LOCUS       CP127529                2473 bp    DNA     circular BCT 19-JUN-2023
DEFINITION  Staphylococcus aureus strain C347 plasmid pC347_1, complete
            sequence.
ACCESSION   CP127529
VERSION     CP127529.1
DBLINK      BioProject: PRJNA914892
            BioSample: SAMN32360972
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Hybrid Oxford Nanopore Technologies Illumina Sequences of 174
            Staphylococcus Aureus Isolates from Chronic Rhinosinusitis Patients
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Bouras,G., Houtak,G., Nepal,R., Shaghayegh,G. and Vreugde,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2023) Department of ENT Surgery at the Basil
            Hetzel Institute, Adelaide Medical School, The University of
            Adelaide, 37 Woodville Road, Woodville South, SA 5011, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 14-NOV-2022
            Assembly Method :: Flye for Genome, plassembler (Unicycler) for
            plasmids v. 2.9.1 (Flye) with short read polishing, 0.1.1
            (plassembler) with 0.5.0 (Unicycler)
            Assembly Name                     :: C347
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 19x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/11/2023 01:43:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,811
            CDSs (total)                      :: 2,732
            Genes (coding)                    :: 2,648
            CDSs (with protein)               :: 2,648
            Genes (RNA)                       :: 79
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 33 of 84
            Pseudo Genes (incomplete)         :: 45 of 84
            Pseudo Genes (internal stop)      :: 25 of 84
            Pseudo Genes (multiple problems)  :: 18 of 84
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="C347"
                     /isolation_source="Patient Sinus Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="pC347_1"
                     /geo_loc_name="Australia: Adelaide"
                     /lat_lon="34.9285 S 138.6007 E"
                     /collection_date="2015-11-11"
                     /collected_by="ENT Surgery Department at the Queen
                     Elizabeth Hospital, Adelaide, Australia 173"
     gene            complement(join(2217..2473,1..220))
                     /locus_tag="PCM14_14045"
     CDS             complement(join(2217..2473,1..220))
                     /locus_tag="PCM14_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_process:
                     GO:0006260 - DNA replication [Evidence IEA]; GO_process:
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WIY95190.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            complement(668..1402)
                     /gene="erm(C)"
                     /locus_tag="PCM14_14050"
     ARG             complement(672..1402)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1463..1522)
                     /gene="ermCL"
                     /locus_tag="PCM14_14055"
     CDS             complement(1463..1522)
                     /gene="ermCL"
                     /locus_tag="PCM14_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WIY95192.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     ARG             complement(669..1402)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          complement(2..220)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.56e-111"
     Typing          complement(2262..2473)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="98.0"
                     /evalue="5.42e-107"
ORIGIN
        1 tattgttact taagtggaca ttatctagga tatagttaac gattttaagt ttttttccgc
       61 caatcatatc taacatactt attaattgca ctatatatgc ctttacgaag ttaccagacg
      121 tttgtttacg gtataacttg tctacctcta tgacttctcc actttcttcg tctatgagcc
      181 tctgagagcc tttatagact gttccatatc tttctttcat ctttttctca ctccttattt
      241 taaactattc taactatatc ataactgttc taaaaaaaaa agaacatttg ttaaaagaaa
      301 ttagaacaaa atgagtgaaa aattagaaca aacaaattcc ttataaacct tatcatctca
      361 acctatatta agattttacc tagttgaatc ttcttttcta tataaagcgt cggagcatat
      421 cagggggtta tctaacgtaa atgctaccct tcggctcgct ttcgctcggc attgacgtca
      481 gatactgcac cccctgaacc cccatgctcc aacagcaaaa aggaaacttt ttgctgcttt
      541 tccgacgctt attcgcttcg ctcatattta tatagaaaag aagtgaatgc gcaaaagaca
      601 taatcgattc acaaaaaata ggtacacgaa aaacaagtta agggatgcag tttatgcatc
      661 ccttaactta cttattaaat aatttatagc tattgaaaag agataagaat tgttcaaagc
      721 taatattgtt taaatcgtca attcctgcat gttttaagga attgttaaat tgattttttg
      781 taaatatttt cttgtattct ttgttaaccc atttcataac gaaataatta tacttctgtt
      841 tatctttgtg tgatattctt gatttttttc tatttaatct gataagtgag ctattcactt
      901 taggtttagg atgaaaatat tctcttggaa ccatacttaa tatagaaata tcaacttctg
      961 ccattaaaaa taatgccaat gagcgttttg tatttaataa tcttttagca aacccgtatt
     1021 ccacgattaa ataaatctca tcagctatac tatcaaaaac aattttgcgt attatatccg
     1081 tacttatgtt ataaggtata ttaccaaata ttttatagga ttggttttta ggaaatttaa
     1141 actgcaatat atccttgttt aaaacttgga aattatcgtg atcaacaagt ttattttctg
     1201 tagttttgca taatttatgg tctatttcaa tggcagttac gaaattacac ctctgtacta
     1261 attcaagggt aaaatgccct tttcctgagc cgatttcaaa gatattatca tgttcattta
     1321 atcttatatt tgtcattatt ttatctatat tatgttttga agtaataaag ttttgactgt
     1381 gttttatatt tttctcgttc attataaccc tctttaattt ggttataatg aattttgctt
     1441 attaacgatt cattataacc acttattttt tgtttggttg ataatgaact gtgctgatta
     1501 caaaaatact aaaaatgccc atattttttc ctccttataa aattagtata attatagcac
     1561 gagctctgat aaatatgaac atgatgagtg atcgttaaat ttatactgca atctgatgcg
     1621 attattgaat aaaagatatg agagatttat ctagtttctt tttttacaag aaaaaagaaa
     1681 gttcttaaag gttttatact tttggtcgta gagcacacgg tttaacgact taattacgaa
     1741 gtaaataagt ctagtgtgtt agactttaat gtttttttaa ggcattagtg catttaagcg
     1801 tcagagcatg gctttatgcc gagaaaacta ttggttggaa tggcgtgtgt gttagccaaa
     1861 gctttggcga gttggttggg ggtttcatgg gattaatccc atgaaagtac caactcaaca
     1921 acacactaac gcctgttggt tccaaccaat aggaaattgg aataagcaat tagtataatg
     1981 agagtataat gttggtataa cgttagtata atgatgcttt ttttcattat attttttatg
     2041 tactttaaac ctgcacgctt atgtgaatta gaaaaagctt aatcgcattt catagattga
     2101 cctcccaata actacgtggt gttattggga ggtcaatcta tttcatttgc gttaagcttt
     2161 tgatctacat taagcgtgca ggttttttgt ctatttgtag acgattttat gttatactag
     2221 tccttgaaag aataataatc agataatgca ttttcttgtt tttcatttgc ctcttgctca
     2281 aagttcccaa attcgagtaa gaggtatttt tgtttttggt cgtcgcctct cattagtagt
     2341 tcagggttta acattaatac tccagttttt ctttttataa tatttccttc ttctaagatt
     2401 ttaagtgttg ttattactgt ttgtagactt gttcctgtag cttttgctat ttctcttgtt
     2461 gtagctatca ttg
//
