LOCUS       CP127704                 768 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C133 plasmid pC133_2, complete
            sequence.
ACCESSION   CP127704
VERSION     CP127704.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360872
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 768)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C133
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 23x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 03:46:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,795
            CDSs (total)                      :: 2,713
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 37 of 56
            Pseudo Genes (incomplete)         :: 26 of 56
            Pseudo Genes (internal stop)      :: 12 of 56
            Pseudo Genes (multiple problems)  :: 16 of 56
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C133"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC133_2"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2012-11-12"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 73"
ORIGIN
        1 tggttgtttt gtgatctctt ctgttggttg accctcacca actttttcac ctgttaatgg
       61 gttcactgtg attggtgttg tgattgtctt acttcctggt tgtccctctt gtttcactcg
      121 ctcttcacca ggttgtaact tcggattaaa ttcacgtttt gtttcaaacg gtatctctac
      181 tgtttttgtt tctggtgtgc ctgtttttgg tccgtgttta atcacatcat ccactggctc
      241 ttcgatcact tttcctgtgt ctggattctt gattcctggt ttacctggta ctttttccgt
      301 ttgatctgtt ggtaagtttg gatcaaagat atctttatga ccttgcggta ttttctcgcc
      361 accaaactct acaatcttat ctattggttg ttttgtgatc tcttctgttg gttgaccttc
      421 gccaactttt tcacccgtta atgggttcac tgtgattggt gttgtgattg tcttacttcc
      481 tggttgtcct tcttgtttca ctcgctcttc accaggttgt aacttcggat taaattcacg
      541 ttttgtttca aacggtatct ctactgtttt tgtttctggt gtacccgttt ttggtccgtg
      601 tttaatcaca tcatccactg gctcttcgat cacttttcct gtgtctggat tcttgattcc
      661 tggtttacct ggtacttttt ccgtttgatc tgttggtaag tttggatcaa agatatcttt
      721 atgaccttgc ggtattttct cgccaccaaa ctctacaatc ttatctat
//
