LOCUS       CP125051                2301 bp    DNA     circular BCT 16-MAY-2023
DEFINITION  Escherichia coli strain KFS-C10 plasmid pKFS-C10_7, complete
            sequence.
ACCESSION   CP125051
VERSION     CP125051.1
DBLINK      BioProject: PRJNA956323
            BioSample: SAMN34598743
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Gomi,R., Matsumura,Y., Yamamoto,M., Matsuda,T. and Harada,H.
  TITLE     Genomic surveillance of antimicrobial-resistant Escherichia coli in
            fecal sludge and sewage in Uganda
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Gomi,R., Matsumura,Y., Yamamoto,M., Matsuda,T. and Harada,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/09/2023 16:59:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,998
            CDSs (total)                      :: 4,876
            Genes (coding)                    :: 4,619
            CDSs (with protein)               :: 4,619
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 86 of 257
            Pseudo Genes (incomplete)         :: 177 of 257
            Pseudo Genes (internal stop)      :: 48 of 257
            Pseudo Genes (multiple problems)  :: 52 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KFS-C10"
                     /isolation_source="Fecal sludge"
                     /db_xref="taxon:562"
                     /plasmid="pKFS-C10_7"
                     /geo_loc_name="Uganda"
                     /lat_lon="0.19 N 32.35 E"
                     /collection_date="2018"
                     /collected_by="Hidenori Harada, Mai Tanaka"
     gene            complement(join(1906..2301,1..573))
                     /gene="mobV"
                     /locus_tag="QL142_24975"
     CDS             complement(join(1906..2301,1..573))
                     /gene="mobV"
                     /locus_tag="QL142_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WHJ45415.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRVEREKQRQMRSRGMGGMER"
                     /name="mobV"
     gene            1516..1842
                     /locus_tag="QL142_24980"
     CDS             1516..1842
                     /locus_tag="QL142_24980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001614210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHJ45414.1"
                     /translation="MNQAALITIANELNHDQMRVLMALLAELDYENYIQVAQIDIAEAL
                     TMQKTNVSRAVKNLIDFGIILEGPKIGRSKTYRLNPQFGWKGTVSNHKKALKNGLSVIQ
                     GGRT"
                     /name="hypothetical protein"
     Typing          complement(1910..2301)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.656"
                     /coverage="96.0"
                     /evalue="1.1699999999999999e-55"
ORIGIN
        1 gccacggttt atcgattcgt agtagtgctg aactgtctga tgcgtggcgc ggcttccctc
       61 gatgccgcgc tcaagaccca gtttttttac tgattcagcg taggtgctct gatcttcacg
      121 catctttgac cgcccgccaa taaactcttt cgcggacagc cgtccatcct gcgtcaatgg
      181 caccacgaac gcagacaggt gcggggtggc ctcgtcccgg tgaaccaccg ccgccacaac
      241 acgatccttc ccgtatttct tctccagcca ttgctcagag cgggcgaaaa actccgcctg
      301 ctgccgtggt gtggcctctt tccaccactc agggcttgct gtcataacgt attcaacggc
      361 cagtaccgca tctttacggc gtttttccgg cagcagctcc cgcacacgcc ccattgcctg
      421 atccgttgat ttggacacag agcagtagtt ttctggtgtc cgttcagcgt tagcgttcgg
      481 cgtctcacgc tctcggtagc agtgctgcaa cgcgctggcc acgcttccca ttcccgtcag
      541 ctttttgcag cgcattatcg cgtagttcgc catgttcccc cctgattccg gcattcattc
      601 agccaggcgg gggagcgcat tgcgatacct ccggcgggcc actatcaaga tgatagtgta
      661 ctcactagac tttgcttcgc aaaatcgttc gggaagctgc gcttccaaag gtcaaaatca
      721 acggcggtct ggcaggtgga aggcgtcgct ccgcgtcacc tcccaccact gccaagcctg
      781 ttttatccgg tgagtcgtca agggtaaatg gcactcgcta cgctcgccct tgacgaaatc
      841 aggcaatgaa tatcgttttt gaaagagcag gaaccgctcg ccgcaagaac ggattaggga
      901 gcggcatcgc cgcgagtctg tccgttcgag gaagcggaat ttccactccc cggacataga
      961 aatccgtacg ctccagactc aaaaaagctc gtttttcgat gtttaaaaat ggcgtttttc
     1021 agcctttatt ttccccctta atatatagat atagatacgg gaacccgcgc acaccaaggg
     1081 ctagagagaa gaatcgtact cacctcaagt actaatcgta ctcacctcaa gtactaatcg
     1141 tacttttcgc cgcagactca tgaccgaacg tagtgagcga atgagcgagg aagcggaatt
     1201 tccacttccc gtacatagaa attcgttcgc accgaacggg cagaaaaacg cctgattttg
     1261 ccgatgtatg agtacggaga gtaataagat acagaagggg atgtctcgga agccgcgcca
     1321 caagcggaaa gtttttgaca atagttatct ctagagataa cttagttatc ttgagagata
     1381 actatttttc ctgaaattgg agatttttag atgcggagaa tcacgcaggt agaccaaagt
     1441 acaggggagg aattaggtgg ttttgtagca gtgattcgtc ccaaacaaaa gtcatcattc
     1501 cagaggcact ttactatgaa tcaagccgcg ctaattacca ttgccaatga gctaaaccat
     1561 gaccagatgc gtgtactgat ggccttgctc gcagagctgg attacgagaa ctacatccag
     1621 gtcgcgcaga tagatattgc agaagcgctg actatgcaaa aaacaaatgt cagtcgtgcg
     1681 gtaaaaaacc tgattgattt cgggatcata ctggaaggac cgaagattgg gcggagcaaa
     1741 acgtaccgcc taaatcccca gttcggctgg aaaggtactg tcagcaacca caaaaaagca
     1801 ctgaaaaacg gcctcagcgt tatacagggc ggcagaacct gatctctgga atgccgcaaa
     1861 tggcgtcatt ttgcggcgtt agccctatcc tgcatcgcat caaaactaac gctccatacc
     1921 gcccatgcca cgcgatcgca tctgtcgctg tttctcacgt tcaacacgct gctgtttctc
     1981 cttctcctgg ttttgctgct taaaccgctg agccatcatc aggatttcgg tcatctgctc
     2041 tctggaaagc cccttaaaag gctctgtgac gctctgtagg cgtttctgct gcgcatccat
     2101 agtcttctgt agctcacgcg ctctttttgc gttctgggcg ctctgagagg ccatagcgat
     2161 ggttcctgca aagccatcgt tcactgcgtg gcttagacgt ttggctatgg ctgcctgatc
     2221 ttccacgtct ttggtgaaaa tgcccttccg caatacacgc ggctccagcg cctccggcga
     2281 gatcgatacc tggctacggg t
//
