LOCUS       CP127535                2473 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C344 plasmid pC344_1, complete
            sequence.
ACCESSION   CP127535
VERSION     CP127535.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360971
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C344
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 109x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 01:31:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,829
            CDSs (total)                      :: 2,750
            Genes (coding)                    :: 2,664
            CDSs (with protein)               :: 2,664
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 35 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 26 of 86
            Pseudo Genes (multiple problems)  :: 19 of 86
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C344"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC344_1"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2015-10-21"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 172"
     gene            complement(284..760)
                     /locus_tag="PCM64_14135"
     CDS             complement(284..760)
                     /locus_tag="PCM64_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WIY97854.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            complement(1208..1942)
                     /gene="erm(C)"
                     /locus_tag="PCM64_14140"
     ARG             complement(1212..1942)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2003..2062)
                     /gene="ermCL"
                     /locus_tag="PCM64_14145"
     CDS             complement(2003..2062)
                     /gene="ermCL"
                     /locus_tag="PCM64_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WIY97856.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     ARG             complement(1209..1942)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(275..760)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgttggttcc aaccaatagg aaattggaat aagcaattag tataatgaga gtataatgtt
       61 ggtataacgt tagtataatg atgctttttt tcattatatt ttttatgtac tttaaacctg
      121 cacgcttatg tgaattagaa aaagcttaat cgcatttcat agattgacct cccaataact
      181 acgtggtgtt attgggaggt caatctattt catttgcgtt aagcttttga tctacattaa
      241 gcgtgcaggt tttttgtcta tttgtagacg attttatgtt atactagtcc ttgaaagaat
      301 aataatcaga taatgcattt tcttgttttt catttgcctc ttgctcaaag ttcccaaatt
      361 cgagtaagag gtatttttgt ttttggtcgt cgcctctcat tagtagttca gggtttaaca
      421 ttaatactcc agtttttctt tttataatat ttccttcttc taagatttta agtgttgtta
      481 ttactgtttg tagacttgtt cctgtagctt ttgctatttc tcttgttgta gctatcattg
      541 tattgttact taagtggaca ttatctagga tatagttaac gattttaagt ttttttccgc
      601 caatcatatc taacatactt attaattgca ctatatatgc ctttacgaag ttaccagacg
      661 tttgtttacg gtataacttg tctacctcta tgacttctcc actttcttcg tctatgagcc
      721 tctgagagcc tttatagact gttccatatc tttctttcat ctttttctca ctccttattt
      781 taaactattc taactatatc ataactgttc taaaaaaaaa agaacatttg ttaaaagaaa
      841 ttagaacaaa atgagtgaaa aattagaaca aacaaattcc ttataaacct tatcatctca
      901 acctatatta agattttacc tagttgaatc ttcttttcta tataaagcgt cggagcatat
      961 cagggggtta tctaacgtaa atgctaccct tcggctcgct ttcgctcggc attgacgtca
     1021 gatactgcac cccctgaacc cccatgctcc aacagcaaaa aggaaacttt ttgctgcttt
     1081 tccgacgctt attcgcttcg ctcatattta tatagaaaag aagtgaatgc gcaaaagaca
     1141 taatcgattc acaaaaaata ggtacacgaa aaacaagtta agggatgcag tttatgcatc
     1201 ccttaactta cttattaaat aatttatagc tattgaaaag agataagaat tgttcaaagc
     1261 taatattgtt taaatcgtca attcctgcat gttttaagga attgttaaat tgattttttg
     1321 taaatatttt cttgtattct ttgttaaccc atttcataac gaaataatta tacttctgtt
     1381 tatctttgtg tgatattctt gatttttttc tatttaatct gataagtgag ctattcactt
     1441 taggtttagg atgaaaatat tctcttggaa ccatacttaa tatagaaata tcaacttctg
     1501 ccattaaaaa taatgccaat gagcgttttg tatttaataa tcttttagca aacccgtatt
     1561 ccacgattaa ataaatctca tcagctatac tatcaaaaac aattttgcgt attatatccg
     1621 tacttatgtt ataaggtata ttaccaaata ttttatagga ttggttttta ggaaatttaa
     1681 actgcaatat atccttgttt aaaacttgga aattatcgtg atcaacaagt ttattttctg
     1741 tagttttgca taatttatgg tctatttcaa tggcagttac gaaattacac ctctgtacta
     1801 attcaagggt aaaatgccct tttcctgagc cgatttcaaa gatattatca tgttcattta
     1861 atcttatatt tgtcattatt ttatctatat tatgttttga agtaataaag ttttgactgt
     1921 gttttatatt tttctcgttc attataaccc tctttaattt ggttataatg aattttgctt
     1981 attaacgatt cattataacc acttattttt tgtttggttg ataatgaact gtgctgatta
     2041 caaaaatact aaaaatgccc atattttttc ctccttataa aattagtata attatagcac
     2101 gagctctgat aaatatgaac atgatgagtg atcgttaaat ttatactgca atctgatgcg
     2161 attattgaat aaaagatatg agagatttat ctagtttctt tttttacaag aaaaaagaaa
     2221 gttcttaaag gttttatact tttggtcgta gagcacacgg tttaacgact taattacgaa
     2281 gtaaataagt ctagtgtgtt agactttaat gtttttttaa ggcattagtg catttaagcg
     2341 tcagagcatg gctttatgcc gagaaaacta ttggttggaa tggcgtgtgt gttagccaaa
     2401 gctttggcga gttggttggg ggtttcatgg gattaatccc atgaaagtac caactcaaca
     2461 acacactaac gcc
//
