LOCUS       CP122750                6213 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC1745 plasmid unnamed10, complete
            sequence.
ACCESSION   CP122750
VERSION     CP122750.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549622
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 272x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 12:23:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,733
            CDSs (total)                      :: 5,605
            Genes (coding)                    :: 4,395
            CDSs (with protein)               :: 4,395
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 1,210
            CDSs (without protein)            :: 1,210
            Pseudo Genes (ambiguous residues) :: 0 of 1,210
            Pseudo Genes (frameshifted)       :: 1,054 of 1,210
            Pseudo Genes (incomplete)         :: 160 of 1,210
            Pseudo Genes (internal stop)      :: 64 of 1,210
            Pseudo Genes (multiple problems)  :: 63 of 1,210
            Pseudo Genes (short protein)      :: 4 of 1,210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1745"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed10"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            join(6190..6213,1..249)
                     /locus_tag="QDY25_28620"
                     /pseudo=""
     CDS             join(6190..6213,1..249)
                     /locus_tag="QDY25_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            complement(567..800)
                     /locus_tag="QDY25_28625"
     CDS             complement(567..800)
                     /locus_tag="QDY25_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WHG96865.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(807..1325)
                     /locus_tag="QDY25_28630"
     CDS             complement(807..1325)
                     /locus_tag="QDY25_28630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WHG96866.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(1406..2014)
                     /locus_tag="QDY25_28635"
     CDS             complement(1406..2014)
                     /locus_tag="QDY25_28635"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WHG96867.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLE
                     LNFVIPNMELQSGKRLQPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRILTLPDNL
                     PETKQALAESDHTRDRRSLSCRGDKRPSGCDSGAHRGRA"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2004..2328)
                     /locus_tag="QDY25_28640"
                     /pseudo=""
     CDS             complement(2004..2328)
                     /locus_tag="QDY25_28640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2398..2589
                     /locus_tag="QDY25_28645"
     CDS             2398..2589
                     /locus_tag="QDY25_28645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WHG96868.1"
                     /translation="MTKQEKTAINMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3704..3976
                     /locus_tag="QDY25_28650"
     CDS             3704..3976
                     /locus_tag="QDY25_28650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG96862.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSCT
                     VCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
                     /name="hypothetical protein"
     gene            3957..5166
                     /locus_tag="QDY25_28655"
     CDS             join(3957..4796,4798..5166)
                     /locus_tag="QDY25_28655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0004803 - transposase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WHG96863.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLA
                     CGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVH
                     LVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSHITTE
                     SQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFR
                     YLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGM
                     DKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNARDISLLRY
                     MPA"
                     /name="IS91 family transposase"
     gene            complement(5335..5454)
                     /gene="tnpB"
                     /locus_tag="QDY25_28660"
                     /pseudo=""
     CDS             complement(5335..5454)
                     /gene="tnpB"
                     /locus_tag="QDY25_28660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /name="tnpB"
     gene            complement(5454..6104)
                     /gene="tnpA"
                     /locus_tag="QDY25_28665"
     CDS             complement(5454..6104)
                     /gene="tnpA"
                     /locus_tag="QDY25_28665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0004803 - transposase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="WHG96864.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIFS
                     LVRRFHQVFTDWPLSGEYSCGQLARALFPGRYPSAPTVTQPVKAEKPRRNRFSPEFKWR
                     LVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPEPDN
                     KIPTPVQIPEQINTLPDSLCCELVLPAGTLRLKGELTPALLQTLIREMKGSSH"
                     /name="tnpA"
     Typing          complement(2677..2757)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.341"
                     /coverage="95.0"
                     /evalue="5.11e-30"
     Typing          2768..3328
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.428"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(464..1506)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="86.761"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1605..2014)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.07e-86"
     Typing          complement(2048..2358)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.269"
                     /coverage="88.0"
                     /evalue="2.42e-57"
ORIGIN
        1 atgatggtgc ctttttttcg gcagttctgg gatatgggag ctaaagataa agcgacagga
       61 aaatcacgga gtgatgtgca acaacgcatt cagcagtttc acagccagga atttttaaac
      121 tcactccggg gcacaactca atttgcgggt actgattacc gcagcaaaga ccttaccccg
      181 aaaaaatcca ggctgctggc tgacacgatt tctgcggttt atctcgatgg ctacgagggc
      241 agacagtaag tggatttacc ataatccctt aattacgcgc accgctaaaa cgcgttcagc
      301 gcgatcacgg cagcagacag gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc
      361 gcgcctgata aattttaacc gcatgaatac ctatgcaacc agatggtaca gaccacatta
      421 gcccgtctta atccacttaa cctgccattt ttcatagttt caccatccca gtgaaggacc
      481 atccagtgtg cgttcctgta tttccggctg gcgttcccgc tccagggata actcatgttc
      541 gcgttcctgt atcagccgtt cgtcccttat ctcctgttct cgctgtataa ctggctcaag
      601 cgttctgtcc gctcgctcaa gtgctgcacc tgctgactca actgcatgac ctgctcgttc
      661 agcatcgcgt tgtcccgttg cgtaagcgaa aacattttct gcaattccac gaaggcgctc
      721 tcccattcgt tcagccgctg catatagtcc tgttgcagtt gctctaatgc gttcagcaaa
      781 tgtctttcca gctccgtcac tctgtgtcac tccgtcagtt gtacccagtc cttcccctga
      841 tagggaatca ccgttgccga tttcccctgc gggatgacca gaaatttcct tctcccgtcc
      901 tgcacaaact ccacgcccca tgtcttcgcg ttcagtttct gcaatgtctc ttcctgcttc
      961 ctgatttctt ccaggttcgt ctgtatccgc ccgccaagat accagagcgt cccgccactc
     1021 acggtaaaca ggaaaatgac catccccagt aacatcatgc ccgtattccc tgccagcttt
     1081 aacacgtcct tcctgtgctg catcatcgcc tctttcaccc cttcccggtg ttttttcagt
     1141 gattcctctg tcgaagctgt gaacagggct atagcgtctt tgattttcgt ctcgtttgat
     1201 gtcacagcct tgcttacaga tttttcgagc ttgctgaact cgtcgttcag cattgtctct
     1261 gtagattcgg ctctctcttt cagctttttc tcgaactccg cgcccgtctg taaaagattg
     1321 ctcataaaat gctcctttca gcctgatatt cttcccgccg ttcgggtctg caatgctgat
     1381 actgcttcgc gtcaccctga ccacttcaag ccctgcctct gtgagcgcct gaatcacatc
     1441 ctgacggcct tttatctccc cgacatgata aagagcgtct atcccgcgtg tgatcgctct
     1501 ctgcaagcgc ctgtttcgtt tccggcaggt tatcagggag agtcagtatc cggcggttct
     1561 ccggggcgtt ggggtcatgc agcccgtaat ggtgattaac cagcgtctgc caggcatcaa
     1621 ttctcggcct gtctgcccgg tcgtagtacg gctggaggcg ttttccgctc tgtagctcca
     1681 tgttcggaat gacaaaattc agctcaagcc gtcccttgtc ctggtgctcc acccacagga
     1741 tgctgtactg atttttttcg agaccgggca tcagtacacg ctcaaagctc gccatcacct
     1801 tttcacgtcc tcccggcggc agctccttct ccgcgaacga cagaacacct gacgtgtatt
     1861 tcttcgcaaa tggcgtggca tcgatgagtt cccggacttc ttccggatta ccctgaagca
     1921 ccgttgcccc ttcccggtta cgctcccttc ccagcaggta atcaaccgga ccactgccac
     1981 cgccttttcc cctggcatga aacttaacta tcatcccgcg ctccatgttc cctgacgacc
     2041 tgccgtaagc tggcgcaact ctctctcgat ggccatcagt gcggccacca cctgaacccg
     2101 gtcaccggaa gaccactgcc cgctgttcac cttcctcgcc gtctgattca gattattccc
     2161 gatggcagcc agctggcgca gtaatggcgg cgatagtgtc ggcagcttcc cggaacgggc
     2221 aaccggctca cccagacaga cccgccgcat ccatactgcc agctgtttac cctcacagcg
     2281 ttcaagtaac cgggcatgtt catcatcagt aacccgtatt gtgagcatct tctcgcgttt
     2341 catcggtatc attaccccat aaaacagaaa tcccccatta cacggaggca tcagaccatg
     2401 actaaacagg aaaaaaccgc cattaacatg gcccgcttta tcagaagcca gacgttaacg
     2461 ctgctggaga aactcaacga actggacgcg gatgaacagg ccgatatttg tgaatcactt
     2521 cacgaccacg ccgacgagct ttaccgcagc tgcctcgcac gcttcggcga tgacggtgaa
     2581 aacctctgac acatacagct cccggagacg ttcacagctt gtctgtgagc ggatgccggg
     2641 agcagacaag cccgtcaggg cgcgtcagcg ggtttggtgt cggggcgcag ccctgaacca
     2701 gtcacgtagc gctagcggag tgtatactgg cttaactatg cggcatcagt gcagattata
     2761 tgaaaagtgc accatatgcc gggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2821 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct acgctcggtc gttcggctgc
     2881 ggcgagcggt atcagctcac tcaaagacgg taatgcggtt atccacagaa tcaggggata
     2941 acgccaaaag aaacatgtga gcaaaaaaca agaacccgga agaaggccac gccgctggcg
     3001 tttttccata ggctccgccc ccctgacgag catcacagaa atcgatgctc aagtcagagg
     3061 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     3121 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     3181 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gctcggtgta ggccgttcgc
     3241 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3301 aactatcgtc ttgagtccaa cccggtaaga cacgacttaa cgccactgtc agcagccatt
     3361 ggtagagggt gtacaagaaa ttgctgatac ggagttcttg aagtgaaggc ccgactgcgg
     3421 ctacactgaa aggacagatt tggtggcagc ggtcttgtac gccctgttac cttgggttag
     3481 aaagcctgag aaacttctaa ccttcgaaaa acccacgcct gagaacgtgg gttttttcgt
     3541 ttacgcccgt agagattacc catcataggg gtgtaactca aggtctattt ggatagtttt
     3601 accaaggaat aatcatggag ttcgaaccac ggcagcctgg cggctgcaaa tcgtggtgtg
     3661 gcagaatatg agctttcaga gtaaccatcc agaatgagtt cccatgtcac acaacctgga
     3721 gcaccagaaa gtccacacca gaatggtgaa agaggttctg aaagccgtcg ccagggccaa
     3781 taatcaccct tatcagtctg tatttacaga ctttatcgcc ggtcatccct cctgtacggt
     3841 ctgtttctgg gaaaccttcc acaaaatgta cccggattca ccgtacgaat acgtcacgtt
     3901 ctgccatacc tgccgtcgtt ttgatttata cgaaactgaa gcggagatga aggcggatga
     3961 cccgaagtgg tggtgatttt cagccccgac cgctgaaacg cctgttcaca gccaaccagt
     4021 gctggacatc cttcctggat gcgggcggcc tgcgcgatat cgaggttgag gctgtcacaa
     4081 aaatgctggc ctgcggcacg cggatactgg gtgtgaagga atacaactgc gataaacctg
     4141 agtgccccca cgtcagatac gtcactaact catgtggcag ccgtgcctgc ccgtcctgcg
     4201 gaaaaaaggc cacagacctg tggatagcga cacagctgaa tcgtcttcct gactgcgact
     4261 gggtgcacct ggtcttcacc ctgccggaca cgctgtggcc ggtgttcgaa agcaaccgct
     4321 ggctgctgaa tgacgtgtgc cgtctggcgg tggagaatct gctgtatgca gcccggaaac
     4381 ggggtctgga acccggtatc ttctgcgcca tccatacgta tggccgtcgt ctcaactggc
     4441 atccgcatgt gcatgtgtca gtgacctgcg gaggtctgaa taaacacgga cactggaaaa
     4501 agctgagctt cctgaaagac gcgatgcgtt cgcggtggat gtggaatatg cggcagctgc
     4561 ttctgaaagc gtggtcagaa ggtctggcga tgccggaatc gttgtcacat atcacgacgg
     4621 aatcacagtg gagaagcctg gtgctgaaag ccggcggaaa atactggcat gtgtacatgt
     4681 cgaagaagac agccggaggg cggaatacgg cacgctacct gggtcgttat ctgaagaagc
     4741 caccaatagc ggcttcccgg ctggcgcatt acaacggagg agcgagtctg agcttcccgt
     4801 tacctggacc acaaaacggg agaaacggcg acggaaacgc tgacacagcg ggagctggtc
     4861 gcgaggctga aacagcacat cccggagaag ttttttaaga tggtgagata cttcgggttc
     4921 cttgccaacc gggtgtgtgg agagaagctg ccgcaggtgt accgtgctct ggggatggat
     4981 aagccggaac cagtggcgaa agtgtgctat gcacaaatgg tgaaacagtt cctgagtcgt
     5041 gacccgttcg aatgcgtgct gtgtggcggc cggatggtat accgccgggc cattgcgggg
     5101 ctgaatgtat cagggctgaa gaaaaacgcg cgggatatca gtctgctgag gtatatgccg
     5161 gcctgacaca ggctaagtgt gcccgggaat cggtgcaggt ataaaaaaca gcctgttacg
     5221 gtataaaaat cgaccatgca gagagagaaa ccaccggagt ggagggaaaa gcagaaaggc
     5281 tcacgtggca taaggcgtgg acagcggtat cgtctggttt ttcaatttcc tatacgtgaa
     5341 cgggtcatcc ttcaggacgt tctgaacttt tgaggccagg ccgttaaagc catttcgcat
     5401 atcggtgata ccggcaacca gccagatacg cgaaccggca gggagagata tcatcagtgg
     5461 ctgctccctt tcatttcgcg gataagtgtc tgtaataacg ccggtgtcag ttcacctttc
     5521 agcctgagag ttccggccgg cagaaccagc tcgcagcaca gactgtctgg cagtgtattt
     5581 atctgttcag gtatctgcac tggcgtcggg attttattat ccggctccgg agttaacgtc
     5641 acgggaagta gcgatgtttc atgttcgccg ggcggttgca ggccaccgtt acgccagaga
     5701 tgccgccagt taaagagcag gttatcgtta attccattct ctcgagctat ttgtgcgaca
     5761 caagcaccgg gtaaaagagt ttgctgaacc agtctccatt taaactccgg tgaaaatcgg
     5821 ttccggcggg gtttctctgc tttcacaggc tgagtcacgg tcggggctga agggtatcgc
     5881 cccgggaaaa gagcccgggc cagttgcccg caggaatatt caccggaaag aggccagtca
     5941 gtgaatacct ggtgaaatcg cctcaccaga gaaaaaatgg cggttttgct gacaccggtc
     6001 tgcgcagaaa tgcgcgggta cgacagtgac tcgtcaaatc tcagctgtag cacacgcagg
     6061 atcaattccg gattgtctgc cagtaccgct tttcgtgcat tcatcttaaa tgtcccttta
     6121 ctgcgaaaat ggacattagt atcggaaaca ggaaagggag gcgaaagacg gtttaaatga
     6181 gacggttaca cccaaaaagc cgacacaaca cga
//
