LOCUS       CP125757                2933 bp    DNA     circular BCT 01-JUN-2023
DEFINITION  Klebsiella pneumoniae strain NU-CRE166 plasmid pNUCRE-166_7,
            complete sequence.
ACCESSION   CP125757
VERSION     CP125757.1
DBLINK      BioProject: PRJNA395086
            BioSample: SAMN17600204
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Cheung,B.H., Alisoltani,A., Kochan,T.J., Lebrun-Corbin,M.,
            Nozick,S.H., Bachta,K.E.R., Ozer,E.A. and Hauser,A.R.
  TITLE     Genome-wide screens reveal shared and strain-specific genes
            necessary for enteric colonization by Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Ozer,E.A., Lopez,F.K., Bulman,Z.P. and Hauser,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) Medicine, Northwestern University Feinberg
            School of Medicine, 645 N Michigan Ave, Suite 900, Chicago, IL
            60611, USA
REFERENCE   3  (bases 1 to 2933)
  AUTHORS   Cheung,B.H., Kochan,T.J., Ozer,E.A. and Hauser,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) Microbiology-Immunology, Northwestern
            University, 303 E Chicago Ave, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/15/2023 16:16:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,987
            CDSs (total)                      :: 5,863
            Genes (coding)                    :: 5,715
            CDSs (with protein)               :: 5,715
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 65 of 148
            Pseudo Genes (incomplete)         :: 93 of 148
            Pseudo Genes (internal stop)      :: 18 of 148
            Pseudo Genes (multiple problems)  :: 25 of 148
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Alan Hauser (Northwestern
            University, 303 E Chicago Ave, Chicago, IL, USA).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NU-CRE166"
                     /isolation_source="bronchoalveolar lavage"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pNUCRE-166_7"
                     /geo_loc_name="USA: Chicago, IL"
                     /lat_lon="41.8945 N 87.6208 W"
                     /collection_date="2014-02"
     gene            complement(314..556)
                     /locus_tag="JNR65_029915"
     CDS             complement(314..556)
                     /locus_tag="JNR65_029915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WIE26180.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     gene            complement(694..1041)
                     /locus_tag="JNR65_029920"
     CDS             complement(694..1041)
                     /locus_tag="JNR65_029920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WIE26181.1"
                     /translation="MIMILWWISSFITHMMKVGNVREYGPTKSHLLLMVTTLPAGVGVF
                     IYPIVEVIWGDLSLLKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIA
                     GLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(1056..1433)
                     /locus_tag="JNR65_029925"
     CDS             complement(1056..1433)
                     /locus_tag="JNR65_029925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WIE26182.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLRIESK"
                     /name="hypothetical protein"
     gene            2282..2473
                     /locus_tag="JNR65_029930"
     CDS             2282..2473
                     /locus_tag="JNR65_029930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WIE26178.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDSLQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2473..2895
                     /locus_tag="JNR65_029935"
     CDS             2473..2895
                     /locus_tag="JNR65_029935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WIE26179.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     VSELIRVFGSLDGVTDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPSHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(1759..2124)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.095"
                     /coverage="85.0"
                     /evalue="1.36e-162"
ORIGIN
        1 agagtttctg aaccttccca aaaatcgcca gatctctgaa cgctcctcac cactaaaaat
       61 acccataccc acaccccacg caaaattaaa attttgcaga ttcattattt acaacaatgg
      121 gttaatgaaa aataaatgtg cggtgatgtg ttacaaagtg cagatccaag tcgtggacga
      181 ctctatggcg aatcgctgac gaattgccca cgattcatat gtgaatcacg ctttattatc
      241 atattgttat gatgaatcga ctcttgattc aattcatcac tttgggtgtt ttttgctcat
      301 ggtgactctt gattcaattc acgattcgaa tcgccggtga atcgtgtggt gagtcgtccg
      361 gcgacttgcc ataacgtgat tgccggccgt cgacgaaccc gattcctgaa ggtgctgaaa
      421 accggttttc cggctgcgcc ggcaacgctt cacggccacg caataaattg cgtccccgtt
      481 caccgttttc ggaggagaag aacgccggaa acaggacttc ccctggctga atggtgtgaa
      541 tttccgcgct atgcacttgc gcgcatactc atgcatgccg taaaaacaga gcctgcgcgt
      601 ttctggcggg ttttcgggtg gtttgttgcc tgttttaccg gtttcccgtc agaaacgccc
      661 tgagggcctc tcaggcggtg ctgataacgg atgttatggt aaatgttgga tagcaaggac
      721 tatcagtccg gcaatgctac atatcattgc taatggattt acgagcaaaa tgccgaatgt
      781 tgtcgcagat aatccgcgaa caatgatatt ggcaagaagt ccgccaacaa tgagggataa
      841 aaaggtataa ccagcaactt tcagtaacga cagatctccc catatcacct ctactatcgg
      901 atagataaaa acaccaacgc cagcaggaag cgtagtaacc atcaacagta aatggctttt
      961 agtgggaccg tactctctga catttccaac tttcatcatg tgtgtgatga atgacgatat
     1021 ccaccatagg atcatgatca taaggaaatt cataattatt tactctcgat gcgtagatca
     1081 ggtgctttct gtagatttgg tcgtacatac tcgctgtctc tttcgtcaag acaacggaag
     1141 tagagtgtta ctgtggtatc tatccccatt cttgtgttag ccacgttttc gttagtgagc
     1201 aggaattttt taccgaggct attacatttc tcagaacctg ttttaaaggc actttgacgt
     1261 gctgatgccg catctctaaa accatcagca gttgctgaaa ccgtatatgt atccctacct
     1321 aactcaagaa cgttgctggt agaaacacaa ccaactgtaa gcagcgatat tgataaaata
     1381 cctaacctat aaatcattga ttttattacc caagttgggt gattgtggat catgattata
     1441 tctgtttttt ttattattaa aatcaagggg ttcaataatt ttcttcaaag aactggtgtc
     1501 gtggagtggt gctggtttcc ctttgggggg gagagggatg gaataggatc ctcttgagat
     1561 cctttctgtt gcgtcgtaat ctcttgccct ggaaacgaaa aaaccgccca gcaaggcggt
     1621 tttttcgaag gttcttagag cagcaactct ttgaaccgag gtaactggct ttcgcagagc
     1681 acagaaacca aattagttat tacagtgtag ctgtacttca cctcacactt caacacttct
     1741 ttgttctgcg aatccgttac cagtggctgc tgccagtggc gataagtcgt gtctttccgg
     1801 attggactca agacgatagt taccggataa ggcgcagcag tcggactgaa cggggggttc
     1861 gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat
     1921 gagataaacg cggccataac agcggagtga caccggtaaa ccgaaaggca ggaacaggag
     1981 agcgcacgag ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2041 gccaccactg atttgagcgt ccgtttctgc gatgcttgtc aggggggcgg agcctatgga
     2101 aaaacggcaa cgccgtggcc tttccttttt ttcctccttt gaccgctcgg ttttctcgag
     2161 cttttctatg catgctcctg ccgctcgccg cagccgaacg accggagcgt agcgactgag
     2221 tgagcgagga agcggaaaag agacaggttt tacactgagc actgacgctc tgaggccctt
     2281 tatgacgaaa caacagaaaa cggcgctgaa catggctaaa ttcatccaga atcagtctct
     2341 gctcctgctg gagaagctta acgagcttga tcttgatgct gaggccgacc tttgtgagaa
     2401 actccacgat gatgcagagc atcttttccg tacactctcc tcgagactgg attcattaca
     2461 ggatggtaac tgatggccag agtgtctatt agtgaagctg cacggcttgt taaggttagt
     2521 cgtcccacta tttacaagat gattaacagc gggaagttaa gttatacctc tgttgtaaag
     2581 cacgggaagg atattaaagt tattgatgta tccgagctta ttagggtgtt tggttctctt
     2641 gatggtgtaa ctgatactgt aaaatatgat gtaaaaagtg acgcagaatc tacaggtatt
     2701 aacagcgttg gtttacatga tttacaacat agaatcgctt tacttgaagc tgaaaacgat
     2761 ggtcttaaag gggctgttaa ggctcgagat gagcatattg attcccttcg tcaggctatg
     2821 cagcttctgg agcacaagca cgaaccttct tcaccatccc attccccgtg gtggaaattc
     2881 tggaaaaaat cctgatcctg ccatgcttga gttttcctct ccggccatcc cgg
//
