LOCUS       CP122841                6229 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC1729 plasmid unnamed4, complete
            sequence.
ACCESSION   CP122841
VERSION     CP122841.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549606
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 237x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 13:06:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,450
            CDSs (total)                      :: 5,333
            Genes (coding)                    :: 4,902
            CDSs (with protein)               :: 4,902
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 431
            CDSs (without protein)            :: 431
            Pseudo Genes (ambiguous residues) :: 0 of 431
            Pseudo Genes (frameshifted)       :: 269 of 431
            Pseudo Genes (incomplete)         :: 170 of 431
            Pseudo Genes (internal stop)      :: 60 of 431
            Pseudo Genes (multiple problems)  :: 60 of 431
            Pseudo Genes (short protein)      :: 3 of 431
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1729"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Belgium"
                     /collection_date="2020"
                     /collected_by="IMM_DI04"
     gene            1..1017
                     /locus_tag="QDW97_25990"
     CDS             1..1017
                     /locus_tag="QDW97_25990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WHG34437.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIK"
                     /name="replication initiation protein"
     gene            complement(1281..1679)
                     /locus_tag="QDW97_25995"
     CDS             complement(1281..1679)
                     /locus_tag="QDW97_25995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG34438.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQAVQNRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1856..2782
                     /locus_tag="QDW97_26000"
     CDS             1856..2782
                     /locus_tag="QDW97_26000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WHG34439.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFETAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREYGFD
                     MRDGVSRQERTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2785..3318
                     /locus_tag="QDW97_26005"
     CDS             2785..3318
                     /locus_tag="QDW97_26005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG34440.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            3539..5266
                     /locus_tag="QDW97_26010"
     CDS             3539..5266
                     /locus_tag="QDW97_26010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WHG34434.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDSELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5269..5529
                     /locus_tag="QDW97_26015"
     CDS             5269..5529
                     /locus_tag="QDW97_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WHG34435.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     gene            5591..5734
                     /locus_tag="QDW97_26020"
     CDS             5591..5734
                     /locus_tag="QDW97_26020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WHG34436.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6229
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2..776
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1857..2779
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcgccg cgcttcaata cttcgacgaa aatttacccc atcgtcccta tcacacggat
       61 gatctcgctt tcggccttcg catctccggc aaagggcgtg cgcttcttgc gcggtacatc
      121 cagcagaacc agcctcatgc gcagttctgg ctggtttttg acgttgaccg cgagggggct
      181 gcgattgact ggagcgaccg gaacgccccc gcgccgaaca tcaccgttaa aaatcctgtg
      241 aacggacacg ctcacctgct ctacgcactc aacatcgccg tgagaaccgc gcctgatgcc
      301 tcggttaagg cgctgaaata tgccgcagcg gttgaacgtt cgctgtgtga gaaactttgt
      361 gctgatgtga actacagcgg cctgatttgc aaaaatccgt tccacctgga atggcaggtg
      421 atggagtggc gcgaggacgc ctataccctc gatgaactgg cagattatct cgatttgagc
      481 gcctcagagc gtcgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc
      541 gaaatgacgc gcaaatgggc ttacagggcg attcgtcagg gctggccagc gttctcacag
      601 tggcttgaag cggtgatcca gcgtgtcgaa atgtacaatg catcgcttcc cgttccgctt
      661 tcactggctg aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc
      721 acgccggaaa ctttcgcaca gtatgtggct gatacgcaca cgccagaaat tcaggcagca
      781 cgcggtcgca agggcgggaa ggccaattcg tcagaaaatc agtcggacaa agggaaaaaa
      841 agcgccgctg ttcggtggac tgctaacgac gacaaacgtc gtagggcgct tgatatgtat
      901 atcttgggag cgtcaacgga agatattgca gtggcggttg gtgttagcag ccggactatt
      961 cgacgctgga tggataattc tggagaatgg ctaaccaaga aacaaataat taaatgatag
     1021 ttaacaaaga agcaacacta ccccccgttt tttgttaatg ggatgttaaa ttcgcggaca
     1081 tgaacaagcc tatatcagat aacagcgcct ttcggcgtct ttttgagcag cgggctttcc
     1141 ggcggttttc tcctctcagc ccagcaatgg tgcggcttac cgcatgattg aggtggtcgt
     1201 gctcgccgca gtctcatgac cgagcgtagc gagcgaatga gcgaggaagc accagggaac
     1261 cggaacacgg atggaggcac ttacgcgtct gggtttgggg atgtctccag atcgtactta
     1321 atttccttaa gtgcctgtaa tatcctgtct tttttatagc atagctcgcc tgtttttgtg
     1381 tctaccagtc ctgcttcgat cagtagccca gccgcgagaa agagtcggtg attgcgttct
     1441 cgtcgttgtt tttttccctc gattcgattt tggaccgcct gttttcgttc gttctcctgt
     1501 gcctgtaagt cgtttttttc cgctgttgcc agtacgtgga ggagtttgcg ttcctgttct
     1561 gtgagtcctg cttcgttgtc gagcaggtca ataatctgtc tctgtcgtgc ggtctgttct
     1621 ccgagcttcc tgatgaatcc gatccgtttc ttcagccatt cgatgttgct gatgcgcatg
     1681 ttcgtggtgc tccctggtgt ttttttgcgg tgtgttgtgg tcgtgcagta tatcgcttgc
     1741 ggtcggcggg tgcagggggt atcttccgtg ctaattcgta aaattagaag gaagcgcact
     1801 tatatgtttc tctccttcgt cgagaaacag tgcgctcctg cggagggctg aaaccgtggc
     1861 aacctatcac ctcagcgtta aatttggtgg caaggggcag gctgcaaacc acgccgatta
     1921 catcgagcgc aaagagaaat accgcgaccg tcaggatttg gaatattccg cacacggaaa
     1981 catgccggaa tgggcgcgag ataatccctc acatttctgg caggcggctg accagtttga
     2041 acgtgcaaac ggttcgactt atcgggaact ggagatagcg ctgccccgtg aactgacacc
     2101 ggaacagcgc cttgaactgg tgcaggattt tgtacgtcag gaagctggcg aacggcatgc
     2161 atggagtttt gccattcaca atccgaaagc gagcattgag ggaggcgaac agcctcatgc
     2221 gcatatcatg atgagtcagc gtgtgaatga tggcattgaa cgtacaccgg aacagtattt
     2281 ccggcgctat aacgccaggt atcccgagcg gggaggtgcg aaaaaggatt caggaagcct
     2341 gactcccact cagcaaaaag agcagttgcg ggaattgcga aaacgctggg aggtgaagca
     2401 caacgaacat atgagaaagc atggctttga aaggggcttc attgactgcc ggacgctgaa
     2461 agaacagggc attgaacgac gtcctgaagt gcatctgggt tttgaaactg ccggacggct
     2521 tgatgatgcc cagcgtcatg acatcatgca aaaacggtct gaaccggact attcccgcca
     2581 tcttgaacag gcgcgaacgg tcgggcatga catcagggaa tggctggaca ctccggttaa
     2641 gcaacatgaa ctggtcagaa ggctgcaaca tgatgttgcg cgggagtatg gctttgacat
     2701 gagagacgga gtgagcagac aggagcgaac tgaacctgaa cgggttaaat caccgtcacg
     2761 tggattttct atgggattct gaacatggcc agcacagaag aaaaattact cacgctcctg
     2821 ttatcggtgg agcatctgca acataccgca atggagcaac agaaagctgt tgctgattct
     2881 ctggctgtgg caagggaaac acttcgcgga tatcgggata tttctgacgg tgtggcaaaa
     2941 caggtcaggg atgaagtcag aaatgaggtg cagaagctgg acgtggcagg actgataggt
     3001 gagcgcatca gccaggattt tcagcggctg gaagagtcag tcgcaaacat gaaggcaaac
     3061 gcggatgccc tgaacaggga tattgcgaca attcacagca atatcgtgca ggaatacaac
     3121 acccttaaag ggtatagctg gaaatggctg ggtggggtgt cagtggcttt tctggttatg
     3181 ttctgtgtgc tcggatgggg aatgaaaaca atactcgaca ggaactacaa cgccacagcg
     3241 gcggtgtatc agaaagttgt gacactggaa gataaactgg tggagaaacc tgtcacgggt
     3301 aagagccgga aaaactgacg cgcttcgctt gtccaacgcc tcacgacccg cgaaaggatt
     3361 tcgcgttccg ttcaccgttt tcatgagtga ttcctccgaa ctgccaaaaa atatcccccc
     3421 ctgacttgac agaaaaaata atggcgagta ctttttgatc tgtacataaa accagtggtt
     3481 ttatgtacag tattgactat gtaatcaatt gttttaacgc ttaaaagagg gaatttttat
     3541 gagcggtgga gatggacgcg accacaacag tggcgcacat aacacaggtg gtaacattaa
     3601 tggcggccct acggggcttg gtggaaatgg tggggcttct gacggttcag gatggagttc
     3661 ggaaaataat ccatggggtg gcggttccgg tagtggtgtt cactggggag gtggctccgg
     3721 caatggtaat ggcgggggga atggtaattc cggtggtggc agcaattcat ccgtagcagc
     3781 agtggcattt ggttttcctg ctttggcaac tcctggtgcc ggaacgctgg gtatcgccgt
     3841 ctctggtgag gctttatctg cggcaatagc tgatatcttt gcagccctga aaggtccgtt
     3901 taaattcagc gcatggggta ttgcgcttta cggcattctg ccatctgaaa tagcaaaaga
     3961 tgacccgaat atgatgtcaa agattgtgac gtcattaccg gcagaaacag tgacgaatgt
     4021 tcaggtcagt acacttccgc tggaccaggc aactgtcagc gtgacgaaac gtgtgacaga
     4081 tgttgtgaag gacacacgac agcatattgc cgtagttgca ggtgtgccta tgagtgtgcc
     4141 tgttgtgaat gccaaaccaa cacgtactcc aggtgtattt cacgcatcat ttcctggtgt
     4201 cccttctctg acagtcagca ctgtcaaagg ccttccggtg tcaacaaccc ttccccgtgg
     4261 tattacggag gataaaggcc ggactgccgt tcctgcagga tttacctttg gtggtggttc
     4321 acatgaagcg gtgatccgtt ttccgaaaga aagtgggcag aagccgattt atgtatcagt
     4381 gacagatgtt cttacccctg cacaggtaaa acaacgtcag gatgaggaaa atcgcctcca
     4441 gcaggaatgg aatgacacac atcctgtaga agtggctgaa cgcaattatg aacaggcccg
     4501 cgcagaactg gatcaggcta ataaagatgt tgccagaaac caggaacgac aggctaaagc
     4561 tgttcaggtt tataattctc gtaaaagtga acttgatgct gcgaataaaa ctcttgctga
     4621 tgcaaaggct gaaataaaac aattcgagcg atttgcccga gaaccaatgg ctgctggtca
     4681 ccgaatgtgg caaatggcag ggcttaaggc ccagcgggca cagacggatg taaataataa
     4741 gaaggctgca tttgatgctg ctgcaaaaga taagtctgat gctgatgctg cgttaggtgc
     4801 tgcgctggaa cgccgtaagc agaaggaaaa taaagaaaag gactctaagg ataaattaga
     4861 taaggagagt aaacggaata agccagggaa ggcgacgggt aaaggaaaac cagttggtga
     4921 taaatggctt gatgatgcgg gtaaagattc aggagcgcca attcctgatc gcattgctga
     4981 taagttgcgt gataaagaat ttaaaagttt tgacgatttc cggaagaaat tctgggaaga
     5041 agtgtcaaaa gattccgagc ttagtaagca atttaaagac agtaataaga cgaacattca
     5101 aaagggaaaa gcaccttttg caaggaagaa agaccaagta ggtggtaggg aacgctttga
     5161 attacatcat gataaaccaa tcagtcagga tggtggtgtc tatgatatgg ataatatcag
     5221 agtgaccaca cctaagcgac atattgatat tcatagaggt aagtaaaaat ggaactgaaa
     5281 catagtatta gtgattatac cgaggctgaa tttctggagt ttgtaaaaaa aatatgtaga
     5341 gctgaaggtg ctactgaaga ggatgacaat aaattagtga gagagtttga gcgattaact
     5401 gagcacccag atggttcaga tctgatttat tatcctcgcg atgacaggga agatagtcct
     5461 gaagggattg ttaaagaaat taaagaatgg cgagctgcta acggtaagcc aggatttaaa
     5521 cagggctaaa atatgaatgc cggttgttta tggatgaatg gctggcattc tttcacaaca
     5581 aggagtcgtt atgaaaaaaa taacagggat tattttattg cttcttgcag tcattattct
     5641 ggctgcatgt caggcaaact atatccggga tgttcagggc gggactgtat ccccgtcatc
     5701 aacagctgaa gtgaccggat tagcaacgca gtaacccgaa atcctctttg acaaaaacaa
     5761 agcgtgtcag gctgattctg atgcgctttt tttgaaatgt cacaaaaatt ccatgcggga
     5821 gatgagagct aaaatccccg tgcagaactt tccacccagg gcgagaaaac ttgtcatttt
     5881 gacctgttcg cccttcggaa cagtcgaaat cgatcgcgca tcgctttcgt gcatagttat
     5941 gcagccctct aaaaacgatt ctggcgcgtt tttctggttt ggcctggtgt tttcttgtct
     6001 ttttgcgttt tttgcgtcag aaagcgactg agggcgtttt aaggggtgcg tacaacggga
     6061 gttatggtaa atggatcggt ttttcgggaa ggatcgacag gatttgccgt tgggtgtagt
     6121 gtaagcggct gaaaaacaaa cgcccctgaa atcatgtcca gatccggcaa gattcaacat
     6181 gaaatacaga gggcgtgtag gtcgcataac ccggagagaa tcgtaacac
//
