LOCUS       CP125155               10226 bp    DNA     circular BCT 23-MAR-2024
DEFINITION  Klebsiella pneumoniae strain 176 plasmid unnamed1, complete
            sequence.
ACCESSION   CP125155
VERSION     CP125155.1
DBLINK      BioProject: PRJNA945360
            BioSample: SAMN33790397
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Konanov,D.N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAR-2023) Laboratory of Mathematical Biology and
            Bioinformatics, Research Institute for Systems Biology and Medicine,
            Nauchniy Proezd, Moscow 117246, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.8.1-b1676
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 80x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/09/2023 19:08:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,771
            CDSs (total)                      :: 5,646
            Genes (coding)                    :: 4,657
            CDSs (with protein)               :: 4,657
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 989
            CDSs (without protein)            :: 989
            Pseudo Genes (ambiguous residues) :: 0 of 989
            Pseudo Genes (frameshifted)       :: 897 of 989
            Pseudo Genes (incomplete)         :: 98 of 989
            Pseudo Genes (internal stop)      :: 22 of 989
            Pseudo Genes (multiple problems)  :: 26 of 989
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="176"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="Russia"
                     /collection_date="2022"
     gene            267..752
                     /locus_tag="P4G33_00040"
     CDS             267..752
                     /locus_tag="P4G33_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WXI97750.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNAKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            756..968
                     /locus_tag="P4G33_00045"
     CDS             756..968
                     /locus_tag="P4G33_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WXI97751.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1476..1934)
                     /locus_tag="P4G33_00050"
     CDS             complement(1476..1934)
                     /locus_tag="P4G33_00050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXI97752.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            3486..4280
                     /locus_tag="P4G33_00055"
                     /pseudo=""
     CDS             3486..4280
                     /locus_tag="P4G33_00055"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4318..4803
                     /locus_tag="P4G33_00060"
     CDS             4318..4803
                     /locus_tag="P4G33_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WXI97753.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNAKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            4807..5019
                     /locus_tag="P4G33_00065"
     CDS             4807..5019
                     /locus_tag="P4G33_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WXI97754.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5527..5985)
                     /locus_tag="P4G33_00070"
     CDS             complement(5527..5985)
                     /locus_tag="P4G33_00070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXI97746.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            7381..8550
                     /locus_tag="P4G33_00075"
     CDS             7381..8550
                     /locus_tag="P4G33_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WXI97747.1"
                     /translation="MWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPARACRLPSISPA
                     LLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGMMIVK
                     FHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVRELIDASPYAKKYTSGVLSFAEA
                     ELLPGQREQIMASFERVLMPGLDKDQYSILWVEHTDKGRLELNFLIPNTELLTGKRLQP
                     YYDRADRPRIDAWQTVVNGLADFLDLNTGNDKEIVADYGLGRNCTLFDKTRKWAYRAIR
                     QGWPEYEQWLQACYERACAYNLQFARPLDENEVRGIAKSIAKWTSNKFSPEEFSKFVDI
                     THSSEIQSKRGKKSGQSRRKGSLEEIKPWVAMGISRRKYFYIKKNGEIR"
                     /name="primase C-terminal domain-containing protein"
     gene            8547..9254
                     /locus_tag="P4G33_00080"
     CDS             8547..9254
                     /locus_tag="P4G33_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WXI97748.1"
                     /translation="MKTFVTLWTIVFLAFIAVMCTVDISNYIPSVYTVFSKNRPLVTGA
                     LSILLISVFIWLMVSLYLLKKYSVKVEKLSLGGVNILFNESATLYKKSVKNHLDSKRAI
                     FKLRKNVDAFDEVISSYYQTYQFIRDEMKILNPKKDREIYDFSNEMLMVLNRFLTNYQN
                     NYKRWYKYISDKDEVVDRVSGDVMRFHATSINKIQMQYYDYERILKGFKDVNRFFVNDV
                     KPKFGINTDKWNW"
                     /name="hypothetical protein"
     gene            complement(9291..9905)
                     /locus_tag="P4G33_00085"
     CDS             complement(9291..9905)
                     /locus_tag="P4G33_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="WXI97749.1"
                     /translation="MGKHKVFISYHHANDQYYKNALEKMNEEHEIFVNRSVSLGDIDED
                     EAPQKIREIIRDEYLRDTSVLILLVGTETKNRKHVDWELYSSMRDSTINKKSGILVVNL
                     PSTNTTYIRSTHGTNEKSEVHPTVTEWFSIDKRSTYEERFPYMPARIIDNFMKSGSYIS
                     VVNWNQIENNPETLRKLIDMTYNDREKATYDMSREMRMHNS"
                     /name="TIR domain-containing protein"
     Typing          3034..3213
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          7085..7264
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          complement(2571..3051)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          complement(6622..7102)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          3..1084
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.23"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3322..3623
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.196"
                     /coverage="95.0"
                     /evalue="1.64e-54"
     Typing          3631..5135
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.024"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7373..7674
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.196"
                     /coverage="95.0"
                     /evalue="3.11e-54"
     Typing          7670..8127
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="93.0"
                     /evalue="1.48e-78"
ORIGIN
        1 cagactggga ctgcatgatc caaacgcccc ggagaaccgg cggttactgg ttacgccgtc
       61 cgcactgccg gaaacgaagc tggaagccgc tcaggcgatt acacgtgggt tgctggcctt
      121 agcctcatcc ggggagctca aaactcgcga ggacgtcaca ggtgcgctca cagcggcagg
      181 ttttgaggtg gtccgcacta caaaaaacag tatcagcatt gccgacccgg acggggggcg
      241 aaacatccga ctgaagggag ccatttatga gcagtctttt agcgctggcg aaggacttgg
      301 agcagcaatc gaaagcgcag cagcagagta ccggcgagat gctgaaagcc gcattcagcg
      361 agcacgagaa atctgtaaaa gcggaactga gcgcaagcgc gaagagaatc agcgacgcca
      421 tcagcgccca cgagcagggt atgaccgcgg cgatgcagtc gaaccgcctg agcgtgctgc
      481 gtatggtcgg ccggacgtgg ctgaccatca ccctggtctc ggtactgctg atcgccatga
      541 gcgggagcat tctgtggtgg caggggcagc agatcaccgg gaattaccag actatccggg
      601 cgcaggagcg gacgcaggcc atgttgtcgg agaaaaacca cggcgttcag ctgtcgctct
      661 gcggggagaa gaaactgtcc tgcgtcaaag tgaacgcgaa agcgggagct tacggggagg
      721 aagggaactg gatggtactg gagaggaaat agcccatgac ggaactggaa aaacagttgc
      781 tgaccgcatt agagcagcta catcaggact actcgcaaag gctggacgag tgggagagcg
      841 ccttcgcgga atggcagaag atgtctggtc ttatgcaacg ggagaacgcg gcgctgaacg
      901 agcgcgtcac gcgcttgagt cagcaggtgg cgaacttgag caggcagtta cagcgtttga
      961 gccagtagtg cagcggcatg aaatggcggt ggccgccgaa cgggcatacg agcagcgaca
     1021 gcacgaaaaa gagctggcgg aagcccgttc ccgtcagcgg agctataacg gtccgacgct
     1081 gggctgacag gcttcaccag tgacaaaccc cacgaagaag ggctcataaa tttcccgcta
     1141 aatacggtcg tgatgaaaac atcacggcgt atacaacgga gggattatga gactgtttaa
     1201 cgtgattgag acggtgctga actgcctgta catggcgctg gagatctggg actggctgga
     1261 gaagcatttt cctgttttta aggcgttcat ccagattctg gcctgaaatc agaccgtatg
     1321 catctggctg catgaccatg cagccgggta aaatttatct gtcacgaacg gggcggtgta
     1381 tcgggtggtt tgttgccggt tttacctgac tgccgccccg tttgtgacga acatgtccgg
     1441 agcggtgcgg gcaagagatg ttatggtaaa tcatattagt tctgaatgat tcgggctcca
     1501 ccagtcatta ctatttgagc accacctgac atttttatac ttgtaccctg gctagtcatt
     1561 gtcgtatcac cttgtatgat cgtttttcca gtagcatgtt gacctccacc agtgactaaa
     1621 attgacgctt gtacttgaga tccatttatc aaaacagttc cggcttggtg atcaaatttt
     1681 attccgctca atgcttttaa ttcagctcca tctagctgaa tttcaccatt ggctgctgca
     1741 gcctgaatgg ccccctgcat ctgagaaaat aatttatcaa cttcgtcaaa aggtaatggt
     1801 gtttcaagca gtgaggcttg ttgttcaact tcaggatgtt tacttgctaa cttccctatg
     1861 atgcctttga ttgcatcaga agtaagattg cttaatgcgc cagtgagaac ggcaagtagt
     1921 gtggtttcaa tcattatatt tccctcgtta gattgctgtg atgcgtatta tttactcttt
     1981 ataatctccg attctgtcca tatggttagt tgcactatta cgtttataag tgccactttt
     2041 accatatata tatccaacgc tacattgtgg acattttggc gtctcgtcat tatgagtgaa
     2101 atttatttct cctccgacag atagggtttt tacgttttcc ggataatcta atgtagccac
     2161 gccggtccat tcacaattcg agcagacaac atcgaaagat gttgggatac tcttgcagtt
     2221 gatgttgatt tgcatatgca taacctcgat tttgcacaga aattacagtg ttattagttg
     2281 cataccgact ataacctgag tttgtgatgg agtggtgttt ttttgctttg acttgagtaa
     2341 gagaaagaga tgatcttctt gagataatct ctttctgagc gtagtctttt gctctgtaaa
     2401 cgaaaaaacc accctggcag gtggtttttc gaaggttagt taatcctggc agatttacta
     2461 accgtggtaa cagtcttgtg cgagacatgt caccaaagct gtccttccag tgtagccgca
     2521 gtaaggccac cacttcaaga actctgaata catctctcgc acatcctgtt taccagtggc
     2581 tactgccaat ggcgatttgt cgtgcctttt cgggttggac tcaagacgat agttaccgaa
     2641 aagagcgtgg tagtcggact gaacgggggg atcgtgcata cagtccagct tggagcgaac
     2701 tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa
     2761 tgacaccggt aaaccgaatg gcaggaacag gagagcgcac gagggagcta ccagggggaa
     2821 acgcctggga tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt
     2881 tatgatactc gtcagggggg cggagcctat ggaaaaacgg caacgccgca tcctcttccc
     2941 ggcttctcct gatttgagcg ctgcttgcgt atcccgtcat ctgtgagctg gtaacgcccg
     3001 ccgcagtctc acggcaggag cgcagcgact gagtgagcga ggaggcgtca tttcaccttt
     3061 accagcatgt tgcacttacg cccgtcagcc acattttccc cttcccacat gaagcacttt
     3121 catacattac cactccgccg taacatattt agccagtata cactccgcta gcgctacgtg
     3181 actggttcag ggctgcgccc cgaaacccgc taaaagccac tgacgcgcct gcggcttgtc
     3241 caacaccgtg ccgaccggga aacatttccc gcccgtcccg gtgttatcag gaggctactc
     3301 tggcagacaa acgcaacaag atgctcacga tgtgggtgac ggaagacgaa caccggcggc
     3361 tgctcgagcg gtgcgacggc aggcagctgg cggcgtggat gcgtcagtcc tgcctcgatg
     3421 agaagccggc gcgtgcctgc agactgccgt caatatcgcc ggcgctgctg cgccagcttg
     3481 ccggtatggg taacaacctg aaccagatcg cccggaaagt gaatgccgtt ggtgccggtc
     3541 atgatcgggt gcagattgtg gccgccctga tggcaataga cgcgggactg gagcggctgc
     3601 ggcatgcggt gctggaaaaa ggggggatga tgatcgttaa atttcatccc cgggggcgcg
     3661 gtggtggtgc cggtccggtg gattacctgc tgggaaaaga ccggcagcgc gagggggcga
     3721 ccgtgctgca gggaaaaccg gaggaagtcc gggagcttat cgatgcctcg ccgtatgcga
     3781 aaaagtacac ctccggcgtg ctgtcgttcg cggaggctga actgctgccc gggcagcggg
     3841 agcagatcat ggccagcttt gagcgggttc tgatgcccgg gctcgataaa gaccagtaca
     3901 gcattctgtg ggtggagcac accgacaagg ggcggctgga gctgaatttc ctgatcccca
     3961 acactgaact gctgaccggg aaacgcctgc agccgtatta cgatcgcgcc gaccggccgc
     4021 gcatcgatgc ctggcagacc gtggtgaacg gcagactggg actgcatgat ccaaacgccc
     4081 cggagaaccg gcggttactg gttacgccgt ccgcactgcc ggaaacgaag ctggaagccg
     4141 ctcaggcgat tacacgtggg ttgctggcct tagcctcatc cggggagctc aaaactcgcg
     4201 aggacgtcac aggtgcgctc acagcggcag gttttgaggt ggtccgcact acaaaaaaca
     4261 gtatcagcat tgccgacccg gacggggggc gaaacatccg actgaaggga gccatttatg
     4321 agcagtcttt tagcgctggc gaaggacttg gagcagcaat cgaaagcgca gcagcagagt
     4381 accggcgaga tgctgaaagc cgcattcagc gagcacgaga aatctgtaaa agcggaactg
     4441 agcgcaagcg cgaagagaat cagcgacgcc atcagcgccc acgagcaggg tatgaccgcg
     4501 gcgatgcagt cgaaccgcct gagcgtgctg cgtatggtcg gccggacgtg gctgaccatc
     4561 accctggtct cggtactgct gatcgccatg agcgggagca ttctgtggtg gcaggggcag
     4621 cagatcaccg ggaattacca gactatccgg gcgcaggagc ggacgcaggc catgttgtcg
     4681 gagaaaaacc acggcgttca gctgtcgctc tgcggggaga agaaactgtc ctgcgtcaaa
     4741 gtgaacgcga aagcgggagc ttacggggag gaagggaact ggatggtact ggagaggaaa
     4801 tagcccatga cggaactgga aaaacagttg ctgaccgcat tagagcagct acatcaggac
     4861 tactcgcaaa ggctggacga gtgggagagc gccttcgcgg aatggcagaa gatgtctggt
     4921 cttatgcaac gggagaacgc ggcgctgaac gagcgcgtca cgcgcttgag tcagcaggtg
     4981 gcgaacttga gcaggcagtt acagcgtttg agccagtagt gcagcggcat gaaatggcgg
     5041 tggccgccga acgggcatac gagcagcgac agcacgaaaa agagctggcg gaagcccgtt
     5101 cccgtcagcg gagctataac ggtccgacgc tgggctgaca ggcttcacca gtgacaaacc
     5161 ccacgaagaa gggctcataa atttcccgct aaatacggtc gtgatgaaaa catcacggcg
     5221 tatacaacgg agggattatg agactgttta acgtgattga gacggtgctg aactgcctgt
     5281 acatggcgct ggagatctgg gactggctgg agaagcattt tcctgttttt aaggcgttca
     5341 tccagattct ggcctgaaat cagaccgtat gcatctggct gcatgaccat gcagccgggt
     5401 aaaatttatc tgtcacgaac ggggcggtgt atcgggtggt ttgttgccgg ttttacctga
     5461 ctgccgcccc gtttgtgacg aacatgtccg gagcggtgcg ggcaagagat gttatggtaa
     5521 atcatattag ttctgaatga ttcgggctcc accagtcatt actatttgag caccacctga
     5581 catttttata cttgtaccct ggctagtcat tgtcgtatca ccttgtatga tcgtttttcc
     5641 agtagcatgt tgacctccac cagtgactaa aattgacgct tgtacttgag atccatttat
     5701 caaaacagtt ccggcttggt gatcaaattt tattccgctc aatgctttta attcagctcc
     5761 atctagctga atttcaccat tggctgctgc agcctgaatg gccccctgca tctgagaaaa
     5821 taatttatca acttcgtcaa aaggtaatgg tgtttcaagc agtgaggctt gttgttcaac
     5881 ttcaggatgt ttacttgcta acttccctat gatgcctttg attgcatcag aagtaagatt
     5941 gcttaatgcg ccagtgagaa cggcaagtag tgtggtttca atcattatat ttccctcgtt
     6001 agattgctgt gatgcgtatt atttactctt tataatctcc gattctgtcc atatggttag
     6061 ttgcactatt acgtttataa gtgccacttt taccatatat atatccaacg ctacattgtg
     6121 gacattttgg cgtctcgtca ttatgagtga aatttatttc tcctccgaca gatagggttt
     6181 ttacgttttc cggataatct aatgtagcca cgccggtcca ttcacaattc gagcagacaa
     6241 catcgaaaga tgttgggata ctcttgcagt tgatgttgat ttgcatatgc ataacctcga
     6301 ttttgcacag aaattacagt gttattagtt gcataccgac tataacctga gtttgtgatg
     6361 gagtggtgtt tttttgcttt gacttgagta agagaaagag atgatcttct tgagataatc
     6421 tctttctgag cgtagtcttt tgctctgtaa acgaaaaaac caccctggca ggtggttttt
     6481 cgaaggttag ttaatcctgg cagatttact aaccgtggta acagtcttgt gcgagacatg
     6541 tcaccaaagc tgtccttcca gtgtagccgc agtaaggcca ccacttcaag aactctgaat
     6601 acatctctcg cacatcctgt ttaccagtgg ctactgccaa tggcgatttg tcgtgccttt
     6661 tcgggttgga ctcaagacga tagttaccga aaagagcgtg gtagtcggac tgaacggggg
     6721 gatcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     6781 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaat ggcaggaaca
     6841 ggagagcgca cgagggagct accaggggga aacgcctggg atctttatag tcctgtcggg
     6901 tttcgccacc actgatttga gcgtcagatt ttatgatact cgtcaggggg gcggagccta
     6961 tggaaaaacg gcaacgccgc atcctcttcc cggcttctcc tgatttgagc gctgcttgcg
     7021 tatcccgtca tctgtgagct ggtaacgccc gccgcagtct cacggcagga gcgcagcgac
     7081 tgagtgagcg aggaggcgtc atttcacctt taccagcatg ttgcacttac gcccgtcagc
     7141 cacattttcc ccttcccaca tgaagcactt tcatacatta ccactccgcc gtaacatatt
     7201 tagccagtat acactccgct agcgctacgt gactggttca gggctgcgcc ccgaaacccg
     7261 ctaaaagcca ctgacgcgcc tgcggcttgt ccaacaccgt gccgaccggg aaacatttcc
     7321 cgcccgtccc ggtgttatca ggaggctact ctggcagaca aacgcaacaa gatgctcacg
     7381 atgtgggtga cggaagacga acaccggcgg ctgctcgagc ggtgcgacgg caggcagctg
     7441 gcggcgtgga tgcgtcagtc ctgcctcgat gagaagccgg cgcgtgcctg cagactgccg
     7501 tcaatatcgc cggcgctgct gcgccagctt gccggtatgg gtaacaacct gaaccagatc
     7561 gcccggaaag tgaatgccgt tggtgccggt catgatcggg tgcagattgt ggccgccctg
     7621 atggcaatag acgcgggact ggagcggctg cggcatgcgg tgctggaaaa aggggggatg
     7681 atgatcgtta aatttcatcc ccgggggcgc ggtggtggtg ccggtccggt ggattacctg
     7741 ctgggaaaag accggcagcg cgagggggcg accgtgctgc agggaaaacc ggaggaagtc
     7801 cgggagctta tcgatgcctc gccgtatgcg aaaaagtaca cctccggcgt gctgtcgttc
     7861 gcggaggctg aactgctgcc cgggcagcgg gagcagatca tggccagctt tgagcgggtt
     7921 ctgatgcccg ggctcgataa agaccagtac agcattctgt gggtggagca caccgacaag
     7981 gggcggctgg agctgaattt cctgatcccc aacactgaac tgctgaccgg gaaacgcctg
     8041 cagccgtatt acgatcgcgc cgaccggccg cgcatcgatg cctggcagac cgtggtgaac
     8101 ggcctggcag actttctcga tctgaacaca gggaatgaca aggaaatcgt tgccgactac
     8161 ggcttaggcc gtaactgcac gttgttcgac aaaacccgta agtgggcata tcgcgccatc
     8221 cgtcagggct ggcctgagta tgagcagtgg ctgcaagcct gctacgaacg cgcttgcgca
     8281 tacaacctgc aatttgcacg gcctctggat gaaaatgagg taagagggat agctaagagc
     8341 attgctaaat ggacatcaaa taaattttca cctgaagaat tcagtaaatt tgttgatatt
     8401 actcattcct ctgaaattca atcaaagaga gggaaaaagt caggacagtc aaggaggaaa
     8461 ggaagcctag aggaaataaa accttgggta gctatgggaa taagcaggcg gaagtatttt
     8521 tatatcaaga aaaatggtga aataagatga aaaccttcgt aactctttgg actatagttt
     8581 ttttagcatt tattgccgtg atgtgtactg ttgatatatc taattacatt ccgtctgttt
     8641 acacagtttt tagtaagaat agacctcttg taactggcgc cctttctatt cttttgatat
     8701 ctgtttttat ttggctgatg gtatccctct acttacttaa aaagtattcg gttaaggttg
     8761 agaaacttag ccttggtgga gtgaatatac ttttcaatga gagtgctaca ctttacaaaa
     8821 agagtgtaaa aaatcaccta gattcaaaaa gagcaatttt caaattaaga aaaaacgttg
     8881 atgcctttga tgaggttatt tcatcatatt atcagactta ccaatttatt cgtgatgaaa
     8941 tgaaaatact aaaccctaaa aaagatcgtg agatttatga tttcagcaat gagatgctca
     9001 tggtgttaaa tagatttcta actaactacc agaacaacta caagcgttgg tataagtaca
     9061 tatcagacaa agatgaggtt gttgataggg ttagcggtga tgtcatgaga tttcatgcaa
     9121 ccagcataaa taaaatacaa atgcagtatt atgattatga gcgtatttta aaaggtttta
     9181 aagatgttaa ccggttcttt gttaatgatg tgaagcctaa gtttggaatc aatacagata
     9241 agtggaactg gtaaattaaa ggagtagcca aggctactcc ttggatagta ctatgaatta
     9301 tgcattctca tttctctcga catatcatac gttgcttttt ctctatcgtt ataagtcatg
     9361 tctattaatt ttctgagtgt ttctgggttg ttttctattt gattccagtt gaccactgat
     9421 atataagacc cgctcttcat gaagttgtct attattcttg ctggcatata tgggaacctt
     9481 tcctcataag ttgagcgctt atcaatagaa aaccattcag ttacggtagg atgaacttct
     9541 gatttttcat tagttccatg tgttgaacga atgtacgtag tatttgttga cgggagattg
     9601 acgactaaaa ttcctgattt tttgttaatg gtactatctc tcatgctaga gtaaagttcc
     9661 caatctacgt gttttctgtt ttttgtttca gttccaacaa gaaggataag aactgatgta
     9721 tctctaagat attcatctct tattatctct cttatttttt gtggggcttc atcttcatct
     9781 atatcaccta atgatactga gcgattcacg aatatttcat gttcctcatt cattttttca
     9841 agtgcgtttt tgtagtactg atcattcgca tggtggtagc taatgaatac cttatgcttt
     9901 cccattttta ttgtcctttt tttgtttgtg tgcactctat tttgcacttg aacctatatc
     9961 agataacagc tctcttttag agagctcttt tttctcagcc agcacacata gggtcccttc
    10021 agcaaggtct cgccatgact ggtttagggc gggctcgttg cttccctaaa cgccttgaga
    10081 agagagctat cacctcactt tattcttacg taccgctcgc cgcagtctta tgaccgaacg
    10141 tagtgagcga atgcgcgagg aagcggaaag tcatttgttg cctgcatttt gcacggtcac
    10201 tcctgtgttt catcgccctt ctgaaa
//
