LOCUS       CP127026                8681 bp    DNA     linear   BCT 29-FEB-2024
DEFINITION  Staphylococcus aureus strain CC8-MSSA (PVL+) isolate
            Trinidad&Tobago_2020-043_7352M plasmid unnamed1.
ACCESSION   CP127026
VERSION     CP127026.1
DBLINK      BioProject: PRJNA978032
            BioSample: SAMN35540727
KEYWORDS    .
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Monecke,S., Akpaka,P.E., Smith,M.R., Unakal,C.G., Thoms
            Rodriguez,C.A., Ashraph,K., Muller,E., Braun,S.D., Diezel,C.,
            Reinicke,M. and Ehricht,R.
  TITLE     Clonal Complexes Distribution of Staphylococcus aureus Isolates from
            Clinical Samples from the Caribbean Islands
  JOURNAL   Antibiotics (Basel) 12 (6), 1050 (2023)
   PUBMED   37370368
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   Diezel,C., Braun,S.D., Reinicke,M. and Monecke,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-JUN-2023) Optisch-Molekulare Diagnostik und
            Systemtechnologien, Leibniz Institute of Photonic Technology (IPHT),
            Philosophenweg 7, Jena 07743, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.8.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 142x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 06/05/2023 11:44:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,895
            CDSs (total)                      :: 2,813
            Genes (coding)                    :: 2,575
            CDSs (with protein)               :: 2,575
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 238
            CDSs (without protein)            :: 238
            Pseudo Genes (ambiguous residues) :: 0 of 238
            Pseudo Genes (frameshifted)       :: 202 of 238
            Pseudo Genes (incomplete)         :: 40 of 238
            Pseudo Genes (internal stop)      :: 17 of 238
            Pseudo Genes (multiple problems)  :: 19 of 238
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="CC8-MSSA (PVL+)"
                     /isolate="Trinidad&Tobago_2020-043_7352M"
                     /isolation_source="Medical/diagnostic sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed1"
                     /geo_loc_name="Trinidad and Tobago"
                     /collection_date="2019-11-24"
     gene            212..535
                     /locus_tag="QQO12_14260"
                     /pseudo=""
     CDS             212..535
                     /locus_tag="QQO12_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /name="phage holin, lambda family"
     gene            519..995
                     /locus_tag="QQO12_14265"
     CDS             519..995
                     /locus_tag="QQO12_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003796 - lysozyme activity [Evidence IEA]; GO_process:
                     GO:0009253 - peptidoglycan catabolic process [Evidence
                     IEA]; GO_process: GO:0016998 - cell wall macromolecule
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="WIO64448.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTDY
                     SDHPRKLVTLNPKLKSTGAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKERGA
                     LPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
                     /name="glycoside hydrolase family protein"
     gene            992..1453
                     /gene="rzpD"
                     /locus_tag="QQO12_14270"
     CDS             992..1453
                     /gene="rzpD"
                     /locus_tag="QQO12_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage endopeptidase RzpD"
                     /protein_id="WIO64449.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKAQRDKNARELK
                     LANAAITDMQMRQRDVAALDAKYTKELADAKAENDALRDDVAAGRRRLHIKAVCQSVRE
                     ATTASGVDNAASPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
                     /name="rzpD"
     gene            complement(1487..1780)
                     /gene="bor"
                     /locus_tag="QQO12_14275"
     CDS             complement(1487..1780)
                     /gene="bor"
                     /locus_tag="QQO12_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="WIO64450.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKPAAVAPKETITHHFFVSG
                     IGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
                     /name="bor"
     gene            complement(2071..2481)
                     /locus_tag="QQO12_14280"
     CDS             complement(2071..2481)
                     /locus_tag="QQO12_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="WIO64451.1"
                     /translation="MAQVAIFKEIFDQVRKDLDCELFYSELKRHNVSHYIYYLATDNIH
                     IVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFRWV
                     TNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
                     /name="DUF1398 domain-containing protein"
     gene            2767..2973
                     /gene="ybcW"
                     /locus_tag="QQO12_14285"
     CDS             2767..2973
                     /gene="ybcW"
                     /locus_tag="QQO12_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="WIO64452.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLEA
                     FRAIEAALVKHDNNMKDYSLVVD"
                     /name="ybcW"
     gene            complement(3138..3332)
                     /locus_tag="QQO12_14290"
     CDS             complement(3138..3332)
                     /locus_tag="QQO12_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="WIO64453.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEAC
                     RRRLTSEKRAYTSIKSDEE"
                     /name="YlcI/YnfO family protein"
     gene            complement(3604..4167)
                     /locus_tag="QQO12_14295"
                     /pseudo=""
     CDS             complement(3604..4167)
                     /locus_tag="QQO12_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /name="PASTA domain-containing protein"
     gene            5844..6050
                     /gene="murD"
                     /locus_tag="QQO12_14300"
                     /pseudo=""
     CDS             5844..6050
                     /gene="murD"
                     /locus_tag="QQO12_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637171.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /name="murD"
     gene            6207..6618
                     /locus_tag="QQO12_14305"
                     /pseudo=""
     CDS             6207..6618
                     /locus_tag="QQO12_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /name="DUF1398 domain-containing protein"
     gene            6908..7202
                     /gene="bor"
                     /locus_tag="QQO12_14310"
                     /pseudo=""
     CDS             6908..7202
                     /gene="bor"
                     /locus_tag="QQO12_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /name="bor"
     gene            complement(7234..7695)
                     /gene="rzpD"
                     /locus_tag="QQO12_14315"
     CDS             complement(7234..7695)
                     /gene="rzpD"
                     /locus_tag="QQO12_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage endopeptidase RzpD"
                     /protein_id="WIO64454.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKAQRDKNARELK
                     LANAAITDMQMRQRDVAALDAKYTKELADAKAENDALRDDVAAGRRRLHIKAVCQSVRE
                     ATTASGVDNAASPRLADTAERDYFTLRERLITMQKQLEGTQKYINEQCR"
                     /name="rzpD"
     gene            complement(7692..8168)
                     /locus_tag="QQO12_14320"
     CDS             complement(7692..8168)
                     /locus_tag="QQO12_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003796 - lysozyme activity [Evidence IEA]; GO_process:
                     GO:0009253 - peptidoglycan catabolic process [Evidence
                     IEA]; GO_process: GO:0016998 - cell wall macromolecule
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="WIO64455.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTDY
                     SDHPRKLVTLNPKLKSTGAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKERGA
                     LPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
                     /name="glycoside hydrolase family protein"
     gene            complement(8152..8474)
                     /locus_tag="QQO12_14325"
                     /pseudo=""
     CDS             complement(8152..8474)
                     /locus_tag="QQO12_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_process: GO:0019835 - cytolysis
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /name="phage holin, lambda family"
ORIGIN
        1 attgctgcca tcagattgtg tttgttagtc gctttttttt tggatttttt tttttggaat
       61 tttttttgcg ctaacaacct cctgccgttt tgcccgtgca tatcggtcac gaacaaatct
      121 gattactaaa cacagtagcc tggatttgtt ctatcagtaa tcgaccttat tcctaattaa
      181 atagagcaaa tccccttatt gggggtaaga catgaagatg ccagaaaaac atgacctgtt
      241 ggccgccatt ctcgcggcaa aggaacaagg catcggggca atccttgcgt ttgcaatggc
      301 gtaccttcgc ggcagatata atggcggtgc gtttacaaaa acagtaatcg acgcaacgat
      361 gtgcgccatt atcgcctagt tcattcgtga ccttctcgac ttcgccggac taagtagcaa
      421 tctcgcttat ataacgagcg tgtttatcgg ctacatcggt actgactcga ttggttcgct
      481 tatcaaacgc ttcgctgcta aaaaagccgg agtagaagat ggtagaaatc aataatcaac
      541 gtaaggcgtt cctcgatatg ctggcgtggt cggagggaac tgataacgga cgtcagaaaa
      601 ccagaaatca tggttatgac gtcattgtag gcggagagct atttactgat tactccgatc
      661 accctcgcaa acttgtcacg ctaaacccaa aactcaaatc aacaggcgcc ggacgctacc
      721 agcttctttc ccgttggtgg gatgcctacc gcaagcagct tggcctgaaa gacttctctc
      781 cgaaaagtca ggacgctgtg gcattgcagc agattaagga gcgtggcgct ttacctatga
      841 ttgatcgtgg tgatatccgt caggcaatcg accgttgcag caatatctgg gcttcactgc
      901 cgggcgctgg ttatggtcag ttcgagcata aggctgacag cctgattgca aaattcaaag
      961 aagcgggcgg aacggtcaga gagattgatg tatgagcaga gtcaccgcga ttatctccgc
     1021 tctggttatc tgcatcatcg tctgcctgtc atgggctgtt aatcattacc gtgataacgc
     1081 cattacctac aaagcccagc gcgacaaaaa tgccagagaa ctgaagctgg cgaacgcggc
     1141 aattactgac atgcagatgc gtcagcgtga tgttgctgcg ctcgatgcaa aatacacgaa
     1201 ggagttagct gatgctaaag ctgaaaatga tgctctgcgt gatgatgttg ccgctggtcg
     1261 tcgtcggttg cacatcaaag cagtctgtca gtcagtgcgt gaagccacca ccgcctccgg
     1321 cgtggataat gcagcctccc cccgactggc agacaccgct gaacgggatt atttcaccct
     1381 cagagagagg ctgatcacta tgcaaaaaca actggaagga acccagaagt atattaatga
     1441 gcagtgcaga tagagttgcc catatcgatg ggcaactcat gcaatattat tgtgagcaat
     1501 acacacgcgc ttccagcgga gtataaatgc ctaaagtaat aaaaccgagc aatccattta
     1561 cgaatgtttg ctgggtttct gttttaacaa cattttctgc gccgccacaa attttggctg
     1621 catcgacagt tttcttctgc ccaattccag aaacgaagaa atgatgggtg atggtttcct
     1681 ttggtgctac tgctgccggt ttgttttgaa cagtaaacgt ctgttgagca catcctgtaa
     1741 taagcagggc cagcgcagta gcgagtagca tttttttcat ggtgttattc ccgatgcttt
     1801 ttgaagttcg cagaatcgta tgtgtagaaa attaaacaaa ccctaaacaa tgagttgaaa
     1861 tttcatattg ttaatattta ttaatgtatg tcaggtgcga tgaatcgtca ttgtattccc
     1921 ggattaacta tgtccacagc cctgacgggg aacttctctg cgggagtgtc cgggaataat
     1981 taaaacgatg acacacaggg tttagcgcgt acacgtattg cattatgcca acgccccggt
     2041 gctgacacgg aagaaaccgg acgttatgat ttagcgtgga aagatttgtg tagtgttctg
     2101 aatgctctca gtaaatagta atgaattatc aaaggtatag taatatcttt tatgttcatg
     2161 gatatttgta acccatcgga aaactcctgc tttagcaaga ttttccctgt attgctgaaa
     2221 tgtgatttct cttgatttca acctatcata ggacgtttct ataagatgcg tgtttcttga
     2281 gaatttaaca tttacaacct ttttaagtcc ttttattaac acggtgttat cgttttctaa
     2341 cacgatgtga atattatctg tggctagata gtaaatataa tgtgagacgt tgtgacgttt
     2401 tagttcagaa taaaacaatt cacagtctaa atcttttcgc acttgatcga atatttcttt
     2461 aaaaatggca acctgagcca ttggtaaaac cttccatgtg atacgagggc gcgtagtttg
     2521 cattatcgtt tttatcgttt caatctggtc tgacctcctt gtgttttgtt gatgatttat
     2581 gtcaaatatt aggaatgttt tcacttaata gtattggttg cgtaacaaag tgcggtcctg
     2641 ctggcattct ggagggaaat acaaccgaca gatgtatgta aggccaacgt gctcaaatct
     2701 tcatacagaa agatttgaag taatatttta accgctagat gaagagcaag cgcatggagc
     2761 gacaaaatga ataaagaaca atctgctgat gatccctccg tggatctgat tcgtgtaaaa
     2821 aatatgctta atagcaccat ttctatgagt taccctgatg ttgtaattgc atgtatagaa
     2881 cataaggtgt ctctggaagc attcagagca attgaggcag cgttggtgaa gcacgataat
     2941 aatatgaagg attattccct ggtggttgac tgatcaccat aactgctaat cattcaaact
     3001 atttagtctg tgacagagcc aacacgcagt ctgtcactgt caggaaagtg gtaaaactgc
     3061 aactcaatta ctgcaatgcc ctcgtaatta agtgaattta caatatcgtc ctgttcggag
     3121 ggaagaacgc gggatgttca ttcttcatca cttttaattg atgtatatgc tctcttttct
     3181 gacgttagtc tccgacggca ggcttcaatg acccaggctg agaaattccc ggaccctttt
     3241 tgctcaagag cgatgttaat ttgttcaatc atttggttag gaaagcggat gttgcgggtt
     3301 gttgttctgc gggttctgtt cttcgttgac atgaggttgc cccgtattca gtgtcgctga
     3361 tttgtattgt ctgaagttgt ttttacgtta agttgatgca gatcaattaa tacgatacct
     3421 gcgtcataat tgattatttg acgtggtttg atggcctcca cgcacgttgt gatatgtaga
     3481 tgataatcat tatcacttta cgggtccttt ccggtgaaaa aaaggtacca aaaaaaacat
     3541 cgtcgtgagt agtgaaccgt aactgtcaga tttcttctta tcatctgaat ttgaatcaag
     3601 atgttaaatc gaattactgt ctacattctc tgttgaaaat ttctacgtct atttttatct
     3661 tttcagtaag ttttgtcctt actaattgat tggtgggaca caaagccgct acctttaaat
     3721 ttactttaat atttgttagg ttttctaaag caatgacatc ttctttcgtc catcctgaca
     3781 tgtcaggcat agttaagtct ccatctgtta ataacagtac aatactatga ggtaagactt
     3841 tattccctgc tttttataga ttgtgctttt tatttgtgtg ccagaaccaa tggtaactgg
     3901 ttctaatgat tttacactca cattatcaag cttttttgtt tgtcttgacc ttcaacatct
     3961 ggcttttact atactctgca ttagatgtgt catcttacgg aattacattt aaatatttca
     4021 aaagtatttt ccattattgg tttaaacgct ttacaacacc taattcataa gcttcttggt
     4081 catttttgtg tgccaagctc atataatata caataacttt aggattttct tcggcgcgtc
     4141 acccataaaa cttacaaaat atgggtgacg gcatatctaa gtgtgcaatg aagctggcct
     4201 cgcgtgttaa actataaaat taggtgttga ataaaaacgt ttaagtaata atggaaaatt
     4261 agaaaatctt tgatgtaggt aaatcaaaaa gatgtttaga aattgccagt gttgaaggtg
     4321 gacaaacaca aaatattgat aatgtgagtg caaaatttcc agttatattg gattctgaca
     4381 ccctaaataa agcaatcaaa agcagggctc ttacacatag taaagtactg ttattaacga
     4441 atgaaggcat aatatgcctg tgaatggaca atagaatacc taacaaagta aagtaaagaa
     4501 ggtaacattt atgtcatatc aagtaaaagc tactgaaaga taaaatggaa acgtaaatcg
     4561 aaatatagac aacaattcga caatattctg attcaaatag aaatctgaca gtaaaactga
     4621 cgaaaattcg gattctttat gaagcagcag gagccaggat gtgtgcgtca ctaatatttt
     4681 tactaagcaa tgcttgtgat agaaaaacgt aaataactca ttcatagaaa taagtgttga
     4741 tagagaattt taaggtagac ggataagaac aacgacatta caaggcaact ttatacttaa
     4801 tactatatat aaagaaaatt acaaattttg attacaactt tatttgttga aaattattaa
     4861 tatattagtg gttaaaaatg caattgaaaa ggggagctat gtttcaggat tttttgtata
     4921 tacgttatgc cttagtgata cgactgtttt ggtacactgt ttgtatacat taaaaggatg
     4981 aaatttggtc tagaagaaga tccacaaagc catatgaaga gactggtata gcgatgggat
     5041 agactattat ttctttattt aaatattaat gatatattat cagataagca atcaatcatt
     5101 gctatgatta ttatttgtga ctatttgctt agtgagtttt ataaatgatt atattattgt
     5161 attaagaaaa caatagcttt aacaagtaaa cataattttg gcatttaaat tgtatttaca
     5221 ttatttgctt tgttttaaat attgtgtttc gctattaatt ttctacgagt atattccatt
     5281 tacggaaaat aatccctaaa cctcaaattc attttttcat tgatcatttt tggcaaatag
     5341 aattttttgt acggcaaaat attaacagat ggtttagata gttagcaact gaaagactgt
     5401 caggcattat cattttttgt atgccatcat gaaacgatgt tggaaaacga gtgtattact
     5461 actgtatcgt atatttgttt gcacttttac accatattta ttaacccctg ctagagtgtt
     5521 tacataagga gatttttagg caacttagca ggtaatcgta atcaggaatt atcgttgagc
     5581 tttttaggtt tagtattcat aattgaaaac atatattaca agtcataact agaaaagcat
     5641 atatttaaaa cggaagtccg atcattttag tgataggaaa atggtagaat gaagaaatta
     5701 atttttatta tttgatacga tatttgactt caggttttaa tcggtttatg gagcttgaaa
     5761 taaaatagga tatttattac tacaaattaa aaaaaataaa aataaattag tgtcggtttg
     5821 gctttcctaa aaataatgaa acattattaa ataagtcagg catgcgaata tatgtcaatg
     5881 acggaagaac cccaccatac gggtattctc atgcaaaaga tttagaatct atgggcattt
     5941 ctaatgatca atattttagc atgatatgtc caatagtatt aaaaaattat acaatatctt
     6001 gtattgatga agcagtgaaa acgggtttga aaattttaac agaagttgag ggttaaatta
     6061 tctaatctct gaagaagcac aatcattagc tttgtaacag ctgctcgaca gttactatac
     6121 aattggagat gtttaaaaaa aaaaatcctt taactagaat tatatcagca atattattat
     6181 attatacaaa atttaaggtt ttaccaatgg ctcaggttgc catttttaaa aaaaatattc
     6241 gatcaagtgc gaaaagattt agactgtgaa ttgttttatt ctgaactaaa acgtcacaac
     6301 gtctcacatt atatttacta tctagccaca gataatattc acatcgtgtt agaaaacgat
     6361 aacaccgtgt taataaaagg acttaaaaag gttgtaaatg ttaaattctc aagaaacacg
     6421 catcttatag aaacgtccta tgataggttg aaatcaagag aaatcacatt tcagcaatac
     6481 agggaaaatc ttgctaaagc aggagttttc cgatgggtta caaatatcca tgaacataaa
     6541 agatattact atacctttga taattcatta ctatttactg agagcattca gaacactaca
     6601 caaatctttc cacgctaaat cataacgtcc ggtttcttcc gtgtcagcac cggggcgttg
     6661 gcataatgca atacgtgtac gcgctaaacc ctgtgtgcat cgttttaatt attcccggac
     6721 actcccgcag agaagttccc cgtcagggct gtggacatag ttaatccggg aatacaatga
     6781 cgattcatcg cacctgacat acattaataa atattaacaa tatgaaattt caactcattg
     6841 tttagggttt gtttaatttt ctacacatac gattctgcga acttcaaaaa gcatcgggaa
     6901 taacaccatg aaaaaaatgc tactcgctac tgcgctggcc ctgcttatta caggatgtgc
     6961 tcaacagacg tttactgttc aaaacaaacc ggcagcagta gcaccaaagg aaaccatcac
     7021 ccatcatttc ttctgtttct ggaattgggc agaagaaaac tgtcgatgca gccaaaattt
     7081 gtggcggcgc agaaaatgtt gttaaaacag aaacccagca aacattcgta aatggattgc
     7141 tcggttttat tactttaggc atttatactc cgctggaagc gcgtgtgtat tgctcacaat
     7201 aattgcatga gttgcccatc gatatgggca actctatctg cactgctcat taatatactt
     7261 ctgggttcct tccagttgtt tttgcatagt gatcagcctc tctctgaggg tgaaataatc
     7321 ccgttcagcg gtgtctgcca gtcgggggga ggctgcatta tccacgccgg aggcggtggt
     7381 ggcttcacgc actgactgac agactgcttt gatgtgcaac cgacgacgac cagcggcaac
     7441 atcatcacgc agagcatcat tttcagcttt agcatcagct aactccttcg tgtattttgc
     7501 atcgagcgca gcaacatcac gctgacgcat ctgcatgtca gtaattgccg cgttcgccag
     7561 cttcagttct ctggcatttt tgtcgcgctg ggctttgtag gtaatggcgt tatcacggta
     7621 atgattaaca gcccatgaca ggcagacgat gatgcagata accagagcgg agataatcgc
     7681 ggtgactctg ctcatacatc aatctctctg accgttccgc ccgcttcttt gaattttgca
     7741 atcaggctgt cagccttatg ctcgaactga ccataaccag cgcccggcag tgaagcccag
     7801 atattgctgc aacggtcgat tgcctgacgg atatcaccac gatcaatcat aggtaaagcg
     7861 ccacgctcct taatctgctg caatgccaca gcgtcctgac ttttcggaga gaagtctttc
     7921 aggccaagct gcttgcggta ggcatcccac caacgggaaa gaagctggta gcgtccggcg
     7981 cctgttgatt tgagttttgg gtttagcgtg acaagtttgc gagggtgatc ggagtaatca
     8041 gtaaatagct ctccgcctac aatgacgtca taaccatgat ttctggtttt ctgacgtccg
     8101 ttatcagttc cctccgacca cgccagcata tcgaggaacg ccttacgttg attattgatt
     8161 tctaccatct tctactccgg ctttttagca gcgaagcgtt tgataagcga accaatcgag
     8221 tcagtaccga tgtagccgat aaacacgctc gttatataag cgagattgct acttagtccg
     8281 gcgaagtcga gaaggtcacg aatgaactag gcgataatgg cgcacatcgt tgcgtcgatt
     8341 actgtttttg taaacgcacc gccattatat ctgccgcgaa ggtacgccat tgcaaacgca
     8401 aggattgccc cgatgccttg ttcctttgcc gcgagaatgg cggccaacag gtcatgtttt
     8461 tctggcatct tcatgtctta ccccaataag gggatttgct ctatttaatt aggaataagg
     8521 tcgattactg atagaacaaa tccaggctac tgtgtttagt aatcagattt gttcgtgacc
     8581 gatatgcacg ggcaaaacgg caggaggttg ttagcgcaaa aaaaaattcc aaaaaaaaaa
     8641 ttccaaaaaa aaaagcgact aacaaacaca atctgatggc a
//
