LOCUS       CP122856               12896 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC1723 plasmid unnamed4, complete
            sequence.
ACCESSION   CP122856
VERSION     CP122856.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549602
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12896)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12896)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 203x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 13:07:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,195
            CDSs (total)                      :: 6,061
            Genes (coding)                    :: 5,579
            CDSs (with protein)               :: 5,579
            Genes (RNA)                       :: 134
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 482
            CDSs (without protein)            :: 482
            Pseudo Genes (ambiguous residues) :: 0 of 482
            Pseudo Genes (frameshifted)       :: 321 of 482
            Pseudo Genes (incomplete)         :: 157 of 482
            Pseudo Genes (internal stop)      :: 67 of 482
            Pseudo Genes (multiple problems)  :: 61 of 482
            Pseudo Genes (short protein)      :: 5 of 482
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1723"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Belgium"
                     /collection_date="2020"
                     /collected_by="IMM_DI04"
     gene            1..120
                     /gene="tnpB"
                     /locus_tag="QDW93_30070"
                     /pseudo=""
     CDS             1..120
                     /gene="tnpB"
                     /locus_tag="QDW93_30070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /name="tnpB"
     gene            complement(289..1497)
                     /locus_tag="QDW93_30075"
     CDS             complement(289..1497)
                     /locus_tag="QDW93_30075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WHG29596.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLA
                     CGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVH
                     LVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSHITTE
                     SQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFR
                     YLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGM
                     DKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNARDISLLRY
                     MPA"
                     /name="IS91 family transposase"
     gene            complement(1478..1750)
                     /locus_tag="QDW93_30080"
     CDS             complement(1478..1750)
                     /locus_tag="QDW93_30080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG29597.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSCT
                     VCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
                     /name="hypothetical protein"
     gene            complement(1833..2708)
                     /locus_tag="QDW93_30085"
                     /pseudo=""
     CDS             complement(1833..2708)
                     /locus_tag="QDW93_30085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            2710..3507
                     /locus_tag="QDW93_30090"
     CDS             2710..3507
                     /locus_tag="QDW93_30090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011638903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0008484 - sulfuric ester hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="WHG29602.1"
                     /translation="MSLWDALRMNMMISYQELVRTFPNAQDTVIDVLSHSGIKVQWFDN
                     DSGCKGVCDQVENLTIDLKSDPKLCSGQYCFDQVLLNKLDKILAVAPSQDTVIFLHIIG
                     SHGPTYYLRYPPEHRKFIPDCPRSDIQNCSQEELINTYDNTILYTDFILSEVVNKLKGK
                     QDMFDTAMLYLSDHGESLGEKGMYLHGAPYSIAPKEQTSVPMLAWVSNDFSQDNQLNMT
                     CVAQRAEQGGFSHDNLFDSLLGLMNVKTTVYQSQLDIFAPCRY"
                     /name="sulfatase-like hydrolase/transferase"
     gene            complement(3845..4132)
                     /locus_tag="QDW93_30095"
     CDS             complement(3845..4132)
                     /locus_tag="QDW93_30095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WHG29598.1"
                     /translation="MSKVKGIAWEHVRNELMSNPEVQAAYEAEERKERLQAMLAEWRHH
                     AGLTRAQVAERMGVTPPTVSRMESNVIKASIETLARYARACGVKHPQITL"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4125..4442)
                     /locus_tag="QDW93_30100"
     CDS             complement(4125..4442)
                     /locus_tag="QDW93_30100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WHG29599.1"
                     /translation="MFNVILHDDVPNELAGLPPVVRAKMIRLIDKLKTNATALREPDSK
                     PLRDGLFELRTMGTDIARGLYVYQKGRNIYLLRVFIKKTQKTPSGEISMAFERLKEMLN
                     E"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4689..5102)
                     /locus_tag="QDW93_30105"
     CDS             complement(4689..5102)
                     /locus_tag="QDW93_30105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG29600.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            complement(5110..6060)
                     /locus_tag="QDW93_30110"
     CDS             complement(5110..6060)
                     /locus_tag="QDW93_30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003887 - DNA-directed DNA polymerase activity [Evidence
                     IEA]; GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WHG29601.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            complement(6653..6916)
                     /gene="traD"
                     /locus_tag="QDW93_30115"
                     /pseudo=""
     CDS             complement(6653..6916)
                     /gene="traD"
                     /locus_tag="QDW93_30115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /name="traD"
     gene            complement(7086..8294)
                     /locus_tag="QDW93_30120"
     CDS             complement(7086..8294)
                     /locus_tag="QDW93_30120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WHG29591.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLA
                     CGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVH
                     LVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSHITTE
                     SQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFR
                     YLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGM
                     DKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNARDISLLRY
                     MPA"
                     /name="IS91 family transposase"
     gene            complement(8275..8547)
                     /locus_tag="QDW93_30125"
     CDS             complement(8275..8547)
                     /locus_tag="QDW93_30125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG29592.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSCT
                     VCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
                     /name="hypothetical protein"
     gene            complement(9659..9850)
                     /locus_tag="QDW93_30130"
     CDS             complement(9659..9850)
                     /locus_tag="QDW93_30130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WHG29593.1"
                     /translation="MTKQEKTAINMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9915..10238
                     /locus_tag="QDW93_30135"
     CDS             9915..10238
                     /locus_tag="QDW93_30135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WHG29603.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            10447..10878
                     /locus_tag="QDW93_30140"
                     /pseudo=""
     CDS             10447..10878
                     /locus_tag="QDW93_30140"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            10916..11434
                     /locus_tag="QDW93_30145"
     CDS             10916..11434
                     /locus_tag="QDW93_30145"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WHG29594.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            11441..11674
                     /locus_tag="QDW93_30150"
     CDS             11441..11674
                     /locus_tag="QDW93_30150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WHG29595.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(11873..12013)
                     /locus_tag="QDW93_30155"
     CDS             complement(11873..12013)
                     /locus_tag="QDW93_30155"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG29604.1"
                     /translation="MATRADSKWIYHNPLLRAPLKRVQRDHGSRQVKMATNHPKNCRDR
                     A"
                     /name="hypothetical protein"
     ARG             2741..3507
                     /name="MCR-4.6"
                     /gene="MCR-4.6"
                     /gene_name="MCR phosphoethanolamine transferase"
                     /tool="rgi"
                     /drug_class="peptide antibiotic"
                     /identity="100.0"
                     /coverage="47.13"
     Typing          9496..9578
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.81"
                     /coverage="98.0"
                     /evalue="1.83e-34"
     Typing          complement(5111..5286)
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(8925..9485)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.316"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          10229..11778
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.555"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgatatctc tccctgccgg ttcgcgtatc tggctggttg ccggtatcac cgatatgcga
       61 aatggcttta acggcctggc ctcaaaagtt cagaacgtcc tgaaggatga cccgttcacg
      121 tataggaaat tgaaaaacca gacgataccg ctgtccacgc cttatgccac gtgagccttt
      181 ctgcttttcc ctccactccg gtggtttctc tctctgcatg gtcgattttt ataccgtaac
      241 aggctgtttt ttatacctgc accgattccc gggcacactt agcctgtgtc aggccggcat
      301 atacctcagc agactgatat cccgcgcgtt tttcttcagc cctgatacat tcagccccgc
      361 aatggcccgg cggtatacca tccggccgcc acacagcacg cattcgaacg ggtcacgact
      421 caggaactgt ttcaccattt gtgcatagca cactttcgcc actggttccg gcttatccat
      481 ccccagagca cggtacacct gcggcagctt ctctccacac acccggttgg caaggaaccc
      541 gaagtatctc accatcttaa aaaacttctc cgggatgtgc tgtttcagcc tcgcgaccag
      601 ctcccgctgt gtcagcgttt ccgtcgccgt ttctcccgtt ttgtggtcca ggtaacggaa
      661 gctcagactc gctcctccgt tgtaatgcgc cagccgggaa gccgctattg gtggcttctt
      721 cagataacga cccaggtagc gtgccgtatt ccgccctccg gctgtcttct tcgacatgta
      781 cacatgccag tattttccgc cggctttcag caccaggctt ctccactgtg attccgtcgt
      841 gatatgtgac aacgattccg gcatcgccag accttctgac cacgctttca gaagcagctg
      901 ccgcatattc cacatccacc gcgaacgcat cgcgtctttc aggaagctca gctttttcca
      961 gtgtccgtgt ttattcagac ctccgcaggt cactgacaca tgcacatgcg gatgccagtt
     1021 gagacgacgg ccatacgtat ggatggcgca gaagataccg ggttccagac cccgtttccg
     1081 ggctgcatac agcagattct ccaccgccag acggcacacg tcattcagca gccagcggtt
     1141 gctttcgaac accggccaca gcgtgtccgg cagggtgaag accaggtgca cccagtcgca
     1201 gtcaggaaga cgattcagct gtgtcgctat ccacaggtct gtggcctttt ttccgcagga
     1261 cgggcaggca cggctgccac atgagttagt gacgtatctg acgtgggggc actcaggttt
     1321 atcgcagttg tattccttca cacccagtat ccgcgtgccg caggccagca tttttgtgac
     1381 agcctcaacc tcgatatcgc gcaggccgcc cgcatccagg aaggatgtcc agcactggtt
     1441 ggctgtgaac aggcgtttca gcggtcgggg ctgaaaatca ccaccacttc gggtcatccg
     1501 ccttcatctc cgcttcagtt tcgtataaat caaaacgacg gcaggtatgg cagaacgtga
     1561 cgtattcgta cggtgaatcc gggtacattt tgtggaaggt ttcccagaaa cagaccgtac
     1621 aggagggatg accggcgata aagtctgtaa atacagactg ataagggtga ttattggccc
     1681 tggcgacggc tttcagaacc tctttcacca ttctggtgtg gactttctgg tgctccaggt
     1741 tgtgtgacat gggaactcat tctggatggt tactctgaaa gctcatattc tgccacacca
     1801 cgatttgcag ccgccaggct gccgtggttc tatctggctt tcacgaagcc gaactgccgc
     1861 ttgatgatgc gaaacgggtg ctccaccctg gcacggatgc tggctttcat gtattcgatg
     1921 ttgatggccg ttttgttctt gcgcggattc tgcttcaagg tttttacctt gccgggacgc
     1981 tcggcgatca gccagtccac atccacctcg gccagctcct cgcgctgtgg cgctccttgg
     2041 tagccggcat cggctgagac aaattgctcc tctccatgaa gcagattacc cagctgattg
     2101 aggtcatgct cgttggccgc ggtggtgacc aggctgtggg tcaggccact cttggcatcg
     2161 acaccaatgt gggccttcat gccaaagtgc cactgattgc ctttcttggt ctgatgcatc
     2221 tccggatcgc gttgctgctc tttgttcttg gtagagctgg gtgcctcaat gatggtggca
     2281 tccaccaaag tgccttgggt catcatgacg cctgcttcgg ccagccagcg attgatggtc
     2341 ttgaacaatt gacgggccag ttgatgctgc tcgagcaggt ggcggaaatt catgatagtg
     2401 gtgcgatccg gcagggcgct atccagggat aatcgggcaa acaggcgcat ggaggcgatt
     2461 tcgtacaggg catcttccat ggcaccgtcg ctcaggttgt accaatgctg catgcagtga
     2521 atacgcagca tggtctccag cggatagggc cgtcggccat tgcccgcctt gggataaaac
     2581 ggctcgatga cagcggtcat attctgccat ggcagaatct gctccatgcg ggagaggaaa
     2641 atctcttttc gggtctgacg gcgcttagtg ctgaattcac tatcggcgaa ggtgagttga
     2701 tggctcatga tgtccctttg ggatgcgctc cggatgaata tgatgatctc atatcaggaa
     2761 cttgttcgca ccttccctaa tgctcaagac acagtgattg atgtgttaag tcatagtggt
     2821 ataaaagtac agtggtttga taatgattct ggctgtaaag gtgtgtgtga tcaggttgaa
     2881 aatctcacga tagatttgaa gagtgatccg aagctgtgtt ctggccaata ttgttttgac
     2941 caagtattgc tcaacaaatt agataaaatt ctggcagtag caccaagtca agatacagta
     3001 atttttttgc atatcattgg tagtcatgga ccaacttatt atcttagata cccgccagag
     3061 catcgtaaat ttataccgga ttgtccgcgc agtgatattc aaaattgcag tcaagaagaa
     3121 ctgattaaca cctacgacaa cactattcta tatacggatt ttattctcag tgaagtggtg
     3181 aataaattaa aaggtaagca ggatatgttc gatactgcaa tgctgtatct ctctgaccat
     3241 ggtgagtctt tgggtgaaaa gggcatgtat ttacatggtg cgccctatag tattgcaccg
     3301 aaagaacaaa ctagcgtacc aatgctggct tgggtatcta atgactttag ccaagataat
     3361 cagttgaaca tgacttgtgt tgcacagcga gcagaacagg gcggcttttc ccacgacaat
     3421 ttgttcgaca gtttgctagg acttatgaat gtaaaaacca ccgtctatca gagccaactc
     3481 gatatttttg caccttgcag gtattagata tggctgtaaa tgttttatct taggggtgac
     3541 atattcaccc cttaaatctt ggactaacaa tgtcctctat tgttacattt agctctcaat
     3601 cttccacaat gagttatcac ggtcttttag acttaacatc aatttgccgt tgtgaaacta
     3661 gaacaatggc ttacaagctg taaatgctaa ctttacattt ttggcgcgtt gggcaaaatt
     3721 agcctattta tatcccttag gattatctca tatgtgtgaa atgatggttt tttgcaaaaa
     3781 aactccttgt tcaacataag cgatcttgca cgcatcaaaa ggctgcttaa agcggccttg
     3841 aaaattacaa cgtgatctga gggtgcttaa caccgcatgc tcttgcgtat cgagccagtg
     3901 tttcaatgct agccttaatc acattagatt ccatgcgaga aacggtaggt ggagtaacac
     3961 ccatgcgttc tgccacctgg gctcttgtta atcctgcgtg atgacgccat tcagcgagca
     4021 tagcttgaag tctttccttg cgctcctctg cttcataggc tgcttgaacc tcaggattag
     4081 acatcaattc attcctaaca tgctcccaag cgataccttt aactttactc atttagcatc
     4141 tccttaagac gttcaaaggc catagatatt tcacccgatg gggttttctg agtcttttta
     4201 ataaagactc taagtagata gatattcctg cctttctgat aaacataaag acctctggca
     4261 atatccgtcc ccatcgtcct taactcaaat aatccatccc ggagtggttt gctatctggc
     4321 tctcgtaatg cagtagcgtt ggttttaagt ttatcaatca ggcgaatcat cttcgctctg
     4381 actactggcg gtaagccagc cagctcattt ggtacatcat cgtgcaatat gacgttaaac
     4441 actggttcgc cctccattgc gaaatattag cataaaaagc aatttgcgtt atagggtaat
     4501 tttatggttt ttctgctgta tttaacataa gcgatcttgc gcgcaccgcc cgaacaccac
     4561 tcgccacaaa aacccgccgg aacgtccaaa agtacgggtt ttgctgcccg caaacgggct
     4621 gttctggtgt tgctagtttg ttatcagaat cgcagatccg gcttcagccg gtttgccggc
     4681 tgaaagcgct atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc
     4741 tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg
     4801 tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt
     4861 gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg
     4921 atctgttcat ggtgaacagc tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct
     4981 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatgtaacgg
     5041 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
     5101 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
     5161 ttgcgtgagc catgagaacg aaccattgag atcatactta ctttgcatgt cactcaaaaa
     5221 ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct
     5281 tagtccgtta tgtaggtggg aatctgatgt aatggttgtt ggtattttgt caccattcat
     5341 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
     5401 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
     5461 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
     5521 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
     5581 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     5641 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     5701 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     5761 atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt
     5821 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     5881 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     5941 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     6001 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     6061 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     6121 ataattacta gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc
     6181 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     6241 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     6301 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattaaaa
     6361 aggatgtcgc aaacgctgtt tgctcctcta caaaacagac cttaaaaccc taaaggctta
     6421 agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc cttttgtctc cgaccatcag
     6481 gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt
     6541 gaatgggggg aaatggcact acaggcgcct tttatggatt catgcaagga aactacccat
     6601 aatacaagaa aagcccgtca cgggcttctc agggcgtttt atggcgggtc tgctatgtgg
     6661 tgctatctga ctttttgctg ttcagcagtt cctgccctct gattttccag tctgaccact
     6721 tcggattatc ccgtgacagg tcattcagac tggctaatgc acccagtaag gcagcggtat
     6781 catcaacagg cttacccgtc ttactgtcaa ccagacccgc caggataagc aatccggcag
     6841 actggtacag agcatggtca cgggctttac gggcggctct ggcttcggct cgcttttctg
     6901 cctgtatcag gttcacgtat aggaaattga aaaaccagac gataccgctg tccacgcctt
     6961 atgccacgtg agcctttctg cttttccctc cactccggtg gtttctctct ctgcatggtc
     7021 gatttttata ccgtaacagg ctgtttttta tacctgcacc gattcccggg cacacttagc
     7081 ctgtgtcagg ccggcatata cctcagcaga ctgatatccc gcgcgttttt cttcagccct
     7141 gatacattca gccccgcaat ggcccggcgg tataccatcc ggccgccaca cagcacgcat
     7201 tcgaacgggt cacgactcag gaactgtttc accatttgtg catagcacac tttcgccact
     7261 ggttccggct tatccatccc cagagcacgg tacacctgcg gcagcttctc tccacacacc
     7321 cggttggcaa ggaacccgaa gtatctcacc atcttaaaaa acttctccgg gatgtgctgt
     7381 ttcagcctcg cgaccagctc ccgctgtgtc agcgtttccg tcgccgtttc tcccgttttg
     7441 tggtccaggt aacggaagct cagactcgct cctccgttgt aatgcgccag ccgggaagcc
     7501 gctattggtg gcttcttcag ataacgaccc aggtagcgtg ccgtattccg ccctccggct
     7561 gtcttcttcg acatgtacac atgccagtat tttccgccgg ctttcagcac caggcttctc
     7621 cactgtgatt ccgtcgtgat atgtgacaac gattccggca tcgccagacc ttctgaccac
     7681 gctttcagaa gcagctgccg catattccac atccaccgcg aacgcatcgc gtctttcagg
     7741 aagctcagct ttttccagtg tccgtgttta ttcagacctc cgcaggtcac tgacacatgc
     7801 acatgcggat gccagttgag acgacggcca tacgtatgga tggcgcagaa gataccgggt
     7861 tccagacccc gtttccgggc tgcatacagc agattctcca ccgccagacg gcacacgtca
     7921 ttcagcagcc agcggttgct ttcgaacacc ggccacagcg tgtccggcag ggtgaagacc
     7981 aggtgcaccc agtcgcagtc aggaagacga ttcagctgtg tcgctatcca caggtctgtg
     8041 gccttttttc cgcaggacgg gcaggcacgg ctgccacatg agttagtgac gtatctgacg
     8101 tgggggcact caggtttatc gcagttgtat tccttcacac ccagtatccg cgtgccgcag
     8161 gccagcattt ttgtgacagc ctcaacctcg atatcgcgca ggccgcccgc atccaggaag
     8221 gatgtccagc actggttggc tgtgaacagg cgtttcagcg gtcggggctg aaaatcacca
     8281 ccacttcggg tcatccgcct tcatctccgc ttcagtttcg tataaatcaa aacgacggca
     8341 ggtatggcag aacgtgacgt attcgtacgg tgaatccggg tacattttgt ggaaggtttc
     8401 ccagaaacag accgtacagg agggatgacc ggcgataaag tctgtaaata cagactgata
     8461 agggtgatta ttggccctgg cgacggcttt cagaacctct ttcaccattc tggtgtggac
     8521 tttctggtgc tccaggttgt gtgacatggg aactcattct ggatggttac tctgaaagct
     8581 catattctgc cacaccacga tttgcagccg ccaggctgcc gtggttcgaa ctccatgatt
     8641 attccttggt aaaactatcc aaatagacct tgagttacac ccctatgatg ggtaatctct
     8701 acgggcgtaa acgaaaaaac ccacgttctc aggcgtgggt ttttcgaagg ttagaagttt
     8761 ctcaggcttt ctaacccaag gtaacagggc gtacaagacc gctgccacca aatctgtcct
     8821 ttcagtgtag ccgcagtcgg gccttcactt caagaactcc gtatcatgca atttcttgta
     8881 caccctctac caatggctgc tgacagtggc gttaagtcgt gtcttaccgg gttggactca
     8941 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9001 cccagcttgg agcgaacggc ctacaccgaa ccgagatacc aacagcgtga gctatgagaa
     9061 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9121 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9181 gggtttcgcc acctctgact tgagcgtcga tttctgtgat gctcgtcagg ggggcggagc
     9241 ctatggaaaa acgccagcgg cgtggccttc ttccgggttc ttgttttttg ctcacatgtt
     9301 tcctttccgg cgttatcccc tgattctgtg gataaccgca ttaccgtctt tgagtgagct
     9361 gataccgctc gccgcagccg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa
     9421 gagcgcctga tgcggtattt tctccttacg catctgtgcg gcatttcaca cccggcatgg
     9481 ggcacttttc atacaatctg cactgatgcc gcatagttaa gccagtatac actccgctag
     9541 cgctacgtga ctggttcagg gcttcgcccc gaaacccctc tgacgcgccc tgacgggctt
     9601 gtctgctccc ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgtatgtgtc
     9661 agaggttttc accgtcatcc ccgaagcgtg cgaggcagct gcggtaaagc tcgtcggcgt
     9721 ggtcgtgaag tgattcacaa atatcggcct gttcatctgc gtccagttcg ttgagtttct
     9781 ccagcagcgt taatgtctgg cttctgataa agcgggccat gttaatggcg gttttttcct
     9841 gtttagtcac tgatgcctcc gtgtaagggg gatttctgtt ttatggggta atgataccga
     9901 tgaaacgcga gaggatgctc acaatacggg ttactgatga tgaacatgcc cggttacttg
     9961 aacgctgtga gggtaaacag ctggcggtat ggatgcggcg ggtctgtctg ggtgagccgg
    10021 ttgcccgttc cgggaagctg ccgacactgg caccgccgtt actgcgccag ctggctgcca
    10081 tcgggaataa tctgaatcag acggcgagga aggtgaacag cgggcagtgg tcttccggtg
    10141 accgggttca ggtggtggcc gcactgatgg ccatcgagag agagttgcgc agcttacggc
    10201 aggtcgtcag ggaacatgga gcgcgggatg atagttaagt ttcatgccag gggaaaaggc
    10261 ggtggcagtg gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg
    10321 gtgcttcagg gtaatccgga agaagtccgg gaactcatcg atgccacgcc atttgcgaag
    10381 aaatacacgt caggtgttct gtcgttcgcg gagaaggagc tgccgccggg aggacgtgaa
    10441 aaggtgatgg cgagctttga gcgtgtactg atgcccggtc tcgaaaaaaa tcagtacagc
    10501 atcctgtggg tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac
    10561 atggagctac agagcggaaa acgcctccag ccgtactacg accgggcaga caggccgaga
    10621 attgatgcct ggcagacgct ggttaatcac cattacgggc tgcatgaccc caacgccccg
    10681 gagaaccgcc ggatactgac tctccctgat aacctgccgg aaacgaaaca ggcgcttgca
    10741 gagagcgtca cacgcgggat agacgctctt tatcatgtcg gggagataaa aggccgtcag
    10801 gatgtgattc aggcgctcac agaggcaggg cttgaagtgg tcagggtgac gcgaagcagt
    10861 atcagcattg cagacccgaa cggcgggaag aatatcaggc tgaaaggagc attttatgag
    10921 caatctttta cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac
    10981 agagacaatg ctgaacgacg agttcagcaa gctcgaaaaa tctgtaagca aggctgtgac
    11041 atcaaacgag acgaaaatca aagacgctat agccctgttc acagcttcga cagaggaatc
    11101 actgaaaaaa caccgggaag gggtgaaaga ggcgatgatg cagcacagga aggacgtgtt
    11161 aaagctggca gggaatacgg gcatgatgtt actggggatg gtcattttcc tgtttaccgt
    11221 gagtggcggg acgctctggt atcttggcgg gcggatacag acgaacctgg aagaaatcag
    11281 gaagcaggaa gagacattgc agaaactgaa cgcgaagaca tggggcgtgg agtttgtgca
    11341 ggacgggaga aggaaatttc tggtcatccc gcaggggaaa tcggcaacgg tgattcccta
    11401 tcaggggaag gactgggtac aactgacgga gtgacacaga gtgacggagc tggaaagaca
    11461 tttgctgaac gcattagagc aactgcaaca ggactatatg cagcggctga acgaatggga
    11521 gagcgccttc gtggaattgc agaaaatgtt ttcgcttacg caacgggaca acgcgatgct
    11581 gaacgagcag gtcatgcagt tgagtcagca ggtgcagcac ttgagcgagc ggacagaacg
    11641 cttgagccag ttatacagcg agaacaggag ataagggacg aacggctgat acaggaacgc
    11701 gaacatgagt tatccctgga gcgggaacgc cagccggaaa tacaggaacg cacactggat
    11761 ggtccttcac tgggatggtg aaactatgaa aaatggcagg ttaagtggat taagacgggc
    11821 taatgtggtc tgtaccatct ggttgcatag gtattcatgc ggttaaaatt tatcaggcgc
    11881 gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc
    11941 gctgaacgcg ttttagcggt gcgcgtaata agggattatg gtaaatccac ttactgtctg
    12001 ccctcgtagc catcagataa accgcagaaa tcgtgtcagc cacagcctga tttttctaag
    12061 gtctttgctg cggtaatcag taccccaaat tagttgtgcc ccggtagttt aaaattcctg
    12121 gctgtaaact gctaatgctt gttcacatca ctcctatttt cctgtcgctt tatctttagc
    12181 tcccaatccc aaactgccaa aaaacaccat cattcgtgtt gtgtcggctt ttgttaaccg
    12241 tctcatttaa accgtctttc gcctcctttc ctgtttccga tactaattcc attttcgcag
    12301 taaaacattt aaataatcac aaacgtactc aacaatccaa ttatcctgcg tgtgctacag
    12361 ctaatttgac gagtcactgt cgtacccgcg catttctgcc aaccgtgtca caaaaccgcc
    12421 atttttctct ggtaggcgat ttcacgtatt cactgactgg cctctttccg taatattcct
    12481 ccaactggcc cgctcttttc ccgcgatacc cttcagcccc gaccgtgact cagcctgtaa
    12541 acagaaaacc ccgccaaccg attttcaccg agtttaaata gactggttca gcaaactctt
    12601 tacccggtgc ttgtgtccac aaaagctcaa gaatgaatta acgataactg ctctttaact
    12661 ggcgcatctc tggcgtaacg gtggcctgca accgcccggc gaacataaac atcgctactt
    12721 ccctacgtta actccgagcc gataaaaatc ccaccagtca gatacctgaa cagataaata
    12781 cactccaaca gtctgtgctg cagctggttc tgccggccga actctcaggc tgaaggtgaa
    12841 ctgacaccgg cgttattaca gacacttatc cgcgaaatga aagggagcag ccactg
//
