LOCUS       CP127531                2473 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C272 plasmid pC272_1, complete
            sequence.
ACCESSION   CP127531
VERSION     CP127531.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360970
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C272
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 90x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 01:31:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,828
            CDSs (total)                      :: 2,749
            Genes (coding)                    :: 2,662
            CDSs (with protein)               :: 2,662
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 46 of 87
            Pseudo Genes (internal stop)      :: 26 of 87
            Pseudo Genes (multiple problems)  :: 19 of 87
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C272"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC272_1"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2014-09-15"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 171"
     gene            55..531
                     /locus_tag="PCL38_14130"
     CDS             55..531
                     /locus_tag="PCL38_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WIZ08272.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            1226..1285
                     /gene="ermCL"
                     /locus_tag="PCL38_14135"
     CDS             1226..1285
                     /gene="ermCL"
                     /locus_tag="PCL38_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WIZ08273.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     gene            1346..2080
                     /gene="erm(C)"
                     /locus_tag="PCL38_14140"
     ARG             1347..2077
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1347..2080
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          56..541
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttagaacag ttatgatata gttagaatag tttaaaataa ggagtgagaa aaagatgaaa
       61 gaaagatatg gaacagtcta taaaggctct cagaggctca tagacgaaga aagtggagaa
      121 gtcatagagg tagacaagtt ataccgtaaa caaacgtctg gtaacttcgt aaaggcatat
      181 atagtgcaat taataagtat gttagatatg attggcggaa aaaaacttaa aatcgttaac
      241 tatatcctag ataatgtcca cttaagtaac aatacaatga tagctacaac aagagaaata
      301 gcaaaagcta caggaacaag tctacaaaca gtaataacaa cacttaaaat cttagaagaa
      361 ggaaatatta taaaaagaaa aactggagta ttaatgttaa accctgaact actaatgaga
      421 ggcgacgacc aaaaacaaaa atacctctta ctcgaatttg ggaactttga gcaagaggca
      481 aatgaaaaac aagaaaatgc attatctgat tattattctt tcaaggacta gtataacata
      541 aaatcgtcta caaatagaca aaaaacctgc acgcttaatg tagatcaaaa gcttaacgca
      601 aatgaaatag attgacctcc caataacacc acgtagttat tgggaggtca atctatgaaa
      661 tgcgattaag ctttttctaa ttcacataag cgtgcaggtt taaagtacat aaaaaatata
      721 atgaaaaaaa gcatcattat actaacgtta taccaacatt atactctcat tatactaatt
      781 gcttattcca atttcctatt ggttggaacc aacaggcgtt agtgtgttgt tgagttggta
      841 ctttcatggg attaatccca tgaaaccccc aaccaactcg ccaaagcttt ggctaacaca
      901 cacgccattc caaccaatag ttttctcggc ataaagccat gctctgacgc ttaaatgcac
      961 taatgcctta aaaaaacatt aaagtctaac acactagact tatttacttc gtaattaagt
     1021 cgttaaaccg tgtgctctac gaccaaaagt ataaaacctt taagaacttt cttttttctt
     1081 gtaaaaaaag aaactagata aatctctcat atcttttatt caataatcgc atcagattgc
     1141 agtataaatt taacgatcac tcatcatgtt catatttatc agagctcgtg ctataattat
     1201 actaatttta taaggaggaa aaaatatggg catttttagt atttttgtaa tcagcacagt
     1261 tcattatcaa ccaaacaaaa aataagtggt tataatgaat cgttaataag caaaattcat
     1321 tataaccaaa ttaaagaggg ttataatgaa cgagaaaaat ataaaacaca gtcaaaactt
     1381 tattacttca aaacataata tagataaaat aatgacaaat ataagattaa atgaacatga
     1441 taatatcttt gaaatcggct caggaaaagg gcattttacc cttgaattag tacagaggtg
     1501 taatttcgta actgccattg aaatagacca taaattatgc aaaactacag aaaataaact
     1561 tgttgatcac gataatttcc aagttttaaa caaggatata ttgcagttta aatttcctaa
     1621 aaaccaatcc tataaaatat ttggtaatat accttataac ataagtacgg atataatacg
     1681 caaaattgtt tttgatagta tagctgatga gatttattta atcgtggaat acgggtttgc
     1741 taaaagatta ttaaatacaa aacgctcatt ggcattattt ttaatggcag aagttgatat
     1801 ttctatatta agtatggttc caagagaata ttttcatcct aaacctaaag tgaatagctc
     1861 acttatcaga ttaaatagaa aaaaatcaag aatatcacac aaagataaac agaagtataa
     1921 ttatttcgtt atgaaatggg ttaacaaaga atacaagaaa atatttacaa aaaatcaatt
     1981 taacaattcc ttaaaacatg caggaattga cgatttaaac aatattagct ttgaacaatt
     2041 cttatctctt ttcaatagct ataaattatt taataagtaa gttaagggat gcataaactg
     2101 catcccttaa cttgtttttc gtgtacctat tttttgtgaa tcgattatgt cttttgcgca
     2161 ttcacttctt ttctatataa atatgagcga agcgaataag cgtcggaaaa gcagcaaaaa
     2221 gtttcctttt tgctgttgga gcatgggggt tcagggggtg cagtatctga cgtcaatgcc
     2281 gagcgaaagc gagccgaagg gtagcattta cgttagataa ccccctgata tgctccgacg
     2341 ctttatatag aaaagaagat tcaactaggt aaaatcttaa tataggttga gatgataagg
     2401 tttataagga atttgtttgt tctaattttt cactcatttt gttctaattt cttttaacaa
     2461 atgttctttt ttt
//
