LOCUS       CP122757                5131 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC1743 plasmid unnamed6, complete
            sequence.
ACCESSION   CP122757
VERSION     CP122757.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549620
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 155x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 12:25:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,696
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,111
            CDSs (with protein)               :: 5,111
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 454
            CDSs (without protein)            :: 454
            Pseudo Genes (ambiguous residues) :: 0 of 454
            Pseudo Genes (frameshifted)       :: 305 of 454
            Pseudo Genes (incomplete)         :: 147 of 454
            Pseudo Genes (internal stop)      :: 53 of 454
            Pseudo Genes (multiple problems)  :: 47 of 454
            Pseudo Genes (short protein)      :: 4 of 454
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1743"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            join(4251..5131,1..4)
                     /locus_tag="QDW98_28445"
     CDS             join(4251..5131,1..4)
                     /locus_tag="QDW98_28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WHG87452.1"
                     /translation="MAVTVGFISEKGGVGKTTSCYHIAEGLSRFHEKRVLVIDADYQRG
                     GITGRFFPKLIENFGNQAPQGTTLFNKYQQLYSAAQQTTDIDIIKFNSGIDVIPADPRL
                     STVSTDKLPSTNNIRSNNAILLQHLKTISAVLSPIADNYDYILIDSHPEVSDVMRSIIY
                     ASDYCVSPVKLDRQSSIGVATVIGEINNVNEDIEMLRKALQVGDSYKDTVFAGAMGMMA
                     REYAEELKQTEQYEYNRLRQAGDIFKHYITEGDGLRVAAAERVSVYDVQISNAYKQASQ
                     FKNLTIEFMRTCR"
                     /name="ParA family protein"
     gene            1..402
                     /locus_tag="QDW98_28450"
     CDS             1..402
                     /locus_tag="QDW98_28450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG87453.1"
                     /translation="MIDIQKLISWLGVEGAKAGLDKSEMTNAELIESFGNLLPKNPSKL
                     KRSDLVEEIILATRRMTHKSVEELMEMSKEDLYSYFHDQKYSRKELLDLLYTLEIRPGS
                     SAKKNLTEFTISEISDIGMYRRVAKGNHA"
                     /name="hypothetical protein"
     gene            complement(1222..1455)
                     /locus_tag="QDW98_28455"
     CDS             complement(1222..1455)
                     /locus_tag="QDW98_28455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WHG87454.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1462..1980)
                     /locus_tag="QDW98_28460"
     CDS             complement(1462..1980)
                     /locus_tag="QDW98_28460"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WHG87455.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVLPYGKSATVIPYQ
                     EKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(2018..2449)
                     /locus_tag="QDW98_28465"
                     /pseudo=""
     CDS             complement(2018..2449)
                     /locus_tag="QDW98_28465"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2658..2981)
                     /locus_tag="QDW98_28470"
     CDS             complement(2658..2981)
                     /locus_tag="QDW98_28470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WHG87456.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3046..3237
                     /locus_tag="QDW98_28475"
     CDS             3046..3237
                     /locus_tag="QDW98_28475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WHG87450.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3871..4002
                     /locus_tag="QDW98_28480"
     CDS             3871..4002
                     /locus_tag="QDW98_28480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WHG87451.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHDKAPLAAAMSKGVYKIY"
                     /name="replication initiation protein"
     Typing          complement(3333..3506)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.0"
                     /coverage="100.0"
                     /evalue="5.699999999999999e-63"
     Typing          3430..4019
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.107"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(1119..2668)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgatagata tccaaaaact gatatcttgg cttggcgttg agggagctaa agctggcttg
       61 gataaaagtg aaatgactaa tgccgagcta attgaatcct ttggtaattt acttccaaag
      121 aacccaagca aattaaaacg ctccgatctc gttgaggaga ttattttagc aacaaggaga
      181 atgacacata aatcagttga agaactgatg gaaatgtcaa aagaagatct gtattcatat
      241 ttccacgatc agaaatatag cagaaaagaa cttcttgatt tgttatatac tcttgaaatc
      301 agacctggga gctcagcaaa gaaaaatcta accgagttca caataagtga gataagtgat
      361 attggcatgt atcgtcgcgt agcgaaaggg aatcatgcat aaataaaagc cacctgtgag
      421 gtggcttttt ataggataaa aaaatgccat ttaatatagg cgatatagtt tacttaaagt
      481 cagaatcaaa agcgatgacg gtcatcggat atggatcgtc tcaaaataac ggttacatag
      541 atctagctaa ttgtgcttgg ctagataaag acaaccgcta ttgccaaata ctattcttgt
      601 ttcaaacgat cgaaaaaaga atcagttaaa aactggctta tcttcattga gcataccgga
      661 cgaacctatt taccataaca tccgttgccc gcaccgcaga aacgcgttcg gtgcgatcac
      721 ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa actgccgcga tcgcacccgg
      781 taaattttaa ccacatgcat agctatgcag ccatgtgaat cacgcaggaa ttgccagcca
      841 gagacagctg aaacggattt ttttcatgct ttcggaagcg aagaagacgg ggaccggagc
      901 gggaaaacag aagttcaggg gaatgagcgc gatctggcaa tagaggcaaa acacagcaac
      961 aaaagacaca ccagaatcgc gcccgtatgc gttttaacgc gttcctgtgt tttcagcaag
     1021 gtctgactga agcgcgttcc cggtgccccc gaaccaacca ttatcccaaa aaatgcaccg
     1081 gaaacgcgct gacaacattt tagcctgtct ctgattacca tccaagcgaa gggccatcct
     1141 gtgtccattc ctgtatttcc ggctggcgct cacgctccag ggataactca tgttcgcgct
     1201 cctgtatcag ccgttcgtcc cttatctcct gttctcgctg tataactggc tcaagcgttc
     1261 tgtccgctcg ctcaagtgct gcacctgctg actcaactgc atgacctgct cgttcagcat
     1321 cgcgttgtcc cgttgcgtaa gcgaaaacat tttctgcaat tccacgaagg cgctctccca
     1381 ttcgctcagc cgctgcatat agtcctgttg cagttgctct aaggcgttca gcaaatgtct
     1441 ttccagctcc gtcactctgt gtcactccgt cagttgtacc cagtccttct cctgataggg
     1501 aatcaccgtt gccgatttcc cgtacggaag gaccagaaat ttccttctcc cgtcctgcac
     1561 aaactccacg ccccatgtct tcgcgttcag tttctgcaat gtttcttcct gtatcctgat
     1621 ttcttccagg ttcgcctgta tccgcccgcc aagataccag agcgtcccgc cactcacggt
     1681 aaacaggaaa aggaccatcc ccagtaacat catgcccgta ttccctgcca gctttaacac
     1741 gtccttcctg tgctgcatca tcgcctcttt caccccttcc cggtgttttt tcagtgattc
     1801 ctctgtcgaa gctgtgaaca gggctatagc gtctttgatt ttcgtctcgt ttgatgtcac
     1861 agccttgctt acagattttt cgagcttgct gaactcgtcg ttcagcattg tctctgtaga
     1921 ttcggctctc tctttcagct ttttctcgaa ctccgcgccc gtctgtaaaa gattgctcat
     1981 agaatgctcc tttcagtctg atattcttcc cgccgttcgg gtctgcaatg ctgatactgc
     2041 ttcgcgtcac cctgaccact tcaagccctg cctctgtgag cgcctgaatc acatcctgac
     2101 ggccttttat ctccccgaca tgataaagag cgtctatccc gcgtgtgacg ctctctgcaa
     2161 gcgcctgttt cgtttccggc aggttatcag ggagagtcag tatccggcgg ttctccgggg
     2221 cgttggggtc atgcagcccg taatggtgat taaccagcgt ctgccaggca tcaattctcg
     2281 gcctgtctgc ccggtcgtag tacggctgga ggcgttttcc gctctgtagc tccatgttcg
     2341 gaatgacaaa attcagctca agccgtccct tgtcctggtg ctccacccac aggatgctgt
     2401 actgattttt ttcgagaccg ggcatcagta cacgctcaaa gctcgccatc accttttcac
     2461 gtcctcccgg cggcagctcc ttctccgcga acgacagaac acctgacgtg tatttcttcg
     2521 caaatggcgt ggcatcgatg agttcccgga cttcttccgg attaccctga agcaccgttg
     2581 ccccttcccg gttacgctcc cttcccagca ggtaatcaac cggaccactg ccaccgcctt
     2641 ttcccctggc atgaaactta actatcatcc cgcgctccat gttccctgac gacctgccgt
     2701 aagctgcgca actctctctc gatggccatc agtgcggcca ccacctgaac ccggtcaccg
     2761 gaagaccact gcccgctgtt caccttcctc gccgtctgat tcagattatt cccgatggca
     2821 gccagctggc gcagtaacgg cggtgccagt gtcggcagct tcccggaacg ggcaaccggc
     2881 tcacccagac agacccgccg catccatacc gccagctgtt taccctcaca gcgttccagt
     2941 aaccgtgcat gttcatcatc agtaacccgt atcgtgagca tcctctcgcg tttcatcggt
     3001 atcattaccc catgaacaga aatcccccct tacacggagg catcagtgac taaacaggaa
     3061 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct gctggaaaag
     3121 ctcaacgaac tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgcc
     3181 gatgagcttt accgcagctg cctcgcacgc ttcggggatg acggtgaaaa cctctgacac
     3241 atacagctcc cggagacgga cacagcttgt ctgtgagcgg atgccgggag cagacaagcc
     3301 cgtcagggcg cgtcaggccg ttgaccttgc gggtgtcggg gcgcagccat gacccagtca
     3361 cgtagcgata gcggagtgta tactggctta ctatgttggc atgagtgcgg gtatcaggaa
     3421 agtgcttcat gtggcaggag aaaaaaggcg gcaccggagc gtcagcagaa tatgtaatac
     3481 aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg actgcggcga
     3541 gcggaaatgg cttacgaacg ggaagcggag atttcctgga agatgccagg aagatactta
     3601 aacagggaag cgatagggtc gcggcgaagc cgtttttcca taggctccgc ccccctgaca
     3661 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     3721 accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     3781 ccggtgtcat tccgctgtta tggccgcgtt tatctcattc cacgcctgac actcagttcc
     3841 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgactgc
     3901 tgcgcctgtt ccggtaacta tcgacttgag tccaacccgg aaagacacga caaagcgcca
     3961 ctggcagcag ccatgagtaa aggggtgtac aagatttact gagtgagagt tcttgaagta
     4021 tcagccttag agaggctaaa ctggaataac agaatttggt ttcagcggtc ttgtacaccc
     4081 tgttaccacg gttcagaagt tccccaactt actgaaccct cgaaaaaacc gactccccag
     4141 tcggtttttt tcgtttgatg gctaagagta cgctgacgtt atactgaacg tcaaggaaag
     4201 cgttgacgct gttccctcta ccaaaaaaaa gagatttgta aggaataaaa atggctgtga
     4261 cagttgggtt tattagtgag aaaggtgggg ttggtaaaac aacgtcctgt tatcacattg
     4321 ccgaaggact tagtcgtttt catgaaaaac gtgttttagt tatagatgca gattatcaac
     4381 gcgggggtat aactggaaga tttttcccta aactgatcga aaactttgga aatcaagcac
     4441 cgcaaggaac aacgttattt aacaagtatc agcaattata tagcgcggca caacaaacga
     4501 ctgacataga cataattaaa tttaatagtg gtatcgatgt cattccagcc gaccctcgct
     4561 tatcaaccgt ttcaactgat aagctaccta gtacaaacaa tataagatcg aacaatgcca
     4621 ttcttttaca gcatttaaaa accataagcg ctgtattatc acctattgca gataattatg
     4681 actacatatt gattgattca caccccgaag tttctgatgt catgcgctct ataatctacg
     4741 catcagatta ttgtgtatca ccggtaaaac tagacaggca atcatctatt ggtgtggcca
     4801 cggtcatagg tgagataaat aatgtaaatg aagatattga aatgctaaga aaggcactgc
     4861 aagtcggcga ttcatataag gacacagttt tcgccggagc aatgggaatg atggccagag
     4921 aatatgcaga agaactgaag caaacagagc aatatgaata caacaggctt cggcaagcag
     4981 gtgacatctt caagcactac ataacggaag gtgatgggct aagagtagct gcagcagagc
     5041 gcgtttcagt gtatgatgta cagataagta acgcttacaa acaagcgtca cagtttaaga
     5101 acttgacgat tgagtttatg aggacttgtc g
//
