LOCUS       CP124980                6647 bp    DNA     circular BCT 16-MAY-2023
DEFINITION  Escherichia coli strain KSE-D31 plasmid pKSE-D31_3, complete
            sequence.
ACCESSION   CP124980
VERSION     CP124980.1
DBLINK      BioProject: PRJNA956323
            BioSample: SAMN34598758
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Gomi,R., Matsumura,Y., Yamamoto,M., Matsuda,T. and Harada,H.
  TITLE     Genomic surveillance of antimicrobial-resistant Escherichia coli in
            fecal sludge and sewage in Uganda
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Gomi,R., Matsumura,Y., Yamamoto,M., Matsuda,T. and Harada,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAY-2023) Department of environmental engineering,
            Kyoto University, KyotoDaigakuKatsura, Nishikyo-ku, Kyoto-city
            615-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. v2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/09/2023 16:43:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,034
            CDSs (total)                      :: 4,910
            Genes (coding)                    :: 4,652
            CDSs (with protein)               :: 4,652
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 258
            CDSs (without protein)            :: 258
            Pseudo Genes (ambiguous residues) :: 0 of 258
            Pseudo Genes (frameshifted)       :: 82 of 258
            Pseudo Genes (incomplete)         :: 177 of 258
            Pseudo Genes (internal stop)      :: 54 of 258
            Pseudo Genes (multiple problems)  :: 49 of 258
            Pseudo Genes (short protein)      :: 4 of 258
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KSE-D31"
                     /isolation_source="Sewage"
                     /db_xref="taxon:562"
                     /plasmid="pKSE-D31_3"
                     /geo_loc_name="Uganda"
                     /lat_lon="0.19 N 32.35 E"
                     /collection_date="2018"
                     /collected_by="Hidenori Harada, Mai Tanaka"
     gene            312..830
                     /locus_tag="QL154_25030"
     CDS             312..830
                     /locus_tag="QL154_25030"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WHJ27386.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            837..1070
                     /locus_tag="QL154_25035"
     CDS             837..1070
                     /locus_tag="QL154_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WHJ27387.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1388..1807)
                     /locus_tag="QL154_25040"
     CDS             complement(1388..1807)
                     /locus_tag="QL154_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WHJ27388.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1888..2325)
                     /locus_tag="QL154_25045"
     CDS             complement(1888..2325)
                     /locus_tag="QL154_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WHJ27389.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            2585..4153
                     /gene="cea"
                     /locus_tag="QL154_25050"
     CDS             2585..4153
                     /gene="cea"
                     /locus_tag="QL154_25050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WHJ27390.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(4150..4377)
                     /locus_tag="QL154_25055"
     CDS             complement(4150..4377)
                     /locus_tag="QL154_25055"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WHJ27391.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            4539..4676
                     /locus_tag="QL154_25060"
     CDS             4539..4676
                     /locus_tag="QL154_25060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WHJ27383.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(5703..5894)
                     /locus_tag="QL154_25065"
     CDS             complement(5703..5894)
                     /locus_tag="QL154_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WHJ27384.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5958..6281
                     /locus_tag="QL154_25070"
     CDS             5958..6281
                     /locus_tag="QL154_25070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WHJ27385.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          5531..5618
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4962..5517)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3..1174
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.99"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6272..6645
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.2"
                     /coverage="93.0"
                     /evalue="7.73e-77"
ORIGIN
        1 cgcagacagg cctagaattg atgcctggca gacgctggtg aatcaccatt acgggctgca
       61 tgacccgaac gccccggaga accgcaggac gctgacactc cctgataacc tgcctgaaac
      121 gaaacaggcg cttgctgagg gcgtcacgcg aggtatagat gcactttacc atgccggaga
      181 gataaaaggc cgtcaggatg tgattcaggc gctcactgag gcggggctgg aagtggtcag
      241 ggtgacgcga agcagtatca gcattgcaga tccgaacggc gggaagaata tcaggctgaa
      301 aggagcattt tatgagcaat cttttgcaga cgggcgcgga gttcgagaaa aagctgaaag
      361 agagagccga atctacagag aaaatgctga acaacgagtt caggaggctc ggcgaatctg
      421 taagcgaggc tgtgacatca aacgagacga aaatcagaga cgctatagcc ctgttcacag
      481 cctcgacaga ggaatcgctg gaaaaacacc gggaaggggt gaaagaggcg atgatgcagc
      541 acaggaggga cgtgttaaag ctggcaggga atacgggcat gatgttactg gggatagtat
      601 ttctcctgtt taccgcgagt ggcgggacgc tctggtatct tggagggagg atacaggcga
      661 acctggaaga aatcaggaag caggaagaga cattgcagaa actgaacgcg aagacatggg
      721 gcgtggagtt tgtgcaggac gggaacagga aattccttgt ccttccgtac gggaaatcag
      781 cggaggtgat tccctttcag gggaaagagt gggtacatct gaaggagtga cacagagtga
      841 cagagctgga aacacatttg ctgaacgcct tagagcagct gcaacaggac tatatgcagc
      901 ggctgagcga atgggagagc gccttcgtgg aattgcagaa gatgttttcg cttacgcaac
      961 gggacaacgc gatgctgaac gagcgggtca tgcagttgag tcagcaggtg cagcacttga
     1021 gcgagcagac agaacgcttg agccagttat acagcgagaa ctggagataa gagaggaacg
     1081 gctgatacag gagcgcgaac atgtgttatc cctggaacgg gagcgtcagc cggaaataca
     1141 ggaacgcacg ctggatggcc cttcgctggg atggtgaaac catgaaaaat ggcagcttca
     1201 gtggattaag tgggggtaat gtggcctgta ccctctggtt gcataggtat tcatacggtt
     1261 aaaatttatc aggcgcgatc gcggcagttt ttcgggtggt ttgttgccat ttttacctgt
     1321 ctgctgccgt gatcgcgctg aacgcgtttt agcggtgcgt acaattaagg gattatggta
     1381 aatccactta ctgtctgccc tcgtagccat cgagataaac cgcagaaatc gtgtcagcca
     1441 gcagcctgga ttttttcggg gtaaggtctt tgctgcggta atcagtaccc gcaaattgag
     1501 ttgtgccccg gagtgagttt aaaaattcct ggctgtgaaa ctgctgaatg cgttgttgca
     1561 catcactccg tgattttcct gtcgctttat ctttagctcc catatcccag aactgccgaa
     1621 aaaaaggcac catcattcgt gttgtgtcgg ctttttgggt ggaaaagttg ggatcaaaac
     1681 cgtcatcaga agcatatacg taatgccggg tatgttgttc cacgctgccc tttggggtac
     1741 aggctgtgag agctactgtt gctgttatca tggcgataag acaaagtttg tttccggtac
     1801 gtctcatgag tgtctcccta ccttaaagta tttattctcg gtgagttatt atccgaagac
     1861 tattttatca tcagtctctg acagcgttca gcgaccgaac aacctggacc agaatccccg
     1921 tttcttttcc agttccagtg cctgcctgag ctgggctatc tcattttgta gctgttcccg
     1981 ttctgcttcc tggcgtctgc gatccatatc ctgtgcctgt ttatcctcaa gcatcagcgt
     2041 caggcattgt ttcagctcat tcagctcccg gagaatgcgt tctgtctgct gatcatgtgg
     2101 attttctgca tgtccctcac tgtgcctttc tggtgtctca ttctgcttta attcgccgta
     2161 tgcccggatc agttcactgg tttcaaactc tcgtcgtccg tccctgccgg tacggtaact
     2221 gacaaggcct gcgcgcatat cacggtaaaa ctgtgaacgc gatctgcctg tcatttttag
     2281 tgcgtcccgg agtgtgtgcc atgccataaa gtgacagtgt cccatagatg tctcatctca
     2341 tagtttcagt aaaacataat gaggtctgag aacggtaatg tttgtgctgg tttttgtggc
     2401 atcgggcgag aatagcgcgt ggtgtgaaag actgtttttt tgatcgtttt cacaaaaatg
     2461 gaagtccaca gtcttgacag ggaaaatgca gcggcgtagc ttttatgctg tatataaaac
     2521 cagtggttat atgtacagta tttattttta atttattgtt ttaaaagtca aagaggattt
     2581 tataatggaa accgcggtag cgtactataa agatggtgtt ccttatgatg ataagggaca
     2641 ggtaattatt actcttttga atggtactcc tgacgggagt ggctctggcg gcggaggtgg
     2701 aaaaggaggc agtaaaagtg aaagttctgc agctattcat gcaactgcta aatggtctac
     2761 tgctcaatta aagaaaacac aggcagagca ggctgcccgg gcaaaagctg cagcggaagc
     2821 acaggcgaaa gcaaaggcaa acagggatgc gctgactcag cgcctgaagg atatcgtgaa
     2881 tgaggctctt cgtcacaatg cctcacgtac gccttcagca acagagcttg ctcatgctaa
     2941 taatgcagct atgcaggcgg aagcagagcg tttgcgcctt gcgaaagcag aagaaaaagc
     3001 ccgtaaagaa gcggaagcag cagaaaaggc ttttcaggaa gcagaacaac gacgtaaaga
     3061 gattgaacgg gagaaggctg aaacagaacg ccagttgaaa ctggctgaag ctgaagagaa
     3121 acgactggct gcattgagtg aagaagctaa agctgttgag atcgcccaaa aaaaactttc
     3181 tgctgcacaa tctgaagtgg tgaaaatgga tggagagatt aagactctca attctcgttt
     3241 aagctccagt atccatgccc gtgatgcaga aatgaaaacg ctcgctggaa aacgaaatga
     3301 actggctcag gcatccgcta aatataaaga actggatgag ctggtcaaaa aactatcacc
     3361 aagagccaat gatccgcttc agaaccgtcc tttttttgaa gcaaccagac gacgggttgg
     3421 ggccggtaag attagagaag aaaaacaaaa acaggtaaca gcatcagaaa cacgtattaa
     3481 ccggataaat gctgatataa ctcagatcca gaaggctatt tctcaggtca gtaataatcg
     3541 taatgccggt atcgctcgtg ttcatgaagc tgaagaaaat ttgaaaaaag cacagaataa
     3601 tctccttaat tcacagatta aggatgctgt tgatgcaaca gttagctttt atcaaacgct
     3661 gactgaaaaa tatggtgaaa aatattcgaa aatggcacag gaacttgctg ataagtctaa
     3721 aggtaagaaa atcggcaatg tgaatgaagc tctcgctgct tttgaaaaat acaaggatgt
     3781 tttaaataag aaattcagca aagccgatcg tgatgctatt tttaatgcgt tggcatcggt
     3841 gaagtatgat gactgggcta aacatttaga tcagtttgcc aagtacttga agattacggg
     3901 gcatgtttct tttggatatg atgtggtatc tgatatccta aaaattaagg atacaggtga
     3961 ctggaagcca ctatttctta cattagagaa gaaagctgca gatgcagggg tgagttatgt
     4021 tgttgcttta ctttttagct tgcttgctgg aactacatta ggtatttggg gtattgctat
     4081 tgttacagga attctatgct cctatattga taagaataaa cttaatacta taaatgaggt
     4141 gttagggatt taattattct ttattgatat aaaaagtcct agcaatccaa atgggattgc
     4201 taggaccaaa caaagtagat tatatagcat aaataggttt aattttgcta cgggggcgtt
     4261 atttaggttt tttcttcttt cgaaaaaatc tttctttatg aagttaaaag ctatgtgttc
     4321 aatagcatat tttgaatatg gacatagaat agtgcttatc actattgcat atagcatctt
     4381 atctgacaca aggaaataat acccttcgct gttttttgtt ataaggtata tatatataag
     4441 tgtgcagtac aggccaaata aaatattttt tatgtagtat cttaagctca taaattaaac
     4501 ctcgccatat attcttttca ttttataagg atcgagttat gaggaaaaga ttttttgtgg
     4561 gaatattcgc gataaacctc cttgttggat gtcaggctaa ctatatacgt gatgttcagg
     4621 gagggaccat cgcaccatcc tcctcttcta aactgacggg gatcgcggtt cagtagaaaa
     4681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4741 aaaaccaccg ctaccaacgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4801 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4861 gtcgggccac tacttcaaga actctgtagc accgtttgtg ccatcatcgc tctgctaatc
     4921 cggttaccag tggctgctgc cagtggcgtt aaggcgtgcc ttaccgggtt ggactcaaga
     4981 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5041 agcttggagc gaacgaccta caccgaactg agataccaac agcgtgagct atgagaaagc
     5101 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5161 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5221 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5281 tggaaaaacg cctgctacgt ggccttcttc ctgttcctgg tcttttgctc acatgttctt
     5341 tccggcctta tcccctgatt ctgtggataa ctgtgttacc gtttttgtgt gagtcagtac
     5401 cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg
     5461 cctggacgtg cattttctcc ttacgcatct gtgcggcatt tcacacccgg catggcgtac
     5521 ttttcataca atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta
     5581 cgtgactggg tcagggctgc gccccgacac ccgctaaaac ctgctgacgc gccctgacgg
     5641 gcttgtcagc tcccggcatc cgctcacaga caagctgtga ccgtctccgg gagctgcatg
     5701 tatcagaggt tttcaccgtc atccccgaaa cgtgcgaggc agctgcggta aagctcatcg
     5761 gcgtggtcgt gaagcgattc acaaatatcg gcctgttcat ctgcgtccag ttcgttgagc
     5821 ttctccagca gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     5881 tcctgtttag tcactgatgc ctccgtgtaa gggggatttc tgttcatggg gtaatgatac
     5941 cgatgaaacg cgagaggatg ctcacaatac gggttactga tgatgaacat gcccggttac
     6001 ttgaacgctg tgagggtaaa caactggcgg tatggatgcg gcgggtctgc ctgggggagc
     6061 cggttgcccg ttccggaaaa ctgccgacac tggcaccgcc gttactgcgt cagctggccg
     6121 ccatcggaaa taacctgaat cagacagccc gtaaggtgaa cagcgggcag tggtcttccg
     6181 gtgaccgggt tcaggtggtg gccgcactga tggccatcgg ggatgagctg cgccggctgc
     6241 gtctggccgt cagggaacag ggggcgcggg atgatagtta aatttcatgc caggggaaaa
     6301 ggtggtggca gtggtccggt tgattacctg ctggggaggg agcgtaaccg cgaaggcgca
     6361 acggtgcttc agggtaatcc ggaagaagtc cgggaactca tcgatgccac gccatttgcg
     6421 aagaaataca cgtccggtgt tctgtcgttc gcggagaagg agctgccgcc gggaggacgt
     6481 gaaaaagtga tggcgagctt tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac
     6541 agcatcctgt gggtggagca ccaggacaag ggacggcttg agctgaattt tgtcattccg
     6601 aacatggagc tacagaccgg aaaacgcctc cagccgtact acgaccg
//
