LOCUS       CP123644               10384 bp    DNA     circular BCT 14-JUN-2023
DEFINITION  Salmonella enterica strain 21OH06GT03-S1 plasmid p21OH06GT03-S1-1,
            complete sequence.
ACCESSION   CP123644
VERSION     CP123644.1
DBLINK      BioProject: PRJNA292661
            BioSample: SAMN23009289
KEYWORDS    .
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Ge,B.
  TITLE     Genomic Analysis of Azithromycin-Resistant Salmonella from Food
            Animals and Retail Meats, 2015-2021, United States
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10384)
  CONSRTM   NARMS: The National Antimicrobial Resistance Monitoring System
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2020) Center for Veterinary Medicine, US Food and
            Drug Administration, 8401 Muirkirk Rd, Laurel, MD, USA
REFERENCE   3  (bases 1 to 10384)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-MAR-2023) CVM/OAS, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-SEP-2021
            Assembly Method                   :: HGAP v. 4
            Assembly Name                     :: Microbial assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 198x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/20/2023 10:06:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,552
            CDSs (total)                      :: 4,431
            Genes (coding)                    :: 4,298
            CDSs (with protein)               :: 4,298
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 17
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 60 of 133
            Pseudo Genes (incomplete)         :: 90 of 133
            Pseudo Genes (internal stop)      :: 30 of 133
            Pseudo Genes (multiple problems)  :: 42 of 133
            Pseudo Genes (short protein)      :: 1 of 133
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10384
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="21OH06GT03-S1"
                     /isolation_source="ground turkey"
                     /db_xref="taxon:28901"
                     /plasmid="p21OH06GT03-S1-1"
                     /geo_loc_name="USA:OH"
                     /collection_date="2021-06-04"
                     /collected_by="The Ohio State University"
     gene            526..1152
                     /locus_tag="LNS16_22660"
     CDS             526..1152
                     /locus_tag="LNS16_22660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGM94996.1"
                     /translation="MSPTLNKQASKERCAVEFATRKKSKTQRKKSKALSYRGLCFFCTL
                     VTGKCQWTSKMSKQDYIQLRTLTDQFYADNKGLQEALDGSNDGKVRGYGIVVIDLNGLV
                     FGIPLRSHLNHKFGFVSERSEGVKKGLDYTKALLIKKEEYVSRAYKIPTPEFTHINDNK
                     EKIQEDFNKFVNRYIEANVKKDENILRNYRYSTLKNYHKELGLED"
                     /name="hypothetical protein"
     gene            complement(1181..1744)
                     /locus_tag="LNS16_22665"
     CDS             complement(1181..1744)
                     /locus_tag="LNS16_22665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGM94997.1"
                     /translation="MHALSILPSVARANLATKFSSHLKVIELFNTMQDSQVVVLSSLIE
                     GHHLTSSGNSVKADFEVTRLPAIIEMLEKKYFFPIRHLNVSVKSVTTGRMTGQTVYFIE
                     PEHIEQLLADPELVFANQERSLFFRSLEKEGKNLGKLIEKKGSVSQAVLSLLHHAYKDK
                     PLSDEMWKEIEDKFTHMLDELSAA"
                     /name="hypothetical protein"
     gene            complement(2002..2412)
                     /locus_tag="LNS16_22670"
     CDS             complement(2002..2412)
                     /locus_tag="LNS16_22670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGM94998.1"
                     /translation="MAFISLSNLAAKTGRIIQSQCVTATQTKKSTGAVELGFRLSKSAM
                     DETGITIGDRVDILYDKEENQWMIKKISEGGFGVSGQKNKHGGYSSCAVRLTLRPGFPR
                     LSEDEEDNKKLLSDDSFTQLSEGVIVFRLDDE"
                     /name="hypothetical protein"
     gene            complement(2748..5753)
                     /locus_tag="LNS16_22675"
     CDS             complement(2748..5753)
                     /locus_tag="LNS16_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WGM94999.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGALGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            5917..6474
                     /locus_tag="LNS16_22680"
     CDS             5917..6474
                     /locus_tag="LNS16_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0000150 - DNA strand exchange activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WGM94991.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            6657..7517
                     /locus_tag="LNS16_22685"
     CDS             6657..7517
                     /locus_tag="LNS16_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WGM94992.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(8188..8400)
                     /locus_tag="LNS16_22690"
     CDS             complement(8188..8400)
                     /locus_tag="LNS16_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WGM94993.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(8404..8889)
                     /locus_tag="LNS16_22695"
     CDS             complement(8404..8889)
                     /locus_tag="LNS16_22695"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WGM94994.1"
                     /translation="MSSLLALAKDLEKQSKAQQQNTCEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEQKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(8924..9358)
                     /locus_tag="LNS16_22700"
                     /pseudo=""
     CDS             complement(8924..9358)
                     /locus_tag="LNS16_22700"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(9567..9887)
                     /locus_tag="LNS16_22705"
     CDS             complement(9567..9887)
                     /locus_tag="LNS16_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WGM94995.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGRLPSI
                     SPALLRQLAGMGNNLNQIARKVNTGGAGHDRVQIVAALMAIDAGLERLRHAVLEKGGND
                     DR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             6658..7514
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(9996..10175)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.339"
                     /coverage="83.0"
                     /evalue="7.539999999999999e-48"
     Typing          2..425
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.65"
                     /coverage="91.0"
                     /evalue="6.42e-122"
     Typing          10121..10384
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.698"
                     /coverage="99.0"
                     /evalue="1.4000000000000001e-98"
     Typing          complement(8073..9577)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.426"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
       61 tccgctgtta tggccgcgtt tatctcattc cacgcctgac actcagttcc gggtaggcag
      121 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgcctttt
      181 ccggtaacta tcgacttgag tccaacccgg aaagacacga ctaatcgcca ttggcaacgg
      241 ccactggtaa acatgatgtg cgagagatgt attgagagtt cttgaagtgg tggccttact
      301 gaggctacac tggaaggacg gatttggtga cacgtctcgc acaagactgt taccacggtt
      361 agataatctg ccaggattaa ataaccttcg aaaaaccacc tgccagggtg gttttttcgt
      421 ttcaggagaa gattacgctg gcaaagtaag tccagtcaag aacagctacg aaattgactt
      481 cacatataat ttttgatata agtaaatcta gaagtggcgg taggaatgag ccccacgtta
      541 aacaaacaag cttccaaaga aagatgtgcg gtagagttcg ccacacgtaa aaaatccaag
      601 actcaacgaa agaaaagcaa agccctctct tacagagggc tttgcttttt ttgtacattg
      661 gttacaggaa aatgccaatg gacatcaaaa atgagcaaac aggattacat acagttaaga
      721 acccttacag accagtttta tgctgataac aaaggcctac aagaggctct cgacggcagt
      781 aatgatggaa aagtaagagg ctacggaatc gtagttattg atctcaacgg attggtgttc
      841 gggataccct tacgaagcca tttaaaccat aaatttggat tcgtttcgga gcgatcagaa
      901 ggtgttaaga aaggactgga ttacaccaaa gcgttgctaa ttaagaaaga agagtatgta
      961 tcgcgagcct ataagatacc aactcctgaa tttacccata tcaatgataa caaagaaaaa
     1021 atacaagaag atttcaacaa attcgtaaac agatacatag aagcgaacgt caagaaagat
     1081 gaaaacatac tcagaaacta tcgttactct acgctgaaaa actatcacaa agaattaggt
     1141 ttagaagact aaaaaggcgg tctaacgacc gcccttgcct ttacgccgcg ctcaattcat
     1201 ccagcatatg ggtgaactta tcttctatct ccttccacat ctcgtcagaa agaggtttat
     1261 ctttgtaggc gtgatgtaga aggcttaaga cggcttgaga aacactacct tttttctcga
     1321 taagcttgcc gagatttttc ccttcttttt cgagagaacg aaagaagagg ctcctttcct
     1381 gattagcaaa cacaagctca ggatcagcga gaagttgctc gatgtgttca ggctctatga
     1441 aatagacagt ttgtccagtc atccttcctg tagttacaga tttaacagat acatttagat
     1501 gacgaatagg gaaaaaatat tttttctcta acatctcgat aatcgccggt aagcgagtga
     1561 cctcaaagtc ggctttgaca ctgttaccag aactggtgag gtgatggcct tcgatcagtg
     1621 atgacaagac aacaacttgg gaatcctgca tcgtgttgaa gagctcgatc accttcaaat
     1681 gagacgagaa tttagttgcc aggttagcgc gggcaactga aggtagtatt gataaagcgt
     1741 gcatatgtat agcctcatat gtttttaaaa acataaactt aagagaccaa agccaatagc
     1801 atcgcaccta tcagaataga aaatgtcgtg ttcacatccc tcaaaagaaa cgattttttc
     1861 tttattagaa tgcaagttta tgaacataat gccgcccata acactccacc cttccaaagg
     1921 tagtgatgtg ttgacaagaa ggcggagtta gtcgattgtt tggcgacgct cactaactct
     1981 gcctttcctt tttggtcctt cctattcatc atccagcctg aagacgatta caccttcact
     2041 tagctgagtg aatgaatcat cggaaagtag cttcttgttg tcctcttcat cctcagaaag
     2101 gcgaggaaaa cctggtcgca aggtcaatct aacagcacac gatgagtaac cgccgtgctt
     2161 gtttttttgc ccagacacac caaagccacc ttcactaatt ttcttaatca tccactggtt
     2221 ttcttctttg tcgtaaagaa tgtcgacacg atccccaatg gtaataccag tctcgtccat
     2281 agcgctcttg ctcaagcgga atcccagctc gacagcgccc gtgctttttt tcgtttgcgt
     2341 ggccgtaaca cactgcgact ggattatcct tccagtcttc gctgccaagt tcgatagaga
     2401 gataaaagcc ataatacaat ccgttttaga catcgatcag ttcttatacg gaagtgtacc
     2461 cagggagcga ggtggctttc aagatctttt gtattgattt ttctagagcc gcaaaaaacg
     2521 gcgataagaa accttaaata taaaaacaat tgctcgtatt gaacctcgtc aagatacaca
     2581 tccagatcga tctttatgta agtaaagggt atttttctat ggtgatttaa agtcgagcaa
     2641 cgtcagcgca tccaaaaaat caccaagtag gtgcaagaaa gccgcttata gaaactgatt
     2701 tttatgcctg tataggggtc tgacgctcag tggaacgaaa actcacgtta agcaacgttt
     2761 tctgcctctg acgcctcttt taatggtctc agatgtcctt tggtcaccag ttctgccagc
     2821 gtgaaggaat aatggccgag catattgata tgtccgtggc aaagcgggga gaggcgtgcg
     2881 atatcttcat cattcagtgt ttcaccctgc gcccggagat gatccagggc tgcctgcata
     2941 taaatagtgt tccataacac gacggcgtta gtgaccagcc ccagtgcgcc cagttgatct
     3001 tcctgaccgt cggtatatcg ttttcttatc tcaccttttt gaccgtgaca gatggctctg
     3061 gcaacggcat ggcgactttc tccccgatta agctgggtca gaatgcgccg gcggtaatct
     3121 tcatcatcaa tataattaag cagatacagc gttttgttga tgcgccccac ttcaatgatt
     3181 gcctgagtca gtccggaagg acgttcactt ttcagcaatg aacggaccag cactgaagcc
     3241 tgtactttgc ccagcttcag ggagccagcg gtccggatca tttcgtccca ctgaaggact
     3301 atttttcggg gatctgattg ccctctggca atatcattca gcacgccata gtcggcatca
     3361 tggtccattc gccagaaaac cgaagcaccg gcatcagcca ggcgtggaga aaactggtat
     3421 cccagcagcc agaaaaggcc aaagacaagt tcgctggcac ctgctgtatc ggtcataatt
     3481 tcggttggat tcagcccggt ctcctgttcc agaagacctt ccagcacaaa gatagagtcc
     3541 ctcagcgtcc ccggtataac gatgccatga aagccggaat actgatcgga cacaaagttg
     3601 taccaggtga tccctctgtt attaccaaag tatttgcggt tcggtccggc attgattgtt
     3661 ctgactggcg taacaaagcg cattccatct gcagatgcca cttctcctcc accccatatc
     3721 tgtgccagtg gcagcgttgc ctgaaaatca accagtctgg cattagcgct ggtgatagtt
     3781 tcagcccgca gatagttcgc ttttgtccag ttcagccggt gtcgggtcag tgcaggaaca
     3841 tttgatctga tcagtggttc cagaccgata ttgcaggctt cagccatcag cacggcgctg
     3901 atgctgacgg gcagatcatc aactctggca ctggcttcac tagcatggaa aaactcatca
     3961 gcaaatccgg tatgggcgtt aatttcgagc agcaactccg ttaaatccac cggagggagt
     4021 agatcactga tcattttgct cagtcgtttc agactgtccg gctcatcaag actggcgagg
     4081 ggagaaattg tcaaccgggg cttcgggcca gaaacatcga gttcgacagc ctcattttcg
     4141 caaagacgtg cagcaacctg tctgtaacga ctatcaagct gatgacccag agattttatt
     4201 gcttcctgcg ggtctgtcgg gtgccccaaa gaacgataaa ccttaatccg gtttgcctgc
     4261 cagtcagcac cctgtagtaa tcttgcacga ggatctcccc accggttact gccggtaacg
     4321 tagacatccc tccgcctcag actatcctgc agtttactga gaaagcagag cgtgtatccc
     4381 ctgcgggtga tatgtttttc cttgttaatc accagccgtt tccatgaccg actgataatt
     4441 tccgttggtg cgtcgtcaaa aaactgccgc cgtgagctga actcccggct gaggtagtca
     4501 caggcattca gagtggtaac cccggcaggt gcggatgaaa atttaacggt attcagcaga
     4561 tggggcagga aacgacgaac gcgcccgtac tgctccacca tttcttcatg aaaattatcg
     4621 tctgagggcc gggcaatttc acggacaagc gtgatgattt cagccagctt ttgccttggg
     4681 atgtagctga acacctcagc acgaatcgat tcgtccggtg tttcttcttt cagcaggtac
     4741 gaacatgcgc tggcgagcgc caatgcagat ttatccagat ccttcagcga gcggagccgt
     4801 tttttctgcc caatctttct ggcgtcacgg atgataacgg ccagcatggc gtccagaacg
     4861 tccaatgcat catccagcgc cagcgtttcc catgcaagga caaaggcaac cagaaccgcc
     4921 atccttttct gcggtgacat cctggcaata ttgaacaccg aagtcatacc agcataacgt
     4981 gcgagatttt tcaggcgcac agccgggagt gtactcaggt tttcagcatg caggccaaaa
     5041 tcgttcagag ttttccagcg ttcaattgct tcattaaacg ccggaccact gatggtcaca
     5101 gggccctttt tcagtgattc cagtaaagac aggcggctgc aatcagttgg ccccagcagc
     5161 atctccagct gtgaacgctg ttcggctgac ggtatcagtg ccagtttgtt ccacaggcgc
     5221 aacgtcgcct tttcccttac ctctgaaatc aaccgggtca gcgtagtggc tccggggaga
     5281 ataatacgat gttgcataag ccaccctgtc gccagatcga aaagcaggcc aggacgttcg
     5341 ttgcttatcc agctccgggt atataaaaga cgggtaaggc gaaatgtcca gggccaggca
     5401 aattcacgat actgatagtg ctgacgtatc agcgctgcat gctcacggcg ggtattttcc
     5461 ctctgaccgt attctgcaag aacggtgata tcacgaatcc cgagctgtct ggcggtaaaa
     5521 tgccggacgc cggaaggaat atgattcata tcggtgagga aggtgcccag aaaacggaca
     5581 catccaattt gcagggcaat gcccagacgg ttgtgatcac ctctgctttt tccgataaat
     5641 tccttgtctg cttcatcaag gtgaaaatat cgtgccagct gaagctcatc cggttcaccg
     5701 gtgaatctgc catagctttc agtctgctca gtggtcagaa agtcaacggg catatcggcc
     5761 tccctgcctg acgggcattt agtaacattt ttgcaaccgt acgaaatgtt ataaattatc
     5821 ggacatcgta aaactgttac attaatatgt ctattaaatc gtaaatttgt aataatagac
     5881 atgagttgtc cgatattcga tttaaggtac atttttatgc gactttttgg ttacgctcgg
     5941 gtctcaacca gtcagcagtc tcttgatctt caggtcagag cactcaaaga cgcaggtgtg
     6001 aaagcaaacc gtatatttac cgataaggca tccggcagtt caacagaccg ggaagggctg
     6061 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
     6121 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
     6181 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
     6241 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
     6301 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
     6361 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
     6421 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
     6481 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     6541 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6601 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6661 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6721 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6781 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6841 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
     6901 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6961 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7021 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7081 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7141 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7201 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7261 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7321 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7381 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7441 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7501 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7621 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtatag gttgccgatg
     7681 agatgaattt aacataagag ttcttgcccg caccgcttcg ggctggttcg gcgcaaatac
     7741 ggtgcctgtc gggtgaaacc cctagcaaag cacccggtaa accgccccgt ttacgccaga
     7801 taaatttcac ttggctgcat ggttatgcag ccagatgaat gacagcagtt ttcgcgtata
     7861 aatacggtgt aaagcactaa aaaggccact ttcccccgaa tcgctcccac agatccattg
     7921 ccagtttgac gcattgcagt atcagactga gcgcttttag tatcttcata aatccccctc
     7981 tcttgagccg tgatcttttc atcacgaccg tatttagcgg gaaatttatg agcccttctt
     8041 cttggggttt gtcactggat gcgcgcagtc agcccagcgt cggaccgtta tagctccgct
     8101 gacggaaacg gggttctgcc agctcttttt catgctgtcg ctgctcgtat gcccgttcgg
     8161 cggccaccgc catttcatgc cgctgcacta ctggctcaaa cgctgtaact gcctgctcaa
     8221 gttcgccacc tgctgactca agcgcgtgac gcgctcgttc agcgccgcgt tctcccgttg
     8281 cataagacca gacatcttct gccattccgc gaaggcgctc tcccactcgt ccagcctttg
     8341 cgagtagtcc tgatgtagct gctctaatgc ggtcagcaac tgtttttcca gttccgtcat
     8401 gggctatttc ctctccagta ccatccagtt cccttcctcc ccgtaagctc ccgctttcgg
     8461 gttcactttg acgcaggaca gtttctgttc cccacagagc gacagctgca cgccgtggtt
     8521 cttctccgac agcatggcct gcgtccgctc ctgcgcccgg atagtctggt aattcccggt
     8581 gatctgctgc ccctgccacc acagaatgct cccgctcgtg gcgatcagca gtactgagac
     8641 cagggtgatg gtcagccacg tccggccaac catacgcagc acgctcagcc ggttcgactg
     8701 catcgccgcg gtcataccct gctcgtgggc gctgatggcg tcgctgattc tcttcgcgct
     8761 tgcgctcagt tccgctttta cagacttctc gtgctcgctg aatgcggctt tcagcatctc
     8821 gcaggtattc tgctgctgcg ctttcgattg cttttccaag tccttcgcca gcgctaaaag
     8881 actgctcata aatggctccc ttcagtcgga tgtttcgccc cccgtccggg tcggcaatgc
     8941 tgatactgtt ctttgtggtg cggaccacct caaaacctgc cgcagtgagc gaccctgtga
     9001 cgtcctcgcg cgttttgagc gccccggatg aggctaaggc cagcaaaccc cgtgtaatcg
     9061 cctgagcggc ttccagcttc gtttccggca gtgcggacgg cgtgaccagt aaccgccggt
     9121 tcttcggggc gtttggatca tgcagcccca gtctgccgtt caccacggtc tgccaggcat
     9181 cgatgcgcgg ccggtcggcg cgatcgtaat acggctgcag gcgtttcccg gtcagcagct
     9241 ctgtgttggg gatcaggaaa ttcagctcca gccgcccctt gtcggtgtgc tccacccaca
     9301 gaatgctgta ctggtcttta tcgagcccgg gcatcagaac ccgctcaaag ctggccatga
     9361 tctgctcccg ctgcccgggc ggcagctcag cctccgcgaa cgacagtacg ccggaggtgt
     9421 actttttcgc atacggcgag gcatcgataa gctcccggac ttcctccggc tttccctgca
     9481 gcacggtcgc cccctcgcgc tgccggtctt ttcccagcag gtaatccacc ggaccggcac
     9541 caccaccgcg cccccgggga tggaatttaa cgatcatcat tccccccttt ttccagcacc
     9601 gcatgccgca gccgctccag tcccgcgtcg attgccatca gggcggccac gatctgcacc
     9661 cggtcatgac cggcaccacc ggtattcact ttccgggcga tctggttcag gttattaccc
     9721 ataccggcaa gctggcgcag cagcgccggc gatatcgacg gcagtctgcc ggcacgcgcc
     9781 ggcttctcat cgaggcaggt ctgacgcatc cacgccgcca gctgcttgcc gtcgcaccgc
     9841 tcgagcagcc gccggtgttc gtcttccgtc acccacatcg tgagcatctt gttgcgtttg
     9901 tctgccagag tagcctcctg ataacaccgg gacgggcggg aaatgtttcc cggtcggcac
     9961 ggtgttggac aagccgcagg cgcgtcagtg gcttttagcg ggtttcgggg cgcagccctg
    10021 aaccagtcac gtagcgctag cggagtgtat actggcttaa tatgttacgg cggagtaaca
    10081 ctttataaaa gtgcttcatg tggcagggga aaatgcagct gcagggcgta cgggagaaca
    10141 tggaggttgc ggtgatatta cgcctcctcg ctcactcggt cgcttccgct cctgccgttc
    10201 ggctgcggcg ggcgttacct gcacacggat aagagcaaag gcatacggca tgacaaacag
    10261 ggcaagaaaa ggatgcggcg ctgccgtttt tccataggct ccgcccccct gacaagcatc
    10321 acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg
    10381 cgtt
//
