LOCUS       CP127586                2598 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C870 plasmid pC870_2, complete
            sequence.
ACCESSION   CP127586
VERSION     CP127586.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360939
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C870
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 01:47:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,850
            CDSs (total)                      :: 2,768
            Genes (coding)                    :: 2,694
            CDSs (with protein)               :: 2,694
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 74
            CDSs (without protein)            :: 74
            Pseudo Genes (ambiguous residues) :: 0 of 74
            Pseudo Genes (frameshifted)       :: 44 of 74
            Pseudo Genes (incomplete)         :: 32 of 74
            Pseudo Genes (internal stop)      :: 24 of 74
            Pseudo Genes (multiple problems)  :: 25 of 74
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C870"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC870_2"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2020-05-27"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 140"
     gene            complement(join(2585..2598,1..310))
                     /gene="qacG"
                     /locus_tag="PCM83_14245"
     ARG             complement(3..310)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacG"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="96.26"
     gene            631..1554
                     /locus_tag="PCM83_14250"
     CDS             631..1554
                     /locus_tag="PCM83_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WIZ69155.1"
                     /translation="MEKYTEKKQRNQVFQKFIKRHIGENQMNLVEDCNTFLSFVADKTL
                     EKQKLYKANSCKNRFCPVCAWRKARKDALGLSLMMQYIKQQQKKEFIFLTLTTPNVMSD
                     ELENEIKRYNNSFRKLIKRKKVDSVIKGYVRKLEITYNKKRDDYNPHFHVLIAVNKSYF
                     TDKRYYISQQEWLDLWRDVTGISEITQVQVQKIRQNNNKELYEMAKYSGKDSDYLINQK
                     VFDAFYKSLKGKQVLVYSGLFKEAKKKLKNGDLDYLKEIDPTEYIYQIFYIWKQKEYLA
                     SELYDLTEQEKREINHKMIDEIEEEQ"
                     /name="protein rep"
     Typing          620..1554
                     /name="rep_cluster_1230"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.65"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaatacatt taagagcact acacctataa caattaagcc aatagaaatt atactaatta
       61 aattaacatt ttctttaaaa acgataactg agattattgt tgttaatact aatcctagac
      121 ctgcccatgt tgcgtaagtt atatttaagg gcaaaaattt tattgttaaa cttaaaaaat
      181 aaaagcaaat tccaaacgaa agtagtgtac ctaatgttgg ccataacttt gtgaaacctt
      241 ctgatgtttt taaaaaacta gttccgatta tttctgttgc aattgagata aataaatata
      301 aataatgcat taagcttctc ctttaatttc tatttgatag cttatatttt aatatatatt
      361 aagtgaaaga aaaattttta agatttgtat tttaaaacga gttgaaaacg agtttcttct
      421 tgtcttgata ataagggtaa ctattgccgg cgaggctagt tacccttaaa gtgttgatat
      481 gactgttttc aaacataaaa aaagttgctt tttcgtacct attaatgtat cgttttaaat
      541 gactagtaaa aaacatacat agaaagggga aaaagcaact tttttattat catagtttgt
      601 gaaaactaag ttgtttttat gtgttataac atggaaaagt atactgagaa aaaacaaaga
      661 aatcaagtat ttcagaaatt tattaaacgt catattggag agaatcaaat gaatttagtt
      721 gaagattgta atacatttct gtcttttgta gctgataaaa ctttagaaaa acagaaatta
      781 tataaagcta attcttgtaa aaatcgattt tgtcctgtct gcgcttggag aaaagctaga
      841 aaagacgcgt tgggtttatc tttgatgatg caatatatta agcagcaaca gaaaaaagaa
      901 tttatctttt taactttaac tacacctaac gtaatgagtg atgaattaga aaatgaaata
      961 aaacgttaca ataattcttt tagaaaactt ataaaaagaa aaaaagtaga tagtgttata
     1021 aagggatatg ttcgtaagtt agagattaca tataataaaa aaagagatga ttataaccct
     1081 cattttcacg tgttaattgc agtaaataaa tcgtatttca cagataaaag atattatatt
     1141 agccaacaag aatggttaga tttatggcgt gatgtaacag gtatttcaga aatcacacaa
     1201 gttcaagttc aaaaaataag acaaaacaat aataaagagt tatatgaaat ggctaagtat
     1261 tctggtaaag atagtgatta tttaataaat caaaaagtat ttgatgcatt ctataaatca
     1321 cttaaaggta aacaggtatt agtttattca ggattattta aagaggctaa aaagaaatta
     1381 aaaaatgggg atttagatta cttaaaagaa attgatccaa ccgaatatat ctatcaaatt
     1441 ttttatattt ggaaacaaaa agagtattta gctagtgaac tttatgactt aacagaacaa
     1501 gaaaaaagag aaattaatca caaaatgata gacgaaatcg aggaagaaca ataacaaaat
     1561 ataagtgcta acagctgacc tcccgataac accatgtagt tattgggagg tcagctgttg
     1621 aattatgcac gagtatttta aaagttattg tgatgacgac gataaacgat tatcaaaagt
     1681 ataatgttaa aatactttat tatactaacg ttatataaac attatacttt cgttatacaa
     1741 attttaaccc tgttaggaac tataaaaaat catgaaaatt ttaatttgca tgtaaatggg
     1801 ccagtgtcta aaaaaatcga cactggattt gctcaaattt ttgtttgtga aaagtaatta
     1861 cttttatttg gctctcattt gcgttttaag agccgatata ggtttttagg tataaaacta
     1921 tataaataac ccctaattct ttaaaataac cctttaaaat tcaaaataaa ggcataaaaa
     1981 ataaaagcta tctcaatttt gagatagctt ttttctttcg tacggtctgt ttatttttct
     2041 ttcgacgccg tatcgtcgat ggaattcaaa aaagttatgg gctataaatc tacatcactt
     2101 tttcaagaat gtagtagcaa tattcaagac aaattaattt ttataactgc tcggaatatc
     2161 tcaagccgtt tctttaattt tgaaataaaa aaatcgacgg aggtcgatta tggtttttgc
     2221 acgcgttctt tttagaacgc ataagtgcgc ccttacggga tttaactaga ttataacgac
     2281 gaaaatcaga cctgtaaagc gataactatt gtgataaagt ttgctaaaaa ggagattaaa
     2341 aaaatgctta tatttttatt tattttggtt tgtggtcttt taggttattt tttgattagt
     2401 cgttctaaaa aagattagac acgcatttat gccgagaaaa tttattgatg ttgagaagaa
     2461 cccttaacta aacttgcaga cgaatgtcgg catagcgtga gctattaagc cgaccattcg
     2521 acaagttttg ggattgttaa gggttccgag gctcaacgtc aataaagcaa ttggaataaa
     2581 ataatcaatg gctttctc
//
