LOCUS       CP125070                2113 bp    DNA     circular BCT 16-MAY-2023
DEFINITION  Escherichia coli strain KFS-B24 plasmid pKFS-B24_6, complete
            sequence.
ACCESSION   CP125070
VERSION     CP125070.1
DBLINK      BioProject: PRJNA956323
            BioSample: SAMN34598740
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Gomi,R., Matsumura,Y., Yamamoto,M., Matsuda,T. and Harada,H.
  TITLE     Genomic surveillance of antimicrobial-resistant Escherichia coli in
            fecal sludge and sewage in Uganda
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Gomi,R., Matsumura,Y., Yamamoto,M., Matsuda,T. and Harada,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/09/2023 16:46:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,120
            CDSs (total)                      :: 5,003
            Genes (coding)                    :: 4,789
            CDSs (with protein)               :: 4,789
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 214
            CDSs (without protein)            :: 214
            Pseudo Genes (ambiguous residues) :: 0 of 214
            Pseudo Genes (frameshifted)       :: 81 of 214
            Pseudo Genes (incomplete)         :: 148 of 214
            Pseudo Genes (internal stop)      :: 39 of 214
            Pseudo Genes (multiple problems)  :: 50 of 214
            Pseudo Genes (short protein)      :: 4 of 214
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KFS-B24"
                     /isolation_source="Fecal sludge"
                     /db_xref="taxon:562"
                     /plasmid="pKFS-B24_6"
                     /geo_loc_name="Uganda"
                     /lat_lon="0.19 N 32.35 E"
                     /collection_date="2018"
                     /collected_by="Hidenori Harada, Mai Tanaka"
     gene            203..1168
                     /locus_tag="QL139_25595"
     CDS             203..1168
                     /locus_tag="QL139_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WHK00779.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1172..1438
                     /locus_tag="QL139_25600"
     CDS             1172..1438
                     /locus_tag="QL139_25600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHK00778.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          597..828
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gagtgggttt tgacaggtca aagaaaatgg agcagaattg aggcgttttt aatcggcgtt
       61 ggggagtgcg tcaacactcc ccaacatttc gaatgtgtca cctcagcggc aaactctggt
      121 gacatgtact ggctcgcaat gcacaggtac gtgatgaata taccacatca aatcacagcc
      181 tgcccagatc ggagcaggct taatgtcaga agataaattc ctttcggact acagcccccg
      241 tgatgcagtt tgggataccc agcgcacgct taccgattct gtcgggggta tctaccagac
      301 tgctgctgaa ttcgagcgct atgcactccg tatggcctcc tgtagcggtt tgttacgttt
      361 tggttggtct accatcatgg aaaccggaga aacgcgccta cggcttcgta gtgcgcaatt
      421 ttgccgtgtc cgtcattgcc ctgtctgcca gtggagaaga accctcatgt ggcaagcccg
      481 tttttatcag gctctaccga aaatcgttgt ggattacccg tcttcccgat ggttgtttct
      541 gacgttaact gtcaggaact gcgagatagg tgaacttgga acagtcctta cagcaatgaa
      601 tgcggcgttt aagcgaatgg aaaagcgaaa ggagctatca cctgttcagg ggtggatcag
      661 ggctacggag gtgacgcgag gtaaggatgg cagcgcacat ccgcattttc actgtctgct
      721 gatggtgcaa ccttcttggt ttaaagggaa gaactacgtt aagcacgaac gttgggtaga
      781 actctggcgc gattgcttgc gggtgaacta tgagccgaat atcgatattc gggcagtaaa
      841 aactaagaca ggtgaggttg tggccaacgt tgccgagcaa ctgcaaagcg cggttgctga
      901 aacgctgaaa tactccgtta aaccggaaga tatggcaaac gatcctgagt ggtttcttga
      961 gctgacgcgg cagcttcaca agcgccgttt tatctcgacc ggtggggcgc taaaaaacgt
     1021 cctccagttg gatcgagaaa ccaatgagga tcttgtcatt gccgacgatg taggggatgg
     1081 cactgatgac gggaagcgga cggcgtttgt ctgggattca ggtaaacggc gttacaaacg
     1141 cgcccctgag aaggataaat cggattaacg tatgaatatt aatattgaat acctgaatgg
     1201 aaataagact attggtttat tttttttaag aagtgaagcg gtgattcctg acaggtttaa
     1261 aaaccttatt ttgcttattg atggattaag ttttggcaca tttggttttc atccgcacga
     1321 aggttttgag gatgaattaa ttttatatat tcagaaaaca aacgagaggg taaaaactct
     1381 ttttgtgaaa atagatttaa acggtatcaa gtttggattt ttaagaacgc attcttagtt
     1441 ctggaaaaga gccagcggca ggctgaggtg ataggtacga gattgcatgc aatctctagt
     1501 gctctgtcta tcctgcatta tcctcagcat tatcctcagc attatcctca gcattatcct
     1561 cagccttgcc aactcgacac caatgcagga tagacaatcc gatgtcaaat gttaacactc
     1621 tgcgagtggt acattttccc cggattatcg tcctgagcct gccgctggct ctctttctac
     1681 cgcctcgctt tgctcgttgc tcaacgcctc acagacacgg attaaaatcc gcatccgttc
     1741 accgtttttt aaagtccgtt aaaagcatga tgccatctcc gagagttaat ctcgtcaaat
     1801 gctaaatcgt gggggtcccc tttggggttc cgatttagtg attgacgaca ccaccgatta
     1861 aaaaacttat gcggggtgga tggtttcacg aagtgaggcc atccacctgt aagacagggt
     1921 tttgttttta ttccctgttt tggtgatcgg gtgtgtggaa aaggttgggg taagccgttc
     1981 gggggtgctt gttttggggg gttaaaattg tggttatttt ttgcgcaatt ctcgcgcgtg
     2041 atccttgtat ttatacttaa gggataaatg gcggatatga aatagtggtt tagcccagta
     2101 atgacgaggc ttt
//
