LOCUS       CP127644                2397 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C214 plasmid pC214_2, complete
            sequence.
ACCESSION   CP127644
VERSION     CP127644.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360908
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C214
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 75x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 02:04:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,759
            CDSs (total)                      :: 2,677
            Genes (coding)                    :: 2,607
            CDSs (with protein)               :: 2,607
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 32 of 70
            Pseudo Genes (incomplete)         :: 36 of 70
            Pseudo Genes (internal stop)      :: 16 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C214"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC214_2"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2013-12-18"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 109"
     gene            join(2190..2397,1..236)
                     /locus_tag="PCK73_13785"
     CDS             join(2190..2397,1..236)
                     /locus_tag="PCK73_13785"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJA77245.1"
                     /translation="MYKKILTLSILLVMIFTVVPVHNEVEAKPKKGSVVTINKKQYIYQ
                     GTVKKYYPAKYCKLVNKYAKNSQSFTNLVAGTSGFATFAKKAGYAGVLASSLYIANLGV
                     QNNTTIYKNAARKGTGVQVSYDLYVPKTGHNYGLTRNTKVVYK"
                     /name="hypothetical protein"
     gene            536..1462
                     /locus_tag="PCK73_13790"
     CDS             536..1462
                     /locus_tag="PCK73_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WJA77246.1"
                     /translation="MERYESKKVRSEEFRDLVKSLVSDSYYWLIEECGTFLSFVGDKEL
                     NKIKMHRGNFCKHRFCPMCAWRKARKDALELSLMMQDIKISYDKAFIFLTLTTPNVVAE
                     RLGDEIKRYNKSFDKLFKRKVVKRVVKGYVRKLEITYNEKRDDYHPHFHVLIVVNKSYF
                     TDVKAYLNQKEWLRLWREATGISEITQVDVRRVKEQNSNELYEMAKYTGKDSDYLSNEK
                     VFKVFYESLKGKRAFAYGGLFKEVRKKLKNGDLDYLKEVDPTKYIYQIFCGWNQMHYYA
                     SELRLMSEEMQKEVNHQMINELDDKYL"
                     /name="protein rep"
     gene            1958..2140
                     /locus_tag="PCK73_13795"
     CDS             1958..2140
                     /locus_tag="PCK73_13795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WJA77244.1"
                     /translation="MKYRIIAALFFLMLLLIYVFKIAPFLNTDSQIVNLVVVLIIFFIG
                     ALLGWISRKFDKNSK"
                     /name="hypothetical protein"
     Typing          498..1462
                     /name="rep_cluster_1130"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttacaaattt agtggctggt actagcggtt ttgcgacttt tgcaaaaaaa gctggttatg
       61 ctggtgtgtt agcttcgagt ttatatattg ctaatttagg tgttcagaat aatactacga
      121 tttacaaaaa tgctgcaaga aaaggtacgg gtgtgcaagt tagttatgat ttatatgttc
      181 ctaaaacagg acataattat ggattaacac gtaatacaaa agttgtatat aaataaaaaa
      241 atggagatgt ttttcatctc cattttttta ttttcttcta tcttgataat ataggtaaca
      301 actcacggat gtctgtatag gcatctaacc cttgataata aaggaaaaaa catagaaaaa
      361 cttgctttat ccagcatatt attctagaat tttaggtgtc taatcaaaaa ttctaaatat
      421 tggaggtata aagcaagttt tttgttcgta atatttttgt atccaaattt atagcgtctt
      481 aaaatttgtt aattacatga cacataataa tataaacaat cgtaaaatac aagatattga
      541 gagatatgag agtaaaaagg ttagaagtga agagtttaga gatttagtaa aaagtttagt
      601 atctgattcg tattattggt taattgagga atgtgggaca ttcttatcgt ttgttggtga
      661 taaagaatta aacaaaatta aaatgcatcg tgggaatttt tgtaagcata gattttgtcc
      721 aatgtgtgca tggagaaaag cgagaaaaga tgctttggaa ttatctttaa tgatgcagga
      781 tataaagatt agttatgata aagcatttat ctttttgacg cttacaacac cgaacgtagt
      841 agctgaaaga ttgggagatg agattaaaag atataataaa tcttttgata aattatttaa
      901 acgaaaagtt gtaaaaagag tagtaaaagg ttatgttcga aaattagaaa taacttataa
      961 tgagaaacga gatgattatc acccacattt tcatgtgctt attgttgtta ataaatcata
     1021 ttttacggat gtaaaagctt atttgaatca aaaagagtgg ttgcgtttat ggcgtgaggc
     1081 aacaggaatt tcagaaatta cgcaagtaga tgtgcgtcgg gtcaaagagc aaaatagtaa
     1141 tgagttgtat gaaatggcta aatatacagg taaagatagt gattatttat ctaatgaaaa
     1201 agtgtttaaa gttttttatg agtctttaaa aggtaaaagg gcatttgcat atggtggatt
     1261 atttaaagaa gttagaaaaa agcttaaaaa tggcgattta gattatttaa aagaggttga
     1321 tccaacaaaa tacatatatc aaattttttg tggttggaat caaatgcatt attatgcgag
     1381 tgaattgaga ttgatgagtg aagaaatgca aaaagaagtg aatcatcaaa tgataaatga
     1441 attagatgat aaatatttat aagagcttta tttttcaatt ttatatataa agaattgcat
     1501 atgtaattta gtgtagtctg aggtgctgac agagtcagca aggcggagcc gtccagcgga
     1561 gcgttcagac aagtaatata aaatgtgtag gcggagtttt agaattaagt aaaagaattt
     1621 gtttttgaat gtatttaaag ccattaaaaa gtattaaact aattttggac gtataaatgt
     1681 atcgattagt tattttgttt taaaatatgc ttaaaatgcg aaataatcat atattcacat
     1741 ttatgccgag aaaacatttt gaaaaagaaa aaagctatat gatatttcaa cttgttgaaa
     1801 atgttggtgg ggtttgaatt gggattgatt ccaagaaaac accaacagaa aatcatatag
     1861 cttaaagttt ctgttcaaaa tggaaattgg aataaattta tggtttattg tataagaatg
     1921 aatatagaat ttattaagaa agattaggag gatttatatg aaatatagaa ttatagcggc
     1981 tttgtttttt ttgatgttgt tattaatata tgtatttaaa atagctcctt ttttaaatac
     2041 tgatagtcaa attgtaaatt tagttgttgt gttaattatt ttctttatag gtgcattatt
     2101 agggtggatt agtcgtaaat ttgataaaaa ctccaaataa atatattttt gtgttatatt
     2161 aatacaaaaa tatattaggg gtgtatttta tgtataaaaa aatattaact ttatctattt
     2221 tgcttgttat gatttttaca gttgttcctg ttcataatga agttgaagct aaacctaaaa
     2281 aaggttctgt tgtaacgatt aataaaaagc aatatatata tcaagggact gtaaaaaaat
     2341 attatcctgc aaaatattgt aaactggtta ataagtatgc taagaattcc cagtctt
//
