LOCUS       CP122776                5261 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC1737 plasmid unnamed1, complete
            sequence.
ACCESSION   CP122776
VERSION     CP122776.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549614
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 255x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 12:41:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,632
            CDSs (total)                      :: 5,504
            Genes (coding)                    :: 4,587
            CDSs (with protein)               :: 4,587
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 917
            CDSs (without protein)            :: 917
            Pseudo Genes (ambiguous residues) :: 0 of 917
            Pseudo Genes (frameshifted)       :: 710 of 917
            Pseudo Genes (incomplete)         :: 217 of 917
            Pseudo Genes (internal stop)      :: 60 of 917
            Pseudo Genes (multiple problems)  :: 67 of 917
            Pseudo Genes (short protein)      :: 4 of 917
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1737"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            1..342
                     /locus_tag="QDW94_25015"
     CDS             1..342
                     /locus_tag="QDW94_25015"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WHG71428.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(339..1907)
                     /gene="cea"
                     /locus_tag="QDW94_25020"
     CDS             complement(339..1907)
                     /gene="cea"
                     /locus_tag="QDW94_25020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WHG71429.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2167..2604
                     /locus_tag="QDW94_25025"
     CDS             2167..2604
                     /locus_tag="QDW94_25025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WHG71430.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            2685..3103
                     /locus_tag="QDW94_25030"
                     /pseudo=""
     CDS             2685..3103
                     /locus_tag="QDW94_25030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            3872..4066
                     /locus_tag="QDW94_25035"
     CDS             3872..4066
                     /locus_tag="QDW94_25035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WHG71425.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4721..4879
                     /locus_tag="QDW94_25040"
     CDS             4721..4879
                     /locus_tag="QDW94_25040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WHG71426.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(5077..5214)
                     /locus_tag="QDW94_25045"
     CDS             complement(5077..5214)
                     /locus_tag="QDW94_25045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WHG71427.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     Typing          complement(3364..3608)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.276"
                     /coverage="88.0"
                     /evalue="7.199999999999999e-94"
     Typing          complement(3738..3836)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(4140..4222)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          4248..4793
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.536"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 atgagcttaa gatactacat aaaaaatatt ttatttggcc tgtactgcac acttatatat
       61 atatacctta taacaaaaaa cagcgaaggg tattatttcc ttgtgtcaga taagatgcta
      121 tatgcaatag tgataagcac tattctatgt ccatattcaa aatatgctat tgaacacata
      181 gcttttaact tcataaagaa agattttttc gaaagaagaa aaaacctaaa taacgccccc
      241 gtagcaaaat taaacctatt tatgctatat aatctacttt gtttggtcct agcaatccca
      301 tttggattgc taggactttt tatatcaata aagaataatt aaatccctaa cacctcattt
      361 atagtattaa gtttattctt atcaatatag gagcatagaa ttcctgtaac aatagcaata
      421 ccccaaatac ctaatgtagt tccagcaagc aagctaaaaa gtaaagcaac aacataactc
      481 acccctgcat ctgcagcttt cttctctaat gtaagaaata gtggcttcca gtcacctgta
      541 tccttaattt ttaggatatc agataccaca tcatatccaa aagaaacatg ccccgtaatc
      601 ttcaagtact tggcaaactg atctaaatgt ttagcccagt catcatactt caccgatgcc
      661 aacgcattaa aaatagcatc acgatcggct ttgctgaatt tcttatttaa aacatccttg
      721 tatttttcaa aagcagcgag agcttcattc acattgccga ttttcttacc tttagactta
      781 tcagcaagtt cctgtgccat tttcgaatat ttttcaccat atttttcagt cagcgtttga
      841 taaaagctaa ctgttgcatc aacagcatcc ttaatctgtg aattaaggag attattctgt
      901 gcttttttca aattttcttc agcttcatga acacgagcga taccggcatt acgattatta
      961 ctgacctgag aaatagcctt ctggatctga gttatatcag catttatccg gttaatacgt
     1021 gtttctgatg ctgttacctg tttttgtttt tcttctctaa tcttaccggc cccaacccgt
     1081 cgtctggttg cttcaaaaaa aggacggttc tgaagcggat cattggctct tggtgatagt
     1141 tttttgacca gctcatccag ttctttatat ttagcggatg cctgagccag ttcatttcgt
     1201 tttccagcga gcgttttcat ttctgcatca cgggcatgga tactggagct taaacgagaa
     1261 ttgagagtct taatctctcc atccattttc accacttcag attgtgcagc agaaagtttt
     1321 ttttgggcga tctcaacagc tttagcttct tcactcaatg cagccagtcg tttctcttca
     1381 gcttcagcca atttcaactg gcgttctgtt tcagccttct cccgttcaat ctctttacgt
     1441 cgttgttctg cttcctgaaa agccttttct gctgcttccg cttctttacg ggctttttct
     1501 tctgctttcg caaggcgcaa acgctctgct tccgcctgca tagctgcatt attagcatga
     1561 gcaagctctg ttgctgaagg cgtacgtgag gcattgtgac gaagagcctc attcacgata
     1621 tccttcaggc gctgagtcag cgcatccctg tttgcctttg ctttcgcctg tgcttccgct
     1681 gcagcttttg cccgggcagc ctgctctgcc tgtgttttct ttaattgagc agtagaccat
     1741 ttagcagttg catgaatagc tgcagaactt tcacttttac tgcctccttt tccacctccg
     1801 ccgccagagc cactcccgtc aggagtacca ttcaaaagag taataattac ctgtccctta
     1861 tcatcataag gaacaccatc tttatagtac gctaccgcgg tttccattat aaaatcctct
     1921 ttgactttta aaacaataag ttaaaaataa atactgtaca tataaccact ggttttatat
     1981 acagcataaa agctacgccg ctgcattttc cctgtcaaga ctgtggactt ccatttttgt
     2041 gaaaacgatc aaaaaaacag tctttcacac cacgcgctat tctcgcccga tgccacaaaa
     2101 accagcacaa acattaccgt tctcagacct cattatgttt tactgaaact atgagatgag
     2161 acatctatgg gacactgtca ctttatggca tggcacacac tccgggacgc actaaaaatg
     2221 acaggcagat cgcgttcaca gttttaccgt gatatgcgcg caggccttgt cagttaccgt
     2281 accggcaggg acggacgacg ggagtttgaa accagtgaac tgatccgggc atacggcgaa
     2341 ttaaagcaga atgagacacc agaaaggcac agtgagggac atgcagaaaa tccacatgat
     2401 cagcagacag aacgcattct ccgggagctg aatgagctga aacaatgcct gacgctgatg
     2461 cttgaggata aacaggcaca ggatatggat cgcagacgcc aggaagcaga acgggaacag
     2521 ctacaaaatg agatagccca gctcaggcag gcactggaac tggaaaagaa acggggattc
     2581 tggtccaggt tgttcggtcg ctgaacgctg tcagagactg atgataaaat agtcttcgga
     2641 taataactca ccgagaataa atactttaag gtagggagac actcatgaga cgtaccggaa
     2701 acaaactttg tcttatcgcc atgataacag caacagtagc tctcacagcc tgtaccccaa
     2761 agggcagcgt ggaacaacat acccggcatt acgtatatgc ttctgatgac ggttttgatc
     2821 ccaacttttc cacccaaaaa gccgacacaa cacgaatgat gatgcctttt ttcggcagtt
     2881 ctgggatatg ggagctaaag ataaagcgac aggaaaatca cggagtgatg tgcaacaacg
     2941 cattcagcag tttcacagcc aggaattttt aaactcactc cggggcacaa ctcaatttgc
     3001 gggtactgat taccgcagca aagaccttac cccgaaaaaa tccaggctgc tggctgacac
     3061 gatttctgcg gtttatctcg atggctacga gggcagacag taagtggatt taccataatc
     3121 ccttaattgt acgcaccgct aaaacgcgct gaacgcgatt ctgccgcaaa caccgcaaaa
     3181 agagatgttt tccccggggt aaaccggaaa aatgcgtcag gatcgctttc agctcactgc
     3241 atagctatgc atgaaagtga atgacgatcg gtttgggcgc atacagtgcc catgagacac
     3301 attttcggac attttcagtt cgtgaatccc tgactcattg ctatatatct gaatagacac
     3361 ccctagcagc gcccccaggg gcatcctgta aaaaaacaca ccgcgccgct agcagccccc
     3421 ctaatataaa acgatgtttt ttatgaaaat agtcagtacc acccctacaa agcggtgtcg
     3481 gcgcggtgtt gtagctgcgt taacgacgct gttttaaata aatcagattt aaacaatata
     3541 aatccacaaa cataactcaa tgatattaaa gaaaaatcag caaaaagtta gttttgcgtg
     3601 gggtgtggca tttcaggcat gagaacagac tcgcgttttt ccggagtatc agcggaatta
     3661 ttgattaaga aacactcgac gcagagcgaa attttatgca acagcggctg cactgcacag
     3721 ggattattta tcagttgata tgctgtgcca tgatacgtcc taaaaaaaca ggacaggatg
     3781 tgcaattgta ataccgtcgc ccgcaacgtc ttacaattac cacctggtca gggtccaccc
     3841 gacacccggt actgacggag aacaacggaa ggtgaacaaa cagcaacaaa ctgcgctgaa
     3901 tatggcgcga tttatcaaaa gccagagcct gacactgctt gaaaaactgg atgctctgga
     3961 tgccgacgaa caggcggcca tgtgcgaacg actgcacgaa ctcgcggaag aactccagaa
     4021 cagcatccag gttcgctttg agaccgaaag tgaaacagga acataacgaa gctgacggag
     4081 gcgctcacgg cttgtccgcg agcgttttcg gcagcagaca agcccgcctg ggcgcgtcag
     4141 cgggtgtcgg ggcgaagccc tgacccagtc acgtagcgat agcggagtgt atactggctt
     4201 aactatgcgg catcagtgca gattataaga aagggcgacc atatgcggtg tgaaataccg
     4261 cacagatgcg taaggagaaa atgccgcatc aggcgcactt ccgcttcctc gctcactgac
     4321 tcgctacgct cggtcgttcg actgcggcga gcggtactga ctcacacaaa aacggtaaca
     4381 cagttatcca cagaatcagg ggataaggcc ggaaagaaca tgtgagcaaa agaccaggaa
     4441 caggaagaag gccacgtagc aggcgttttt ccataggctc cgcccccctg acgagcatca
     4501 caaaaataga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4561 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4621 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta
     4681 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4741 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taaggcacgc
     4801 cttaacgcca ctggcagcag ccactggtaa ccggattagc agagcgatga tggcacaaac
     4861 ggtgctacag agttcttgaa gtagtggccc gactacggct acactagaag gacagtattt
     4921 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4981 ggcaaacaaa ccaccgttgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     5041 agaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga tccccgtcag
     5101 tttagaagag gaggatggtg cgatggtccc tccctgaaca tcacgtatat agttagcctg
     5161 acatccaaca aggaggttta tcgcgaatat tcccacaaaa aatcttttcc tcataactcg
     5221 atccttataa aatgaaaaga atatatggcg aggtttaatt t
//
