LOCUS       CP122743                7741 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC1745 plasmid unnamed3, complete
            sequence.
ACCESSION   CP122743
VERSION     CP122743.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549622
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 272x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 12:23:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,733
            CDSs (total)                      :: 5,605
            Genes (coding)                    :: 4,395
            CDSs (with protein)               :: 4,395
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 1,210
            CDSs (without protein)            :: 1,210
            Pseudo Genes (ambiguous residues) :: 0 of 1,210
            Pseudo Genes (frameshifted)       :: 1,054 of 1,210
            Pseudo Genes (incomplete)         :: 160 of 1,210
            Pseudo Genes (internal stop)      :: 64 of 1,210
            Pseudo Genes (multiple problems)  :: 63 of 1,210
            Pseudo Genes (short protein)      :: 4 of 1,210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1745"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            join(7252..7741,1..492)
                     /locus_tag="QDY25_26760"
     CDS             join(7252..7698,7700..7741,1..492)
                     /locus_tag="QDY25_26760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048909999.1"
                     /ribosomal_slippage=""
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology. GO_function: GO:0004803 - transposase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="WHG96557.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPFY
                     PKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDRTT
                     IMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQRDPE
                     MHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVSADAG
                     YQGAPQREELAEVDVDWLIAERPGKVKTLKQNPRKNKTAINIEYMKASIRARVEHPFRI
                     IKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
                     /name="IS5-like element ISKpn26 family transposase"
     gene            complement(977..1561)
                     /locus_tag="QDY25_26765"
     CDS             complement(977..1561)
                     /locus_tag="QDY25_26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WHG96558.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(1574..2671)
                     /gene="mobA"
                     /locus_tag="QDY25_26770"
     CDS             complement(1574..2671)
                     /gene="mobA"
                     /locus_tag="QDY25_26770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WHG96559.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     DTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQR
                     QQEKEQQRLAEQTRQKPDKGWSFSR"
                     /name="mobA"
     gene            2837..3304
                     /gene="traD"
                     /locus_tag="QDY25_26775"
     CDS             2837..3304
                     /gene="traD"
                     /locus_tag="QDY25_26775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WHG96560.1"
                     /translation="MSKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     gene            3900..4850
                     /locus_tag="QDY25_26780"
     CDS             3900..4850
                     /locus_tag="QDY25_26780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003887 - DNA-directed DNA polymerase activity [Evidence
                     IEA]; GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WHG96553.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            4858..5271
                     /locus_tag="QDY25_26785"
     CDS             4858..5271
                     /locus_tag="QDY25_26785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG96554.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            5518..5835
                     /locus_tag="QDY25_26790"
     CDS             5518..5835
                     /locus_tag="QDY25_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WHG96555.1"
                     /translation="MFNVILHDDVPNELAGLPPVVRAKMIRLIDKLKTNATALREPDSK
                     PLRDGLFELRTMGTDIARGLYVYQKGRNIYLLRVFIKKTQKTPSGEISMAFERLKEMLN
                     E"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5828..6115
                     /locus_tag="QDY25_26795"
     CDS             5828..6115
                     /locus_tag="QDY25_26795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WHG96556.1"
                     /translation="MSKVKGIVWEHVRNELMSNPEVQAAYEAEERKERLQAMLAEWRHH
                     AGLTRAQVAERMGVTPPTVSRMESNVIKASIETLARYARACGVKHPQITL"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(6453..7178)
                     /locus_tag="QDY25_26800"
                     /pseudo=""
     CDS             complement(6453..7178)
                     /locus_tag="QDY25_26800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011638903.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase activity
                     [Evidence IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /name="sulfatase-like hydrolase/transferase"
     ARG             complement(6454..7220)
                     /name="MCR-4.6"
                     /gene="MCR-4.6"
                     /gene_name="MCR phosphoethanolamine transferase"
                     /tool="rgi"
                     /drug_class="peptide antibiotic"
                     /identity="100.0"
                     /coverage="47.13"
     Typing          4675..4850
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(1578..2671)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcatcaga ccaagaaagg caatcagtgg cactttggca tgaaggccca cattggtgtc
       61 gatgccaaga gtggcctgac ccacagcctg gtcaccaccg cggccaacga gcatgacctc
      121 aatcagctgg gtaatctgct tcatggagag gagcaatttg tctcagccga tgccggctac
      181 caaggagcgc cacagcgcga ggagctggcc gaggtggatg tggactggct gatcgccgag
      241 cgtcccggca aggtaaaaac cttgaagcag aatccgcgca agaacaaaac ggccatcaac
      301 atcgaataca tgaaagccag catccgtgcc agggtggagc acccgtttcg catcatcaag
      361 cggcagttcg gcttcgtgaa agccagatac aaggggctgc tgaaaaacga taaccaactg
      421 gcgatgttat tcaccctggc caacctgttt cgggtggacc aaatgatacg tcagtgggag
      481 agatctcagt aaaaaccgga aataacgcca gaaatggtgg aaaaaatagc ctaaataggc
      541 tgattcgatg tgtttgcggg aaaaaaatcg gcccagatcc gcgaaatttt aatcagcgag
      601 tcaacttggg aagaaatgac ctgcttattc gcaccttccc taaagctcga agctgtataa
      661 ccatctgacc tgcaattcgt tgatgataaa gggttagacc ccgtgaggcc tatctggcta
      721 ttgtacagga tatacgtatc cgcttaacga acaagaacct cacgcgcgtc tttcatcagg
      781 gcccgaatcg atgacgattc atcatggccg tttatagtac cgcagtctct cccctttatt
      841 tttactgaag agcagacaga aaccataaat accggctttg acatgccggg gaagtcctta
      901 tagtacaata attctctata tccaaactga ccccctaatt cacgacaaca ataacggcgc
      961 aatgtgcgcc gttatgttat tttttgataa tcatccaggt attctcttta tcaccatacg
     1021 tagctcgggt gtccacctta acgcagggct ttccgtcaca acgcgatata tcagccagcg
     1081 gggctttctt ctgccagacc gcttccatcc gttgcatttc agcaatctgg ctgtacccgt
     1141 cattcataaa ccacgtaaat gccgtcacgc aggcagccat gacgaaaaat atcgtcagca
     1201 cgagataaat cgcggatttc cacgtatagc gcgacatctc gcgtcgcatt tcatggatca
     1261 tcgccttcgc cgtatcggta gcctgattaa gcgcttctgt cgccggtttc tgctgtgcta
     1321 atccggcttg tttcagctct ttctcaacct gaatgagcgc ggcatgaaca gattgcctga
     1381 cggtttcagt catattaccg gacgcgctgt ccagctcacg aatgagcctg ctcagcgtgt
     1441 cactctgctg ctgtaattgt gctgaggcgt cctgaaactg ctcagtcaga gaggtaacac
     1501 gcttttccag cgcctgatga tgcccgatga gggcagcgat ttgtttaatt tcgtcgctca
     1561 tgaaaaatcc tgtctatcgt gaaaatgacc agcctttatc cggcttctgt cgtgtctgtt
     1621 cggcgagtct ctgctgttct ttctcctgct gacgctgttt ttctgccagt cgctccattt
     1681 cctgatgtat cccctgaaac tccgccattg tctcgttcac aagggactga aggtcgattt
     1741 cgtcctgtac gtccttcagc gcatcactga ccagtgcgtt cagcttctcc ggctctttct
     1801 caaaatcgaa aaccctgccg gaagtgtctt cctgcgcagg ttcaggcttc tgctcttgtt
     1861 ttatcgtcag gttatcccgc tcgctgatgg cttcccgtag cgaggtggtc acgtcaatca
     1921 cactgtcacg ttcatcacga gactgctccg cctgcctttc agcgtccctg cgctcaagga
     1981 tggcctgtaa ctgcgcagta tcgaaccgct gaacctgtcc cgcgccaaga tgccgctcag
     2041 gctcacgctc aatgccctga gctttgagtg agcgtgcatc aacgcggtcg gtatgctgat
     2101 accgttcaag gtgcttattc tgtaggtcag cccagcgttc ccgctgggcg acgaggtgct
     2161 ctttgcgttc tgtcggcgtt tccccgaaac gtgccttttt cgcaccgccg cgctcagggg
     2221 ctttgctgtt agctcgctta aaatactgct gcgggtcacg gtcgataccg tcattcatcc
     2281 gctcagagaa catgatatgc gcgtgcggct gctcgccgcc ggctatcgcc gctttcggat
     2341 tatgaatggc gaactgatac gcatgacggt cgccgatttc ctgccggaca aaatcgcgga
     2401 ccagttcaag gcgttgttcg ggtttcagct cacgcggcag ggcaatctcg atttcacggt
     2461 aggtacagcc gttggcacgt tcaaacgtgt cagcggcttt ccagaactcc gtcggtttat
     2521 gcgctgccca ggcaggcata ttgccggact ccttgtgctc aaggtcggag tctttttcac
     2581 gggcatactt tccctcacgc gaaatataat cggcatgggg agaggcactg cctttcccgc
     2641 cggtttttac gctgaggtga taggaggcca tcgtgttttt atcccgctga aggcgcgcac
     2701 cgtttctgaa cgaagtgaag aaacgtctaa gtgcgccctg ataaataaaa gagttatcag
     2761 ggattgtagt tgaatttgac ctccactgcc atcaatgagc ataattcccc cgttaaaact
     2821 cagagggtaa acgggcatga gtaaaagcga aaaacaggaa caatgggcag cagaaagagt
     2881 gcagtatatt cgcggcttaa agtcgccgaa tgagcaacag aaacttatgc tgatactgac
     2941 ggataaagca gataaaacag cacaggatat caaaacgctg tccctgctga tgaaggctga
     3001 acaggcagca gagaaagcgc aggaagccag agcgaaagtc atgaacctga tacaggcaga
     3061 aaagcgagcc gaagccagag ccgcccgtaa agcccgtgac catgctctgt accagtctgc
     3121 cggattgctt atcctggcgg gtctggttga cagtaagacg ggtaagcctg ttgatgatac
     3181 cgctgcctta ctgggtgcat tagccagtct gaatgacctg tcacgggata atccgaagtg
     3241 gtcagactgg aaaatcagag ggcaggaact gctgaacagc aaaaagtcag atagcaccac
     3301 atagcagacc cgccataaaa cgccctgaga agcccgtgac gggcttttct tgtattatgg
     3361 gtagtttcct tgcatgaatc cataaaaggc tgcctgtagt gccatttccc cccattcact
     3421 gccagagccg tgagcgcagc gaactgaatg tcacgaaaaa gacagcgact catagtgcct
     3481 gatggtcgga gacaaaagga atattcagcg atttgcccga gcttgcgagg gtgctactta
     3541 agcctttagg gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc gacatccttt
     3601 ttaatactgc ggaactgact aaagtagtga gttatacaca gggctgggat ctcattcttt
     3661 ttatcttttt ttattctttc tttattctat aaattataac cacttgaata taaacaaaaa
     3721 aaacacacaa aggtctagcg gaatttacag agggtctagc agaatttaca agttttccag
     3781 caaaggtcta gcagaattta cagataccca caactcaaag gaaaaggact agtaattatc
     3841 attgactagc ccatctcaat tggtatagtg attaaaatca cctagaccaa ttgagatgta
     3901 tgtctgaatt agttgttttc aaagcaaatg aactagcgat tagtcgctat gacttaacgg
     3961 agcatgaaac caagctaatt ttatgctgtg tggcactact caaccccacg attgaaaacc
     4021 ctacaaggaa agaacggacg gtatcgttca cttataacca atacgctcag atgatgaaca
     4081 tcagtaggga aaatgcttat ggtgtattag ctaaagcaac cagagagctg atgacgagaa
     4141 ctgtggaaat caggaatcct ttggttaaag gctttgagat tttccagtgg acaaactatg
     4201 ccaagttctc aagcgaaaaa ttagaattag tttttagtga agagatattg ccttatcttt
     4261 tccagttaaa aaaattcata aaatataatc tggaacatgt taagtctttt gaaaacaaat
     4321 actctatgag gatttatgag tggttattaa aagaactaac acaaaagaaa actcacaagg
     4381 caaatataga gattagcctt gatgaattta agttcatgtt aatgcttgaa aataactacc
     4441 atgagtttaa aaggcttaac caatgggttt tgaaaccaat aagtaaagat ttaaacactt
     4501 acagcaatat gaaattggtg gttgataagc gaggccgccc gactgatacg ttgattttcc
     4561 aagttgaact agatagacaa atggatctcg taaccgaact tgagaacaac cagataaaaa
     4621 tgaatggtga caaaatacca acaaccatta catcagattc ccacctacat aacggactaa
     4681 gaaaaacact acacgatgct ttaactgcaa aaattcagct caccagtttt gaggcaaaat
     4741 ttttgagtga catgcaaagt aagtatgatc tcaatggttc gttctcatgg ctcacgcaaa
     4801 aacaacgaac cacactagag aacatactgg ctaaatacgg aaggatctga ggttcttatg
     4861 gctcttgtat ctatcagtga agcatcaaga ctaacaaaca aaagtagaac aactgttcac
     4921 cgttacatat caaagggaaa actgtccata tgcacagatg aaaacggtgt aaaaaagata
     4981 gatacatcag agcttttacg agtttttggt gcattcaaag ctgttcacca tgaacagatc
     5041 gacaatgtaa cagatgaaca gcatgtaaca cctaatagaa caggtgaaac cagtaaaaca
     5101 aagcaactag aacatgaaat tgaacacctg agacaacttg ttacagctca acagtcacac
     5161 atagacagcc tgaaacaggc gatgctgctt atcgaatcaa agctgccgac aacacgggag
     5221 ccagtgacgc ctcccgtggg gaaaaaatca tggcaattct ggaagaaata gcgctttcag
     5281 ccggcaaacc ggctgaagcc ggatctgcga ttctgataac aaactagcaa caccagaaca
     5341 gcccgtttgc gggcagcaaa acccgtactt ttggacgttc cggcgggttt ttgtggcgag
     5401 tggtgttcgg gcggtgcgcg caagatcgct tatgttaaat acagcagaaa aaccataaaa
     5461 ttaccctata acgcaaattg ctttttatgc taatatttcg caatggaggg cgaaccagtg
     5521 tttaacgtca tattgcacga tgatgtacca aatgagctgg ctggcttacc gccagtagtc
     5581 agagcgaaga tgattcgcct gattgataaa cttaaaacca acgctactgc attacgagag
     5641 ccagatagca aaccactccg ggatggatta tttgagttaa ggacgatggg gacggatatt
     5701 gccagaggtc tttatgttta tcagaaaggc aggaatatct atctacttag agtctttatt
     5761 aaaaagactc agaaaacccc atcgggtgaa atatctatgg cctttgaacg tcttaaggag
     5821 atgctaaatg agtaaagtta aaggtatcgt ttgggagcat gttaggaatg aattgatgtc
     5881 taatcctgag gttcaagcag cctatgaagc agaggagcgc aaggaaagac ttcaagctat
     5941 gctcgctgaa tggcgtcatc acgcaggatt aacaagagcc caggtggcag aacgcatggg
     6001 tgttactcca cctaccgttt ctcgcatgga atctaatgtg attaaggcta gcattgaaac
     6061 actggctcga tacgcaagag catgcggtgt taagcaccct cagatcacgt tgtaattttc
     6121 aaggccgctt taagcagcct tttgatgcgt gcaagatcgc ttatgttgaa caaggagttt
     6181 ttttgcaaaa aaccatcatt tcacacatat gagataatcc taagggatat aaataggcta
     6241 attttgccca acgcgccaaa aatgtaaagt tagcatttac agcttgtaag ccattgttct
     6301 agtttcacaa cggcaaattg atgttaagtc taaaagaccg tgataactca ttgtggaaga
     6361 ttgagagcta aatgtaacaa tagaggacat tgttagtcca agatttaagg ggtgaatatg
     6421 tcacccctaa gataaaacat ttacagccat atctaatacc tgcaaggtgc aaaaatatcg
     6481 agttggctct gatagacggt ggtttttaca ttcataagtc ctagcaaact gtcgaacaaa
     6541 ttgtcgtggg aaaagccgcc ctgttctgct cgctgtgcaa cacaagtcat gttcaactga
     6601 ttatcttggc taaagtcatt agatacccaa gccagcattg gtacgctagt ttgttctttc
     6661 ggtgcaatac tatagggcgc accatgtaaa tacatgccct tttcacccaa agactcacca
     6721 tggtcagaga gatacagcat tgcagtatcg aacatatcct gcttaccttt taatttattc
     6781 accacttcac tgagaataaa atccgtatat agaatagtgt tgtcgtaggt gttaatcagt
     6841 tcttcttgac tgcaattttg aatatcactg cgcggacaat ccggtataaa tttacgatgc
     6901 tctggcgggt atctaagata ataagttggt ccatgactac caatgatatg caaaaaaatt
     6961 actgtatctt gacttggtgc tactgccaga attttatcta atttgttgag caatacttgg
     7021 tcaaaacaat attggccaga acacagcttc ggatcactct tcaaatctat cgtgagattt
     7081 tcaacctgat cacacacacc tttacagcca gaatcattat caaaccactg tacttttata
     7141 ccactatgac ttaacacatc aatcactgtg tcttgagcat tagggaaggt gcgaacaagt
     7201 tcctgatatg agatcatcat attcatccgg agcgcatccc aaagggacat catgagccat
     7261 caactcacct tcgccgatag tgaattcagc actaagcgcc gtcagacccg aaaagagatt
     7321 ttcctctccc gcatggagca gattctgcca tggcagaata tgaccgctgt catcgagccg
     7381 ttttatccca aggcgggcaa tggccgacgg ccctatccgc tggagaccat gctgcgtatt
     7441 cactgcatgc agcattggta caacctgagc gacggcgcta tggaagatgc cctgtacgaa
     7501 atcgcctcca tgcgcctgtt tgcccgatta tccctggata gcgccctgcc ggatcgcacc
     7561 actatcatga atttccgcca cctgctcgag cagcatcaac tggcccgtca attgttcaag
     7621 accatcaatc gctggctggc cgaagcaggc gtcatgatga cccaaggcac tttggtggat
     7681 gccaccatca ttgaggcgac ccagctctac caagaacaaa gagcagcaac gcgatccgga
     7741 g
//
