LOCUS       CP124772                5172 bp    DNA     linear   BCT 11-MAY-2023
DEFINITION  Klebsiella michiganensis strain 2022CK-00495 plasmid unnamed12.
ACCESSION   CP124772
VERSION     CP124772.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN31152636
KEYWORDS    .
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00495
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.0x
            Sequencing Technology :: Oxford Nanopore GridION; Oxford Nanopore
            MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 13:40:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,393
            CDSs (total)                      :: 7,273
            Genes (coding)                    :: 7,098
            CDSs (with protein)               :: 7,098
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 59 of 175
            Pseudo Genes (incomplete)         :: 121 of 175
            Pseudo Genes (internal stop)      :: 30 of 175
            Pseudo Genes (multiple problems)  :: 29 of 175
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00495"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="unnamed12"
                     /geo_loc_name="USA"
                     /collection_date="2022-02-25"
     gene            <1..2906
                     /locus_tag="QJQ59_05910"
     CDS             <1..2906
                     /locus_tag="QJQ59_05910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGZ97654.1"
                     /translation="DDSALAVEVINNAGTLQPTGRKMPSQAAVAANTSVITGLDLDRVD
                     VSPASLGTGQYYQLSTGTIATTTDTNWSASSPVSVAGYKRLILSGSFLYRAAVAPLLFV
                     DSAGTVISVQSLGIPMSSDNVWSSRSEVQVDIPAAAVSALISTYAEPAVAVYAAAQAFR
                     ESGVALSASKTKKRVLNWIPNTYMNIATGVLTSSSDTFAAARVPVAPGDVFTLSCSLKG
                     APNQIRLIGYFDSNETLIGTFYPGTGSGTVSTITNLTFRVPKNAASMVITAYSPNSSTV
                     LMQASVFDRAALDIQTAADTAGNLAKSSVLPFYSGRYWKNTNGALTVVNDAQFCAFYPV
                     QVTAGDVYRIKSNLTTGNPSLVSMVVFKGANGDLVGTAQPAPGSGAFAAADITVTVPAG
                     AVLMLLTAYSPYIDIVYQGSAVEKIGSSVAGIAASFTQQLSINFSKGYYWNPTSGTLTS
                     TTDPNWRAFDAIPVSPGESYRVIATEFSGSIYVVVFKNASGAVVSKSQLGPGGSSIVAV
                     DVPVTVPAGATSMCVTAYTDNVTITKMVNYRADLRKDIIQRDFDTGLRLLSPKGLDGYY
                     WHQNTGVRTLITSDWAYFSVDPITVSPGEVYRIICAEFSGNPANVYLALFKDSSGAIVG
                     RSYAGPGSGVLQAVDTNVTVPAGATSMCVTGHSRNLQIIKSGGMISKLPGNSQTQASSP
                     LDYWKGKKIVWLGTSIPAGSGTNSYPYMLAQRLGANIVNQSVGSSPIRGGLDAFVTTDD
                     PYGWTGSSYTRVTRALSHSVDIKQSFIDNYDSKWKALVTGGPASLSDTDKANILSYSYA
                     NRLSGNLDSDLFVIDHGINDYLWIQERGGDVASLLTPAVDTRNINTFYGGINTVIDYIL
                     SQNPRARILVIGFYENELRPQVSQIQLKSAQLWEYQIVKLWEKTGWSQQVLTGTDGAGK
                     TITQYWMPDNLHPHSDTTGKANTLLANILEMEIRSVR"
                     /name="hypothetical protein"
     gene            complement(2965..3711)
                     /locus_tag="QJQ59_05915"
     CDS             complement(2965..3711)
                     /locus_tag="QJQ59_05915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGZ97655.1"
                     /translation="MPIPAVFIDSDLPETPGMKDIDDFIVKNWFTDFPAVDGTKLGGYY
                     FGNPVSNLAMNSFNNAAPATLNGSFDNSQGYINVDVNNYLDTTLKAPVALKVLAVIRRP
                     SNPSVANYFISDFSGTGVAGLGFGLGIATDGKLRIVGQNDATTAVASVDFPSSIAVGDL
                     CAIAAHVIQGAVYVAIYNPATATYVAGNTAMSGTRSAGTKNILIGRKTDNNTSTASTAI
                     KSAVLINGEITGAQQLAAMQYLLAMD"
                     /name="hypothetical protein"
     gene            complement(3714..>5172)
                     /locus_tag="QJQ59_05920"
     CDS             complement(3714..>5172)
                     /locus_tag="QJQ59_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="WGZ97656.1"
                     /translation="DDSALAIEVINNGGTLEATGRQMPSVVTIGKKLDERIPLGDFKPS
                     YFPLWYDRNGKVIVWCKDGYPDFSGFGENAQSIVAAIPNEWAQYHLRQFDFSPSYFPLW
                     FDRKGKVLAWSKNGTVDFVIGESIQSQVKELVGANSSSSFIEGDQFKFIFKRGRIAAGV
                     AGSLNVGFGMDSWGEKSTIPQALINILGGGYKDPGYISCSTRTDGVMAGITLVVTNFTK
                     YDGDNENNNAPPPYGSGPDGNAYWNNNTVGTLTWSNVTATDLSLFYFDSTGNFTVAIDG
                     GAPVTITGTNSGTVKKYDITGLTATAHTVVVTSSGTGVVSILCMYGKNSARTNGITVSR
                     MGNGGAMAKDYLHWQDYITPIVTHFDLDLFFMILGTNDFRKSAGIAEFVNGLQVVIDKY
                     KAATPGICICLVSAAQSNATGTPTLSEYDEAMRTLAIENNVNFISLYQLFPKTYDNSGG
                     AWADALHLSDRGAYVLARKIKNEFFQE"
                     /name="GDSL-type esterase/lipase family protein"
ORIGIN
        1 cggatgacag cgcgttagct gtagaagtga tcaataacgc cgggacgctg cagcctaccg
       61 ggcggaaaat gccatctcag gcggctgttg ccgcgaacac atccgtaatt acaggcttgg
      121 atcttgatcg tgttgatgta tctccagcgt cgctggggac tggccagtat tatcagctct
      181 ccacagggac aatcgcgacc accactgaca ccaactggtc ggcgagctcc cccgtttcag
      241 tggctgggta taaacgctta attttgtctg gttcgttttt gtatcgtgct gccgtcgcac
      301 cgcttttgtt tgttgactcg gcagggactg ttatttcagt tcagagcctg ggcattccca
      361 tgtcctcgga taatgtctgg tcatctcgtt cagaggttca ggtcgatatt cccgccgcag
      421 cagtcagcgc actgatatca acctatgccg aaccggctgt ggctgtctat gcagccgcgc
      481 aggctttccg ggagtcaggc gttgccctat cggccagtaa aacgaaaaaa cgggttctga
      541 actggatacc taacacatac atgaatattg cgacgggcgt tctgacatcc agctcagaca
      601 ccttcgcggc cgcccgtgtg ccggtggcac caggggatgt attcacactc agttgctcgc
      661 tgaagggggc accaaaccag attcgcctga tcggttactt tgacagtaac gagactctca
      721 tcggtacgtt ctacccaggg acagggagcg gcacggtatc cacgatcacc aacctgactt
      781 tccgcgtgcc aaaaaacgcg gcgtctatgg tgatcaccgc gtattccccg aactcgtcaa
      841 cggtgctgat gcaggcttcc gtcttcgacc gtgcggcgct tgatatccag accgctgctg
      901 acacagccgg aaacctcgcg aagtcgtcgg tgctgccttt ctatagcggg cgctactgga
      961 aaaacaccaa tggtgcattg accgttgtga acgacgcgca gttttgcgcc ttctacccag
     1021 tacaggtgac cgccggtgat gtgtaccgca tcaagtctaa cctgacgacg gggaacccgt
     1081 ctctggtatc gatggtggtt ttcaagggcg ccaacggtga tctggtaggc actgctcagc
     1141 ccgcccccgg gtccggggcc tttgcagcag cggatatcac cgtaaccgtc ccggctggtg
     1201 cggtgctgat gcttttgact gcatacagcc catatataga catcgtctat cagggtagcg
     1261 ctgtagaaaa aatcggatcg tcggtggcgg gcatcgctgc aagttttact cagcaactca
     1321 gcattaactt ttcgaaaggg tattactgga acccaacatc cggcacctta acgagtacca
     1381 ccgatcctaa ctggcgggca tttgacgcca tacccgtttc gcctggtgag tcgtatcgtg
     1441 tcattgcgac tgagtttagc ggtagcattt acgtcgtagt tttcaaaaat gcgagtggtg
     1501 cggtcgtaag caagtctcag ctcggtcctg gtgggtcttc gatcgtcgct gtcgatgtcc
     1561 ccgttaccgt gccggctggc gctacgtcca tgtgcgttac ggcctatacg gataacgtga
     1621 ccatcacaaa aatggtcaac tacagggcag atttacggaa ggatattatt cagcgcgact
     1681 ttgataccgg gttgcgtctg ctttctccta aagggctgga cggctattac tggcaccaga
     1741 acacgggggt caggacgctg attaccagcg actgggcata tttctctgta gacccgatca
     1801 cggtttctcc gggggaagtg taccggatca tctgcgccga gttttctgga aacccggcaa
     1861 acgtatatct cgcgctgttc aaagactcct ccggggccat tgtggggcgc agctatgcag
     1921 gacctggctc cggcgtcctt caggctgttg ataccaacgt taccgtgccg gctggcgcta
     1981 cgtccatgtg cgttaccggc cacagcagga atttgcagat cattaaatct ggagggatga
     2041 ttagcaaact tccggggaac tctcaaaccc aggcgagcag cccgcttgac tactggaagg
     2101 ggaaaaaaat agtctggctg ggaacctcaa ttcctgcagg aagcggaaca aactcctacc
     2161 cgtatatgct ggcccagcgc ctgggggcga acatagttaa ccagtccgtc ggctcatctc
     2221 cgattcgtgg tggtctggat gccttcgtca ccaccgacga tccatatgga tggacgggaa
     2281 gctcatatac ccgcgtcacg cgtgccttgt cgcattccgt cgatattaag cagtcattta
     2341 tcgacaacta cgattcgaaa tggaaggcgt tagtgacagg tggcccggca tccctgtcag
     2401 atactgataa ggcaaatatc ctgagttatt cctatgccaa ccggctgtcg ggtaacttgg
     2461 attcggacct gtttgttatc gatcacggga ttaacgacta tctgtggatt caggagcgag
     2521 ggggagatgt tgccagtctg ctgacgcctg cggtcgatac ccgtaacatc aatactttct
     2581 acggcggcat aaatacggta attgactaca tcctgagcca gaacccacgg gcccgcattc
     2641 ttgttatcgg cttttatgaa aatgagttga ggccgcaggt ttctcagata cagttaaaat
     2701 ccgcgcagct gtgggagtat cagatcgtga agttgtggga gaagacaggc tggagccagc
     2761 aggtattaac cgggactgat ggcgcaggga agaccataac ccagtactgg atgccggaca
     2821 acctgcaccc tcactcggat acgacaggaa aggcaaacac attgctggcg aatattcttg
     2881 aaatggaaat caggtcggtt cgttaacttt caacaccaag cggctatcat gaaaactgac
     2941 agccgcaacg catttctttc tacattaatc cattgccagc aggtattgca ttgccgcaag
     3001 ttgctgagct cctgtgatct cgccatttat caggactgca gacttgattg ccgttgaagc
     3061 ggttgacgta ttgttatctg ttttcctgcc aataaggata ttctttgtac ctgctgaacg
     3121 agtacctgac atagcggtat ttcctgcgac gtacgtcgca gttgcaggat tgtagattgc
     3181 aacatatacc gctccctgaa taacgtgtgc cgctattgcg cacaggtcac cgaccgcaat
     3241 tgaagaaggg aaatccaccg atgccaccgc tgtggtagcg tcgttttgac caactatccg
     3301 caatttaccg tcagtggcaa tgccaagccc aaatccgagc cccgctactc ccgtaccgga
     3361 aaagtcagaa atgaaataat ttgccacaga aggatttgat ggtcgtctga tgaccgccag
     3421 cacttttaag gcaacaggcg ctttaagtgt ggtgtcgaga tagttattaa catcaacgtt
     3481 aatataaccc tgtgagttat cgaatgatcc attcaatgtc gcaggtgctg cattgttaaa
     3541 tgagttcatc gccaggttac ttacgggatt tccgaagtaa tacccaccca gttttgttcc
     3601 gtcgacagca gggaagtctg taaaccagtt ttttacgata aaatcgtcaa tatctttcat
     3661 tcccggcgtt tccggcaagt ctgaatcaat gaaaacagct ggtatcggca taattactcc
     3721 tggaaaaatt cgtttttgat tttacgcgcc agtacatatg cgccacggtc tgacaggtgc
     3781 aatgcgtcag cccatgcgcc accggagtta tcgtacgtct ttggaaacaa ctggtagagg
     3841 ctgataaaat taacgttatt ctcgattgcc agcgtacgca tggcttcatc atattcagat
     3901 aaggtaggcg ttccggttgc gttgctttgc gccgcagaaa ccaggcaaat acaaattccc
     3961 ggcgtggccg ctttgtattt atcgataact acctgtaacc cattgacaaa ctcagcgata
     4021 cccgcgcttt tcctgaaatc gttggtacca agaatcataa aaaataaatc gaggtcaaag
     4081 tgagtaacaa tcggggtaat atagtcctgc cagtgcaaat agtcttttgc catagcccca
     4141 ccattcccca tgcgcgaaac ggtgattccg ttggttctgg cgctattttt tccatacata
     4201 cagagaatgg aaacaacacc cgtccctgaa ctggttacaa caacagtatg tgcagtcgca
     4261 gtcagtccgg taatgtcgta tttttttacc gtgccgctat ttgttccggt gatagtcaca
     4321 ggtgcaccac cgtcgatagc gacagtaaaa ttacccgtgc tgtcaaaata aaataatgac
     4381 aggtcagtgg ccgtaacatt cgaccaggtc agagtcccta ccgtattgtt attccagtag
     4441 gcgttcccgt cagggcctga gccgtacgga ggcggtgcat tgttgttttc attatcaccg
     4501 tcatatttcg taaagttggt gactaccaaa gtgattccgg ccataacccc atctgtcctg
     4561 gtagagcaac tgatataccc ggggtctttg tatcctccac ccaggatatt aataagtgcc
     4621 tgcggtatgg tgctcttttc tccccatgaa tccataccaa atcccacatt gaggctgcct
     4681 gctacgcccg cagcgatgcg accccgctta aaaataaatt taaactgatc gccttcaatg
     4741 aatgaactcg aagaatttgc gccaaccagt tctttaacct gactctgaat gctctctcca
     4801 atgacaaaat ccaccgtgcc gttcttactc cacgccagga ctttgccttt tctgtcgaac
     4861 cagagaggga agtacgaggg actaaagtcg aactgccgaa gatgatactg ggcccactcg
     4921 ttagggatgg ctgctacgat gctttgtgcg ttttccccga aacccgagaa atctggatat
     4981 ccatctttgc accagacaat gacttttcca tttctgtcat accatagtgg gaaatatgat
     5041 ggtttgaaat cccccaatgg aatccgctca tctaattttt tcccgatagt aacgacagaa
     5101 ggcatctgtc gcccggtagc ctcaagtgtt cctccgttgt tgataacttc aatagcgagc
     5161 gcggaatcat ca
//
