LOCUS       CP123604               12727 bp    DNA     circular BCT 26-APR-2023
DEFINITION  Enterobacter hormaechei strain RMCH-M19-N plasmid unnamed3, complete
            sequence.
ACCESSION   CP123604
VERSION     CP123604.1
DBLINK      BioProject: PRJNA956928
            BioSample: SAMN34233930
KEYWORDS    .
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 12727)
  AUTHORS   Workman,S.D., Islam,S., Akter,S., Haque,F., Nasrin,T., Mohanta,M.K.
            and Yost,C.K.
  TITLE     Complete Genome Sequences of Multidrug Resistant Bacteria Isolated
            from Cockroaches Collected in a Bangladeshi Hospital
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12727)
  AUTHORS   Workman,S.D., Mohanta,M.K. and Yost,C.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-APR-2023) Biology, University of Regina, 3737 Wascana
            Parkway, Regina, SK S4S 0A2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 118x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/20/2023 09:20:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,919
            CDSs (total)                      :: 4,801
            Genes (coding)                    :: 4,257
            CDSs (with protein)               :: 4,257
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 544
            CDSs (without protein)            :: 544
            Pseudo Genes (ambiguous residues) :: 0 of 544
            Pseudo Genes (frameshifted)       :: 451 of 544
            Pseudo Genes (incomplete)         :: 81 of 544
            Pseudo Genes (internal stop)      :: 35 of 544
            Pseudo Genes (multiple problems)  :: 21 of 544
            Pseudo Genes (short protein)      :: 1 of 544
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12727
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="RMCH-M19-N"
                     /isolation_source="Blattella germanica"
                     /db_xref="taxon:158836"
                     /plasmid="unnamed3"
                     /geo_loc_name="Bangladesh: Rajshahi"
                     /collection_date="2019-12-01/2020-03-17"
                     /collected_by="University of Rajshahi"
     gene            442..765
                     /locus_tag="QFB84_24490"
     CDS             442..765
                     /locus_tag="QFB84_24490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WGL89155.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMTIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            974..1408
                     /locus_tag="QFB84_24495"
                     /pseudo=""
     CDS             974..1408
                     /locus_tag="QFB84_24495"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1443..1934
                     /locus_tag="QFB84_24500"
     CDS             1443..1934
                     /locus_tag="QFB84_24500"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WGL89146.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1941..2156
                     /locus_tag="QFB84_24505"
     CDS             1941..2156
                     /locus_tag="QFB84_24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WGL89147.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2276..2698)
                     /gene="tolA"
                     /locus_tag="QFB84_24510"
     CDS             complement(2276..2698)
                     /gene="tolA"
                     /locus_tag="QFB84_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0098567 - periplasmic side of plasma membrane [Evidence
                     IEA]; GO_function: GO:0005215 - transporter activity
                     [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WGL89148.1"
                     /translation="MPLSVRFSPFMFFIPLFLTACTASPHTSVEKSAKAETTQPVDDLL
                     GDLKAGSSDNVASYATKIKHAVEEKMYKPESYKEQKCTIRLLLGRDGLVMGATAESGDS
                     ELCNAVMMAVKQANIPPAPDEKTWQEFRNTSLDFSY"
                     /name="tolA"
     gene            complement(3104..3562)
                     /locus_tag="QFB84_24515"
     CDS             complement(3104..3562)
                     /locus_tag="QFB84_24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WGL89149.1"
                     /translation="MAWHTVKDTLKLTGRSRSQLYRDMKKGLVSYRTGKDGRREFDTSE
                     LIRAYGELRTEETVKRHKKGQQEIDDTPAEHPQLKEIQQQLTALQQTVTLMLEDKSTRE
                     AELRQHDEERTQLLAEISRLNEDLEQEKKRSLWSRLFRRDIEEKNREK"
                     /name="entry exclusion protein 1"
     gene            3849..5174
                     /locus_tag="QFB84_24520"
     CDS             3849..5174
                     /locus_tag="QFB84_24520"
                     /inference="COORDINATES: protein motif:HMM:NF013211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0016020 - membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]; GO_process:
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WGL89150.1"
                     /translation="MPGFNYGGNSVGDGTNWSSERGSDPTTPGGGDNGHAGDHSSSGSS
                     SSTVEHTPWGDLTTDAAGYAYMNGIKLTADNSQLAYVSPTHLVRVLNSLLDRSYMKPEP
                     GEHSGVTGVPATSPSRVTLADNALKNAQSKAQDAAAALSKANAAISQAQTRLNTAKAKI
                     PSLEANVNTKQQASDKATARAHELAHFLNDMGGPGRYNALNAGYQSEVAQSELDNAKKA
                     LANGHSEITAAQKALDDAKKALPNATANKQKADSAVASAADMKDAITTTTAFFESISKK
                     FGERAARLAQELAAQARSAKIRSVPEAVALFDKHKGTAVNKLNAADKAAIANAFKSLDQ
                     AAMAKQLAAYSKVLGHFNKAMDFADLGAAVKKGIETGNWNDAINKLESIALTNTAGQLA
                     AIAFAAAGAPLGIVGFGIVMTLSGVLFSNEKVLSAINTSLGM"
                     /name="colicin-like pore-forming protein"
     gene            complement(5194..5547)
                     /locus_tag="QFB84_24525"
     CDS             complement(5194..5547)
                     /locus_tag="QFB84_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGL89151.1"
                     /translation="MTIKYYLKHCLWGLIGCGYLTYLILTDLNSGDIYPAYAPYMPYIA
                     SYLAVSAVIFPFAFYTTEQLSLKKMKRETWGNYFGSDSPSWGAFIFVYIFCVLLSVPLF
                     FIYPFINKNRPGN"
                     /name="hypothetical protein"
     gene            5712..5822
                     /locus_tag="QFB84_24530"
     CDS             5712..5822
                     /locus_tag="QFB84_24530"
                     /inference="COORDINATES: protein motif:HMM:NF014457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WGL89152.1"
                     /translation="MILAACQVNNVRDTGGGTVSPTSTVTGVTLDSGTNR"
                     /name="colicin release lysis protein"
     gene            6806..7129
                     /locus_tag="QFB84_24535"
     CDS             6806..7129
                     /locus_tag="QFB84_24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WGL89153.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMTIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7338..7772
                     /locus_tag="QFB84_24540"
                     /pseudo=""
     CDS             7338..7772
                     /locus_tag="QFB84_24540"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            7807..8298
                     /locus_tag="QFB84_24545"
     CDS             7807..8298
                     /locus_tag="QFB84_24545"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WGL89140.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            8305..8520
                     /locus_tag="QFB84_24550"
     CDS             8305..8520
                     /locus_tag="QFB84_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WGL89141.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     MLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(8640..9062)
                     /gene="tolA"
                     /locus_tag="QFB84_24555"
     CDS             complement(8640..9062)
                     /gene="tolA"
                     /locus_tag="QFB84_24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014063821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0098567 - periplasmic side of plasma membrane [Evidence
                     IEA]; GO_function: GO:0005215 - transporter activity
                     [Evidence IEA]; GO_process: GO:0006810 - transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WGL89142.1"
                     /translation="MPLSVRFSPFMFFIPLFLTACTASPHTSVEKSAKAETTQPVDDLL
                     GDLKAGSSDNVASYATKIKHAVEEKMYKPESYKEQKCTIRLLLGRDGLVMGATAESGDS
                     ELCNAVMMAVKQANIPPAPDEKTWQEFRNTSLDFSY"
                     /name="tolA"
     gene            complement(9468..9926)
                     /locus_tag="QFB84_24560"
     CDS             complement(9468..9926)
                     /locus_tag="QFB84_24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WGL89143.1"
                     /translation="MAWHTVKDTLKLTGRSRSQLYRDMKKGLVSYRTGKDGRREFDTSE
                     LIRAYGELRTEETVKRHKKGQQEIDDTPAEHPQLKEIQQQLTALQQTVTLMLEDKSTRE
                     AELRQHDEERTQLLAEISRLNEDLEQEKKRSLWSRLFRRDIEEKNREK"
                     /name="entry exclusion protein 1"
     gene            10213..11538
                     /locus_tag="QFB84_24565"
     CDS             10213..11538
                     /locus_tag="QFB84_24565"
                     /inference="COORDINATES: protein motif:HMM:NF013211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0016020 - membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]; GO_process:
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WGL89144.1"
                     /translation="MPGFNYGGNSVGDGTNWSSERGSDPTTPGGGDNGHAGDHSSSGSS
                     SSTVEHTPWGDLTTDAAGYAYMNGIKLTADNSQLAYVSPTHLVRVLNSLLDRSYMKPEP
                     GEHSGVTGVPATSPSRVTLADNALKNAQSKAQDAAAALSKANAAISQAQTRLNTAKAKI
                     PSLEANVNTKQQASDKATARAHELAHFLNDMGGPGRYNALNAGYQSEVAQSELDNAKKA
                     LANGHSEITAAQKALDDAKKALPNATANKQKADSAVASAADMKDAITTTTAFFESISKK
                     FGERAARLAQELAAQARSAKIRSVPEAVALFDKHKGTAVNKLNAADKAAIANAFKSLDQ
                     AAMAKQLAAYSKVLGHFNKAMDFADLGAAVKKGIETGNWNDAINKLESIALTNTAGQLA
                     AIAFAAAGAPLGIVGFGIVMTLSGVLFSNEKVLSAINTSLGM"
                     /name="colicin-like pore-forming protein"
     gene            complement(11558..11911)
                     /locus_tag="QFB84_24570"
     CDS             complement(11558..11911)
                     /locus_tag="QFB84_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WGL89145.1"
                     /translation="MTIKYYLKHCLWGLIGCGYLTYLILTDLNSGDIYPAYAPYMPYIA
                     SYLAVSAVIFPFAFYTTEQLSLKKMKRETWGNYFGSDSPSWGAFIFVYIFCVLLSVPLF
                     FIYPFINKNRPGN"
                     /name="hypothetical protein"
     gene            12075..12185
                     /locus_tag="QFB84_24575"
     CDS             12075..12185
                     /locus_tag="QFB84_24575"
                     /inference="COORDINATES: protein motif:HMM:NF014457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WGL89154.1"
                     /translation="MILAACQVNNVRDTGGGTVSPTSTVTGVTLDSGTNR"
                     /name="colicin release lysis protein"
     Typing          158..330
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.773"
                     /coverage="100.0"
                     /evalue="9.61e-56"
     Typing          6522..6694
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.773"
                     /coverage="100.0"
                     /evalue="9.61e-56"
     Typing          complement(2..191)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.241"
                     /coverage="99.0"
                     /evalue="2.46e-76"
     Typing          complement(6033..6555)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.847"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(12199..12704)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.046"
                     /coverage="99.0"
                     /evalue="1e-169"
     Typing          443..759
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="7.36e-61"
     Typing          756..2248
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.4"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6807..7123
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="7.36e-61"
     Typing          7120..8612
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.4"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcttgtca ggggggcgga gcctatggaa aaacggctcc gccgtggcct tctcgcttcc
       61 ctgttaagtt tcttcctggc atcttccagg aaatttccgc cccgttcgta agccatttcc
      121 gctcgccgca gccgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc
      181 ctgtatcaca tattctgctg acgcgctggt gccacctttt tctcctgcta catgaagcac
      241 tttcataaag tgttactccg ccgtaacata ttaagccagt atacactccg ctagcgctac
      301 gtgactggtt cagggctgcg ccccgaaacc cgccaaaagc ctgacgcgcc tgcggcttgt
      361 ccaaccccgg ccgaccggga aaggttttcc cgcccgtccc ggcgttatca ggagaccggt
      421 ttgtcagaga aacggaacaa gatgctcacc atgtgggtga cggaggacga gcaccggcgg
      481 ctgcttgagc gctgcgacgg caggcagctg gcagcgtgga tgcggcagac ctgcctggac
      541 gaaaagcctg cgcgtaccgg aaagcttccc tcgatctcgc cggcgctgct gcgccagctt
      601 gccggcatgg gcaacaacct gaaccagata gcccgccgcg ttaacgccgg cggtggcact
      661 ggccatgaca gggtgcagat tgtggcggcg ctgatgacca tcgatgccgg acttgagcgg
      721 ctgcggcacg ccgttcttga gaaaggaacg gacgatgatc gttaaatttc atccacgcgg
      781 tcgcggcggt ggcgccgggc cggtggacta cctgctgggt aaagaccggc agcgtgatgg
      841 tgccaccgtc ctgcagggga agccggaaga agtccgggag ctgattgacg cgtcgcctta
      901 cgtgaagaaa tacacttccg gcgtcctgtc gtttgcggag gccgatctgc ctcccggcca
      961 gcgggaaaaa ctgatggcca gcttcgagcg ggttctgatg cccggacttg ataaagatca
     1021 gtacagcatt ctgtgggtgg agcacgagga caaggggcgt ctggagctga actttctgat
     1081 ccccaatacc gagctgctga ccggcaggcg gctccagccg tactacgacc gcgccgaccg
     1141 gccgcgcatc gatgcctggc agaccatcgt gaacggcaga ctggggctgc acgacccgaa
     1201 cgcgccggaa aaccgccggg cgctggtcac gccgtccgga cttccgaaaa cgaagcagga
     1261 ggccgcagaa gccatcacgc gcggactgct gacccttgct tcgtccgggg agctgaaaag
     1321 ccgtcaggac gtcactgagg cgctggaacg gtcaggtttt gaggtggtgc gcaccacgaa
     1381 aagcagtatc agcattgccg acccggacgg ggggcgaaac atccgactga agggagccat
     1441 ctatgagcag tcttttaacg ctggcgaagg acttagagca gcaatcgaaa gcgcagaagc
     1501 agagtaccgg cgagatgctg aaagccgcat tcagcgagca cgagcagtct gtcagagcgg
     1561 aactgagcgc aagcgcgagg agaatcagcg acgccatcct cgcccacgag cagagtatga
     1621 gcgaggcgat ggagaaaaac cggcggagcg tactgcggac tgccgggcgg acatggctca
     1681 ccatcctgat ggtgtcagcg ctgctgatcg gcacgagcgg gagcattctg tggtggcagg
     1741 ggcagcagat aaccgacaat tacacgcatc ttcgtcagca ggaagatacc ctggcgaaaa
     1801 tgaccgcccg gacgtgggga gtgcgctatc aggagagcag cgacggccgg aggtttctta
     1861 tcctgccgcc ggggatgcaa accgaagcca tcccttacga cgggacgaca tggatccgcc
     1921 tgaaacagga gtagcggaaa atgacagagc tggaaaagca gttgctgagc gcattagagc
     1981 agctacagca ggactactcg caaaggctgg acgaatggga gagcgccttc gcggaatggc
     2041 ggagcatgtg tggtcttatg caacgggaga acgcgatgct gaacgagcgc gtgtcggact
     2101 tgagcacgca ggtgctgagt ttaagcgagc agctgcgccg cttgtccggg aactgaatgc
     2161 catcgagcgc cggcagagag ataatcagta tcagaaaaca ctagagctgg agcggcagcc
     2221 gcagaaaacc taccggggac cgacactgtg actgacctcc agcaggggaa atctttcagt
     2281 atgaaaaatc tagagaggta tttctgaatt cctgccaagt tttttcatcc ggtgcaggag
     2341 gaatattcgc ttgtttaaca gccatcatta ctgcgttaca aagctctgag tccccgctct
     2401 ccgctgttgc gcccataact aaaccatcgc gcccgagtaa gagccgaata gtacatttct
     2461 gctccttata tgattcaggt ttgtacatct tttcttcgac ggcgtgtttt atttttgttg
     2521 cataactggc gacattatcg cttgaacctg cttttaaatc ccctagcagg tcgtcaacgg
     2581 gctgggttgt ttctgctttt gccgattttt ctacgcttgt atgtggagag gcagtgcatg
     2641 ccgtcaaaaa cagcgggata aagaacataa atggtgaaaa gcgtactgat aaaggcatcg
     2701 cggcattcct tgttaacata aaggagccat cataattctg tggttaaacc aaagtaaact
     2761 atcagaaatc acgctctcgc catccgtttc ccgtcagatt aagaatcatg catctttctg
     2821 catagtcatg catgccgata aaacctggca ggtgcgtttc tggcggtttt ccgggtggtt
     2881 tgttgcctgt tttatcccgt agccgccgga aatgccctca ggccgtctga gcggtgcgcg
     2941 taatgagacg ttatggtaaa tcgtttgtca gcagctggtg acatagcacc agagcaagta
     3001 caacgccagc ataaaccaaa tcccccactt cagacaccat cgccagtatc tgaacagatg
     3061 cttctcccgt gtatccgctt catgaatgac gttctccaaa ctgtcatttt tcacgatttt
     3121 tctcctcaat atcacgtcgg aacaacctgg accagaggct gcgttttttt tcctgctcca
     3181 gatcttcatt cagtcggctg atttcggcca gcagctgtgt ccgctcctca tcgtgttgcc
     3241 ggagttcggc ttcgcgagtg cttttatcct ccaacatcaa tgtgaccgtc tgctgcaggg
     3301 cagttaactg ctgttgtatc tctttcagtt gcgggtgttc agctggagta tcgtcaattt
     3361 cttgctgtcc ctttttgtgc cttttgactg tctcctccgt ccgcagttca ccgtaagccc
     3421 gaatcagctc gctggtgtcg aattctcgcc gtccatcttt ccctgtgcgg taactgacaa
     3481 ggcctttttt catgtcccga tacagctggg atcgcgaacg accggtcagt tttagtgtgt
     3541 cctttacggt gtgccaagcc ataaaaatta ccgtgtctcc tgtatgtctc atgttgagct
     3601 cgcagattat atttctgttt taacatggca tatctaaggt tgtccgcagg aagtgaaaaa
     3661 accacaaaaa aatcatgttg acgatgtgct tttttcgggc gtagtattag atgtactgta
     3721 tataaacaca ttgttatata tacagtatta tggtgtggaa gagtacttat aacagcgcgc
     3781 tgcggaaacg gcaggcaaca aaccgcacac ctgatttcta ataaaaataa aacaaaaagg
     3841 gaaaaattat gcctgggttt aattatggtg gtaacagtgt aggggatggt actaattgga
     3901 gttctgagcg gggttctgac cccactacgc cagggggcgg agataatggt catgctggag
     3961 atcattcttc atctggtagt tcgtcatcaa ctgttgaaca taccccttgg ggagacctga
     4021 caaccgacgc tgccggctat gcttatatga atggtattaa actgacggca gataactcgc
     4081 aacttgctta cgtttctcca actcatcttg tgcgagtact taattctctg ctcgatcgtt
     4141 cttacatgaa acccgagcca ggtgaacatt ccggtgtgac gggggtgcct gcgacatccc
     4201 caagccgtgt aaccctcgca gataacgcgc tgaagaatgc acaatcaaaa gcccaagatg
     4261 ccgcagctgc tctgtcgaaa gcaaatgcgg ctattagtca ggcgcaaaca cgtctgaata
     4321 cagcaaaagc gaaaatccct agtcttgaag ccaatgttaa cacgaagcaa caggcttcag
     4381 ataaggccac tgctcgtgcg catgagctgg ctcatttcct gaatgatatg ggtgggcccg
     4441 ggcgttataa tgccttgaat gccggttatc agtctgaagt tgcacagagt gagctcgata
     4501 acgctaaaaa ggcgttagca aatggccatt ctgaaattac cgctgcacag aaagctttgg
     4561 atgatgctaa aaaggcttta cctaatgcca cagcaaacaa acagaaggct gacagtgctg
     4621 tggcatcagc tgctgatatg aaagatgcga ttaccactac tacagccttt ttcgagtcaa
     4681 tttcgaaaaa attcggtgaa cgggctgctc gccttgcgca ggaactggct gcccaggctc
     4741 gtagtgcaaa aatccgtagt gtgcctgagg cggttgcttt gtttgataaa cataagggta
     4801 ctgctgtaaa caaactgaac gcagcagata aggcggcgat cgcgaatgcg tttaaatctc
     4861 tcgatcaggc agccatggca aaacaattgg ccgcgtattc aaaggtttta gggcacttta
     4921 ataaggcgat ggattttgct gatttaggtg cggcggtaaa aaaaggtatt gagacaggta
     4981 attggaacga tgccatcaac aagcttgaat ctattgcttt aactaatacg gctggacagt
     5041 tagctgcgat tgcttttgcg gcagcaggtg cgccacttgg gattgtgggc tttggtattg
     5101 tcatgaccct gtctggtgtc ttatttagta acgaaaaagt tttgagtgca attaatacat
     5161 cgttaggcat gtagccagtt tccgtccggg gtgctagtta cccggacggt ttttattaat
     5221 gaaaggataa ataaaaaaca gtggaactga taacagtacg caaaaaatat aaacaaaaat
     5281 gaaagcaccc caagatgggc tgtctgaacc gaaataatta ccccatgttt ctcttttcat
     5341 tttttttaat gataattgtt ctgtggtgta aaaagcaaaa gggaagatga ctgcacttac
     5401 cgctaagtag ctagctatgt aaggcatata aggcgcataa gctggataaa tgtctccact
     5461 atttaaatct gtcaagatta agtaagtaag atatccgcag ccaatcaaac cccagagaca
     5521 atgttttaag taatatttaa tagtcatttt ttttgtttaa gttgaaatgc attcttgatt
     5581 ttatctaaaa aactgtcttt ggacaatgga tgaattgtaa gaccgaaagt tattataaat
     5641 gtagcctaaa ataataaaaa gaaggataga atatgcgaat atcttttttc tcgggaatcg
     5701 tcatagtatc gatgattctc gcagcatgcc aagtgaataa cgtccgagat actggaggtg
     5761 gtacagtttc tccaacatcc acagtaacgg gcgttacgct cgacagtggt acgaataggt
     5821 aacgatccat aaaggatctg cttgagatct tttgcgatga gtcgtaatct cttgctctgt
     5881 aaacgaaaaa accacccggc aaggtggttt ttcgaaggat cagaaaccgg caagtttctg
     5941 aaccaggtaa cagtcttgtg cgagacatgt caccaaatct gtcctttcag tgtagccgca
     6001 gtctggccat cacttcaagt actctcaata catctctcgc acaacctgct taccagtggc
     6061 tgctgccagt ggcgtttttt cgtgtctttc cgggttggac tcaagtcgat agttaccgga
     6121 acaggcgcag cagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     6181 tgccttcccg gaaccgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa
     6241 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcta ccagggggaa
     6301 acgcctggga tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattc
     6361 tgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ctccgccgtg gccttctcgc
     6421 ttccctgtta agtttcttcc tggcatcttc caggaaattt ccgccccgtt cgtaagccat
     6481 ttccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata
     6541 tatcctgtat cacatattct gctgacgcgc tggtgccacc tttttctcct gctacatgaa
     6601 gcactttcat aaagtgttac tccgccgtaa catattaagc cagtatacac tccgctagcg
     6661 ctacgtgact ggttcagggc tgcgccccga aacccgccaa aagcctgacg cgcctgcggc
     6721 ttgtccaacc ccggccgacc gggaaaggtt ttcccgcccg tcccggcgtt atcaggagac
     6781 cggtttgtca gagaaacgga acaagatgct caccatgtgg gtgacggagg acgagcaccg
     6841 gcggctgctt gagcgctgcg acggcaggca gctggcagcg tggatgcggc agacctgcct
     6901 ggacgaaaag cctgcgcgta ccggaaagct tccctcgatc tcgccggcgc tgctgcgcca
     6961 gcttgccggc atgggcaaca acctgaacca gatagcccgc cgcgttaacg ccggcggtgg
     7021 cactggccat gacagggtgc agattgtggc ggcgctgatg accatcgatg ccggacttga
     7081 gcggctgcgg cacgccgttc ttgagaaagg aacggacgat gatcgttaaa tttcatccac
     7141 gcggtcgcgg cggtggcgcc gggccggtgg actacctgct gggtaaagac cggcagcgtg
     7201 atggtgccac cgtcctgcag gggaagccgg aagaagtccg ggagctgatt gacgcgtcgc
     7261 cttacgtgaa gaaatacact tccggcgtcc tgtcgtttgc ggaggccgat ctgcctcccg
     7321 gccagcggga aaaactgatg gccagcttcg agcgggttct gatgcccgga cttgataaag
     7381 atcagtacag cattctgtgg gtggagcacg aggacaaggg gcgtctggag ctgaactttc
     7441 tgatccccaa taccgagctg ctgaccggca ggcggctcca gccgtactac gaccgcgccg
     7501 accggccgcg catcgatgcc tggcagacca tcgtgaacgg cagactgggg ctgcacgacc
     7561 cgaacgcgcc ggaaaaccgc cgggcgctgg tcacgccgtc cggacttccg aaaacgaagc
     7621 aggaggccgc agaagccatc acgcgcggac tgctgaccct tgcttcgtcc ggggagctga
     7681 aaagccgtca ggacgtcact gaggcgctgg aacggtcagg ttttgaggtg gtgcgcacca
     7741 cgaaaagcag tatcagcatt gccgacccgg acggggggcg aaacatccga ctgaagggag
     7801 ccatctatga gcagtctttt aacgctggcg aaggacttag agcagcaatc gaaagcgcag
     7861 aagcagagta ccggcgagat gctgaaagcc gcattcagcg agcacgagca gtctgtcaga
     7921 gcggaactga gcgcaagcgc gaggagaatc agcgacgcca tcctcgccca cgagcagagt
     7981 atgagcgagg cgatggagaa aaaccggcgg agcgtactgc ggactgccgg gcggacatgg
     8041 ctcaccatcc tgatggtgtc agcgctgctg atcggcacga gcgggagcat tctgtggtgg
     8101 caggggcagc agataaccga caattacacg catcttcgtc agcaggaaga taccctggcg
     8161 aaaatgaccg cccggacgtg gggagtgcgc tatcaggaga gcagcgacgg ccggaggttt
     8221 cttatcctgc cgccggggat gcaaaccgaa gccatccctt acgacgggac gacatggatc
     8281 cgcctgaaac aggagtagcg gaaaatgaca gagctggaaa agcagttgct gagcgcatta
     8341 gagcagctac agcaggacta ctcgcaaagg ctggacgaat gggagagcgc cttcgcggaa
     8401 tggcggagca tgtgtggtct tatgcaacgg gagaacgcga tgctgaacga gcgcgtgtcg
     8461 gacttgagca cgcaggtgct gagtttaagc gagcagctgc gccgcttgtc cgggaactga
     8521 atgccatcga gcgccggcag agagataatc agtatcagaa aacactagag ctggagcggc
     8581 agccgcagaa aacctaccgg ggaccgacac tgtgactgac ctccagcagg ggaaatcttt
     8641 cagtatgaaa aatctagaga ggtatttctg aattcctgcc aagttttttc atccggtgca
     8701 ggaggaatat tcgcttgttt aacagccatc attactgcgt tacaaagctc tgagtccccg
     8761 ctctccgctg ttgcgcccat aactaaacca tcgcgcccga gtaagagccg aatagtacat
     8821 ttctgctcct tatatgattc aggtttgtac atcttttctt cgacggcgtg ttttattttt
     8881 gttgcataac tggcgacatt atcgcttgaa cctgctttta aatcccctag caggtcgtca
     8941 acgggctggg ttgtttctgc ttttgccgat ttttctacgc ttgtatgtgg agaggcagtg
     9001 catgccgtca aaaacagcgg gataaagaac ataaatggtg aaaagcgtac tgataaaggc
     9061 atcgcggcat tccttgttaa cataaaggag ccatcataat tctgtggtta aaccaaagta
     9121 aactatcaga aatcacgctc tcgccatccg tttcccgtca gattaagaat catgcatctt
     9181 tctgcatagt catgcatgcc gataaaacct ggcaggtgcg tttctggcgg ttttccgggt
     9241 ggtttgttgc ctgttttatc ccgtagccgc cggaaatgcc ctcaggccgt ctgagcggtg
     9301 cgcgtaatga gacgttatgg taaatcgttt gtcagcagct ggtgacatag caccagagca
     9361 agtacaacgc cagcataaac caaatccccc acttcagaca ccatcgccag tatctgaaca
     9421 gatgcttctc ccgtgtatcc gcttcatgaa tgacgttctc caaactgtca tttttcacga
     9481 tttttctcct caatatcacg tcggaacaac ctggaccaga ggctgcgttt tttttcctgc
     9541 tccagatctt cattcagtcg gctgatttcg gccagcagct gtgtccgctc ctcatcgtgt
     9601 tgccggagtt cggcttcgcg agtgctttta tcctccaaca tcaatgtgac cgtctgctgc
     9661 agggcagtta actgctgttg tatctctttc agttgcgggt gttcagctgg agtatcgtca
     9721 atttcttgct gtcccttttt gtgccttttg actgtctcct ccgtccgcag ttcaccgtaa
     9781 gcccgaatca gctcgctggt gtcgaattct cgccgtccat ctttccctgt gcggtaactg
     9841 acaaggcctt ttttcatgtc ccgatacagc tgggatcgcg aacgaccggt cagttttagt
     9901 gtgtccttta cggtgtgcca agccataaaa attaccgtgt ctcctgtatg tctcatgttg
     9961 agctcgcaga ttatatttct gttttaacat ggcatatcta aggttgtccg caggaagtga
    10021 aaaaaccaca aaaaaatcat gttgacgatg tgcttttttc gggcgtagta ttagatgtac
    10081 tgtatataaa cacattgtta tatatacagt attatggtgt ggaagagtac ttataacagc
    10141 gcgctgcgga aacggcaggc aacaaaccgc acacctgatt tctaataaaa ataaaacaaa
    10201 aagggaaaaa ttatgcctgg gtttaattat ggtggtaaca gtgtagggga tggtactaat
    10261 tggagttctg agcggggttc tgaccccact acgccagggg gcggagataa tggtcatgct
    10321 ggagatcatt cttcatctgg tagttcgtca tcaactgttg aacatacccc ttggggagac
    10381 ctgacaaccg acgctgccgg ctatgcttat atgaatggta ttaaactgac ggcagataac
    10441 tcgcaacttg cttacgtttc tccaactcat cttgtgcgag tacttaattc tctgctcgat
    10501 cgttcttaca tgaaacccga gccaggtgaa cattccggtg tgacgggggt gcctgcgaca
    10561 tccccaagcc gtgtaaccct cgcagataac gcgctgaaga atgcacaatc aaaagcccaa
    10621 gatgccgcag ctgctctgtc gaaagcaaat gcggctatta gtcaggcgca aacacgtctg
    10681 aatacagcaa aagcgaaaat ccctagtctt gaagccaatg ttaacacgaa gcaacaggct
    10741 tcagataagg ccactgctcg tgcgcatgag ctggctcatt tcctgaatga tatgggtggg
    10801 cccgggcgtt ataatgcctt gaatgccggt tatcagtctg aagttgcaca gagtgagctc
    10861 gataacgcta aaaaggcgtt agcaaatggc cattctgaaa ttaccgctgc acagaaagct
    10921 ttggatgatg ctaaaaaggc tttacctaat gccacagcaa acaaacagaa ggctgacagt
    10981 gctgtggcat cagctgctga tatgaaagat gcgattacca ctactacagc ctttttcgag
    11041 tcaatttcga aaaaattcgg tgaacgggct gctcgccttg cgcaggaact ggctgcccag
    11101 gctcgtagtg caaaaatccg tagtgtgcct gaggcggttg ctttgtttga taaacataag
    11161 ggtactgctg taaacaaact gaacgcagca gataaggcgg cgatcgcgaa tgcgtttaaa
    11221 tctctcgatc aggcagccat ggcaaaacaa ttggccgcgt attcaaaggt tttagggcac
    11281 tttaataagg cgatggattt tgctgattta ggtgcggcgg taaaaaaagg tattgagaca
    11341 ggtaattgga acgatgccat caacaagctt gaatctattg ctttaactaa tacggctgga
    11401 cagttagctg cgattgcttt tgcggcagca ggtgcgccac ttgggattgt gggctttggt
    11461 attgtcatga ccctgtctgg tgtcttattt agtaacgaaa aagttttgag tgcaattaat
    11521 acatcgttag gcatgtagcc agtttccgtc cggggtgcta gttacccgga cggtttttat
    11581 taatgaaagg ataaataaaa aacagtggaa ctgataacag tacgcaaaaa atataaacaa
    11641 aaatgaaagc accccaagat gggctgtctg aaccgaaata attaccccat gtttctcttt
    11701 tcattttttt taatgataat tgttctgtgg tgtaaaaagc aaaagggaag atgactgcac
    11761 ttaccgctaa gtagctagct atgtaaggca tataaggcgc ataagctgga taaatgtctc
    11821 cactatttaa atctgtcaag attaagtaag taagatatcc gcagccaatc aaaccccaga
    11881 gacaatgttt taagtaatat ttaatagtca tttttttgtt taagttgaaa tgcattcttg
    11941 attttatcta aaaaactgtc tttggacaat ggatgaattg taagaccgaa agttattata
    12001 aatgtagcct aaaataataa aaagaaggat agaatatgcg aatatctttt ttctcgggaa
    12061 tcgtcatagt atcgatgatt ctcgcagcat gccaagtgaa taacgtccga gatactggag
    12121 gtggtacagt ttctccaaca tccacagtaa cgggcgttac gctcgacagt ggtacgaata
    12181 ggtaacgatc cataaaggat ctgcttgaga tcttttgcga tgagtcgtaa tctcttgctc
    12241 tgtaaacgaa aaaaccaccc ggcaaggtgg tttttcgaag gatcagaaac cggcaagttt
    12301 ctgaaccagg taacagtctt gtgcgagaca tgtcaccaaa tctgtccttt cagtgtagcc
    12361 gcagtctggc catcacttca agtactctca atacatctct cgcacaacct gcttaccagt
    12421 ggctgctgcc agtggcgttt tttcgtgtct ttccgggttg gactcaagtc gatagttacc
    12481 ggaacaggcg cagcagtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg
    12541 aactgccttc ccggaaccga gtgtcaggcg tggaatgaga taaacgcggc cataacagcg
    12601 gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ctaccagggg
    12661 gaaacgcctg ggatctttat agtcctgtcg ggtttcgcca ccactgattt gagcgtcaga
    12721 ttctgtg
//
