LOCUS       CP127533                2473 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C247 plasmid pC247_1, complete
            sequence.
ACCESSION   CP127533
VERSION     CP127533.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360969
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C247
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 19x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 01:46:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,822
            CDSs (total)                      :: 2,743
            Genes (coding)                    :: 2,657
            CDSs (with protein)               :: 2,657
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 35 of 86
            Pseudo Genes (incomplete)         :: 46 of 86
            Pseudo Genes (internal stop)      :: 26 of 86
            Pseudo Genes (multiple problems)  :: 19 of 86
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C247"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC247_1"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2014-07-07"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 170"
     gene            275..334
                     /gene="ermCL"
                     /locus_tag="PCL56_14100"
     CDS             275..334
                     /gene="ermCL"
                     /locus_tag="PCL56_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WIY89913.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     gene            395..1129
                     /gene="erm(C)"
                     /locus_tag="PCL56_14105"
     ARG             396..1126
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1577..2053
                     /locus_tag="PCL56_14110"
     CDS             1577..2053
                     /locus_tag="PCL56_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WIY89911.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     ARG             396..1129
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          1578..2063
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaatgcact aatgccttaa aaaaacatta aagtctaaca cactagactt atttacttcg
       61 taattaagtc gttaaaccgt gtgctctacg accaaaagta taaaaccttt aagaactttc
      121 ttttttcttg taaaaaaaga aactagataa atctctcata tcttttattc aataatcgca
      181 tcagattgca gtataaattt aacgatcact catcatgttc atatttatca gagctcgtgc
      241 tataattata ctaattttat aaggaggaaa aaatatgggc atttttagta tttttgtaat
      301 cagcacagtt cattatcaac caaacaaaaa ataagtggtt ataatgaatc gttaataagc
      361 aaaattcatt ataaccaaat taaagagggt tataatgaac gagaaaaata taaaacacag
      421 tcaaaacttt attacttcaa aacataatat agataaaata atgacaaata taagattaaa
      481 tgaacatgat aatatctttg aaatcggctc aggaaaaggg cattttaccc ttgaattagt
      541 acagaggtgt aatttcgtaa ctgccattga aatagaccat aaattatgca aaactacaga
      601 aaataaactt gttgatcacg ataatttcca agttttaaac aaggatatat tgcagtttaa
      661 atttcctaaa aaccaatcct ataaaatatt tggtaatata ccttataaca taagtacgga
      721 tataatacgc aaaattgttt ttgatagtat agctgatgag atttatttaa tcgtggaata
      781 cgggtttgct aaaagattat taaatacaaa acgctcattg gcattatttt taatggcaga
      841 agttgatatt tctatattaa gtatggttcc aagagaatat tttcatccta aacctaaagt
      901 gaatagctca cttatcagat taaatagaaa aaaatcaaga atatcacaca aagataaaca
      961 gaagtataat tatttcgtta tgaaatgggt taacaaagaa tacaagaaaa tatttacaaa
     1021 aaatcaattt aacaattcct taaaacatgc aggaattgac gatttaaaca atattagctt
     1081 tgaacaattc ttatctcttt tcaatagcta taaattattt aataagtaag ttaagggatg
     1141 cataaactgc atcccttaac ttgtttttcg tgtacctatt ttttgtgaat cgattatgtc
     1201 ttttgcgcat tcacttcttt tctatataaa tatgagcgaa gcgaataagc gtcggaaaag
     1261 cagcaaaaag tttccttttt gctgttggag catgggggtt cagggggtgc agtatctgac
     1321 gtcaatgccg agcgaaagcg agccgaaggg tagcatttac gttagataac cccctgatat
     1381 gctccgacgc tttatataga aaagaagatt caactaggta aaatcttaat ataggttgag
     1441 atgataaggt ttataaggaa tttgtttgtt ctaatttttc actcattttg ttctaatttc
     1501 ttttaacaaa tgttcttttt tttttagaac agttatgata tagttagaat agtttaaaat
     1561 aaggagtgag aaaaagatga aagaaagata tggaacagtc tataaaggct ctcagaggct
     1621 catagacgaa gaaagtggag aagtcataga ggtagacaag ttataccgta aacaaacgtc
     1681 tggtaacttc gtaaaggcat atatagtgca attaataagt atgttagata tgattggcgg
     1741 aaaaaaactt aaaatcgtta actatatcct agataatgtc cacttaagta acaatacaat
     1801 gatagctaca acaagagaaa tagcaaaagc tacaggaaca agtctacaaa cagtaataac
     1861 aacacttaaa atcttagaag aaggaaatat tataaaaaga aaaactggag tattaatgtt
     1921 aaaccctgaa ctactaatga gaggcgacga ccaaaaacaa aaatacctct tactcgaatt
     1981 tgggaacttt gagcaagagg caaatgaaaa acaagaaaat gcattatctg attattattc
     2041 tttcaaggac tagtataaca taaaatcgtc tacaaataga caaaaaacct gcacgcttaa
     2101 tgtagatcaa aagcttaacg caaatgaaat agattgacct cccaataaca ccacgtagtt
     2161 attgggaggt caatctatga aatgcgatta agctttttct aattcacata agcgtgcagg
     2221 tttaaagtac ataaaaaata taatgaaaaa aagcatcatt atactaacgt tataccaaca
     2281 ttatactctc attatactaa ttgcttattc caatttccta ttggttggaa ccaacaggcg
     2341 ttagtgtgtt gttgagttgg tactttcatg ggattaatcc catgaaaccc ccaaccaact
     2401 cgccaaagct ttggctaaca cacacgccat tccaaccaat agttttctcg gcataaagcc
     2461 atgctctgac gct
//
