LOCUS       CP125058                2101 bp    DNA     circular BCT 16-MAY-2023
DEFINITION  Escherichia coli strain KFS-C08 plasmid pKFS-C08_5, complete
            sequence.
ACCESSION   CP125058
VERSION     CP125058.1
DBLINK      BioProject: PRJNA956323
            BioSample: SAMN34598742
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Gomi,R., Matsumura,Y., Yamamoto,M., Matsuda,T. and Harada,H.
  TITLE     Genomic surveillance of antimicrobial-resistant Escherichia coli in
            fecal sludge and sewage in Uganda
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Gomi,R., Matsumura,Y., Yamamoto,M., Matsuda,T. and Harada,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/09/2023 16:42:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,967
            CDSs (total)                      :: 4,850
            Genes (coding)                    :: 4,635
            CDSs (with protein)               :: 4,635
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 83 of 215
            Pseudo Genes (incomplete)         :: 146 of 215
            Pseudo Genes (internal stop)      :: 34 of 215
            Pseudo Genes (multiple problems)  :: 45 of 215
            Pseudo Genes (short protein)      :: 4 of 215
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KFS-C08"
                     /isolation_source="Fecal sludge"
                     /db_xref="taxon:562"
                     /plasmid="pKFS-C08_5"
                     /geo_loc_name="Uganda"
                     /lat_lon="0.19 N 32.35 E"
                     /collection_date="2018"
                     /collected_by="Hidenori Harada, Mai Tanaka"
     gene            join(1912..2101,1..776)
                     /locus_tag="QL145_24830"
     CDS             join(1912..2101,1..776)
                     /locus_tag="QL145_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WHJ91537.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            780..1046
                     /locus_tag="QL145_24835"
     CDS             780..1046
                     /locus_tag="QL145_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHJ91538.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          205..436
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 cgcgcctacg gcttcgtagt gcgcaatttt gccgtgtccg tcattgccct gtctgccagt
       61 ggagaagaac cctcatgtgg caagcccgtt tttatcaggc tctaccgaaa atcgttgtgg
      121 attacccgtc ttcccgatgg ttgtttctga cgttaactgt caggaactgc gagataggtg
      181 aacttggaac agtccttaca gcaatgaatg cggcgtttaa gcgaatggaa aagcgaaagg
      241 agctatcacc tgttcagggg tggatcaggg ctacggaggt gacgcgaggt aaggatggca
      301 gcgcacatcc gcattttcac tgtctgctga tggtgcaacc ttcttggttt aaagggaaga
      361 actacgttaa gcacgaacgt tgggtagaac tctggcgcga ttgcttgcgg gtgaactatg
      421 agccgaatat cgatattcgg gcagtaaaaa ctaagacagg tgaggttgtg gccaacgttg
      481 ccgagcaact gcaaagcgcg gttgctgaaa cgctgaaata ctccgttaaa ccggaagata
      541 tggcaaacga tcctgagtgg tttcttgagc tgacgcggca gcttcacaag cgccgtttta
      601 tctcgaccgg tggggcgcta aaaaacgtcc tccagttgga tcgagaaacc aatgaggatc
      661 ttgtcattgc cgacgatgta ggggatggca ctgatgacgg gaagcggacg gcgtttgtct
      721 gggattcagg taaacggcgt tacaaacgcg cccctgagaa ggataaatcg gattaacgta
      781 tgaatattaa tattgaatac ctgaatggaa ataagactat tggtttattt tttttaagaa
      841 gtgaagcggt gattcctgac aggtttaaaa accttatttt gcttattgat ggattaagtt
      901 ttggcacatt tggttttcat ccgcacgaag gttttgagga tgaattaatt ttatatattc
      961 agaaaacaaa cgagagggta aaaactcttt ttgtgaaaat agatttaaac ggtatcaagt
     1021 ttggattttt aagaacgcat tcttagttct ggaaaagagc cagcggcagg ctgaggtgat
     1081 aggtacgaga ttgcatgcaa tctctagtgc tctgtctatc ctgcattatc ctcagcatta
     1141 tcctcagcat tatcctcagc cttgccaact cgacaccaat gcaggataga caatccgatg
     1201 tcaaatgtta acactctgcg agtggtatat tttccccgga ttatcgtcct gagcctgccg
     1261 ctggctctct ttctaccgcc tcgctttgct cgttgctcaa cgcctcacag acacggatta
     1321 aaatccgcat ccgttcaccg ttttttaaag tccgttaaaa gcatgatgcc atctccgaga
     1381 gttaatctcg tcaaatgcta aatcgtgggg gtcccctttg gggttccgat ttagtgattg
     1441 acgacaccac cgattaaaaa acttatgcgg ggtggatggt ttcacgaagt gaggccatcc
     1501 acctgtaaga cagggttttg tttttattcc ctgttttggt gatcgggtgt gtggaaaagg
     1561 ttggggtaag ccgttcgggg gtgcttgttt tggggggtta aaattgtggt tattttttgc
     1621 gcaattctcg cgcgtgatcc ttgtatttat acttaaggga taaatggcgg atatgaaata
     1681 gtggtttagc ccagtaatga cgaggctttg agtgggtttt gacaggtcaa agaaaatgga
     1741 gcagaattga ggcgttttta atcggcgttg gggagtgcgt caacactccc caacatttcg
     1801 aatgtgtcac ctcagcggca aactctggtg acatgtactg gctcgcaatg cacaggtacg
     1861 tgatgaatat accacatcaa atcacagcct gcccagatcg gagcaggctt aatgtcagaa
     1921 gataaattcc tttcggacta cagcccccgt gatgcagttt gggataccca gcgcacgctt
     1981 accgattctg tcgggggtat ctaccagact gctgctgaat tcgagcgcta tgcactccgt
     2041 atggcctcct gtagcggttt gttacgtttt ggttggtcta ccatcatgga aaccggagaa
     2101 a
//
