LOCUS       CP125756                9294 bp    DNA     circular BCT 01-JUN-2023
DEFINITION  Klebsiella pneumoniae strain NU-CRE166 plasmid pNUCRE-166_6,
            complete sequence.
ACCESSION   CP125756
VERSION     CP125756.1
DBLINK      BioProject: PRJNA395086
            BioSample: SAMN17600204
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Cheung,B.H., Alisoltani,A., Kochan,T.J., Lebrun-Corbin,M.,
            Nozick,S.H., Bachta,K.E.R., Ozer,E.A. and Hauser,A.R.
  TITLE     Genome-wide screens reveal shared and strain-specific genes
            necessary for enteric colonization by Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Ozer,E.A., Lopez,F.K., Bulman,Z.P. and Hauser,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-2021) Medicine, Northwestern University Feinberg
            School of Medicine, 645 N Michigan Ave, Suite 900, Chicago, IL
            60611, USA
REFERENCE   3  (bases 1 to 9294)
  AUTHORS   Cheung,B.H., Kochan,T.J., Ozer,E.A. and Hauser,A.R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2023) Microbiology-Immunology, Northwestern
            University, 303 E Chicago Ave, Chicago, IL 60611, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/15/2023 16:16:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,987
            CDSs (total)                      :: 5,863
            Genes (coding)                    :: 5,715
            CDSs (with protein)               :: 5,715
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 65 of 148
            Pseudo Genes (incomplete)         :: 93 of 148
            Pseudo Genes (internal stop)      :: 18 of 148
            Pseudo Genes (multiple problems)  :: 25 of 148
            ##Genome-Annotation-Data-END##
            Bacteria and source DNA available from Alan Hauser (Northwestern
            University, 303 E Chicago Ave, Chicago, IL, USA).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="NU-CRE166"
                     /isolation_source="bronchoalveolar lavage"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pNUCRE-166_6"
                     /geo_loc_name="USA: Chicago, IL"
                     /lat_lon="41.8945 N 87.6208 W"
                     /collection_date="2014-02"
     gene            join(9133..9294,1..96)
                     /locus_tag="JNR65_029860"
     CDS             join(9133..9294,1..96)
                     /locus_tag="JNR65_029860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WIE26173.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            181..330
                     /locus_tag="JNR65_029865"
     CDS             181..330
                     /locus_tag="JNR65_029865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WIE26174.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1811..3775
                     /locus_tag="JNR65_029870"
     CDS             1811..3775
                     /locus_tag="JNR65_029870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WIE26175.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            3775..4506
                     /locus_tag="JNR65_029875"
     CDS             3775..4506
                     /locus_tag="JNR65_029875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="WIE26176.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="replication-relaxation family protein"
     gene            4513..5043
                     /locus_tag="JNR65_029880"
     CDS             4513..5043
                     /locus_tag="JNR65_029880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WIE26177.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5071..5250)
                     /locus_tag="JNR65_029885"
     CDS             complement(5071..5250)
                     /locus_tag="JNR65_029885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WIE26167.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5319..5696
                     /locus_tag="JNR65_029890"
     CDS             5319..5696
                     /locus_tag="JNR65_029890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WIE26168.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(5758..6057)
                     /locus_tag="JNR65_029895"
     CDS             complement(5758..6057)
                     /locus_tag="JNR65_029895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008657 - DNA topoisomerase type II (double strand cut,
                     ATP-hydrolyzing) inhibitor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization activity
                     [Evidence IEA]; GO_process: GO:0030541 - plasmid
                     partitioning [Evidence IEA]; GO_process: GO:0051290 -
                     protein heterotetramerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WIE26169.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6047..6310)
                     /locus_tag="JNR65_029900"
     CDS             complement(6047..6310)
                     /locus_tag="JNR65_029900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WIE26170.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(6616..7035)
                     /locus_tag="JNR65_029905"
     CDS             complement(6616..7035)
                     /locus_tag="JNR65_029905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WIE26171.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7438..9123
                     /locus_tag="JNR65_029910"
     CDS             7438..9123
                     /locus_tag="JNR65_029910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WIE26172.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             2438..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(434..1012)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3776..4503
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tgggtttcga tattacagcc acactttaaa aatgtaatcg atattagtaa atttgattac
       61 tttgtatcct ttgtttaccg ggatggtaac tggtaagcaa aagttatcaa ggatgatttg
      121 aaatacagac ttccggtgtt cagggatgaa taccggggtt tttaaataca gggaaggtat
      181 atgaaaaagg taaaggttgt ttttcttttt atattgattt tttcaggctt tcttctggtc
      241 gcatgtcagg caaactatat ccgggatgtt cagggtggaa cggtggcacc atcgtcctcc
      301 tctgaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt
      361 tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac cacctgggga ggtggtttga
      421 tcgaaggtta agtcagttgg ggaactgctt aaccgtggta actggctttc gcagagcaca
      481 gcaaccaaat ctgtccttcc agtgtagccg gactttggcg cacacttcaa gagcaaccgc
      541 gtgtttagct aaacaaatcc tctgcgaact cccagttacc aatggctgct gccagtggcg
      601 ttttgcgtgc ttttccgggt tggactcaag tgaacagtta ccggataagg cgcagcagtc
      661 gggctgaacg gggggttctt gcttacagcc cagcttggag cgaacgacct acaccgagcc
      721 gagataccag agtgtgagct atgagaaagc gccacacttc ccgcaaggga gaaaggcgga
      781 acaggtatcc gggaaacggc agggttggaa caggagagcg caagagggag cgattcatcg
      841 gaaacggtga tgatctttaa gtcctgtcgg gtttcgccac tcctgactga ttcatggttg
      901 agccaccggc tcccacagat gcaccgaaaa agcgtctgtt tatgtgaact cagtcaggag
      961 ggcggagcct atggaaaaac gccacctgcg tggccttgcc gttttgcctc acatgttagt
     1021 cccctgctta ttcacggaat ctgtgggtaa ctttgcatgt gtccgcagcg cccgccgcag
     1081 tctcacggcc ggagcgtagc gaccgagtga gcgaggaggc gcaatttccc cggcacctac
     1141 aagacggaca ccggacgcgc tgatgtggca tattgtcccc tgattaagtc tttatattcc
     1201 ctgataataa attaccagga taggtgaatc atatcggttt ttcgctgaac attttacgtt
     1261 aacctatctc tgttgctttt ttctccttac ggagaaatac aggtcatccc cgcgagggcg
     1321 tggctgacct cagggggata tcccctgctt acgctggctg gacgccaccg acgcacggtc
     1381 tctccccctc tttctggctc cgtccggctg gacgccggcc acgccagaaa ttcacccggc
     1441 ccccgtcagg ctcacgcctg accacccaca aaaatccacg gtgctggacg cacctggcga
     1501 agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc tcgctatggc gaactctgac
     1561 gggggccgga acaccggaaa agaccgcact ggggtgccgc cttttcctct cacacgctgg
     1621 ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca tggggcggaa agaggctggc
     1681 ctccgtggtg aaagctgaac gcaatcacca cggacgtgac cgccgccgcc atgccttggg
     1741 gcatgtccgc ggcttgtgca gcgggggctg cacatcacgc cggcaaacca tcgtctgaag
     1801 gagagagaac atgtttaata cggattcgct tgcctggaag tggcacattc cggagctggc
     1861 ccggcagatt tatccgtggg tcagctggct gtaccaggag tattacggtc tggccggggc
     1921 tttcctagtt atcggcctgt ttttcggtct ggctgaactt tgggccggac ggatgcgttt
     1981 tgtggggctg agtcataagg agcgacggga gtatgccgat cgctataagc gcgataataa
     2041 acggccagcg ccattttcgg tgctggcctc gctggattcg cccgggcgga tggtcagggc
     2101 acgtcgacat gtctggaact acgcgtactg gctgaccggc gcggccggtc tggcccttct
     2161 gggggggctg gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgtccgga
     2221 cctctacgca aacgcagatt attacctgct gcagccggct aacctgttcg ggattgcctg
     2281 gtgcgcgggc gggctggctg tcgggattgt gttggcgcgg ctgctggcgt tcctgctgct
     2341 tgacggtcat tttgcggctg ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg
     2401 gcgcagcagc cagcggacag gagagatgac cgacgtgcgc cacctgcatt tcggggagcc
     2461 ggtaccggtt aatgcgctgg ccgatttttc ctcggagcag gcccggcagg agcaggcggt
     2521 gtttctgggt aaggatgagc agggcaagcc ggtgctggtt ccgcgtgata cctggcgcaa
     2581 gaccaacatt cagatcctcg gcctgcccgg gagcggcaag agcgtgatgg ccaccaacgc
     2641 gctcattcgc tgcgtacgcg atttcgggga cgcggtggtg tacttcgacc ccaaagggga
     2701 cgcatgggcg ccgcacgtct tccgggcgca ctgtccggac ttcaccctgc tggatctgcg
     2761 gccggggaaa ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg cgctgaaaaa
     2821 cctgctgatt gccgggctta acctgggcga gaccggcgac gtggcggacc actaccgcat
     2881 cagcgagcag aaagcggcga agctgattgc ggagcagttt ccgcaggggg caaacattca
     2941 gcaggtgctg gcggcggcgt acgcgctgcc ggaggccctg aaaaaggacg tgaaggggtt
     3001 gatcactagg ctggaaaacg tcgctgacct gagcgtcctg cagacggaca gtggcatcga
     3061 tgtggcgggg attatcaacg gcggcggctg cctgtacgtt atcggctcga tggacgacga
     3121 ggcggtgatc cgtgtgcaaa aaatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg
     3181 ggacgagttc cgggagtggc cacacgtcag cgtgatgctc gatgaaatta aatatctgct
     3241 gtcgaagtat gtactgaacg cactggggac gctgcgcagc cgggactgca atctgttgct
     3301 cgcgcaccag tcgctgggcg atttcgggca gtgcgggcag gatctgcccg ctgaatttgt
     3361 gaaaaccacg gtactggata acacgccgat ccgctggttt taccgggcgg ccagcctgga
     3421 gtccgcgcag tgggcggcgg ggcagaccgg ggaaatcagg gtggacgtgg agcgccgccg
     3481 tgccagccgg gaggcgggga acgtggagca tatcagcggg gacagcttca tccagaagga
     3541 ggcgcggccg ctgtttgacg tgaacaccct gcaacacctg ccggacgggt tcgcggtcat
     3601 gaccgggctg ggtgtggccc ggatggcgtt cagcagtccg ctgcgggttg agcgccggga
     3661 aatcccgttg cagcgtttcc cggtgctggc gaaaaccgat cccctggcgg agttccggcc
     3721 cccggccgac ggcggcgggc cgaaagcgga tgatgatttt gcggggattt actgatggcg
     3781 ctggagctgt acaacgtcag ccacgcgaaa cgacaggcgc ggaacgcgga gaaaacccgg
     3841 ctgaccctgc gctggttgcg ggaagagctg tgttccacgg cggagctggt tgcgcggcgg
     3901 ctggggattg cggcggtgca gccggtctac cgcttcctcg acagcctggt ggcgaaaggg
     3961 ctgctggtcc ggaccaaata tccggtggac ggccgtcagg tcagcgtgtg ggggctgacg
     4021 ccgcatggcg tggcgttctc gttcgacgag gacgaaccgc tggcggatgt tattccgttt
     4081 cagccgtcaa gggtgtcggc agcacatctg ccgcaccggc tggccgtgca atccctgcgc
     4141 cttgatatgg aagccagagg ggccaccggc tggcgcattc tgcaccgtct gagtcttaaa
     4201 ggcatgaaag tgccggacgc cctggcggag ctggacggta aaacggtggc gtttgaggtg
     4261 gagcgcaccg tcaagagccg tcgccggtat caggaagtgg tgtccgggta tctgttcaac
     4321 cgcaaagcta acggcatcga tgaaatctgg tacatctgcc cggaccgggc gacgcaggtc
     4381 agggtgcagc gggcaatcct gtcggtggat gaaatcgtga acccacagac cggcgaggcc
     4441 cgcaaaacgg cagagctgga tcgtgaacgg ttgtttgcgt gctttaaatt tatgacaacg
     4501 gagtgaggga ttatggcgct gtacatggta tttattgaag acgcggagga ccggaacggc
     4561 gagcgctgca tcatgccgac cagcgagcat ctggaagata tggtggtgct cgcctggcag
     4621 accggggcgt atgttttctg ttttgtcgac cgggcccagc atctggcgat gatgaaatcc
     4681 atcgggatgt atgacgagcg gctcaacagc gacgaaaaag ccacgtactt tttcactggt
     4741 aaaaatccgc aatgggaacg gaacaaaccc acaggaatga agctgtgtga ttacctctat
     4801 cgcaaggtgg agcggttcgc catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc
     4861 tcaccggagc agattaacgc gatgattgac gtggtgagtg agcggcacgg ggaaagcgcg
     4921 gtgaacgaga agctgaaagc ggagctgatg cgggaatata acagccgcta ttttaacgtg
     4981 ccggtacctg aactgccgga ggacaaacct gcccgccggt ggtggccgtt cggcaaaaaa
     5041 taaatgccag caccggccat gccggtgctg ttattcctcc ccgagcttat cccgcatcac
     5101 ctgccagagt gtttccgcat cctcgtgtag ccgctcgcac atgtcggccg gggtatccat
     5161 atccagctca ttcagtttat cgagcaatag cagcgactgc tccttaataa atttagccat
     5221 attgagggct gttttttcct gttttgtcat gattctttcc ttccgggcat ggtgtgcgcc
     5281 tgtcaggtat actgtctcaa aaatagcggg gtgagacaat ggcgtgggtg acggtgagac
     5341 aggcgacgga actgacgggt aaagcccgaa gttccctgta tcgggatatg gcgaaaggcc
     5401 gcgtatcata ccgtacggaa gctgatggcg gccgggtggt ggataccagc gagcttatcc
     5461 gggtgtacgg tgagttaaga caggatgaga ctcacgagcg ggacgagttg agactgtcgg
     5521 gtgagactga aaaaatggcc tccgacgcgt tgatcgcgga gataaaagca ctcagggagg
     5581 aggtggccgg gctacggcag gaaatgcagt ccatgagact gctggaacac aaaccggaga
     5641 cagcagagac cccggccgga acaccgcgcc ggtggtggcc gtggggaaaa cgctgacgcg
     5701 ctgtgcttgt ccaaccggcg acggccacgc aatgaattgc gtccccgtca ccggttatta
     5761 gagaacaata tggggattct ggcgctggcc gaccacggcc agaacccgga cttttctggt
     5821 ctggtcatca acatcataga ggacatgctg tcccatcaga ctgatccggc gaacgtcctc
     5881 gccatagcgt gagtccactt catagagtcg cgggaagtcg gccagtttgt ccgccgcagc
     5941 gagaacccgg tcggtaacgt tctgcgcatc ccacagggat gaattttccg cgatatagtc
     6001 aaacagcgtc gttacatcct cgatggctcc ctgcttccat tccacgtcat acggcatcgc
     6061 gcagttcctg ataacggcca tgcaggcgct ggcggagttg ttccgtggtc atacccggac
     6121 gcgtgtcagc acgggcggcg gtcactttgg cgcgaacctg atcttcatac tctgcctggg
     6181 ccagttcttc gttagccagt cgctgcaaca gcgcagcttt gtccgggcca ccttccggca
     6241 gtatgcgggg caggctttca gccagttccc gaagaaagtt ggcgttttcg tagtgtgtag
     6301 tgttgctcat tgcggagcct cccgggttaa tgcggcaata tgaaggaagt ttagcagggg
     6361 gcggtctgaa agaatatggt cgtgtcagtc aggctgatga agcagaaagt cggtaaaaaa
     6421 gggcttacgg catccatttt acgtcaaaac atatgccatg cagttgcgtg cataggcatg
     6481 cattaggata aaatttatcg ggcgcgtttc cggcagtttt ccgggggggt tgttgcctgt
     6541 tttatcccgt agccgccgga aacgccctga gcccgtctga gcggtgcgcg taatgacgcg
     6601 ttatggtaaa tccacttact gtctgccttc atagccatcg agataaacag ctgaaatggt
     6661 gtcagccagt agccttgatt ttttcggtgt gaggtctttg ctgcggtaat cggtacccgc
     6721 aaattgtgtt gtgccttgga gtgagtttaa aaattcctga ctgtgaaact gctgaatgcg
     6781 ttgctgcaca tcactccgtg attttccggt cgctttatcc tttgcgccca tatcccagaa
     6841 ctgccggaaa aaaggcacca tcattcgtgt tgtgtcagct ttctgggtgg aaaaattggg
     6901 atcaaaaccg tcatctgaag catatacgta atgccgggta tgctgttcca cgctgccctt
     6961 tggagcacag gcagtgagag ttgctgtcgc tgttatcatg gcgataagac acagtttgct
     7021 gccggaacgt ctcatgagcg tctccttacc tttaattctt ttttctcatt gagttatata
     7081 tccgcaggct atcttatcat cagtttctga ccgtgttcag tgaccgaaca acccgaacca
     7141 gaccccccgt ttcttttcct gtcccggagg gggctttctg gtatctcata taatgatttc
     7201 agtaaaacat actgaggccg gagaacggcg ccgtttgtgc tggtttttgt tgcatctgga
     7261 ggggattgcg catggtgtga gtggctgttt ttttgatcat tttcacaaaa acgcaaattt
     7321 acagacttga catgaaaaac aaggcagcgt aatttttata ctgtatataa aaccagtggt
     7381 tatatgtaca gtattttatt ttagtttatt gttttaaaag tcaaagagga aaagtatatg
     7441 agtggcggag acggacgagg tccgggtaat tcaggtctgg gacacaatgg tggtcaggcc
     7501 agtgggaatg tgaacggtac atcaggtaaa ggtggccctt catcaggtgg tggtacggat
     7561 ccaaacagcg ggccgggctg gggtacgacg catacaccta acggggatat tcataactat
     7621 aacccggggg agtttggtaa cggcgggagt aaacccggtg gtaatggcgg taacagcggc
     7681 aatcatagcg ggagctctgg tggcggacag tcttcggcca ccgcgatggc cttcggtctg
     7741 cctgccttgg ccactccggg ggctgaagga ctggctttat ccgtttccgg cgatgcgttg
     7801 tcggccgctg ttgctgatgt gctggctgcc ctgaaaggtc cgtttaagtt tggtctgtgg
     7861 gggattgcga tttacggagt gctgccttct gagatagcaa aagatgatcc gaaaatgatg
     7921 tcaaaaatcg tgacgtcatt gccggcagac acggtaacgg agacgccggt cagctccctg
     7981 ccgctggacc aggcgacggt cagcgtcact aaacgtgtgg cggatattgt gaaggacgag
     8041 cggcagcata ttgcggttgt caccggccgg ccaatgagtg ttcctgtggt ggatgcgaaa
     8101 ccgacaaaac gtccgggggt attcagtgtg tcgattccgg gtctcccgtc tctgcaggtg
     8161 agcgtaccta aaggtgttcc ggccgcgaaa gccccgccaa aaggcattat tgctgaaaaa
     8221 ggtgattcac gtccggctgg ttttacggcc ggtggtaact cccgtgaagc cgttattcgt
     8281 ttcccgaaag agagcggaca gaaaccggtc tatgtgtcgg tgacggatgt tcttactccg
     8341 gcacaggtaa aacagcgtct ggaggaagaa aagcgtcgcc agcaggcatg ggacgctgct
     8401 cacccggaag aggggctgaa aagagagtat gataaagcga aagctgagct ggatgctgaa
     8461 gataaaaata ttgcgacctt aaacagtcgc attgcatcga cagagaaggc gatccccggt
     8521 gcaagggctg ctgttcagga agccgataaa aaggtaaaag aggcagaggc aaataaagat
     8581 gattttgtga cttataaccc gcctcatgaa tatggctccg ggtggcagga tcaggttcgc
     8641 tatcttgata aggatattca gaatcagaat gagaaattaa aagctgccca gacatcttta
     8701 aacgaaatga atgaatcctt atccagggat aaggctgcgc tttccggggc gatggagagc
     8761 cggaaacaaa aggagaaaaa agcgaaggat gcagaaaata aattaaatga ggaaaagaaa
     8821 aaacctcgta agggaactaa agattacggc catgattatt ttcctgatcc caagactgaa
     8881 gatattaagg ggttgggaga gttgaaagag ggtaaaccta aaacccctaa acaaggtggt
     8941 ggtggtaagc gggcccgatg gtatggtgat aaaaagcgta aaatttatga atgggattcc
     9001 cagcacggtg agcttgaagg gtaccgcgcc agtgatggcg aacacctcgg ggcattcgat
     9061 ccaaaaacgg gtaagcaggt taaagggccg gatccaaaac gaaacattaa aaaatatctt
     9121 taagaggtaa atatggggct taaattacat attcattggt ttgataagaa aaccgaagag
     9181 tttaaaggtg gtgaatactc aaaagacttc ggtgatgatg gttctgtcat tgaaagtctg
     9241 gggatgcctt taaaggataa tattaataat ggttggtttg atgttgaaaa atca
//
