LOCUS       CP124776                8654 bp    DNA     linear   BCT 11-MAY-2023
DEFINITION  Klebsiella michiganensis strain 2022CK-00495 plasmid unnamed10.
ACCESSION   CP124776
VERSION     CP124776.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN31152636
KEYWORDS    .
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00495
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.0x
            Sequencing Technology :: Oxford Nanopore GridION; Oxford Nanopore
            MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 13:40:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,393
            CDSs (total)                      :: 7,273
            Genes (coding)                    :: 7,098
            CDSs (with protein)               :: 7,098
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 59 of 175
            Pseudo Genes (incomplete)         :: 121 of 175
            Pseudo Genes (internal stop)      :: 30 of 175
            Pseudo Genes (multiple problems)  :: 29 of 175
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00495"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="unnamed10"
                     /geo_loc_name="USA"
                     /collection_date="2022-02-25"
     gene            742..1761
                     /locus_tag="QJQ59_36925"
     CDS             742..1761
                     /locus_tag="QJQ59_36925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000166 - nucleotide binding [Evidence IEA]; GO_process:
                     GO:0030541 - plasmid partitioning [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="WHA03622.1"
                     /translation="MTQDSKFIVGLDIGYSNVKIACGSAQSFEPSVSVYPAYATAEPET
                     DVALTKKSDDELKVYPYGNEWRVFTNRVGHRELHEKYHTSEMYMALFYGSLIKATNGRS
                     NIIDVLVTGLPVRIANSDEERQQLCKLFTGTHEVAPGKFVTVKEVVVLSQGVGIMNDVL
                     NREGLISDDDIEFSNILVIDPGYFSMDYVTFSRGDRKREFSGSSLNATSVIIDEIIRTL
                     EDEFPEEGTQEAERIETAFRRGDKTFNNGFRNVEIQPLVDKITPRIVGTVVAELLKRTR
                     SIGPVHIIISAGGGARFYDQSIRDAFKNARVIQSPNAVASNAIGYWHYGVEELNNNAQ"
                     /name="ParM/StbA family protein"
     gene            1785..2324
                     /locus_tag="QJQ59_36930"
     CDS             1785..2324
                     /locus_tag="QJQ59_36930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHA03623.1"
                     /translation="MKVHVTVDESIYPELHELMEATPVSKRARVLANLAFKACLLSNAA
                     LMTAELTTPSKGRNPSSKKKVSTTTGKKRRDGSKTDITALEERSFFNDGRKDEKTNEMS
                     SQPTLKSDANELAAGGESTHNNMNTSIPVSSKGAGVPERNSGNGDASEQRSIADGERSG
                     NNAPLVANTRKRRILG"
                     /name="hypothetical protein"
     gene            2278..2907
                     /locus_tag="QJQ59_36935"
     CDS             2278..2907
                     /locus_tag="QJQ59_36935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WHA03624.1"
                     /translation="MRRWWLTQENGAFWGKLSFNGVVMKKLLMTLCVVTGVIAVESPAK
                     AAIVDLRGTAWETYGKGECGLDPYLMYAIALWESKQRAGGGGWVAPSPWALNNSVYGAY
                     YPTSYEDAKKALVRYMKATPVTDIGISQVNYKWNGHYVSKPEDLLDIETNIKVQAKVLC
                     AAIKNNPDDIELAIGSYHTPNPKLRAKALNYARNVLYIWKRLIEND"
                     /name="lytic transglycosylase domain-containing protein"
     gene            2900..3424
                     /locus_tag="QJQ59_36940"
     CDS             2900..3424
                     /locus_tag="QJQ59_36940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHA03625.1"
                     /translation="MIKENISFLMCAGCIETRESSFYKSPFNFSEEDKLILNDKDQRAE
                     LFNVVEGLSLPIFIIEHDPYKATNQVDKFARMDSYILKLAVLIYSVRLKINEIGDSERF
                     LWTGSEEAFSFLSKMDHDGVIDCLIKREISIRPVCELCDILNGHWQMQQADGHHLMSRY
                     LQLRYKHWRVA"
                     /name="hypothetical protein"
     gene            3421..3918
                     /locus_tag="QJQ59_36945"
     CDS             3421..3918
                     /locus_tag="QJQ59_36945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0045893 - positive regulation of DNA-templated
                     transcription [Evidence IEA]; GO_process: GO:1902208 -
                     regulation of bacterial-type flagellum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FlhC family transcriptional regulator"
                     /protein_id="WHA03626.1"
                     /translation="MSPITKKEANSLITKMVNLGFRKVIICSSLGAPERQIDALKKSSG
                     QEDVGRGRLKIATTFLVMRQDKIEATNFMTIYLRIAKDPEKVVDIREIITAYEIYLKLH
                     HKFRPRNSSGIIIDANAAWILARDYRTEEIKMVTCNHCASHFISPYEDRPKHKCPFCDN
                     GT"
                     /name="FlhC family transcriptional regulator"
     gene            3947..4723
                     /locus_tag="QJQ59_36950"
     CDS             3947..4723
                     /locus_tag="QJQ59_36950"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHA03627.1"
                     /translation="MRMKALTVLTAAIILSGCAGGRGNDITPMPVDSSPSIALQYARAM
                     NLRLITDYDKGQYVIFNEDTGKSLRPLKEPVVVGTKGEVKSSGNGSADVIAGFVTHTAF
                     IPVLSVLTSRAPVPFSDGIARVATWQGGDAQANSRKLGNLLKEKLPLFDGFASTGYCRD
                     GIGYTTDVTRNVNANGTMSPIDTTTLPKPPFNAASNVQIMHVSDCFNALSQRRQNQARV
                     IEFSQSLGEQWAVFVPGSTEVPPVIYRNGTAYFFEK"
                     /name="hypothetical protein"
     gene            complement(4770..7460)
                     /gene="traN"
                     /locus_tag="QJQ59_36955"
     CDS             complement(4770..7460)
                     /gene="traN"
                     /locus_tag="QJQ59_36955"
                     /inference="COORDINATES: protein motif:HMM:NF011458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraN"
                     /protein_id="WHA03628.1"
                     /translation="MKKLFRERWFSIPICTLSIISHLGLISFVPYAIAADSTANATSTN
                     QKAQDAVNFYNTLPGISASGQGTVTYGSGQTVDLNSVYPDQQGSSTSLDSLKSVYGDDS
                     ATSKLGSTTGTTLTTENSLRGDAYRTMKNVSTKTSSLKDTDSIFNTHKDFLENQNEYMS
                     LLGDCSKQTILDPKDQVNHIPDYRECTRTVNVSGQYHLYHPYIAGILSHNSGPANIQSC
                     GDGCIELWVGTVGDNYWSGKCKIFEEEMSVNVKNTQAITSVKLVRAKWDDYMQVYVGGT
                     GRDDLVWAGPDGVDVFPPETSGRCERNTSWDKNPNVDITDKFKRSTDNGIVHFKTRTSV
                     SGEGEGYAKIEIRFDTSKVVTNDIWEHPDQGYDNFEKAVEQGYCQNYSVACNDAIIPDS
                     NGCATIDGVLVCQDDFVNTPPFGISPFCRNAVVTANCGQSEGVNNTCQQFENAGCNFIR
                     SACLDGASGDESGCWQASEVWDCGKDVVVPSTGSHEEYQCPGGVQCINGTCLAPTTEES
                     GDFATAAAMLQAATYAQNELACDTTGESNNDASTCQVFKGEAAVCKTAMGGWVDCCNQP
                     VNVSWIQYLQLTYYTLKVTDAVSVQAGMFEQGKGIFDMGSELASNAFDAITKPAMSAFD
                     SLTGNAGSAVAEKVSDMGISSAINEAVGELTKQVAQWTLDTFGSSVTNMLFEAGGSAAG
                     EAAVNSAGEMSASVQLSSTLVSAVSVVGYAYMAYQIANILVNIIWACTEDEFKLAVKKE
                     TKLAVFIDSWCQTKVLGQCIEKRSSYCTFNSQIGRIIQEQGRQQLGLNWGNDSKNPDCR
                     GLTLTEFGQIDFNKIDLSEWIGSLYEADLLPTADSVDLESITGQGNVLNIDGTRQNTLE
                     RFQSGVDGVDVQGTKETLEEQMRVK"
                     /name="traN"
     gene            7711..7917
                     /locus_tag="QJQ59_36960"
     CDS             7711..7917
                     /locus_tag="QJQ59_36960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHA03629.1"
                     /translation="MYGLGFYGLVPPLTAKPVFHYSNVDGPYTGKKQKTESLPMTPRVF
                     KEKLITAGLALASIPVIFMIGMS"
                     /name="hypothetical protein"
     gene            complement(7960..>8654)
                     /locus_tag="QJQ59_36965"
     CDS             complement(7960..>8654)
                     /locus_tag="QJQ59_36965"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHA03630.1"
                     /translation="TSAGVASTQSQAHSGISTVGSNSNHETIRPNVQADWDRNSDLGGP
                     VRSHNNEYNNVVEYQQSRSAAAMVATYQPNNIARDGMRIVNNLSSRLNNDNPTIQHERA
                     VAHQVATDNGNSYGAHALAEFYAAGKAGASGANLDAFRENAMATIMADTGGVRQSDGSV
                     IGGNKEYAARAVAAVESTFERQGANDLNTLNMATKGLWAAKNADELPSNETKHSWGEGI
                     TSPRSNGN"
                     /name="hypothetical protein"
ORIGIN
        1 tgcctataag gaaaacctgc ataagctcct caaaatttgg gacaaatttg acttttcact
       61 cttgcagttg ccatatacat acataggcag aacacacagt ttctgcctat gatagcgcca
      121 taacactatg acttatgcct atgtattagg cataacaaat gctatatgcc tattgctgtt
      181 ttctgaggat ataggtacaa ataacaaaca caagttttta aagggcgttt ttgtcgtgtt
      241 tttttaactc tgacacgtgt ttcttatggc aagtgagcac atgcagtaca cacattagct
      301 catgtattga gtatcacgtt tcaggagggc gtgatgacag cggtttaaca tttgcatcat
      361 ttgttcgaat gtcgcgcatt aaaaaaaagg aaaagaaatt cctgatttgc tattcgaccc
      421 ctgaaccaag cgtgagatta tagttgtgcc atcgaaagca acgcgctcat tcgacgtttg
      481 aatgacattc aaatgacgtt tagagagatg ccattcagct ttcaaaatag ttttaaatga
      541 cattcagatg cttgtcattt attgttgttt ttaatctcga agaaggtatg ctttgtaagc
      601 tcttgtaaat cgacacactc tgatgtcata agtcttacga acataaagag cctgtagcag
      661 gcacttttaa aacctgctag atgcaaggga ttactgagtt agggtttagg cgttagccgt
      721 ttttaaatca aaggtgaaag aatgacacaa gactcgaagt tcatagtcgg tttggatatc
      781 ggttacagca acgtgaaaat agcttgtggt agcgcccaat catttgaacc atcggtatcg
      841 gtttatcctg cttatgctac tgcggaaccg gaaacagacg ttgcacttac caaaaaaagt
      901 gatgatgaat tgaaagttta cccttacggg aatgagtggc gtgtattcac aaatcgtgtc
      961 ggtcatcgcg aacttcatga gaagtaccac accagtgaaa tgtacatggc cctcttttat
     1021 ggctcattga ttaaagccac gaatggccgt agcaacatca ttgatgtact ggttactgga
     1081 cttcccgtgc gaatcgcaaa ctcagatgaa gaacgtcaac agctttgtaa attattcacc
     1141 ggcacacatg aagttgcacc cggcaagttt gttacagtaa aagaagttgt tgttctttcg
     1201 cagggtgtgg gaatcatgaa cgatgtcctt aatcgagaag gcttgatctc cgatgatgac
     1261 attgagttct caaatattct tgttatcgat ccggggtatt tttcaatgga ttacgtaaca
     1321 ttctctcggg gagacaggaa gcgagagttc agtggttcct ctctaaatgc aaccagtgtc
     1381 atcattgatg aaatcattcg cacattggaa gatgagttcc cggaagaagg gactcaggaa
     1441 gctgaacgta tcgagacagc atttcgacgt ggtgataaaa ctttcaataa cggttttcgc
     1501 aatgtagaga ttcagcctct cgttgataaa atcaccccac ggattgtcgg aacagtggta
     1561 gctgaactcc taaaacgtac acgctccatc ggccccgttc acattatcat ttctgctggt
     1621 ggtggtgctc gcttctatga ccagtcaatt agagatgcat tcaaaaacgc tcgtgtaatt
     1681 cagagtccaa acgctgtagc gagcaacgca atcggctact ggcactatgg ggtagaagaa
     1741 ctcaataata acgcacagta atagccacgg ggtattgaat tgatatgaaa gtgcatgtga
     1801 ccgtggatga aagcatatac cctgagcttc acgaattaat ggaagctacg ccagtgagca
     1861 agagagcgcg agtgcttgca aatcttgctt tcaaagcgtg cttactaagc aacgccgcac
     1921 ttatgaccgc tgaattgact acgccctcaa aggggcgtaa tcctagcagc aaaaagaaag
     1981 tttccacgac cactggaaaa aaacgtcgtg atggtagtaa aacagatata accgcacttg
     2041 aagaacgttc gtttttcaat gacggtagaa aagatgagaa aacgaacgag atgagcagtc
     2101 aaccgacgct caaatcagat gcaaatgaat tagcagcagg aggtgaaagc acacacaata
     2161 atatgaatac atcgatacct gtaagttcaa aaggcgcggg ggtgccagaa agaaactcag
     2221 gtaacggaga tgcttctgaa cagcggagta ttgctgacgg ggaaagaagc ggaaacaatg
     2281 cgccgctggt ggctaacaca agaaaacggc gcattttggg gtaagttaag ttttaatggg
     2341 gtagttatga aaaagttgct tatgactctg tgtgtggtca cgggggttat agcagtggag
     2401 tcacctgcga aagcggctat cgtcgatctt cgcggaactg cttgggaaac ttatggtaag
     2461 ggtgagtgtg gtcttgaccc ttatttaatg tatgcaattg ctttgtggga aagtaaacaa
     2521 agagctggtg gtggcggttg ggttgcacct agtccttggg cacttaataa ctctgtttat
     2581 ggggcttact atcccaccag ttatgaagat gccaaaaaag ctcttgtgcg ctacatgaaa
     2641 gcaacgccgg ttactgatat aggcatttca caggttaact ataagtggaa tggacactat
     2701 gtttcaaaac cagaagacct tctggatatc gaaaccaaca tcaaggtgca ggcaaaagtt
     2761 ctttgcgctg ctatcaaaaa caaccctgac gacatcgaac tagcaatagg aagctatcac
     2821 acgccgaatc caaagctacg tgctaaagct ctaaattatg cccgtaatgt tttatatatt
     2881 tggaagaggt taattgaaaa tgattaaaga aaatatttca tttttgatgt gtgctggatg
     2941 cattgaaact cgtgaaagca gtttctacaa aagccctttc aatttttcag aagaggataa
     3001 acttattctc aatgataaag accaaagagc cgagcttttc aatgtggttg aggggttgtc
     3061 tttaccaatc ttcatcattg aacatgaccc atacaaggct acgaatcagg tagataagtt
     3121 tgcaagaatg gactcctata ttttaaagct tgcggttctg atttatagcg taaggcttaa
     3181 aataaatgag attggggatt cagagcgttt cctctggaca ggctccgaag aagcattttc
     3241 atttctctcc aaaatggatc atgacggagt tatcgattgc ctgataaaaa gggagatctc
     3301 aattcgaccg gtttgcgaac tctgcgacat actcaatggt cactggcaaa tgcaacaggc
     3361 cgacggccat catttgatga gccggtatct gcaactccgt tataagcatt ggagagtggc
     3421 atgagtccaa taacaaagaa agaagcaaac tccctgatca caaagatggt gaacttgggt
     3481 ttcagaaaag tgattatttg ctcgtcactt ggtgcgccag aacgtcagat tgacgcgctc
     3541 aaaaaaagca gtgggcaaga ggatgttggt cgcggcaggt tgaagatagc aaccactttt
     3601 cttgtgatgc gacaagataa aattgaagca actaacttca tgactatcta cctgagaatc
     3661 gcaaaagacc ccgagaaggt agtggacatc cgagagatca tcacagcgta tgagatctat
     3721 ttaaaactac accataaatt tcggcctcgg aattcatctg gtatcatcat agatgcaaac
     3781 gcagcttgga tactggctcg tgattacaga acagaagaga taaagatggt gacatgtaac
     3841 cactgtgcaa gccacttcat ctcgccatat gaagacagac cgaaacacaa atgtccgttt
     3901 tgtgacaacg gaacctgata aggtaacgat cttgagggaa tctacgatga gaatgaaagc
     3961 actaacggtt cttacggcag caataatttt gagtggctgt gctggtgggc ggggaaatga
     4021 cattacccct atgcctgtag acagttctcc ctccatcgcc ttgcagtatg caagggcgat
     4081 gaacctgcgt ttaattactg attacgataa aggccagtat gtcattttca atgaagatac
     4141 cgggaaatca ctacggcctc ttaaagaacc ggtcgtagtt ggaacaaaag gtgaggtcaa
     4201 gtcgtctgga aatggttctg ctgacgttat cgctggcttt gttacgcata cggcatttat
     4261 ccctgttcta agcgtattaa catcacgagc gccagttcca ttctccgatg ggatagctcg
     4321 tgttgctact tggcagggag gcgatgctca ggcaaacagt cgaaaactgg ggaatctcct
     4381 gaaagaaaaa cttcctctgt ttgatgggtt tgctagcacc ggatactgta gagatggtat
     4441 cggctataca acagatgtaa cacgtaatgt caatgcaaac ggcactatga gtcccattga
     4501 cacaacaacg cttccgaaac cacctttcaa tgctgcgtcg aatgttcaaa ttatgcatgt
     4561 cagtgattgc tttaacgcgt tatcacaacg cagacaaaat caggctaggg tcatcgagtt
     4621 tagtcagtca ttaggcgaac agtgggcagt tttcgttccg ggaagcacag aggttccacc
     4681 tgtgatttat cgaaatggga cggcttattt ttttgaaaaa tagtttatgt caaccaacaa
     4741 aaaacccgcc gtagcgggtt tttttattgt tatttcaccc tcatttgctc ttcgagagtt
     4801 tccttggttc cctgcacatc tacaccatca acaccactct ggaagcgttc taatgtattt
     4861 tgtcgagtac cgtcaatatt caaaacatta ccttgtcctg ttatgctttc cagatccacg
     4921 ctatcagcgg taggtaacag atcagcttcg tacagcgaac ctatccattc cgaaaggtca
     4981 attttgttaa aatcgatctg tccaaattca gtaagggtta gaccacgaca atccgggttt
     5041 tttgagtcat tcccccagtt cagtcctaac tgttgacgtc cttgttcctg tattattcga
     5101 ccaatttgcg agttaaacgt acagtaactt gacctcttct ctatgcactg gcccagaact
     5161 ttcgtctgac accagctatc aatgaacaca gccagctttg tttctttttt taccgccagc
     5221 ttaaattcat cttctgtgca ggcccagatg atattaacga gaatgttggc aatttgataa
     5281 gccatatagg cataacctac aacagataca gccgatacaa gtgtcgatga tagctggact
     5341 gatgcagaca tttcccctgc tgagttaact gctgcttctc cagcggcact tccaccagct
     5401 tcaaaaagca tatttgtcac actggaaccg aacgtatcca aagtccattg tgcaacttgt
     5461 ttggtcaatt caccgacggc ttcattgatg gctgatgata tacccatatc cgaaaccttt
     5521 tctgccacag cagaacccgc gttaccggtc aaagagtcaa atgcagacat tgcaggcttt
     5581 gtgatggcat cgaaggcatt gctggcaagt tcagaaccca tatcaaagat ccctttaccc
     5641 tgctcaaaca tcccagcctg aacagatacc gcgtccgtca cttttagtgt gtaatacgtg
     5701 agctggaggt attgaatcca gcttacattg acaggctgat tgcaacagtc aacccatcca
     5761 cccatagcag ttttacacac cgcagcttcg cctttgaata cctgacaggt tgaggcatcg
     5821 ttattcgatt cacccgttgt atcacaggcc aactcattct gagcataggt agctgcttgc
     5881 agcatagcgg ctgctgtagc gaagtcaccg ctctcctcag tagtaggagc aaggcatgta
     5941 ccattgatgc actgtacacc tccagggcat tggtattcct catggctgcc agtagagggt
     6001 acgaccacat ccttaccgca gtcccatact tcacttgcct gccaacaacc actttcatcc
     6061 ccactagcgc catccaaaca ggcgcttcga atgaaattac aacccgcatt ctcaaattgt
     6121 tgacaggtat tattgactcc ctcactttgt ccacagttag ctgttactac tgcatttcgg
     6181 cagaatggcg atattccaaa tggaggggtg tttacaaagt catcttggca aactaaaaca
     6241 ccgtcaatag ttgcacatcc attagaatca ggaattattg cgtcgttaca cgcgactgag
     6301 tagttctggc agtaaccttg ttctactgct ttttcaaaat tgtcataacc ttgatcggga
     6361 tgttcccaaa tatcatttgt aacaaccttg ctcgtatcga agcgtatttc aattttcgca
     6421 taaccttcac cttctccact tacagaggtt cttgttttaa aatgtacaat accgttatca
     6481 gtagaacgtt taaacttatc agtaatgtcc acatttggat ttttgtccca actggtattc
     6541 cgttcacagc gaccagaggt ttctggggga aatacatcta cgccatccgg gccagcccaa
     6601 accagatcat cgcgaccagt tcccccgaca taaacctgca tgtagtcatc ccacttagct
     6661 ctaacaagct ttacactagt aattgcttga gtgtttttaa cgttaactga catttcctct
     6721 tcaaagattt tacatttacc tgaccagtag ttatcaccaa ctgttccgac ccaaagttct
     6781 atacacccgt caccacagga ttgaatgttc gcagggccgc tgttatgaga aagtatccct
     6841 gcaatgtagg gatgatagag atggtactga cctgaaacat tgacagttct ggtgcattct
     6901 ctgtagtcag gaatatgatt aacctgatct ttcggatcga gaattgtttg tttcgaacag
     6961 tcaccgagca agctcatgta ctcattctgg ttttcgagga agtctttgtg tgtattgaaa
     7021 atagaatctg tatctttaag tgaggatgtt ttggtggaga cgttcttcat tgtgcgatat
     7081 gcatcaccac gcagggaatt ctcagtagtt agtgttgtac ctgtggtgga acccagtttg
     7141 cttgtggctg agtcatcacc atagactgat tttaatgagt caagggaagt tgatgatcct
     7201 tgctggtcag ggtacacaga gttaaggtca acagtctggc cgcttccata cgtcaccgtc
     7261 ccttgcccac tggctgaaat gccgggaagc gtgttataga aattcacagc atcttgtgct
     7321 ttctgattag ttgaagttgc atttgcagtg ctatcggctg cgattgcata aggtacaaaa
     7381 ctgattagcc cgaggtgaga tattatagac agcgtgcaga tgggtatcga aaaccaccgc
     7441 tcacggaaaa gttttttcat ggttagttca cctttttatc taaaactaac gtctaatcgc
     7501 ttaaaaaaat ccacttaggc tattcgggtc aggaatttga cgtgagacta tatggatatg
     7561 aacagtgttc acgataatgg ttggcgctgt tgataagcaa caaacaaagg ggacaaaagc
     7621 ggggggtttt aacagatagt tggtgagtcc tgtagaatct tctatactgt ttttttaatg
     7681 cgtatttctc agtagttgta aggaggggtt atgtatggtc ttggttttta cgggttggtt
     7741 cccccattaa ccgcgaaacc tgtatttcac tattcaaatg tggatgggcc gtatacgggg
     7801 aaaaaacaaa aaacagaatc tttgccaatg acaccgcggg ttttcaagga aaaattgatc
     7861 actgcgggac ttgctttggc gtctatcccc gtcatattta tgattggtat gtcataaaaa
     7921 aacccgcttc aagcgggttt ttttatagta gtaaaatagt taatttccat tacttctggg
     7981 gctggtgatc ccttcacccc aagaatgctt ggtctcatta cttggtagct catctgcatt
     8041 ttttgcggcc cacagacctt tggtagccat attcagagtg ttaaggtcat tagcgccctg
     8101 acgttcaaaa gtggactcca cggctgcaac tgctcgtgca gcgtattctt tattcccacc
     8161 aataactgaa ccgtcggatt gcctgacccc gccagtatcg gccatgattg ttgccatcgc
     8221 attttcacgg aaagcatcga gattagcacc tgatgcacca gctttacctg ctgcgtaaaa
     8281 ttctgcaaga gcatgagcac cataactgtt accattatca gtcgcaacct gatgagctac
     8341 tgctcgttcg tgttgaattg tagggttgtc attgttcaaa cgagatgaca ggttatttac
     8401 aatccgcata ccgtcacgag cgatattgtt tggctgataa gttgcgacca tcgctgccgc
     8461 agaacgactc tgctgatact caacgacatt attgtattcg ttgttatgag aacgaaccgg
     8521 cccgcccaga tcagaatttc gatcccagtc agcttgtaca tttggccgta ttgtttcgtg
     8581 attgctgttt gaacctacgg tagatatacc tgaatgagct tgagattggg ttgaggctac
     8641 gcctgctgat gttg
//
