LOCUS       CP125052                2089 bp    DNA     circular BCT 16-MAY-2023
DEFINITION  Escherichia coli strain KFS-C10 plasmid pKFS-C10_8, complete
            sequence.
ACCESSION   CP125052
VERSION     CP125052.1
DBLINK      BioProject: PRJNA956323
            BioSample: SAMN34598743
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Gomi,R., Matsumura,Y., Yamamoto,M., Matsuda,T. and Harada,H.
  TITLE     Genomic surveillance of antimicrobial-resistant Escherichia coli in
            fecal sludge and sewage in Uganda
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Gomi,R., Matsumura,Y., Yamamoto,M., Matsuda,T. and Harada,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/09/2023 16:59:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,998
            CDSs (total)                      :: 4,876
            Genes (coding)                    :: 4,619
            CDSs (with protein)               :: 4,619
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 86 of 257
            Pseudo Genes (incomplete)         :: 177 of 257
            Pseudo Genes (internal stop)      :: 48 of 257
            Pseudo Genes (multiple problems)  :: 52 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KFS-C10"
                     /isolation_source="Fecal sludge"
                     /db_xref="taxon:562"
                     /plasmid="pKFS-C10_8"
                     /geo_loc_name="Uganda"
                     /lat_lon="0.19 N 32.35 E"
                     /collection_date="2018"
                     /collected_by="Hidenori Harada, Mai Tanaka"
     gene            complement(323..589)
                     /locus_tag="QL142_24985"
     CDS             complement(323..589)
                     /locus_tag="QL142_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHJ45416.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(593..1558)
                     /locus_tag="QL142_24990"
     CDS             complement(593..1558)
                     /locus_tag="QL142_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WHJ45417.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(934..1165)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 tctcggagat ggcatcatgc ttttaacgga ctttaaaaaa cggtgaacgg atgcggattt
       61 taatccgtgt ctgtgaggcg ttgagcaacg agcaaagcga ggcggtagaa agagagccag
      121 cggcaggctc aggacgataa tccggggaaa atgtaccact cgcagagtgt taacatttga
      181 catcggattg tctatcctgc attggtgtcg agttggcaag gctgaggata atgctgagga
      241 taatgcagga tagacagagc actagagatt gcatgcaatc tcgtacctat cacctcagcc
      301 tgccgctggc tcttttccag aactaagaat gcgttcttaa aaatccaaac ttgataccgt
      361 ttaaatctat tttcacaaaa agagttttta ccctctcgtt tgttttctga atatataaaa
      421 ttaattcatc ctcaaaacct tcgtgcggat gaaaaccaaa tgtgccaaaa cttaatccat
      481 caataagcaa aataaggttt ttaaacctgt caggaatcac cgcttcactt cttaaaaaaa
      541 ataaaccaat agtcttattt ccattcaggt attcaatatt aatattcata cgttaatccg
      601 atttatcctt ctcaggggcg cgtttgtaac gccgtttacc tgaatcccag acaaacgccg
      661 tccgcttccc gtcatcagtg ccatccccta catcgtcggc aatgacaaga tcctcattgg
      721 tttctcgatc caactggagg acgtttttta gcgccccacc ggtcgagata aaacggcgct
      781 tgtgaagctg ccgcgtcagc tcaagaaacc actcaggatc gtttgccata tcttccggtt
      841 taacggagta tttcagcgtt tcagcaaccg cgctttgcag ttgctcggca acgttggcca
      901 caacctcacc tgtcttagtt tttactgccc gaatatcgat attcggctca tagttcaccc
      961 gcaagcaatc gcgccagagt tctacccaac gttcgtgctt aacgtagttc ttccctttaa
     1021 accaagaagg ttgcaccatc agcagacagt gaaaatgcgg atgtgcgctg ccatccttac
     1081 ctcgcgtcac ctccgtagcc ctgatccacc cctgaacagg tgatagctcc tttcgctttt
     1141 ccattcgctt aaacgccgca ttcattgctg taaggactgt tccaagttca cctatctcgc
     1201 agttcctgac agttaacgtc agaaacaacc atcgggaaga cgggtaatcc acaacgattt
     1261 tcggtagagc ctgataaaaa cgggcttgcc acatgagggt tcttctccac tggcagacag
     1321 ggcaatgacg gacacggcaa aattgcgcac tacgaagccg taggcgcgtt tctccggttt
     1381 ccatgatggt agaccaacca aaacgtaaca aaccgctaca ggaggccata cggagtgcat
     1441 agcgctcgaa ttcagcagca gtctggtaga tacccccgac agaatcggta agcgtgcgct
     1501 gggtatccca aactgcatca cgggggctgt agtccgaaag gaatttatct tctgacatta
     1561 agcctgctcc gatctgggca ggctgtgatt tgatgtggta tattcatcac gtacctgtgc
     1621 attgcgagcc agtacatgtc accagagttt gccgctgagg tgacacattc gaaatgttgg
     1681 ggagtgttga cgcactcccc aacgccgatt aaaaacgcct caattctgct ccattttctt
     1741 tgacctgtca aaacccactc aaagcctcgt cattactggg ctaaaccact atttcatatc
     1801 cgccatttat cccttaagta taaatacaag gatcacgcgc gagaattgcg caaaaaataa
     1861 ccacaatttt aaccccccaa aacaagcacc cccgaacggc ttaccccaac cttttccaca
     1921 cacccgatca ccaaaacagg gaataaaaac aaaaccctgt cttacaggtg gatggcctca
     1981 cttcgtgaaa ccatccaccc cgcataagtt ttttaatcgg tggtgtcgtc aatcactaaa
     2041 tcggaacccc aaaggggacc cccacgattt agcatttgac gagattaac
//
