     8881 ctttcttgta gtagtaattc ggcttcaatt aagttctttc tttattttaa ttactttatc
     8941 ttttttacaa tttaatatat tttgtgggtt ttcatttttg taataaaaat aaatattacc
     9001 tgttttagaa tcaacccaac catttttaat tgataaatct aaacgatctc taagaatacc
     9061 ccatgccatt tttgcatcat tggataactt gatatattta ggatttgtaa aaaatacttt
     9121 cggtatttga taaaactttt ctctgtattc ctcatttatg ttgtaaagtt taattgacat
     9181 aataaaaacc tctttccgaa ttttagtttg gtaagaggta ataaaacgct tacatttttg
     9241 tgtttattta ataatctagg tgcttgcact aagatattat tcattatata atctaagtat
     9301 cgaaaattat ataaataaac agtttctaaa cgttttatta ccaaaccccg actattacca
     9361 gtagttgggg tttttgttat attaaggtta ttatagcact ttgaataaac ttaaaaaggg
     9421 aaaatatagg tttataaacc tttgataaac accataaacc ctttacaaac ccttgataaa
     9481 aaggttggcg aatttatatt tttatttgtc gattacttta ttattaatcg ttataagctt
     9541 ataatatttt actaagggtt tataaacatt ttataaaggg ttgatgatat gaaaagtgta
     9601 aaaaggttga gtgaagaatt aggggttagt aagcaaacga tttttaataa tattaagaga
     9661 ttgaatatag agacaataaa acaggaaaac acttcgttta ttaaagaaga taccgatata
     9721 gaaaaaatca ttcaaagagt aaatgaaaat aaaaagaaat atggttttga aagtgctact
     9781 gaagacaaac aaaaaaagag tcggataata ttaattttga atctaaaagc gatgctcaaa
     9841 tagtagaaat acttaaaaat cagattaata ctttaaataa tcaaattgaa aaacaagaaa
     9901 gtcgtcatga aacaacaatt gaattttaca gaaaagagtt gcaagaaaga tcaaaattgc
     9961 ttgagaatca gcaagttctg gcattagaga gtaataaaaa gattcaaaaa ttagaaaatc
    10021 aattagaaaa agaaaaacac cttaattatc cctttgatac atctactaac gctagacaaa
    10081 acgttgatgc acaagaaaaa acatatacta ctagtcctgt aaatattaac cgagatcagg
    10141 aagaaactaa agaaacagag attcaatata aggatatatc tggaagtcaa agcgacgaaa
    10201 gtacacaagg tgaagaagca caaagagaag acgtttcagc taatccaaat gacaatgata
    10261 gtgatataga agaaaagagt gaagaaacgg aacctaaaag gggttttgga gtcgtctatt
    10321 tggtaactaa ttttaattaa aaaggtacga caagttcata aattagccgt gcttttttat
    10381 gtgaattgaa cttctgataa tcatcgatta tgttaaccaa acaaaaagcc ataaatgcct
    10441 atatgacggc atttatggct tttttacttt cattatcttt tatgcatctt ttatacatcg
    10501 ttgatttgac gcgttttcat tcttttgcat tggcaagtat tttgtatctt tattcagtta
    10561 ctatttcttt tctccaacct aacaaaccat ctttaactct aataatatgt ttgccacgcc
    10621 agacacttct tacttcgaaa taataccatt ccccgttttt gttgaggaaa taaatatctt
    10681 gataaccttc tttaatgaca tattttgtag cttcttgtct ttgatcgcca aacattatca
    10741 atcccaccct tatatttatt tatctttaat atataacaac atttattaat attcatgtta
    10801 tttcatggaa ggagagattt gttataatgt gaaggtcgtc tatctctcag atgtcaattc
    10861 ggcgtagaga ggaggtgcga tttatgaatg aaattcttgt tcacatcatg acaacggcaa
    10921 tcagtggttg tctcgttacg ttatttggtt attagctcca taaacgtgat aaaaaataat
    10981 atagacgatt aaagccaatt caccaaaaaa ccccgaacta cggcgaatag ttcggggttt
    11041 tgtgcgttta caaacgaaat tcttgttact tttagtatat atttcatatt gccttttgta
    11101 aactcttcta ttcttatcaa tacatttcaa ggtgtcgtaa aaatactttc ctttttcata
    11161 ttgcctgatg tgcattaaat tttgatttta aaatcaaccc atgaacgaat gaatggccca
    11221 aactaaaaat ccggcaacag gaaagaaaag ccaccaaaaa tcataaagaa aatcaactat
    11281 ggaaggtacc ctgttactct ctctataatt tccctctaaa ataacttttt taaaacgtgc
    11341 atctccttta attaaatgct ctaaagagat tccgaataaa tcgctcatag caatcaaaat
    11401 atctaaggaa ggatagtttt tggcactttc ccatttagaa acactttgtc tggaaacctt
    11461 taatatattg gctaattcct cttgagacca tccttttaat tctctctgtt cttttatagc
    11521 ttcagctaat ttcataaaat cactccttaa ctcaaactat aattaaaata attgaaaagt
    11581 cacgatactt ttagttaacc aactcgcaac gaaaagttgc gttattccca attcagttca
    11641 agttgtttta aaaaatcctc tcgttctttc tctaattgtg ggtcgtaatc tttttgaggt
    11701 tgcttttcat atgaccgttc ttctaaccat ttaggcgtct tttcacgcct cacatattgt
    11761 cttgatttat ctatcttgga catttgaggt tgtcgtttgt tatgaatgcg tttctcagcg
    11821 tcaaatgtga actctatcca ttcaatttta cgcccttttt tcgctttgat tttattgata
    11881 gtaagattat taaatataga acctaattca ataataatag gtttaaaaac atttttgtta
    11941 atatcagtca tacgataaga tttaggtata tctaaacgat ttttaaaatc atcgatgtga
    12001 atttttaaat agcctgtatg tttgtattgt ttaagtaatc taaacatatt tttagaatat
    12061 gtagatttaa gcctagtcat ttcttttaat tcaaattttg taaaattatt agtaattgaa
    12121 ttcaatatgt gttttaaatt tgaagaagtt gatatttcta gatattgttc tcgcttatgg
    12181 attctataat gattgaataa aacgaatctt tctattacat catcatcttc atatctaatg
    12241 gttaaattca acatcttttt atacacatta tctaaatcat tagcaaatct attaatatta
    12301 cgtgtttctg gactataatt acttaatttt tttaattcat caaaagacaa acgtaatgta
    12361 ttagtatctt gctcttttaa tttattacac atcgcaaaaa ataaattaat ttcagtagct
    12421 gtaaatcgcc ttaatggaac taaattcatt tcatttttat atacaactgt ctctcctgtc
    12481 atgatacagc cccctttatt acgaacctaa aggagtatcg tttgaatata cactcccatt
    12541 tgaaaacttt tattcacaat aaactacttt attatcaatg tcaaaccata aaaggagtac
    12601 aactttttaa aacactcctt ttatgtggca aaaacactcc ttttatgtgg caaaaacact
    12661 ccttttatgt ggcaaaaaca ctccttttat gtggcaaaaa cactccttta ggcattgaaa
    12721 tcccttgttg ctctagggct gaacaagccc ctaaaaggtt tttaaaaaga tttataaaaa
    12781 tatattaaaa agttcgaggg ggctacctat ttttcatcct aaaaaataat taacctcaaa
    12841 caaatgcatt taaatatcta tttttataat gtataaccat cttctctatc ttctttaaaa
    12901 atgatttctc gttcttgtgc ttgatatttc ttcttaaaga actgttcatc attgttatct
    12961 atcgtcatta cttcacgtat tttagaattg tctaaatgat tatcaatatg atttattagt
    13021 gtatgataac gctcttcttt catcatactt ttaatgattt taaaaacttg attgaataac
    13081 ttaagcgtat aatttagttc tttatctttc ttttcattct ctgacaacaa ataattataa
    13141 cgttgtgcta aatcattaaa ttcttcaacc agcttttcat tttccatatg gacactagtc
    13201 aattcattaa ctaaactttc attttgtttc tctgcttttt taatgacttc ccacttttaa
    13261 tacattcata atcatctaat atcatctgtg ctgctttaat ttgtttagtg tatttttcaa
    13321 aatcagtttt agtaattaca atattgcctg tttcttgctt ctctttatga aaccatccac
    13381 ctacattttt agttttaatt tcatacatga cactcagaag ctttttaagc gtatctaatg
    13441 atttttggta gttttgtatc aatttatcat tttcttgctt taaataatcc tctatttgct
    13501 tctagcgcat atgcatttgt ttatgatatt ctgttttttg tttataacgg ctaacttgat
    13561 catgtttttc ttttgtaagt tgtctagaaa ttcctcgttt taaatcatag ccttgcttat
    13621 taatatacgt attgaatcta tcttggaatt cggttaatgc ttttttatta cctacaactt
    13681 ctttcgcact taaacgtcca tctttagtaa taggaactac gccataatgc atatgtggtg
    13741 ttatctcatc catatgtact gtcgcatata aaagattttc tttaccgtat tcttcttcca
    13801 agaacgcttt agcatgctta aaaaactatt ttgtctcttc ttcagataat tgattaaaaa
    13861 acacattatc tgatgtaatt aatccatcaa tatgtttaat ggcatctgtt ctaattttgc
    13921 gtttcccttt ataatttttt tcgattttct catcaatttt tttattaaaa tcaaacttag
    13981 tatcattgac taaatcatag tttaaatatg acttacttaa atcaatatct atattttcat
    14041 aatttttatt ttctctttga atatgtcttt gaatacctct tgtatttgtt tttgatttca
    14101 ctttaacaac tcgaataatt gaataagaca taataaatca acttccactg catatgatta
    14161 taacacctaa acacagaaaa gttttaacat actagactta ttaaaaacca aataagtcat
    14221 taaaaacgtg tgctctgcga ggcttgtttt ttgtcatcat aaaaaaggac tggaaaatcc
    14281 agtccttaaa ttaaaaaact caaaagtaat gactaatcat atttcatatt gttttttctc
    14341 tatgtatggc tatacctatc aatatttctt aacttctaag ctcagaaata ttgactatga
    14401 catcaaatcg tttcgcaagc tctaaatcat gcgttacgac aatcatagtt ttattttcat
    14461 caagtagctt caataataaa tcaataacta tttttccatt tttcgtatct agtgacccag
    14521 taggttcatc agctaaaatg attttcggat cttttaatat taatcttgcc aatgctactc
    14581 tttgttgttc accaccgctt agagaactaa caattctctt gatatctaaa tctaggccga
    14641 ccttttttaa tacttctttc tttaagttca tattatcttt tttattcttt ttgaacttta
    14701 gggctaaatc taaattttga ctgattgttt gattttcaag taaagcaaaa ttttgaaaca
    14761 aataccctaa cgtatatcta taaaaatcga ctttcttttt tagtaactta tcttcaatat
    14821 atatgcttcc ttgtgataca tcttcgaacc cagcgattat gttaagtaaa gtagattttc
    14881 cagcgccact ttctccaact aaagcatatg attttccttt ttcaaaatta aagttacatc
    14941 cgtctaaaat gtttttatta ccttttttta caaaaacatt ttcaagtttt atcactatcg
    15001 gtcacctcat ttaaaaagct aggaatatct tttgtaatgt attctatcga acgcacatat
    15061 ttccctttat gatttacttt cacatattct tttgtaggat cataacctaa aaaatccaat
    15121 ttataactct tttttctcca tttttagagt atacagttat atttctatag tcctgggtat
    15181 cttttggaac tttggcataa gatacttttt cttttaacag tgggttaaaa cgatctaaat
    15241 gctcatttct aaccaataac agactaagta tgatgattaa tagtacaatt gttacaataa
    15301 ttaaagttct tttcattgtt taaatctccc tcattaaatc tttaaagtat ttttcaagat
    15361 atttaatata agcaatttga agtaaatgtt gaataatagc aatagacatt attatgaata
    15421 gtaatatctt tgaaccaaat aaaatatgcg tagtgtaagc taacataata gaaagtaaga
    15481 tagaaattgt taaataacgt acattactgc tgaataatga ataaccatga atttttctta
    15541 ataatagttg ctttttattc cagctaaaat attgaattgt ttcaaataca gtagctatca
    15601 aaagaatgaa aacgttaata ataatagtaa ctattgttat tatcattttt gttttcgttt
    15661 catttaattc atttaacaca gaatccttgt aattagttat accgctaatt tcttcttcta
    15721 ggtgattatc tttaagcaac tgtttaagtg tatctaaatc tttaaataga tatccacctt
    15781 gagaaacagc agctaaataa aaatctcctg ttaaatcgtc aagtgtcaac aacattacta
    15841 ttggagaatc tagataccca aatttcaaat tagttgtgcg atcaaaagaa aaacagatac
    15901 gttttttgac agtacttgca catctacttt ttgttttgtt gcttttgaaa gtcaatccat
    15961 ccttggtgat ttttttgaat ttcagatttt tgatattgta accttggagg aataaacacg
    16021 tttattttat ccgaatgatt gtcaatcatt ttaaaatcat gatttatatt tttatagaaa
    16081 ttcagaaaat tgttattgat aaaaaagaca ttgccatttt caggaatgta attgttttta
    16141 tcgggattgg ctatattatt attctttaaa agcaatccac caagttgttc gcttttctta
    16201 attaaattgt atatattagt ttctaaaatt tgtttttctt ctgaatttct aatattagga
    16261 gaaatctcta atgtatagta attatctaac ttttgccaga ttttttcaga gtcatatata
    16321 ttctttaact tcgtataagc atttaaattt tgtgccagta aaaaagatag tattagcacc
    16381 atgatacatt tggtgaaggt acccataaat attgaaaact tatatccttt tttccctttt
    16441 aagattaatg gaatacttaa cttcaaaaga agactatacg aaatcagcca cagcaatgta
    16501 gtaatacaag caaaaacaaa aagcaacacg ataaatctac gaataaacat taagaaatcc
    16561 aattctaaat tggctatatg aatgattaat atattagcaa taaagaaaat agcggtaagt
    16621 actagccaat aaatgatttt tatatgaata ttatgaaaaa taatgcgtgt agttgaaaag
    16681 ccatgtagct tttgaatagc ataaaatttc aaattatatc ctctgtcata taagaagtac
    16741 aaaagatata atacgcctaa gaatatgaaa ggcactaata aatcattatc tatgactaat
    16801 tcaaaaaata atatgtttct atttatgtat tcaacttcag atgttaaacc agcttttttt
    16861 aattcactcg taagctcttc tacattgaaa tgttcaccaa taatataata ctttcctttt
    16921 atctctctgt ttaataaatc tgatggagtt aaaagtgatg ttttattacg tataaagtca
    16981 gaattatttc gttttgagtt gaaaacaaaa attctttttt attatcaaaa tctttaacgg
    17041 atttaattat ttgtatattt tgatttttgg cgcctgattc aatgacatct aacacttttg
    17101 atttactgtt agatttatcc caattagaga ttgttaaaat attggaactt tcaggtataa
    17161 tttcttcatt ttctttgaag aaaaataaaa ataatagccc tgaaattaat aataaagtac
    17221 taaaatctaa aaataatttg aattttttca tattgcctct ttcaagagaa tagacagaat
    17281 taagtttact taattctgtc tattctcctt aattaatata tgtaattagt gaagactata
    17341 aaatgttttg tttccacccc aagcctttgg agcagatgcc ttagattgaa caccaggtcg
    17401 agcaacacca ctaaaagatg aatatttccc tactgctgtt gaaccatgat ttcttttatt
    17461 gtgactataa taagaccata cgtaatgttt tccaatacca tggctccaaa caccaccagc
    17521 tacatgtact gttgcagctt cagctttata agttacacct aataaagtgc cgaataagat
    17581 tgtactagca atacaactag aaacaaattt ctttttcata aaaacacctc catttaatat
    17641 tctttattta ttatagcaaa aataaataaa atagagaatg agtaaaaata atctaaaagt
    17701 gttttttctg ctctgttgct tgttttgttc atctatatat aatattaatt ctattatcct
    17761 taaattaact atcattaaac aacgatatga caatgtataa taaatacaat ttcactacct
    17821 ataattcaac attatataaa ctaaagtatt tcaaataatt aactgtatta atactctcat
    17881 catgattgcg ttataaacat tcgttaatga gaaaaagaga gagcatattt acgctctctt
    17941 ttcttgtctt attattaaaa actcataata gattataatc atctgttttg agacgctgtg
    18001 agaagctctc agttgcattg aaacaaagtt tattaaatca aatcattatc gtttttaatt
    18061 ctgtaaatcg tctgccttgt tatatttact tctttcgcaa ttttactaat tgattggcct
    18121 tcttctaaca tttctacaac gcgatgataa ataatacgtt tctgtggatc tttagcattt
    18181 ggtgaataaa gtaaggggcg ccctttatat acgccttttt ccttcgcaac ttgaattcct
    18241 tgcgcttgtc gacgtttact ttcatttctc tcttgttctg aaaccattgc taatatttga
    18301 ataattaaat ctttcataaa cttatccagt aatggattgc caaccacttc attcatcata
    18361 ggtaagctag taatcattaa ttgtacttct ttgtctttta aataattaac ggtatgaatc
    18421 acttcatcat aattacgacc taaacaatca atggattcta ctacaaaccg atctcccatc
    18481 ctcaaaaagt taagtgcttc ttgaagtact ggtctatttt caatagattt cccagactgt
    18541 ttttctgtaa atattttttc agcacaaaac gtctttaaat tttcaagttg tctttctaaa
    18601 ttttgatcgt tcgatgatac tctcgcatag ccaacaatca ttgatttcca tctcataatt
    18661 aatatacacc ctaatcatac gtttcagtta ctataaattc aacaattatg tcactttgct
    18721 tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga
    18781 tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat
    18841 tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa
    18901 taataaaggg aaaatcaggt tttgatatca aaattataca tgtcaacgat aatagaaaat
    18961 ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaatat taaaattcct
    19021 tcattacact cttggcggtt tcacttatca acttatcatt tggcttatca cttttattgt
    19081 ctttattcgt aaaaatgact aaaacaatag gttcagattg gcccttagga taaacaaaag
    19141 caacatcatt tctagaagca tatgttattg cttgaccact tttatcagca accttatagt
    19201 cttttggaac accatcttta attaaagtgt ctccgttttt attattaaac attaaatcaa
    19261 gtaagaaatt tttatttttt tgcttaattt tccatttgcg ataagtttat ttaaagtctt
    19321 gccgaaagca gcaggcgttg aagtatcttt ttgctctttg gcgaatagta atttaattct
    19381 atctcatatc taactggatt tgttacttta tctcctagtt cttttagacg ttgtttaact
    19441 tttttgattc caccgatttc ttttataatt ttattgtttg ctgtattatc actatatgtc
    19501 attgaagcct caataagttc ttttaaagtg atgtcttttc ctacatattt ttctaaaata
    19561 ggagaataag caactatatc atctttgtta atatgtactt ttttatttaa cttattataa
    19621 ggtacttgtt ctaacaaaat agcactattt atcgcttttg aagtcgaagc ataggcaaat
    19681 ctcttatctg aattaaattt tacttcctta ccacttttag tatctaaagc ataaacacca
    19741 atatgagcat tatatttttt tctaaatcat ttaactcttt ggcatgtgaa ctgtttgaat
    19801 tacatgcact taaaactaaa gccattacaa ttaaaaatat taactttttc aaaataaacc
    19861 ctccgatatt acagttgtaa tttttacaaa acaataatat tacaattgta atattgatgt
    19921 caattgttta ttataaatat ttgaaagaag gtgtcaaaat ggctaaatta ctaataatga
    19981 gtgtagtaag cttttgtttt atatttctat tgttagtatt ttttaggtac atattaaaac
    20041 gctattttaa ttatagttta aattataaag tttggtatct aactgtgctt gctggattaa
    20101 ttccttttat cccaattaaa ttctctttta taaaatttaa taatgtgaat aatcaagcgc
    20161 ccacagttga aagtaagtca cacgacttga accataacat aattaccacc aaacctgttc
    20221 aagagttcgc aacagatatc cataagttta attgggattc aattgataat atctgcacag
    20281 ttatttggat agttttagtt attattttaa gttttaaatt tttgaaagcc ttattatatc
    20341 ttaaatattt aaagaaacag tcactttatc taaacgaaaa tgaaaaaaat aaaatagata
    20401 cgatactttt caaccatcaa tataaaaaaa tattgtgatt cgaaaagcag aggctattca
    20461 atctccaata actttttggt atgggaaata tattattttg attcctagtt catattttaa
    20521 aagtgtaatt gataaaagac taaaatatat aatactgcat gaatatgctc atgctaaaaa
    20581 tagagatact ttacatttaa ttatctttaa tatcttctct attattatga gttacaatcc
    20641 attagtacat attgtaaaaa gaaagataat tcatgacaat gaagtagaag ccgatagatt
    20701 tgtccttaat aatattaaca aaaatgaatt taaaa
//
