LOCUS       CP122752                9450 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC1743 plasmid unnamed1, complete
            sequence.
ACCESSION   CP122752
VERSION     CP122752.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549620
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 155x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 12:25:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,696
            CDSs (total)                      :: 5,565
            Genes (coding)                    :: 5,111
            CDSs (with protein)               :: 5,111
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 454
            CDSs (without protein)            :: 454
            Pseudo Genes (ambiguous residues) :: 0 of 454
            Pseudo Genes (frameshifted)       :: 305 of 454
            Pseudo Genes (incomplete)         :: 147 of 454
            Pseudo Genes (internal stop)      :: 53 of 454
            Pseudo Genes (multiple problems)  :: 47 of 454
            Pseudo Genes (short protein)      :: 4 of 454
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1743"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed1"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            1..273
                     /locus_tag="QDW98_26335"
     CDS             1..273
                     /locus_tag="QDW98_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG87081.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSCT
                     VCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
                     /name="hypothetical protein"
     gene            254..1463
                     /locus_tag="QDW98_26340"
     CDS             join(254..1051,1053..1463)
                     /locus_tag="QDW98_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0004803 - transposase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WHG87082.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLA
                     CGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVH
                     LVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSHITTE
                     SQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFR
                     YLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGM
                     DKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNARDISLLRY
                     MPA"
                     /name="IS91 family transposase"
     gene            complement(1857..2441)
                     /locus_tag="QDW98_26345"
     CDS             complement(1857..2441)
                     /locus_tag="QDW98_26345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WHG87083.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(2454..3551)
                     /gene="mobA"
                     /locus_tag="QDW98_26350"
     CDS             complement(2454..3551)
                     /gene="mobA"
                     /locus_tag="QDW98_26350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WHG87084.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     DTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQR
                     QQEKEQQRLAEQTRQKPDKGWSFSR"
                     /name="mobA"
     gene            3717..4184
                     /gene="traD"
                     /locus_tag="QDW98_26355"
     CDS             3717..4184
                     /gene="traD"
                     /locus_tag="QDW98_26355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WHG87085.1"
                     /translation="MSKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     gene            4776..5726
                     /locus_tag="QDW98_26360"
     CDS             4776..5726
                     /locus_tag="QDW98_26360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003887 - DNA-directed DNA polymerase activity [Evidence
                     IEA]; GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WHG87076.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            5734..6147
                     /locus_tag="QDW98_26365"
     CDS             5734..6147
                     /locus_tag="QDW98_26365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG87077.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            6394..6711
                     /locus_tag="QDW98_26370"
     CDS             6394..6711
                     /locus_tag="QDW98_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WHG87078.1"
                     /translation="MFNVILHDDVPNELAGLPPVVRAKMIRLIDKLKTNATALREPDSK
                     PLRDGLFELRTMGTDIARGLYVYQKGRNIYLLRVFIKKTQKTPSGEISMAFERLKEMLN
                     E"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            6704..6991
                     /locus_tag="QDW98_26375"
     CDS             6704..6991
                     /locus_tag="QDW98_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WHG87079.1"
                     /translation="MSKVKGIAWEHVRNELMSNPEVQAAYEAEERKERLQAMLAEWRHH
                     AGLTRAQVAERMGVTPPTVSRMESNVIKASIETLARYARACGVKHPQITL"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(7329..8054)
                     /locus_tag="QDW98_26380"
                     /pseudo=""
     CDS             complement(7329..8054)
                     /locus_tag="QDW98_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011638903.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase activity
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /name="sulfatase-like hydrolase/transferase"
     gene            8128..9108
                     /locus_tag="QDW98_26385"
     CDS             8128..9108
                     /locus_tag="QDW98_26385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WHG87080.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQNPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     ARG             complement(7330..8096)
                     /name="MCR-4.6"
                     /gene="MCR-4.6"
                     /gene_name="MCR phosphoethanolamine transferase"
                     /tool="rgi"
                     /drug_class="peptide antibiotic"
                     /identity="100.0"
                     /coverage="47.13"
     Typing          5551..5726
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(2458..3551)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtcacaca acctggagca ccagaaagtc cacaccagaa tggtgaaaga ggttctgaaa
       61 gccgtcgcca gggccaataa tcacccttat cagtctgtat ttacagactt tatcgccggt
      121 catccctcct gtacggtctg tttctgggaa accttccaca aaatgtaccc ggattcaccg
      181 tacgaatacg tcacgttctg ccatacctgc cgtcgttttg atttatacga aactgaagcg
      241 gagatgaagg cggatgaccc gaagtggtgg tgattttcag ccccgaccgc tgaaacgcct
      301 gttcacagcc aaccagtgct ggacatcctt cctggatgcg ggcggcctgc gcgatatcga
      361 ggttgaggct gtcacaaaaa tgctggcctg cggcacgcgg atactgggtg tgaaggaata
      421 caactgcgat aaacctgagt gcccccacgt cagatacgtc actaactcat gtggcagccg
      481 tgcctgcccg tcctgcggaa aaaaggccac agacctgtgg atagcgacac agctgaatcg
      541 tcttcctgac tgcgactggg tgcacctggt cttcaccctg ccggacacgc tgtggccggt
      601 gttcgaaagc aaccgctggc tgctgaatga cgtgtgccgt ctggcggtgg agaatctgct
      661 gtatgcagcc cggaaacggg gtctggaacc cggtatcttc tgcgccatcc atacgtatgg
      721 ccgtcgtctc aactggcatc cgcatgtgca tgtgtcagtg acctgcggag gtctgaataa
      781 acacggacac tggaaaaagc tgagcttcct gaaagacgcg atgcgttcgc ggtggatgtg
      841 gaatatgcgg cagctgcttc tgaaagcgtg gtcagaaggt ctggcgatgc cggaatcgtt
      901 gtcacatatc acgacggaat cacagtggag aagcctggtg ctgaaagccg gcggaaaata
      961 ctggcatgtg tacatgtcga agaagacagc cggagggcgg aatacggcac gctacctggg
     1021 tcgttatctg aagaagccac caatagcggc tttcccggct ggcgcattac aacggaggag
     1081 cgagtctgag cttccgttac ctggaccaca aaacgggaga aacggcgacg gaaacgctga
     1141 cacagcggga gctggtcgcg aggctgaaac agcacatccc ggagaagttt tttaagatgg
     1201 tgagatactt cgggttcctt gccaaccggg tgtgtggaga gaagctgccg caggtgtacc
     1261 gtgctctggg gatggataag ccggaaccag tggcgaaagt gtgctatgca caaatggtga
     1321 aacagttcct gagtcgtgac ccgttcgaat gcgtgctgtg tggcggccgg atggtatacc
     1381 gccgggccat tgcggggctg aatgtatcag ggctgaagaa aaacgcgcgg gatatcagtc
     1441 tgctgaggta tatgccggcc tgacacaggc taagtgtgcc cgggaatcgg tgcaggtata
     1501 aaaaacagcc tgttacggta taaaaatcga ccatgcagag agagaaacca ccggagtgga
     1561 gggaaaagca gaaaggctca cgtggcataa ggcgtggaca gcggtatcgt ctggtttttc
     1621 aatttcctat acgtgaacct cacgcgcgtc tttcatcagg gcccgaatcg atgacgattc
     1681 atcatggccg tttatagtac cgcagtctct cccctttatt tttactgaag agcagacaga
     1741 aaccataaat accggctttg acatgccggg gaagtcctta tagtacaata attctctata
     1801 tccaaactga ccccctaatt cacgacaaca ataacggcgc aatgtgcgcc gttatgttat
     1861 tttttgataa tcatccaggt attctcttta tcaccatacg tagctcgggt gtccacctta
     1921 acgcagggct ttccgtcaca acgcgatata tcagccagcg gggctttctt ctgccagacc
     1981 gcttccatcc gttgcatttc agcaatctgg ctgtacccgt cattcataaa ccacgtaaat
     2041 gccgtcacgc aggcagccat gacgaaaaat atcgtcagca cgagataaat cgcggatttc
     2101 cacgtatagc gcgacatctc gcgtcgcatt tcatggatca tcgccttcgc cgtatcggta
     2161 gcctgattaa gcgcttctgt cgccggtttc tgctgtgcta atccggcttg tttcagctct
     2221 ttctcaacct gaatgagcgc ggcatgaaca gattgcctga cggtttcagt catattaccg
     2281 gacgcgctgt ccagctcacg aatgagcctg ctcagcgtgt cactctgctg ctgtaattgt
     2341 gctgaggcgt cctgaaactg ctcagtcaga gaggtaacac gcttttccag cgcctgatga
     2401 tgcccgatga gggcagcgat ttgtttaatt tcgtcgctca tgaaaaatcc tgtctatcgt
     2461 gaaaatgacc agcctttatc cggcttctgt cgtgtctgtt cggcgagtct ctgctgttct
     2521 ttctcctgct gacgctgttt ttctgccagt cgctccattt cctgatgtat cccctgaaac
     2581 tccgccattg tctcgttcac aagggactga aggtcgattt cgtcctgtac gtccttcagc
     2641 gcatcactga ccagtgcgtt cagcttctcc ggctctttct caaaatcgaa aaccctgccg
     2701 gaagtgtctt cctgcgcagg ttcaggcttc tgctcttgtt ttatcgtcag gttatcccgc
     2761 tcgctgatgg cttcccgtag cgaggtggtc acgtcaatca cactgtcacg ttcatcacga
     2821 gactgctccg cctgcctttc agcgtccctg cgctcaagga tggcctgtaa ctgcgcagta
     2881 tcgaaccgct gaacctgtcc cgcgccaaga tgccgctcag gctcacgctc aatgccctga
     2941 gctttgagtg agcgtgcatc aacgcggtcg gtatgctgat accgttcaag gtgcttattc
     3001 tgtaggtcag cccagcgttc ccgctgggcg acgaggtgct ctttgcgttc tgtcggcgtt
     3061 tccccgaaac gtgccttttt cgcaccgccg cgctcagggg ctttgctgtt agctcgctta
     3121 aaatactgct gcgggtcacg gtcgataccg tcattcatcc gctcagagaa catgatatgc
     3181 gcgtgcggct gctcgccgcc ggctatcgcc gctttcggat tatgaatggc gaactgatac
     3241 gcatgacggt cgccgatttc ctgccggaca aaatcgcgga ccagttcaag gcgttgttcg
     3301 ggtttcagct cacgcggcag ggcaatctcg atttcacggt aggtacagcc gttggcacgt
     3361 tcaaacgtgt cagcggcttt ccagaactcc gtcggtttat gcgctgccca ggcaggcata
     3421 ttgccggact ccttgtgctc aaggtcggag tctttttcac gggcatactt tccctcacgc
     3481 gaaatataat cggcatgggg agaggcactg cctttcccgc cggtttttac gctgaggtga
     3541 taggaggcca tcgtgttttt atcccgctga aggcgcgcac cgtttctgaa cgaagtgaag
     3601 aaacgtctaa gtgcgccctg ataaataaaa gagttatcag ggattgtagt tgaatttgac
     3661 ctccactgcc atcaatgagc ataattcccc cgttaaaact cagagggtaa acgggcatga
     3721 gtaaaagcga aaaacaggaa caatgggcag cagaaagagt gcagtatatt cgcggcttaa
     3781 agtcgccgaa tgagcaacag aaacttatgc tgatactgac ggataaagca gataaaacag
     3841 cacaggatat caaaacgctg tccctgctga tgaaggctga acaggcagca gagaaagcgc
     3901 aggaagccag agcgaaagtc atgaacctga tacaggcaga aaagcgagcc gaagccagag
     3961 ccgcccgtaa agcccgtgac catgctctgt accagtctgc cggattgctt atcctggcgg
     4021 gtctggttga cagtaagacg ggtaagcctg ttgatgatac cgctgcctta ctgggtgcat
     4081 tagccagtct gaatgacctg tcacgggata atccgaagtg gtcagactgg aaaatcagag
     4141 ggcaggaact gctgaacagc aaaaagtcag atagcaccac atagcagacc cgccataaaa
     4201 cgccctgaga agcccgtgac gggcttttct tgtattatgg gtagtttcct tgcatgaatc
     4261 cataaaaggc gcctgtagtg ccatttcccc cattcactgc cagagccgtg agcgcagcga
     4321 actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac aaaaggaata
     4381 ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt ttaaggtctg
     4441 ttttgtagag gagcaaacag cgtttgcgac atccttttta atactgcgga actgactaaa
     4501 gtagtgagtt atacacaggg ctgggattta ttctttttat ctttttttat tctttcttta
     4561 ttctataaat tataaccact tgaatataaa caaaaaaaac acacaaaggt ctagcggaat
     4621 ttacagaggg tctagcagaa tttacaagtt ttccagcaaa ggtctagcag aatttacaga
     4681 tacccacaac tcaaaggaaa aggactagta attatcattg actagcccat ctcaattggt
     4741 atagtgatta aaatcaccta gaccaattga gatgtatgtc tgaattagtt gttttcaaag
     4801 caaatgaact agcgattagt cgctatgact taacggagca tgaaaccaag ctaattttat
     4861 gctgtgtggc actactcaac cccacgattg aaaaccctac aaggaaagaa cggacggtat
     4921 cgttcactta taaccaatac gctcagatga tgaacatcag tagggaaaat gcttatggtg
     4981 tattagctaa agcaaccaga gagctgatga cgagaactgt ggaaatcagg aatcctttgg
     5041 ttaaaggctt tgagattttc cagtggacaa actatgccaa gttctcaagc gaaaaattag
     5101 aattagtttt tagtgaagag atattgcctt atcttttcca gttaaaaaaa ttcataaaat
     5161 ataatctgga acatgttaag tcttttgaaa acaaatactc tatgaggatt tatgagtggt
     5221 tattaaaaga actaacacaa aagaaaactc acaaggcaaa tatagagatt agccttgatg
     5281 aatttaagtt catgttaatg cttgaaaata actaccatga gtttaaaagg cttaaccaat
     5341 gggttttgaa accaataagt aaagatttaa acacttacag caatatgaaa ttggtggttg
     5401 ataagcgagg ccgcccgact gatacgttga ttttccaagt tgaactagat agacaaatgg
     5461 atctcgtaac cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa ataccaacaa
     5521 ccattacatc agattcccac ctacataacg gactaagaaa aacactacac gatgctttaa
     5581 ctgcaaaaat tcagctcacc agttttgagg caaaattttt gagtgacatg caaagtaagt
     5641 atgatctcaa tggttcgttc tcatggctca cgcaaaaaca acgaaccaca ctagagaaca
     5701 tactggctaa atacggaagg atctgaggtt cttatggctc ttgtatctat cagtgaagca
     5761 tcaagactaa caaacaaaag tagaacaact gttcaccgtt acatatcaaa gggaaaactg
     5821 tccatatgca cagatgaaaa cggtgtaaaa aagatagata catcagagct tttacgagtt
     5881 tttggtgcat tcaaagctgt tcaccatgaa cagatcgaca atgtaacaga tgaacagcat
     5941 gtaacaccta atagaacagg tgaaaccagt aaaacaaagc aactagaaca tgaaattgaa
     6001 cacctgagac aacttgttac agctcaacag tcacacatag acagcctgaa acaggcgatg
     6061 ctgcttatcg aatcaaagct gccgacaaca cgggagccag tgacgcctcc cgtggggaaa
     6121 aaatcatggc aattctggaa gaaatagcgc tttcagccgg caaaccggct gaagccggat
     6181 ctgcgattct gataacaaac tagcaacacc agaacagccc gtttgcgggc agcaaaaccc
     6241 gtacttttgg acgttccggc gggtttttgt ggcgagtggt gttcgggcgg tgcgcgcaag
     6301 atcgcttatg ttaaatacag cagaaaaacc ataaaattac cctataacgc aaattgcttt
     6361 ttatgctaat atttcgcaat ggagggcgaa ccagtgttta acgtcatatt gcacgatgat
     6421 gtaccaaatg agctggctgg cttaccgcca gtagtcagag cgaagatgat tcgcctgatt
     6481 gataaactta aaaccaacgc tactgcatta cgagagccag atagcaaacc actccgggat
     6541 ggattatttg agttaaggac gatggggacg gatattgcca gaggtcttta tgtttatcag
     6601 aaaggcagga atatctatct acttagagtc tttattaaaa agactcagaa aaccccatcg
     6661 ggtgaaatat ctatggcctt tgaacgtctt aaggagatgc taaatgagta aagttaaagg
     6721 tatcgcttgg gagcatgtta ggaatgaatt gatgtctaat cctgaggttc aagcagccta
     6781 tgaagcagag gagcgcaagg aaagacttca agctatgctc gctgaatggc gtcatcacgc
     6841 aggattaaca agagcccagg tggcagaacg catgggtgtt actccaccta ccgtttctcg
     6901 catggaatct aatgtgatta aggctagcat tgaaacactg gctcgatacg caagagcatg
     6961 cggtgttaag caccctcaga tcacgttgta attttcaagg ccgctttaag cagccttttg
     7021 atgcgtgcaa gatcgcttat gttgaacaag gagttttttt gcaaaaaacc atcatttcac
     7081 acatatgaga taatcctaag ggatataaat aggctaattt tgcccaacgc gccaaaaatg
     7141 taaagttagc atttacagct tgtaagccat tgttctagtt tcacaacggc aaattgatgt
     7201 taagtctaaa agaccgtgat aactcattgt ggaagattga gagctaaatg taacaataga
     7261 ggacattgtt agtccaagat ttaaggggtg aatatgtcac ccctaagata aaacatttac
     7321 agccatatct aatacctgca aggtgcaaaa atatcgagtt ggctctgata gacggtggtt
     7381 tttacattca taagtcctag caaactgtcg aacaaattgt cgtgggaaaa gccgccctgt
     7441 tctgctcgct gtgcaacaca agtcatgttc aactgattat cttggctaaa gtcattagat
     7501 acccaagcca gcattggtac gctagtttgt tctttcggtg caatactata gggcgcacca
     7561 tgtaaataca tgcccttttc acccaaagac tcaccatggt cagagagata cagcattgca
     7621 gtatcgaaca tatcctgctt accttttaat ttattcacca cttcactgag aataaaatcc
     7681 gtatatagaa tagtgttgtc gtaggtgtta atcagttctt cttgactgca attttgaata
     7741 tcactgcgcg gacaatccgg tataaattta cgatgctctg gcgggtatct aagataataa
     7801 gttggtccat gactaccaat gatatgcaaa aaaattactg tatcttgact tggtgctact
     7861 gccagaattt tatctaattt gttgagcaat acttggtcaa aacaatattg gccagaacac
     7921 agcttcggat cactcttcaa atctatcgtg agattttcaa cctgatcaca cacaccttta
     7981 cagccagaat cattatcaaa ccactgtact tttataccac tatgacttaa cacatcaatc
     8041 actgtgtctt gagcattagg gaaggtgcga acaagttcct gatatgagat catcatattc
     8101 atccggagcg catcccaaag ggacatcatg agccatcaac tcaccttcgc cgatagtgaa
     8161 ttcagcacta agcgccgtca gacccgaaaa gagattttcc tctcccgcat ggagcagatt
     8221 ctgccatggc agaatatgac cgctgtcatc gagccgtttt atcccaaggc gggcaatggc
     8281 cgacggccct atccgctgga gaccatgctg cgtattcact gcatgcagca ttggtacaac
     8341 ctgagcgacg gtgccatgga agatgccctg tacgaaatcg cctccatgcg cctgtttgcc
     8401 cgattatccc tggatagcgc cctgccggat cgcaccacta tcatgaattt ccgccacctg
     8461 ctcgagcagc atcaactggc ccgtcaattg ttcaagacca tcaatcgctg gctggccgaa
     8521 gcaggcgtca tgatgaccca aggcactttg gtggatgcca ccatcattga ggcacccagc
     8581 tctaccaaga acaaagagca gcaacgcgat ccggagatgc atcagaccaa gaaaggcaat
     8641 cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg ccaagagtgg cctgacccac
     8701 agcctggtca ccaccgcggc caacgagcat gacctcaatc agctgggtaa tctgcttcat
     8761 ggagaggagc aatttgtctc agccgatgcc ggctaccaag gagcgccaca gcgcgaggag
     8821 ctggccgagg tggatgtgga ctggctgatc gccgagcgtc ccggcaaggt aaaaaccttg
     8881 aagcagaatc cgcgcaagaa caaaacggcc atcaacatcg aatacatgaa agccagcatc
     8941 cgtgccaggg tggagcaccc gtttcgcatc atcaagcggc agttcggctt cgtgaaagcc
     9001 agatacaagg ggctgctgaa aaacgataac caactggcga tgttattcac cctggccaac
     9061 ctgtttcggg tggaccaaat gatacgtcag tgggagagat ctcagtaaaa accggaaata
     9121 acgccagaaa tggtggaaaa aatagcctaa ataggctgat tcgatgtgtt tgcgggaaaa
     9181 aaatcggccc agatccgcga aattttaatc agcgagtcaa cttgggaaga aatgacctgc
     9241 ttattcgcac cttccctaaa gctcgaagct gtataaccat ctgacctgca attcgttgat
     9301 gataaagggt tagaccccgt gaggcctatc tggctattgt acaggatata cgtatccgct
     9361 taacgaacaa gaaccacggc agcctggcgg ctgcaaatcg tggtgtggca gaatatgagc
     9421 tttcagagta accatccaga atgagttccc
//
