LOCUS       CP124769                5172 bp    DNA     linear   BCT 11-MAY-2023
DEFINITION  Klebsiella michiganensis strain 2022CK-00495 plasmid unnamed13.
ACCESSION   CP124769
VERSION     CP124769.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN31152636
KEYWORDS    .
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     2022CK-00495
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.0x
            Sequencing Technology :: Oxford Nanopore GridION; Oxford Nanopore
            MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 13:40:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,393
            CDSs (total)                      :: 7,273
            Genes (coding)                    :: 7,098
            CDSs (with protein)               :: 7,098
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 59 of 175
            Pseudo Genes (incomplete)         :: 121 of 175
            Pseudo Genes (internal stop)      :: 30 of 175
            Pseudo Genes (multiple problems)  :: 29 of 175
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00495"
                     /isolation_source="Rectal Swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="unnamed13"
                     /geo_loc_name="USA"
                     /collection_date="2022-02-25"
     gene            <1..1460
                     /locus_tag="QJQ59_05225"
     CDS             <1..1460
                     /locus_tag="QJQ59_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="WGZ97529.1"
                     /translation="DDSALAIEVINNGGTLEATGRQMPSVVTIGKKLDERIPLGDFKPS
                     YFPLWYDRNGKVIVWCKDGYPDFSGFGENAQSIVAAIPNEWAQYHLRQFDFSPSYFPLW
                     FDRKGKVLAWSKNGTVDFVIGESIQSQVKELVGANSSSSFIEGDQFKFIFKRGRIAAGV
                     AGSLNVGFGMDSWGEKSTIPQALINILGGGYKDPGYISCSTRTDGVMAGITLVVTNFTK
                     YDGDNENNNAPPPYGSGPDGNAYWNNNTVGTLTWSNVTATDLSLFYFDSTGNFTVAIDG
                     GAPVTITGTNSGTVKKYDITGLTATAHTVVVTSSGTGVVSILCMYGKNSARTNGITVSR
                     MGNGGAMAKDYLHWQDYITPIVTHFDLDLFFMILGTNDFRKSAGIAEFVNGLQVVIDKY
                     KAATPGICICLVSAAQSNATGTPTLSEYDEAMRTLAIENNVNFISLYQLFPKTYDNSGG
                     AWADALHLSDRGAYVLARKIKNEFFQE"
                     /name="GDSL-type esterase/lipase family protein"
     gene            1463..2209
                     /locus_tag="QJQ59_05230"
     CDS             1463..2209
                     /locus_tag="QJQ59_05230"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGZ97530.1"
                     /translation="MPIPAVFIDSDLPETPGMKDIDDFIVKNWFTDFPAVDGTKLGGYY
                     FGNPVSNLAMNSFNNAAPATLNGSFDNSQGYINVDVNNYLDTTLKAPVALKVLAVIRRP
                     SNPSVANYFISDFSGTGVAGLGFGLGIATDGKLRIVGQNDATTAVASVDFPSSIAVGDL
                     CAIAAHVIQGAVYVAIYNPATATYVAGNTAMSGTRSAGTKNILIGRKTDNNTSTASTAI
                     KSAVLINGEITGAQQLAAMQYLLAMD"
                     /name="hypothetical protein"
     gene            complement(2268..>5172)
                     /locus_tag="QJQ59_05235"
     CDS             complement(2268..>5172)
                     /locus_tag="QJQ59_05235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGZ97531.1"
                     /translation="DDSALAVEVINNAGTLQPTGRKMPSQAAVAANTSVITGLDLDRVD
                     VSPASLGTGQYYQLSTGTIATTTDTNWSASSPVSVAGYKRLILSGSFLYRAAVAPLLFV
                     DSAGTVISVQSLGIPMSSDNVWSSRSEVQVDIPAAAVSALISTYAEPAVAVYAAAQAFR
                     ESGVALSASKTKKRVLNWIPNTYMNIATGVLTSSSDTFAAARVPVAPGDVFTLSCSLKG
                     APNQIRLIGYFDSNETLIGTFYPGTGSGTVSTITNLTFRVPKNAASMVITAYSPNSSTV
                     LMQASVFDRAALDIQTAADTAGNLAKSSVLPFYSGRYWKNTNGALTVVNDAQFCAFYPV
                     QVTAGDVYRIKSNLTTGNPSLVSMVVFKGANGDLVGTAQPAPGSGAFAAADITVTVPAG
                     AVLMLLTAYSPYIDIVYQGSAVEKIGSSVAGIAASFTQQLSINFSKGYYWNPTSGTLTS
                     TTDPNWRAFDAIPVSPGESYRVIATEFSGSIYVVVFKNASGAVVSKSQLGPGGSSIVAV
                     DVPVTVPAGATSMCVTAYTDNVTITKMVNYRADLRKDIIQRDFDTGLRLLSPKGLDGYY
                     WHQNTGVRTLITSDWAYFSVDPITVSPGEVYRIICAEFSGNPANVYLALFKDSSGAIVG
                     RSYAGPGSGVLQAVDTNVTVPAGATSMCVTGHSRNLQIIKSGGMISKLPGNSQTQASSP
                     LDYWKGKKIVWLGTSIPAGSGTNSYPYMLAQRLGANIVNQSVGSSPIRGGLDAFVTTDD
                     PYGWTGSSYTRVTRALSHSVDIKQSFIDNYDSKWKALVTGGPASLSDTDKANILSYSYA
                     NRLSGNLDSDLFVIDHGINDYLWIQERGGDVASLLTPAVDTRNINTFYGGINTVIDYIL
                     SQNPRARILVIGFYENELRPQVSQIQLKSAQLWEYQIVKLWEKTGWSQQVLTGTDGAGK
                     TITQYWMPDNLHPHSDTTGKANTLLANILEMEIRSVR"
                     /name="hypothetical protein"
ORIGIN
        1 ctgatgattc cgcgctcgct attgaagtta tcaacaacgg aggaacactt gaggctaccg
       61 ggcgacagat gccttctgtc gttactatcg ggaaaaaatt agatgagcgg attccattgg
      121 gggatttcaa accatcatat ttcccactat ggtatgacag aaatggaaaa gtcattgtct
      181 ggtgcaaaga tggatatcca gatttctcgg gtttcgggga aaacgcacaa agcatcgtag
      241 cagccatccc taacgagtgg gcccagtatc atcttcggca gttcgacttt agtccctcgt
      301 acttccctct ctggttcgac agaaaaggca aagtcctggc gtggagtaag aacggcacgg
      361 tggattttgt cattggagag agcattcaga gtcaggttaa agaactggtt ggcgcaaatt
      421 cttcgagttc attcattgaa ggcgatcagt ttaaatttat ttttaagcgg ggtcgcatcg
      481 ctgcgggcgt agcaggcagc ctcaatgtgg gatttggtat ggattcatgg ggagaaaaga
      541 gcaccatacc gcaggcactt attaatatcc tgggtggagg atacaaagac cccgggtata
      601 tcagttgctc taccaggaca gatggggtta tggccggaat cactttggta gtcaccaact
      661 ttacgaaata tgacggtgat aatgaaaaca acaatgcacc gcctccgtac ggctcaggcc
      721 ctgacgggaa cgcctactgg aataacaata cggtagggac tctgacctgg tcgaatgtta
      781 cggccactga cctgtcatta ttttattttg acagcacggg taattttact gtcgctatcg
      841 acggtggtgc acctgtgact atcaccggaa caaatagcgg cacggtaaaa aaatacgaca
      901 ttaccggact gactgcgact gcacatactg ttgttgtaac cagttcaggg acgggtgttg
      961 tttccattct ctgtatgtat ggaaaaaata gcgccagaac caacggaatc accgtttcgc
     1021 gcatggggaa tggtggggct atggcaaaag actatttgca ctggcaggac tatattaccc
     1081 cgattgttac tcactttgac ctcgatttat tttttatgat tcttggtacc aacgatttca
     1141 ggaaaagcgc gggtatcgct gagtttgtca atgggttaca ggtagttatc gataaataca
     1201 aagcggccac gccgggaatt tgtatttgcc tggtttctgc ggcgcaaagc aacgcaaccg
     1261 gaacgcctac cttatctgaa tatgatgaag ccatgcgtac gctggcaatc gagaataacg
     1321 ttaattttat cagcctctac cagttgtttc caaagacgta cgataactcc ggtggcgcat
     1381 gggctgacgc attgcacctg tcagaccgtg gcgcatatgt actggcgcgt aaaatcaaaa
     1441 acgaattttt ccaggagtaa ttatgccgat accagctgtt ttcattgatt cagacttgcc
     1501 ggaaacgccg ggaatgaaag atattgacga ttttatcgta aaaaactggt ttacagactt
     1561 ccctgctgtc gacggaacaa aactgggtgg gtattacttc ggaaatcccg taagtaacct
     1621 ggcgatgaac tcatttaaca atgcagcacc tgcgacattg aatggatcat tcgataactc
     1681 acagggttat attaacgttg atgttaataa ctatctcgac accacactta aagcgcctgt
     1741 tgccttaaaa gtgctggcgg tcatcagacg accatcaaat ccttctgtgg caaattattt
     1801 catttctgac ttttccggta cgggagtagc ggggctcgga tttgggcttg gcattgccac
     1861 tgacggtaaa ttgcggatag ttggtcaaaa cgacgctacc acagcggtgg catcggtgga
     1921 tttcccttct tcaattgcgg tcggtgacct gtgcgcaata gcggcacacg ttattcaggg
     1981 agcggtatat gttgcaatct acaatcctgc aactgcgacg tacgtcgcag gaaataccgc
     2041 tatgtcaggt actcgttcag caggtacaaa gaatatcctt attggcagga aaacagataa
     2101 caatacgtca accgcttcaa cggcaatcaa gtctgcagtc ctgataaatg gcgagatcac
     2161 aggagctcag caacttgcgg caatgcaata cctgctggca atggattaat gtagaaagaa
     2221 atgcgttgcg gctgtcagtt ttcatgatag ccgcttggtg ttgaaagtta acgaaccgac
     2281 ctgatttcca tttcaagaat attcgccagc aatgtgtttg cctttcctgt cgtatccgag
     2341 tgagggtgca ggttgtccgg catccagtac tgggttatgg tcttccctgc gccatcagtc
     2401 ccggttaata cctgctggct ccagcctgtc ttctcccaca acttcacgat ctgatactcc
     2461 cacagctgcg cggattttaa ctgtatctga gaaacctgcg gcctcaactc attttcataa
     2521 aagccgataa caagaatgcg ggcccgtggg ttctggctca ggatgtagtc aattaccgta
     2581 tttatgccgc cgtagaaagt attgatgtta cgggtatcga ccgcaggcgt cagcagactg
     2641 gcaacatctc cccctcgctc ctgaatccac agatagtcgt taatcccgtg atcgataaca
     2701 aacaggtccg aatccaagtt acccgacagc cggttggcat aggaataact caggatattt
     2761 gccttatcag tatctgacag ggatgccggg ccacctgtca ctaacgcctt ccatttcgaa
     2821 tcgtagttgt cgataaatga ctgcttaata tcgacggaat gcgacaaggc acgcgtgacg
     2881 cgggtatatg agcttcccgt ccatccatat ggatcgtcgg tggtgacgaa ggcatccaga
     2941 ccaccacgaa tcggagatga gccgacggac tggttaacta tgttcgcccc caggcgctgg
     3001 gccagcatat acgggtagga gtttgttccg cttcctgcag gaattgaggt tcccagccag
     3061 actatttttt tccccttcca gtagtcaagc gggctgctcg cctgggtttg agagttcccc
     3121 ggaagtttgc taatcatccc tccagattta atgatctgca aattcctgct gtggccggta
     3181 acgcacatgg acgtagcgcc agccggcacg gtaacgttgg tatcaacagc ctgaaggacg
     3241 ccggagccag gtcctgcata gctgcgcccc acaatggccc cggaggagtc tttgaacagc
     3301 gcgagatata cgtttgccgg gtttccagaa aactcggcgc agatgatccg gtacacttcc
     3361 cccggagaaa ccgtgatcgg gtctacagag aaatatgccc agtcgctggt aatcagcgtc
     3421 ctgacccccg tgttctggtg ccagtaatag ccgtccagcc ctttaggaga aagcagacgc
     3481 aacccggtat caaagtcgcg ctgaataata tccttccgta aatctgccct gtagttgacc
     3541 atttttgtga tggtcacgtt atccgtatag gccgtaacgc acatggacgt agcgccagcc
     3601 ggcacggtaa cggggacatc gacagcgacg atcgaagacc caccaggacc gagctgagac
     3661 ttgcttacga ccgcaccact cgcatttttg aaaactacga cgtaaatgct accgctaaac
     3721 tcagtcgcaa tgacacgata cgactcacca ggcgaaacgg gtatggcgtc aaatgcccgc
     3781 cagttaggat cggtggtact cgttaaggtg ccggatgttg ggttccagta ataccctttc
     3841 gaaaagttaa tgctgagttg ctgagtaaaa cttgcagcga tgcccgccac cgacgatccg
     3901 attttttcta cagcgctacc ctgatagacg atgtctatat atgggctgta tgcagtcaaa
     3961 agcatcagca ccgcaccagc cgggacggtt acggtgatat ccgctgctgc aaaggccccg
     4021 gacccggggg cgggctgagc agtgcctacc agatcaccgt tggcgccctt gaaaaccacc
     4081 atcgatacca gagacgggtt ccccgtcgtc aggttagact tgatgcggta cacatcaccg
     4141 gcggtcacct gtactgggta gaaggcgcaa aactgcgcgt cgttcacaac ggtcaatgca
     4201 ccattggtgt ttttccagta gcgcccgcta tagaaaggca gcaccgacga cttcgcgagg
     4261 tttccggctg tgtcagcagc ggtctggata tcaagcgccg cacggtcgaa gacggaagcc
     4321 tgcatcagca ccgttgacga gttcggggaa tacgcggtga tcaccataga cgccgcgttt
     4381 tttggcacgc ggaaagtcag gttggtgatc gtggataccg tgccgctccc tgtccctggg
     4441 tagaacgtac cgatgagagt ctcgttactg tcaaagtaac cgatcaggcg aatctggttt
     4501 ggtgccccct tcagcgagca actgagtgtg aatacatccc ctggtgccac cggcacacgg
     4561 gcggccgcga aggtgtctga gctggatgtc agaacgcccg tcgcaatatt catgtatgtg
     4621 ttaggtatcc agttcagaac ccgttttttc gttttactgg ccgatagggc aacgcctgac
     4681 tcccggaaag cctgcgcggc tgcatagaca gccacagccg gttcggcata ggttgatatc
     4741 agtgcgctga ctgctgcggc gggaatatcg acctgaacct ctgaacgaga tgaccagaca
     4801 ttatccgagg acatgggaat gcccaggctc tgaactgaaa taacagtccc tgccgagtca
     4861 acaaacaaaa gcggtgcgac ggcagcacga tacaaaaacg aaccagacaa aattaagcgt
     4921 ttatacccag ccactgaaac gggggagctc gccgaccagt tggtgtcagt ggtggtcgcg
     4981 attgtccctg tggagagctg ataatactgg ccagtcccca gcgacgctgg agatacatca
     5041 acacgatcaa gatccaagcc tgtaattacg gatgtgttcg cggcaacagc cgcctgagat
     5101 ggcattttcc gcccggtagg ctgcagcgtc ccggcgttat tgatcacttc tacagctaac
     5161 gcgctgtcat cc
//
