LOCUS       CP123689               10967 bp    DNA     circular BCT 14-MAY-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Senftenberg strain
            FSIS11813680 plasmid pFSIS11813680-1, complete sequence.
ACCESSION   CP123689
VERSION     CP123689.1
DBLINK      BioProject: PRJNA292666
            BioSample: SAMN09954714
KEYWORDS    .
SOURCE      Salmonella enterica subsp. enterica serovar Senftenberg
  ORGANISM  Salmonella enterica subsp. enterica serovar Senftenberg
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Ge,B., Mukherjee,S., Li,C., Harrison,L.B., Hsu,C.H., Tran,T.T.,
            Whichard,J.M., Dessai,U., Singh,R., Gilbert,J.M., Strain,E.A.,
            McDermott,P.F. and Zhao,S.
  TITLE     Genomic analysis of azithromycin-resistant Salmonella from food
            animals at slaughter and processing, and retail meats, 2011-2021,
            United States
  JOURNAL   Microbiol Spectr 12 (1), e0348523 (2024)
   PUBMED   37991374
REFERENCE   2  (bases 1 to 10967)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (05-SEP-2018) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 10967)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2023) CVM/OAS, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-SEP-2021
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 257x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/20/2023 10:46:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,755
            CDSs (total)                      :: 4,635
            Genes (coding)                    :: 4,519
            CDSs (with protein)               :: 4,519
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 44 of 116
            Pseudo Genes (incomplete)         :: 83 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 25 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Salmonella enterica subsp. enterica serovar
                     Senftenberg"
                     /mol_type="genomic DNA"
                     /strain="FSIS11813680"
                     /serovar="Senftenberg"
                     /isolation_source="animal-cattle-steer"
                     /sub_species="enterica"
                     /db_xref="taxon:28150"
                     /plasmid="pFSIS11813680-1"
                     /geo_loc_name="USA:KS"
                     /collection_date="2018"
                     /collected_by="USDA-FSIS"
     gene            complement(115..399)
                     /locus_tag="D3F45_23620"
     CDS             complement(115..399)
                     /locus_tag="D3F45_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WGN64983.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            703..1236
                     /locus_tag="D3F45_23625"
                     /pseudo=""
     CDS             703..1236
                     /locus_tag="D3F45_23625"
                     /inference="COORDINATES: protein motif:HMM:NF015354.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            1346..1759
                     /locus_tag="D3F45_23630"
     CDS             1346..1759
                     /locus_tag="D3F45_23630"
                     /inference="COORDINATES: protein motif:HMM:NF036929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WGN64988.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            1804..2727
                     /locus_tag="D3F45_23635"
     CDS             1804..2727
                     /locus_tag="D3F45_23635"
                     /inference="COORDINATES: protein motif:HMM:NF028103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WGN64989.1"
                     /translation="MGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDV
                     YIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELR
                     GQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAG
                     PALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDD
                     LSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQ
                     LARAELARAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            2788..3000
                     /locus_tag="D3F45_23640"
     CDS             2788..3000
                     /locus_tag="D3F45_23640"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGN64984.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            3002..3208
                     /gene="urfF"
                     /locus_tag="D3F45_23645"
     CDS             3002..3208
                     /gene="urfF"
                     /locus_tag="D3F45_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WGN64985.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            3238..4077
                     /locus_tag="D3F45_23650"
     CDS             3238..4077
                     /locus_tag="D3F45_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WGN64986.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVNKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            4064..4915
                     /gene="repC"
                     /locus_tag="D3F45_23655"
     CDS             4064..4915
                     /gene="repC"
                     /locus_tag="D3F45_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WGN64987.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGSEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            5223..5594
                     /locus_tag="D3F45_23660"
                     /pseudo=""
     CDS             5223..5594
                     /locus_tag="D3F45_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     gene            complement(5674..6564)
                     /gene="estX"
                     /locus_tag="D3F45_23665"
     CDS             complement(5674..6564)
                     /gene="estX"
                     /locus_tag="D3F45_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold putative hydrolase EstX"
                     /protein_id="WGN64981.1"
                     /translation="MRDIQMLYVKLEGNFSMKEKVVVDKAISLYTESFGDPAHEPIILI
                     MGAMSSAVWWPDEFCSQLAKMGRYVIRYDHRDTGKSTSYEPGQAPYSVEELADDVVRVI
                     DGYGLEAAHLVGMSLGGFLSQLVALKYPKRVKSLTLIASERLADADPDMPAFDPAIIEY
                     HQRAESLDWSDRDAVVAYQVGAWRINSGTAHAFDAEKIQNIAELNFDRTPNILTTFNHT
                     TLGGGERWLGRLNEIAVPTLIIHGTEDPVLPYVHGLALKDAIRGSKMLTLEGTGHELHH
                     EDWPRIIQAIKGQTS"
                     /name="estX"
     gene            complement(6981..7142)
                     /locus_tag="D3F45_23670"
                     /pseudo=""
     CDS             complement(6981..7142)
                     /locus_tag="D3F45_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            7134..7241
                     /locus_tag="D3F45_23675"
                     /pseudo=""
     CDS             7134..7241
                     /locus_tag="D3F45_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /name="sulfonamide-resistant dihydropteroate synthase Sul2"
     gene            7328..7570
                     /gene="glmM"
                     /locus_tag="D3F45_23680"
                     /pseudo=""
     CDS             7328..7570
                     /gene="glmM"
                     /locus_tag="D3F45_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     gene            complement(7571..7682)
                     /locus_tag="D3F45_23685"
                     /pseudo=""
     CDS             complement(7571..7682)
                     /locus_tag="D3F45_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018600594.1"
                     /note="frameshifted; incomplete; partial on complete
                     genome; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0005524 - ATP binding
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /name="ATP-binding cassette domain-containing protein"
     gene            complement(7709..8614)
                     /gene="erm(42)"
                     /locus_tag="D3F45_23690"
     ARG             complement(7713..8614)
                     /name="erm(42)"
                     /gene="erm(42)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(42)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(9035..10528)
                     /locus_tag="D3F45_23695"
     CDS             complement(9035..10528)
                     /locus_tag="D3F45_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="WGN64982.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91-like element ISVsa3 family transposase"
     ARG             5224..5693
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="57.56"
     Typing          354..509
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          3256..4077
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.878"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          599..2724
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 accaaacccc gcagaaatac gctggagcgc ttttagccgc tttagcggcc tttcccccta
       61 cccgaagggt gggggcgcgt gtgcagcccc gcagggcctg tctcggtcga tcattcagcc
      121 cggctcatcc ttctggcgtg gcggcagacc gaacaaggcg cggtcgtggt cgcgttcaag
      181 gtacgcatcc attgccgcca tgagccgatc ctccggccac tcgctgctgt tcaccttggc
      241 caaaatcatg gcccccacca gcaccttgcg ccttgtttcg ttcttgcgct cttgctgctg
      301 ttcccttgcc cgtacccgct gaatttcggc attgattcgc gctcgttgtt cttcgagctt
      361 ggccagccga tccgccgcct tgttgctccc cttaaccatc ttgacacccc attgttaatg
      421 tgctgtctcg taggctatca tggaggcaca gcggcggcaa tcccgaccct actttgtagg
      481 ggagggcgca cttaccggtt tctcttcgag aaactggcct aacggccacc cttcgggcgg
      541 tgcgctctcc gagggccatt gcatggagcc gaaaagcaaa agcaacagcg aggcagcatg
      601 gcgatttatc accttacggc gaaaaccggc agcaggtcgg gcggccaatc ggccagggcc
      661 aaggccgact acatccagcg cgaaggcaag tatgcccgcg acatggatga agtcttgcac
      721 gccgaatccg ggcacatgcc ggagttcgtc gagcggcccg ccgactactg ggatgctgcc
      781 gacctgtatg aacgcgccaa tgggcggctg ttcaaggagg tcgaatttgc cctgccggtc
      841 gagctgaccc tcgaccagca gaaggcgctg gcgtccgagt tcgcccagca cctgaccggt
      901 gccgagcgcc tgccgtatac gctggccatc catgccggtg gcggcgagaa cccgcactgc
      961 cacctgatga tctccgagcg gatcaatgac ggcatcgagc ggcccgccgc tcagtggttc
     1021 aagcggtaca acggcaagac cccggagaag ggcggggcac agaagaccga agcgctcaag
     1081 cccaaggcat ggcttgagca gacccgcgag gcatgggccg accatgccaa ccgggcatta
     1141 gagcgggctg gccacgacgc ccgcattgac cacagaacac ttgaggcgca gggcatcgag
     1201 cgcctgcccg gtgttcacct ggggccgaac gtggtggaga tggaaggccg gggcatccgc
     1261 accgaccggg cagacgtggc cctgaacatc gacaccgcca acgcccagat catcgactta
     1321 caggaatacc gggaggcaat agaccatgaa cgcaatcgac agagtgaaga aatccagagg
     1381 catcaacgag ttagcggagc agatcgaacc gctggcccag agcatggcga cactggccga
     1441 cgaagcccgg caggtcatga gccagaccca gcaggccagc gaggcgcagg cggcggagtg
     1501 gctgaaagcc cagcgccaga caggggcggc atgggtggag ctggccaaag agttgcggga
     1561 ggtagccgcc gaggtgagca gcgccgcgca gagcgcccgg agcgcgtcgc gggggtggca
     1621 ctggaagcta tggctaaccg tgatgctggc ttccatgatg cctacggtgg tgctgctgat
     1681 cgcatcgttg ctcttgctcg acctgacgcc actgacaacc gaggacggct cgatctggct
     1741 gcgcttggtg gcccgatgaa gaacgacagg actttgcagg ccataggccg acagctcaag
     1801 gccatgggct gtgagcgctt cgatatcggc gtcagggacg ccaccaccgg ccagatgatg
     1861 aaccgggaat ggtcagccgc cgaagtgctc cagaacacgc catggctcaa gcggatgaat
     1921 gcccagggca atgacgtgta tatcaggccc gccgagcagg agcggcatgg tctggtgctg
     1981 gtggacgacc tcagcgagtt tgacctggat gacatgaaag ccgagggccg ggagcctgcc
     2041 ctggtagtgg aaaccagccc gaagaactat caggcatggg tcaaggtggc cgacgccgca
     2101 ggcggtgaac ttcgggggca gattgcccgg acgctggcca gcgagtacga cgccgacccg
     2161 gccagcgccg acagccgcca ctatggccgc ttggcgggct tcaccaaccg caaggacaag
     2221 cacaccaccc gcgccggtta tcagccgtgg gtgctgctgc gtgaatccaa gggcaagacc
     2281 gccaccgctg gcccggcgct ggtgcagcag gctggccagc agatcgagca ggcccagcgg
     2341 cagcaggaga aggcccgcag gctggccagc ctcgaactgc ccgagcggca gcttagccgc
     2401 caccggcgca cggcgctgga cgagtaccgc agcgagatgg ccgggctggt caagcgcttc
     2461 ggtgatgacc tcagcaagtg cgactttatc gccgcgcaga agctggccag ccggggccgc
     2521 agtgccgagg aaatcggcaa ggccatggcc gaggccagcc cagcgctggc agagcgcaag
     2581 cccggccacg aagcggatta catcgagcgc accgtcagca aggtcatggg tctgcccagc
     2641 gtccagcttg cgcgggccga gctggcacgg gcaccggcac cccgccagcg aggcatggac
     2701 aggggcgggc cagatttcag catgtagtgc ttgcgttggt actcacgcct gttatactat
     2761 gagtactcac gcacagaagg gggttttatg gaatacgaaa aaagcgcttc agggtcggtc
     2821 tacctgatca aaagtgacaa gggctattgg ttgcccggtg gctttggtta tacgtcaaac
     2881 aaggccgagg ctggccgctt ttcagtcgct gatatggcca gccttaacct tgacggctgc
     2941 accttgtcct tgttccgcga agacaagcct ttcggccccg gcaagtttct cggtgactga
     3001 tatgaaagac caaaaggaca agcagaccgg cgacctgctg gccagccctg acgctgtacg
     3061 ccaagcgcga tatgccgagc gcatgaaggc caaagggatg cgtcagcgca agttctggct
     3121 gaccgacgac gaatacgagg cgctgcgcga gtgcctggaa gaactcagag cggcgcaggg
     3181 cgggggtagt gaccccgcca gcgcctaacc accaactgcc tgcaaaggag gcaatcaatg
     3241 gctacccata agcctatcaa tattctggag gcgttcgcag cagcgccgcc accgctggac
     3301 tacgttttgc ccaacatggt ggccggtacg gtcggggcgc tggtgtcgcc cggtggtgcc
     3361 ggtaaatcca tgctggccct gcaactggcc gcacagattg caggcgggcc ggatctgctg
     3421 gaggtgggcg aactgcccac cggcccggtg atctacctgc ccgccgaaga cccgcccacc
     3481 gccattcatc accgcctgca cgcccttggg gcgcacctca gcgccgagga acggcaagcc
     3541 gtggctgacg gcctgctgat ccagccgctg atcggcagcc tgcccaacat catggccccg
     3601 gagtggttcg acggcctcaa gcgcgccgcc gagggccgcc gcctgatggt gctggacacg
     3661 ctgcgccggt tccacatcga ggaagaaaac gccagcggcc ccatggccca ggtcatcggt
     3721 cgcatggagg ccatcgccgc cgataccggg tgctctatcg tgttcctgca ccatgccagc
     3781 aagggcgcgg ccatgatggg cgcaggcgac cagcagcagg ccagccgggg cagctcggta
     3841 ctggtcgata acatccgctg gcagtcctac ctgtcgagca tgaccagcgc cgaggccgag
     3901 gaatggggtg tggacgacga ccagcgccgg ttcttcgtcc gcttcggtgt gaacaaggcc
     3961 aactatggcg caccgttcgc tgatcggtgg ttcaggcggc atgacggcgg ggtgctcaag
     4021 cccgccgtgc tggagaggca gcgcaagagc aagggggtgc cccgtggtga agcctaagaa
     4081 caagcacagc ctcagccacg tccggcacga cccggcgcac tgtctggccc ccggcctgtt
     4141 ccgtgccctc aagcggggcg agcgcaagcg cagcaagctg gacgtgacgt atgactacgg
     4201 cgacggcaag cggatcgagt tcagcggccc ggagccgctg ggcgctgatg atctgcgcat
     4261 cctgcaaggg ctggtggcca tggctgggcc taatggccta gtgcttggct cggaacccaa
     4321 gaccgaaggc ggacggcagc tccggctgtt cctggaaccc aagtgggagg ccgtcaccgc
     4381 tgatgccatg gtggtcaaag gtagctatcg ggcgctggca aaggaaatcg gggcagaggt
     4441 cgatagtggt ggggcgctca agcacataca ggactgcatc gagcgccttt ggaaggtatc
     4501 catcatcgcc cagaatggcc gcaagcggca ggggtttcgg ctgctgtcgg agtacgccag
     4561 cgacgaggcg gacgggcgcc tgtacgtggc cctgaacccc ttgatcgcgc aggccgtcat
     4621 gggtggcggc cagcatgtgc gcatcagcat ggacgaggtg cgggcgctgg acagcgaaac
     4681 cgcccgcctg ctgcaccagc ggctgtgtgg ctggatcgac cccggcaaaa ccggcaaggc
     4741 ttccatagat accttgtgcg gctatgtctg gccgtcagag gccagtggtt cgaccatgcg
     4801 caagcgccgc cagcgggtgc gcgaggcgtt gccggagctg gtcgcgctgg gctggacggt
     4861 aaccgagttc gcggcgggca agtacgacat cacccggccc aaggcggcag gctgaccccc
     4921 cccactctat tgtaaacaag acatttttat cttttatatt caatggctta ttttcctgct
     4981 aattggtaat accatgaaaa ataccatgct cagaaaaggc ttaacaatat tttgaaaaat
     5041 tgcctactga gcgctgccgc acagctccat aggccgcttt cctggctttg cttccagatg
     5101 tatgctattc tgctcctgca gctaatggag caccgcaaac aggttactcg cctggggatt
     5161 ccctttcgac ccgagcatcc gtatgagact catgctcgat tattattatt atagaagccc
     5221 ccatgaataa atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg
     5281 atggaggccg gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg
     5341 agggggcaga tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt
     5401 cgtccgacac agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca
     5461 ttcccgtctc gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg
     5521 tggcctatct caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga
     5581 aatcatctgc caaacgcctt gctcaccgga aaattgcgag cgcagcgagt aatttttacg
     5641 gtgcagcaac ttgttgtacg gtgcaaccga tagctatgac gtttgcccct taatcgcctg
     5701 gataatcctc ggccagtctt catgatgcaa ctcatgtccc gtgccttcga gtgtcagcat
     5761 ttttgaacca cgaatcgcat ctttcagtgc caacccatgc acataaggaa gtacaggatc
     5821 ctccgtgccg tgaatgatca aagttggtac agctatctca tttaatctcc cgagccatct
     5881 ctcgccacca cctaaagtag tgtggttgaa tgttgtcagg atattcggag tgcgatcaaa
     5941 atttaactca gcgatatttt gtatcttctc agcgtcaaaa gcatgcgcag tacctgagtt
     6001 gattcgccac gctccgacct gatacgcgac gacagcatct ctatcagacc aatccagcga
     6061 ttccgcccgt tggtgatact caatgatggc aggatcaaaa gcgggcatat caggatctgc
     6121 atctgcaagc cgttctgaag caatcagcgt caagctcttc acacgtttcg gatacttgag
     6181 agctacaagc tgggaaagaa atccccccaa agacatgccg actaaatgag cagcttccag
     6241 accataacca tcaatgacgc gaaccacatc atctgctaat tcttcaacgg aatatggagc
     6301 ttgacctggc tcatagcttg ttgatttccc ggtatcacgg tggtcgtacc ggatcacata
     6361 gcgacccatt ttggctagtt gggagcaaaa ctcatcaggc caccacaccg cagacgacat
     6421 tgcccccatg atcaggataa tgggttcatg ggccgggtcg ccgaatgatt cggtatagag
     6481 tgaaatcgct ttatcaacaa ctaccttttc tttcatacta aaattgcctt cgagttttac
     6541 gtacaacatt tgaatatcgc gcatgcgcgt tttccgcctc atatttccca tcaagatgta
     6601 ttcgtaatta attaatatcc ttagtgaaat tcctgaatcc gaagaaagtc acaaggacgc
     6661 aaacgtgcat gcgcgatata cttctatcat tgcacgttat ccttaaaggt ccgatcttaa
     6721 actgttggca gcaacagtat ttttaacgta taggaagaat aaacgccctt ttcacccaag
     6781 tccaacagct ttggaccgca gttgactctt tcgacacccc tgcgatgcaa cccaatccgg
     6841 ctgacgggga gccagcaacg ctgaaaattt accctcctct ttcccactag cggctccttt
     6901 tccgacaacc agcacggcgg atccctgccg cggcgctgtg aacgcagcat tttgattggt
     6961 atcgttggcc ttcaggctcg tcagtcaaac agacccagga gcagctcagc cggtggcgcc
     7021 cggctttcgg gtaacgccct ggtcccgctg gtttcggctt tgtgcttcag gtgatcaagg
     7081 atctgcttga tcattatagg gtcttcaatg caggcgatga ctttcatggc gccgccgcag
     7141 ccgcaggtgg agctgacttc atccgcacac acgagccgcg ccccttgcgc gacgggctgg
     7201 cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt cgggatattt
     7261 ctctatattc gcggttcagc aggcatgtcc cctttgaggg cgacccgacg acaggataat
     7321 cgaccttatg gtgcgcaaat atttcggcac agacggtatt cgtggcaaag ccaacgaagg
     7381 cgcgatgacg gcggaaaccg ccttgcgcgt cggcatggcg gctggccgtg tctttcgtcg
     7441 cggtgaccac cgccatcgtg tcgtgatcgg caaggatacg cgcctgtcgg gctatatgct
     7501 tgaacccgcg ctcacagccg gtttcacctc gatgggcatg gacgtattcc tttttggccc
     7561 gctgccgaca acgtagaaca gttgttttcc cgcacccaga tgggccaact aaaacaacaa
     7621 aatcctcatt attaacttcc aacgaaagtt ttgtttatgt tttctagctt taattttaac
     7681 atttagcttt tccttatctg ccgtttatct attttctgtt attttgcttt ctatttctat
     7741 gaatcttttc taaactagac tcatgttcta acattctttt atacgaattg ttaactaaaa
     7801 cttttttatc cttgcttacc atgttcgaat tatagagttt aaacattttt agccaaaatt
     7861 cataactcca attacttata tttgaatcat cattaattcg gctttctttt aaaattattt
     7921 tttgttgctt ataactaaaa attcgttttg ttttttcttt aaatgtaact cccttttcta
     7981 aaaagacaaa ggctaaaaaa tctttccaag catgcctgtc ttcaaggttt atatctgtaa
     8041 agtctttata agaaaattgg cccaaaataa tgtttgcgtt tggagctggc ttaaaatcaa
     8101 atttgctaaa gctatgcaat atgttagttt tgaaaaatgg tttatataat aaagatttat
     8161 aagactcctt gtaagatggt gcacccgcat actttaaaaa aggttcatat tgcataatta
     8221 aaaaagtatc taagggtggg ttctcactat ctaacaattt atttaaaata cttgccgtta
     8281 tgttaaatgg aatgtttgaa aaaactttat aattatggtc tggtaaattg tattttaaaa
     8341 aatcggcttc aataatagac acatttgaac ttttaaattc atggctcaac ttatcagcca
     8401 atacactatc gaactcaata gcattaacgg ctttgcagat tttgcttaat gccttggtta
     8461 ttatgccttt tccaggccca atctcaacaa caaaatcctc cttagttata ttgcttttaa
     8521 aaagcaaatc ttcgactaat ttagtattat gcaaaaaatt ctgtgagtct ttaatgttga
     8581 aatttttgtt ttttatttta gtgtttttat tcatatccat ataattacaa ttatagactg
     8641 atttggtcaa gtaagaattg ttttgattta tgtataattc ctacaagttc ggaaatttaa
     8701 aaatgaaaga agcctgaaat tatcaggctt caagcttgta agatttttaa gattatattt
     8761 acactgttag aatattctct actgacatga gaaaaaccaa cgtataggaa gaataaacgc
     8821 ccttttcacc caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat
     8881 gcaacccaat ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca
     8941 ctagcggctc cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca
     9001 gcattttgat tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct
     9061 cagccggtgg cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct
     9121 tcaggtgatc aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca
     9181 tggcgccgcc gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt
     9241 gcgcccatgt catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga
     9301 ccttgttgcc cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg
     9361 cgaacacccc gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc
     9421 ttgcaatgaa atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct
     9481 tgagctggta gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg
     9541 ggcggctgat gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca
     9601 cgccggcgtg caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac
     9661 tggtcggcag agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac
     9721 ggtaagtgat cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg
     9781 ccagatagct gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca
     9841 cccggtgggc gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc
     9901 agcggaaacg cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt
     9961 ggaagtgaac attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg
    10021 tcttggccac ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt
    10081 aaacgatgcc cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct
    10141 gaaacgggaa gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca
    10201 gcaaggcggc actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct
    10261 tacagctgaa agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc
    10321 catgctccag ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca
    10381 attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct
    10441 gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc
    10501 tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata
    10561 cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga
    10621 cattgccagc cagtgcccgt cactcccgta ctaactgtca cgaacccctg caataactgt
    10681 cacgcccccc tgcaataact gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg
    10741 caaacccagc aggggcgggg gctggcgggg tgttggaaaa atccatccat gattatctaa
    10801 gaataatcca ctaggcgcgg ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat
    10861 atgcccggcc agaggccgga tagctggtct attcgctgcg ctaggctaca caccgcccca
    10921 ccgctgcgcg gcagggggaa aggcgggcaa agcccgctaa accccac
//
