LOCUS       CP122778                5650 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC1737 plasmid unnamed3, complete
            sequence.
ACCESSION   CP122778
VERSION     CP122778.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549614
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 255x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 12:41:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,632
            CDSs (total)                      :: 5,504
            Genes (coding)                    :: 4,587
            CDSs (with protein)               :: 4,587
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 917
            CDSs (without protein)            :: 917
            Pseudo Genes (ambiguous residues) :: 0 of 917
            Pseudo Genes (frameshifted)       :: 710 of 917
            Pseudo Genes (incomplete)         :: 217 of 917
            Pseudo Genes (internal stop)      :: 60 of 917
            Pseudo Genes (multiple problems)  :: 67 of 917
            Pseudo Genes (short protein)      :: 4 of 917
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1737"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            1..192
                     /locus_tag="QDW94_25750"
     CDS             1..192
                     /locus_tag="QDW94_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WHG71530.1"
                     /translation="MTKQEKTAINMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1210..2220
                     /locus_tag="QDW94_25755"
     CDS             1210..2220
                     /locus_tag="QDW94_25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG71531.1"
                     /translation="MEFQELENSACLYWPKELAERAASISAISPLIETQDEFLSILTIS
                     TNKPTSCFDAVRLCNKISPNLFVKHLMVLSDIGGERLHRFFKDLDKIYPDRIMEFNIGN
                     SSYSYQFNSNRAWTTKNLNVEKSRLLQPVSDFTREMLDVCMLILWGGNTINNTNLPTEI
                     ENNCVLGNLIGNKEAIEQFVKERYIMVSRQTGGATANDLGHICEIFIKEKLYKLIDNNI
                     SLDGHHIDGVTHNDKDLTTFDIVAKNTTTKKCVGIEISFQVTTNSTIERKAGLAKSRRD
                     LLNSRGHKIAYVIDGSGNFQRSNAIKTIINHSDMTVNFSDNGINSLATFICETLK"
                     /name="hypothetical protein"
     gene            2217..3464
                     /locus_tag="QDW94_25760"
     CDS             2217..3464
                     /locus_tag="QDW94_25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WHG71532.1"
                     /translation="MIKFVDLFSGTGGIRLGFEQAMKEIGLSTKCVKSSEIDKKACETY
                     KLNFDEESYCDIHDVDIDESFDVLLAGFPCQAFSYAGKQLGFADTRGTLFFEVERIIKK
                     NKPKLCLLENVRGLTSHDKGRTFSTILKSLHNLGYHVEYRLLNTSNFGVPQNRVRIYII
                     ASLEKEVTLTIPNDLGAKDTHNYKPSKGGHDFYTESKVSDILEDSPDESYDCSDWFKQR
                     LEDIFEGDLNKVHGIRLIDTRNGNSLHSWDLGLKGECSNDEIIFMNLLIANRRKHIFGT
                     HQDGKALSKAQIETFFKHSETTNIIKNLIKKGYLKEKNGLYNPVCGNMSFEVFKFLDPN
                     KVSITLTASDANRLGVYHNNRLRRLTPRECARLQGYPDSYILHPKDSMAYKQLGNAVSV
                     PVIKNIFLDIMKNNFI"
                     /name="DNA cytosine methyltransferase"
     gene            complement(3827..4060)
                     /locus_tag="QDW94_25765"
     CDS             complement(3827..4060)
                     /locus_tag="QDW94_25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WHG71528.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4067..4585)
                     /locus_tag="QDW94_25770"
     CDS             complement(4067..4585)
                     /locus_tag="QDW94_25770"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WHG71529.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(4623..5054)
                     /locus_tag="QDW94_25775"
                     /pseudo=""
     CDS             complement(4623..5054)
                     /locus_tag="QDW94_25775"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5263..5586)
                     /locus_tag="QDW94_25780"
     CDS             complement(5263..5586)
                     /locus_tag="QDW94_25780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WHG71533.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(271..355)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          366..923
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.583"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3724..5273)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.649"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5270..5619)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.872"
                     /coverage="99.0"
                     /evalue="9.35e-67"
ORIGIN
        1 gtgactaaac aggaaaaaac cgccattaac atggcccgct ttatcagaag ccagacatta
       61 acgctgctgg agaaactcaa cgaactggac gcagatgaac aggccgatat ttgtgaatca
      121 cttcacgacc acgccgacga gctttaccgc agctgcctcg cacgcttcgg ggatgacggt
      181 gaaaacctct gacacataca gctcccggag acggtcacag cttgtctgtg agcggatgcc
      241 gggagcagac aagcccgtca gggcgcgtca gggggtttcg gggcgaagcc ctgaaccagt
      301 cacgtagcgc tagcggagtg tatactggct taactatgcg gcatcagtgc agattgtatg
      361 aaaagtgccc catgccgggt gtgaaatgcc gcacagatgc gtaaggagaa aataccgcat
      421 caggcgctct tccgcttcct cgctcactga ctcgctacgc tcggtcgttc ggctgcggcg
      481 agcggtatca gctcactcaa agacggtaat gcggttatcc acagaatcag gggataacgc
      541 caaaggaaac atgtgagcaa aaaacaagaa cccggaaaag gccacgccgc tggcgttttt
      601 ccataggctc cgcccccctg acgagcatca cagaaatcga cgctcaagtc agaggtggcg
      661 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      721 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
      781 ggcgctttct catagctcac gctgttggta tctcggctcg gtgtaggccg ttcgctccaa
      841 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
      901 tcgtcttgag tccaacccgg taagacacga cttaacgcca ctgtcagcag ccattggtag
      961 agggtgtaca agaaattgct gatacggagt tcttgaagtg aaggcccgac tgcggctaca
     1021 ctgaaaggac agatttggtg gcagcggtct tgtacgccct gttaccttgg gttagaaagc
     1081 ctgagaaact tctaaccttc gaaaaaccca cgcctgagaa cgtgggtttt ttcgtttacg
     1141 cccgtagaga ttacccatca taggggtgta actcaaggtc tatttggata gttttaccaa
     1201 ggaataatca tggagttcca agaattagaa aatagcgcat gcttatattg gcccaaagaa
     1261 cttgcagaaa gagcagcatc tattagcgcc ataagccctc ttatagagac ccaagatgaa
     1321 tttttatcaa tactaacaat atcaacaaat aagcctacat cttgttttga tgccgtacgg
     1381 ttatgcaata agatatcacc caacctattt gtcaagcact taatggtttt gtcagatatt
     1441 ggaggcgaaa gacttcatcg cttctttaaa gatttagata agatctaccc cgacagaata
     1501 atggagttca atataggaaa tagctcttac tcttaccaat tcaatagcaa tagagcatgg
     1561 acaaccaaga atttaaacgt ggaaaaatca cggctcttgc aacctgtaag tgattttaca
     1621 cgtgaaatgc ttgatgtttg catgctgatc ctatggggag gtaatactat aaacaataca
     1681 aatcttccaa ccgaaataga aaataactgt gttttgggga atttaattgg aaacaaagaa
     1741 gcaatagaac agtttgtaaa agaaagatat ataatggtta gcaggcaaac aggaggtgca
     1801 acggcaaatg atcttggaca catatgcgag atctttataa aagaaaaact ttacaagctt
     1861 attgataaca atatatctct agatggacac catatcgatg gtgtaactca taacgataaa
     1921 gatcttacga cttttgatat tgtagcaaaa aacacaacaa caaaaaaatg tgttggtatt
     1981 gaaataagtt ttcaagttac aacaaactca acaatagaaa gaaaagcagg attagccaaa
     2041 agcagaagag atttgctcaa ttcaagagga cataaaatag catatgtgat tgatgggtct
     2101 ggtaattttc agcgttcaaa tgccatcaaa acaataatta accatagtga catgaccgtt
     2161 aacttttcag ataatggtat aaattcacta gcaacattca tctgcgagac attgaaatga
     2221 ttaaatttgt tgaccttttt tctggcacag gtggtattag acttggtttt gagcaagcca
     2281 tgaaagaaat tggattgtca acaaaatgcg taaaaagctc agaaattgac aaaaaagcat
     2341 gcgaaacata caaacttaac ttcgacgaag aatcatattg tgatatacat gatgttgata
     2401 tagatgaaag cttcgatgtt ttattggcag gatttccttg tcaggcgttc tcttatgctg
     2461 gaaaacaact tggttttgca gacacccgag gcacattatt ttttgaagta gaaagaatca
     2521 taaagaaaaa caagcctaag ttatgtcttt tggagaatgt gcgtggatta acttcacatg
     2581 ataaaggaag aacattttca acaatattaa aatctcttca taatttaggg tatcatgttg
     2641 aatacagact attaaacaca agtaattttg gtgtccctca aaatagggtt agaatatata
     2701 taatagcctc attagaaaaa gaggtaacac ttactattcc taatgatctt ggagccaaag
     2761 atacgcacaa ttacaaacca tccaaaggtg gtcatgattt ttatacagaa tcaaaagttt
     2821 ctgacatact agaggatagt ccagacgagt catatgattg cagcgattgg ttcaagcaac
     2881 gccttgaaga catttttgaa ggtgacctaa ataaagttca tggaatcagg ctaattgaca
     2941 caaggaacgg aaactccttg cactcttggg atcttgggct aaaaggcgag tgctcaaatg
     3001 atgaaataat tttcatgaat ttattaattg caaaccgtag aaagcatatt tttggcacac
     3061 accaagacgg caaggctttg tcaaaagcac aaattgaaac attttttaaa catagcgaaa
     3121 ccaccaacat aataaaaaac ctaattaaaa agggttacct taaagagaaa aacggactat
     3181 acaacccagt ttgcggaaat atgtcttttg aagtattcaa attccttgac ccaaataaag
     3241 tgtcaattac acttacggca tctgatgcca atagacttgg agtctatcac aacaatagac
     3301 tgcgacgcct tacgccaaga gaatgcgcaa gactacaagg ttacccagac tcatatatct
     3361 tacacccaaa agatagcatg gcatataaac aattagggaa tgctgtaagc gtaccagtca
     3421 ttaaaaatat ttttcttgat ataatgaaga ataactttat ataaaccata ttactagcaa
     3481 aactgctcta tggttattta attaaattta acagatttac cataacgtcg cattacgcgc
     3541 accgctaaaa cgcgttcagc gcgatcacgg cagcagacag gtaaaaatgg caacaaacca
     3601 cccgaaaaac tgccgcgatc gcgcctgata aattttaacc gcatgaatac ctatgcaacc
     3661 agatggtaca gaccacatta gcccgtctta atccacttaa cctgccattt ttcatagttt
     3721 caccatccca gtgaaggacc atccagtgtg cgttcctgta tttccggctg gcgttcccgc
     3781 tccagggata actcatgttc gcgttcctgt atcagccgtt cgtcccttat ctcctgttct
     3841 cgctgtataa ctggctcaag cgttctgtcc gctcgctcaa gtgctgcacc tgctgactca
     3901 actgcatgac ctgctcgttc agcatcgcgt tgtcccgttg cgtaagcgaa aacattttct
     3961 gcaattccac gaaggcgctc tcccattcgt tcagccgctg catatagtcc tgttgcagtt
     4021 gctctaatgc gttcagcaaa tgtctttcca gctccgtcac tctgtgtcac tccgtcagtt
     4081 gtacccagtc cttcccctga tagggaatca ccgttgccga tttcccctgc gggatgacca
     4141 gaaatttcct tctcccgtcc tgcacaaact ccacgcccca tgtcttcgcg ttcagtttct
     4201 gcaatgtctc ttcctgcttc ctgatttctt ccaggttcgt ctgtatccgc ccgccaagat
     4261 accagagcgt cccgccactc acggtaaaca ggaaaatgac catccccagt aacatcatgc
     4321 ccgtattccc tgccagcttt aacacgtcct tcctgtgctg catcatcgcc tctttcaccc
     4381 cttcccggtg ttttttcagt gattcctctg tcgaagctgt gaacagggct atagcgtctt
     4441 ttattttcgt ctcgtttgag gtcacagcct tgcttacaga tttttcgagc ttgctgaact
     4501 cgtcgttcag cattgtctct gtagattcgg ctctctcttt cagctttttc tcgaactccg
     4561 cgcccgtctg taaaagattg ctcatagaat gctcctttca gcctgatatt cttcccaccg
     4621 ttcgggtctg caatgctgat actgcttcgc gtcaccctga ccacttcaag ccctgcctct
     4681 gtgagcgcct gaatcacatc ctgacggcct tttatctccc cgacatgata aagagcgtct
     4741 atcccgcgtg tgacgctctc tgcaagcgcc tgtttcgttt ccggcaggtt atcagggaga
     4801 gtcagtatcc ggtggttctc cggggcgttg gggtcatgca gcccgtaatg gtgattcacc
     4861 agcgtctgcc aggcatcaat tctcggcctg tctgcccggt cgtagtacgg ctggaggcgt
     4921 tttccggtct gtagctccat gttcggaatg acaaaattca gctcaagccg tcccttgtcc
     4981 tggtgctcca cccacaggat gctgtactga ttcttttcga gaccgggcat cagtacacgc
     5041 tcaaagctcg ccatcacctt ttcacgtcct cccggcggca gctccttctc cgcgaacgac
     5101 agaacacctg acgtgtattt cttcgcaaat ggcgtggcat cgatgagttc ccggacttct
     5161 tccggattac cctgaagcac cgttgcccct tcccggtttc gctcccttcc cagcaggtaa
     5221 tcaaccggac cactgccacc gccttttccc ctggcatgaa atttaacaat catcccgcgc
     5281 gccctgttcc ctgacggcca gacgcagccg acgcagctcc tccccgatgg ccatcagcgc
     5341 agccaacacc tgaacccgat caccggaaga ccactgcccg ctgttcacct tcctcgctgt
     5401 ctgattcagg ttattcccga tggcggccag ctgacgcagt aacggcggcg ccagtgtcgg
     5461 cagtttcccg gaacgggcaa ccggctcccc caggcagacc cgccgcatcc ataccgccag
     5521 ctgtttaccc tcacagcgtt caagtaaccg ggcatgttca tcatcagtaa cccgtattgt
     5581 gagcatcctc tcgcgtttca tcggtatcat taccccataa aacagaaatc ccccttacac
     5641 ggaggcatca
//
