LOCUS       CP127589                2473 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C868 plasmid pC868_1, complete
            sequence.
ACCESSION   CP127589
VERSION     CP127589.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360937
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C868
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 66x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 01:50:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,778
            Genes (coding)                    :: 2,692
            CDSs (with protein)               :: 2,692
            Genes (RNA)                       :: 78
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 45 of 86
            Pseudo Genes (internal stop)      :: 25 of 86
            Pseudo Genes (multiple problems)  :: 20 of 86
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C868"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC868_1"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2019-11-22"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 138"
     gene            195..671
                     /locus_tag="PCM57_14270"
     CDS             195..671
                     /locus_tag="PCM57_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WIZ79920.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            1366..1425
                     /gene="ermCL"
                     /locus_tag="PCM57_14275"
     CDS             1366..1425
                     /gene="ermCL"
                     /locus_tag="PCM57_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WIZ79921.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     gene            1486..2220
                     /gene="erm(C)"
                     /locus_tag="PCM57_14280"
     ARG             1487..2217
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1487..2220
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          196..681
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccgacgctt tatatagaaa agaagattca actaggtaaa atcttaatat aggttgagat
       61 gataaggttt ataaggaatt tgtttgttct aatttttcac tcattttgtt ctaatttctt
      121 ttaacaaatg ttcttttttt tttagaacag ttatgatata gttagaatag tttaaaataa
      181 ggagtgagaa aaagatgaaa gaaagatatg gaacagtcta taaaggctct cagaggctca
      241 tagacgaaga aagtggagaa gtcatagagg tagacaagtt ataccgtaaa caaacgtctg
      301 gtaacttcgt aaaggcatat atagtgcaat taataagtat gttagatatg attggcggaa
      361 aaaaacttaa aatcgttaac tatatcctag ataatgtcca cttaagtaac aatacaatga
      421 tagctacaac aagagaaata gcaaaagcta caggaacaag tctacaaaca gtaataacaa
      481 cacttaaaat cttagaagaa ggaaatatta taaaaagaaa aactggagta ttaatgttaa
      541 accctgaact actaatgaga ggcgacgacc aaaaacaaaa atacctctta ctcgaatttg
      601 ggaactttga gcaagaggca aatgaaaaac aagaaaatgc attatctgat tattattctt
      661 tcaaggacta gtataacata aaatcgtcta caaatagaca aaaaacctgc acgcttaatg
      721 tagatcaaaa gcttaacgca aatgaaatag attgacctcc caataacacc acgtagttat
      781 tgggaggtca atctatgaaa tgcgattaag ctttttctaa ttcacataag cgtgcaggtt
      841 taaagtacat aaaaaatata atgaaaaaaa gcatcattat actaacgtta taccaacatt
      901 atactctcat tatactaatt gcttattcca atttcctatt ggttggaacc aacaggcgtt
      961 agtgtgttgt tgagttggta ctttcatggg attaatccca tgaaaccccc aaccaactcg
     1021 ccaaagcttt ggctaacaca cacgccattc caaccaatag ttttctcggc ataaagccat
     1081 gctctgacgc ttaaatgcac taatgcctta aaaaaacatt aaagtctaac acactagact
     1141 tatttacttc gtaattaagt cgttaaaccg tgtgctctac gaccaaaagt ataaaacctt
     1201 taagaacttt cttttttctt gtaaaaaaag aaactagata aatctctcat atcttttatt
     1261 caataatcgc atcagattgc agtataaatt taacgatcac tcatcatgtt catatttatc
     1321 agagctcgtg ctataattat actaatttta taaggaggaa aaaatatggg catttttagt
     1381 atttttgtaa tcagcacagt tcattatcaa ccaaacaaaa aataagtggt tataatgaat
     1441 cgttaataag caaaattcat tataaccaaa ttaaagaggg ttataatgaa cgagaaaaat
     1501 ataaaacaca gtcaaaactt tattacttca aaacataata tagataaaat aatgacaaat
     1561 ataagattaa atgaacatga taatatcttt gaaatcggct caggaaaagg gcattttacc
     1621 cttgaattag tacagaggtg taatttcgta actgccattg aaatagacca taaattatgc
     1681 aaaactacag aaaataaact tgttgatcac gataatttcc aagttttaaa caaggatata
     1741 ttgcagttta aatttcctaa aaaccaatcc tataaaatat ttggtaatat accttataac
     1801 ataagtacgg atataatacg caaaattgtt tttgatagta tagctgatga gatttattta
     1861 atcgtggaat acgggtttgc taaaagatta ttaaatacaa aacgctcatt ggcattattt
     1921 ttaatggcag aagttgatat ttctatatta agtatggttc caagagaata ttttcatcct
     1981 aaacctaaag tgaatagctc acttatcaga ttaaatagaa aaaaatcaag aatatcacac
     2041 aaagataaac agaagtataa ttatttcgtt atgaaatggg ttaacaaaga atacaagaaa
     2101 atatttacaa aaaatcaatt taacaattcc ttaaaacatg caggaattga cgatttaaac
     2161 aatattagct ttgaacaatt cttatctctt ttcaatagct ataaattatt taataagtaa
     2221 gttaagggat gcataaactg catcccttaa cttgtttttc gtgtacctat tttttgtgaa
     2281 tcgattatgt cttttgcgca ttcacttctt ttctatataa atatgagcga agcgaataag
     2341 cgtcggaaaa gcagcaaaaa gtttcctttt tgctgttgga gcatgggggt tcagggggtg
     2401 cagtatctga cgtcaatgcc gagcgaaagc gagccgaagg gtagcattta cgttagataa
     2461 ccccctgata tgc
//
