LOCUS       CP127641                4439 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C236 plasmid pC236_1, complete
            sequence.
ACCESSION   CP127641
VERSION     CP127641.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360910
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C236
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 54x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 01:54:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,845
            CDSs (total)                      :: 2,763
            Genes (coding)                    :: 2,652
            CDSs (with protein)               :: 2,652
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 111
            CDSs (without protein)            :: 111
            Pseudo Genes (ambiguous residues) :: 0 of 111
            Pseudo Genes (frameshifted)       :: 45 of 111
            Pseudo Genes (incomplete)         :: 49 of 111
            Pseudo Genes (internal stop)      :: 42 of 111
            Pseudo Genes (multiple problems)  :: 22 of 111
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C236"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC236_1"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2014-05-26"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 111"
     ARG             2..495
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="35.73"
     ARG             3556..4439
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="99.66"
                     /coverage="64.27"
     gene            681..1922
                     /gene="mobV"
                     /locus_tag="PCM75_14220"
     CDS             681..1922
                     /gene="mobV"
                     /locus_tag="PCM75_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WJA50998.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            2449..3393
                     /locus_tag="PCM75_14225"
     CDS             2449..3393
                     /locus_tag="PCM75_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WJA50996.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          2450..3393
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          682..1919
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatagtgtta tttttcctgg aaccatgagt gttattgttt ttggttattt tggtggtttt
       61 ttagtggata gaaaaggatc attatttgtt tttattttag gatcattgtc tatctctata
      121 agttttttaa ctattgcatt ttttgttgag tttagtatgt ggttgactac ttttatgttt
      181 atatttgtta tgggcggatt atcttttact aaaacagtta tatcaaaaat agtatcaagt
      241 agtctttctg aagaagaagt tgcttctgga atgagtttgc taaatttcac aagtttttta
      301 tcagagggaa caggtatagc aattgtagga ggtttattgt cactacaatt gattaatcgt
      361 aaactagttc tggaatttat aaattattct tctggagtgt atagtaatat tcttgtagcc
      421 atggctatcc ttattatttt atgttgtctt ttgacgatta ttgtatttaa acgttctgaa
      481 aagcagtttg aatagttata ttatattttg gtttagaact atgagtggct agcattttgc
      541 cactcatttt ttgcgttagc aaaaacaggt ttaagcctcg cagagcacac gtattaacga
      601 cttattaaaa ataagtctag tatgttagac ttaaactatt aaatacacat gaaacctttg
      661 tgcttaggag tgatttttat atgtcttatt ccattgttag agtttcaaaa gttaaatctg
      721 gaacaaatac aacgggcata caaaaacatg ttcaaagaga aaataataat tatgaaaatg
      781 aagatataga ccatagtaaa acttacttaa attatgattt ggtaaatgct aataaacaga
      841 attttaataa cttgattgat gaaaaaatcg aacagaatta tacaggcaaa agaaaaatta
      901 gaacagacgc gattaaacac attgatggtt taattacatc agacaatgat ttctttgata
      961 atcaaacgcc agaagataca aagcagtttt ttgaatatgc taaagagttt ttagaacaag
     1021 aatacggtaa agataattta ttatatgcaa cagttcacat ggacgaaaaa acaccacata
     1081 tgcattatgg cgttgttcca ataactgatg atggtcgttt aagtgctaaa gaagttgtag
     1141 gtaataaaaa agctttaaca gcgtttcaag atagatttaa tgagcatgtt aaacaacgag
     1201 gatatgattt agaacgtggg caatcaagac aagtaacaaa tgctaaacat gagcaaataa
     1261 gtcagtataa acaaaaaaca gaatatcata agcaagaata tgaacgtgag agccaaaaaa
     1321 cagaccatat aaagcaaaag aacgataaat taatgcaaga gtaccaaaaa tcgttaaata
     1381 cgcttaaaaa gcctataaat gttccgtatg agcaagaaac tgaaaaagta ggtggtttat
     1441 ttagcaaaga aatacaagaa actggaaatg ttgtaataag ccaaaaagat ttcaatgaat
     1501 ttcagaaaca gataaaagct gctcaagata tttcggaaga ttacgagtat ataaagtctg
     1561 gtagagcctt agatgataaa gataaggaaa tacgagagaa agatgattta ttaaataaag
     1621 cagttgagcg tattgaaaac gcagacgata attttaacca actttacgaa aatgcaaagc
     1681 cacttaaaga gaatatagaa atagcgttaa agcttttaaa aatcttacta aaagagttag
     1741 aacgagtttt aggaagaaat acctttgcgg aaagagttaa taagttaaca gaagatgaac
     1801 caaaactaaa tggtttagca ggaaacttag ataaaaaaat gaatccagaa ttatattcag
     1861 aacaggaaca gcaacaagaa caacaaaaga atcaaaaacg agatagaggt atgcacttat
     1921 agaacatgca tttatgccga gaaaacttat tggttggaat gggctatgtg ttagctaact
     1981 tgttagcgag ttggttggac ttgaattggg attaatccca agaaagtacc aactcaacaa
     2041 cacataaagc cctgtaggtt ccgaccaata aggaaattgg aataaagcaa taaaaggagt
     2101 tgaagaaatg aaattcagag aagcctttga gaattttata acaagtaagt atgtacttgg
     2161 tgttttagta gttttaactg tttaccagat aatacaaatg cttaaataaa aaaagacttg
     2221 atctgattag accaagtctt ttgatagtgt tatattaata acaaaataaa aaggagtcgc
     2281 tcacgccctg accaaagttt gtgaacgaca tcattcaaag aaaaaaacac tgagttattt
     2341 ttataatctt gtatatttag atattaaacg atatttaaat atacatcaag atatatattt
     2401 gggtgagcga ttccttaaac gaaattgaga ttaaggagtc gattttttat gtataaaaac
     2461 aatcatgcaa atcattcaaa tcatttggaa aatcacgatt tagacaattt ttctaaaacc
     2521 ggctactcta atagccggtt ggacgcacat actgtgtgca tatctgatcc aaaattaagt
     2581 tttgatgcaa tgacgatcgt tggaaatctc aaccgagaca acgctcaagc cctttctaaa
     2641 tttatgagtg tagagcccca aataagactt tgggatattc ttcaaacaaa gtttaaagct
     2701 aaagcacttc aagaaaaagt ttatattgaa tatgacaaag tgaaagcaga tagttgggat
     2761 agacgtaata tgcgtattga atttaatcca aacaaactta cacgagatga aatgatttgg
     2821 ttaaaacaaa atataataag ctacatggaa gatgacggtt ttacaagatt agatttagcc
     2881 tttgattttg aagatgattt gagtgactac tatgcaatgt ctgataaagc agttaagaaa
     2941 actatttttt atggtcgtaa tggtaagcca gaaacaaaat attttggcgt gagagatagt
     3001 aatagattta ttagaattta taataaaaag caagaacgta aagataatgc agatgctgaa
     3061 gttatgtctg aacatttatg gcgtgtagaa atcgaactta aaagagatat ggtggattac
     3121 tggaatgatt gctttagtga tttacatatc ttgcaaccag attggaaaac tatccaacgc
     3181 actgcggata gagcaatagt ttttatgtta ttgagtgatg aagaagaatg gggaaagctt
     3241 cacagaaatt ctagaacaaa atataagaat ttgataaaag aaatttcgcc agtcgattta
     3301 acggacttaa tgaaatcgac tttaaaagcg aacgaaaaac aattgcaaaa acaaatcgat
     3361 ttttggcaac atgaatttaa attttggaaa tagtgtacat attaatatta ctgaacaaaa
     3421 atgatatatt taaactattc taatttagga ggattttttt atgaagtgtc tatttaaaaa
     3481 tttggggaat ttatatgagg tgaaagaata atttacccct ataaacttta gtcacctcaa
     3541 gtaaagaggt aaaattgttt agtttatata aaaaatttaa aggtttgttt tatagcgttt
     3601 tattttggct ttgtattctt tcatttttta gtgtattaaa tgaaatggtt ttaaatgttt
     3661 ctttacctga tattgcaaat cattttaata ctactcctgg aattacaaac tgggtaaaca
     3721 ctgcatatat gttaactttt tcgataggaa cagcagtata tggaaaatta tctgattata
     3781 taaatataaa aaaattgtta attattggta ttagtttgag ctgtcttggt tcattgattg
     3841 cttttattgg tcacaatcac ttttttattt tgatttttgg taggttagta caaggagtag
     3901 gatctgctgc attcccttca ctgattatgg tggttgtagc tagaaatatt acaagaaaaa
     3961 aacaaggcaa agcctttggt tttataggat caattgtagc tttaggtgaa gggttaggtc
     4021 cttcaatagg gggaataata gcacattata ttcattggtc ttacctactt atacttccta
     4081 tgattacaat agtaactata ccttttctta ttaaagtaat ggtacctggt aaatcaacaa
     4141 aaaatacatt agatatcgta ggtatttttt taatgtctat aagtattata tgttttatgt
     4201 tatttacgac aaattataat tggacttttt taatactctt cacaatcttt tttgtgattt
     4261 ttattaaaca tatttcaaga gtttctaacc cttttattaa tcctaaacta gggaaaaaca
     4321 ttccgtttat gcttggtttg ttttctggtg ggctaatatt ttctatagta gctggtttta
     4381 tatcaatggt gccttatatg atgaaaacta tttatcatgt aaatgtagcg acaataggt
//
