LOCUS       CP122742               12240 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC1745 plasmid unnamed2, complete
            sequence.
ACCESSION   CP122742
VERSION     CP122742.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549622
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 272x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 12:23:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,733
            CDSs (total)                      :: 5,605
            Genes (coding)                    :: 4,395
            CDSs (with protein)               :: 4,395
            Genes (RNA)                       :: 128
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 1,210
            CDSs (without protein)            :: 1,210
            Pseudo Genes (ambiguous residues) :: 0 of 1,210
            Pseudo Genes (frameshifted)       :: 1,054 of 1,210
            Pseudo Genes (incomplete)         :: 160 of 1,210
            Pseudo Genes (internal stop)      :: 64 of 1,210
            Pseudo Genes (multiple problems)  :: 63 of 1,210
            Pseudo Genes (short protein)      :: 4 of 1,210
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC1745"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            16..339
                     /locus_tag="QDY25_26680"
     CDS             16..339
                     /locus_tag="QDY25_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WHG96552.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            548..979
                     /locus_tag="QDY25_26685"
                     /pseudo=""
     CDS             548..979
                     /locus_tag="QDY25_26685"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1017..1535
                     /locus_tag="QDY25_26690"
     CDS             1017..1535
                     /locus_tag="QDY25_26690"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WHG96545.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            1542..1775
                     /locus_tag="QDY25_26695"
     CDS             1542..1775
                     /locus_tag="QDY25_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WHG96546.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2138..3385)
                     /locus_tag="QDY25_26700"
     CDS             complement(2138..3385)
                     /locus_tag="QDY25_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WHG96547.1"
                     /translation="MIKFVDLFSGTGGIRLGFEQAMKEIGLSTKCVKSSEIDKKACETY
                     KLNFDEESYCDIHDVDIDESFDVLLAGFPCQAFSYAGKQLGFADTRGTLFFEVERIIKK
                     NKPKLCLLENVRGLTSHDKGRTFSTILKSLHNLGYHVEYRLLNTSNFGVPQNRVRIYII
                     ASLEKEVTLTIPNDLGAKDTHNYKPSKGGHDFYTESKVSDILEDSPDESYDCSDWFKQR
                     LEDIFEGDLNKVHGIRLIDTRNGNSLHSWDLGLKGECSNDEIIFMNLLIANRRKHIFGT
                     HQDGKALSKAQIETFFKHSETTNIIKNLIKKGYLKEKNGLYNPVCGNMSFEVFKFLDPN
                     KVSITLTASDANRLGVYHNNRLRRLTPRECARLQGYPDSYILHPKDSMAYKQLGNAVSV
                     PVIKNIFLDIMKNNFI"
                     /name="DNA cytosine methyltransferase"
     gene            complement(3382..4485)
                     /locus_tag="QDY25_26705"
     CDS             complement(3382..4485)
                     /locus_tag="QDY25_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG96548.1"
                     /translation="MQREKPPEWREKQKGSRGIRRGQRYRLVFQFPIRQELENSACLYW
                     PKELAERAASISAISPLIETQDEFLSILTISTNKPTSCFDAVRLCNKISPNLFVKHLMV
                     LSDIGGERLHRFFKDLDKIYPDRIMEFNIGNSSYSYQFNSNRAWTTKNLNVEKSRLLQP
                     VSDFTREMLDVCMLILWGGNTINNTNLPTEIENNCVLGNLIGNKEAIEQFVKERYIMVS
                     RQTGGATANDLGHICEIFIKEKLYKLIDNNISLDGHHIDGVTHNDKDLTTFDIVAKNTT
                     TKKCVGIEISFQVTTNSTIERKAGLAKSRRDLLNSRGHKIAYVIDGSGNFQRSNAIKTI
                     INHSDMTVNFSDNGINSLATFICETLK"
                     /name="hypothetical protein"
     gene            complement(4555..5763)
                     /locus_tag="QDY25_26710"
     CDS             complement(4555..5763)
                     /locus_tag="QDY25_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WHG96549.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLA
                     CGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVH
                     LVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHP
                     HVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSHITTE
                     SQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFR
                     YLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGM
                     DKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNARDISLLRY
                     MPA"
                     /name="IS91 family transposase"
     gene            complement(5744..6016)
                     /locus_tag="QDY25_26715"
     CDS             complement(5744..6016)
                     /locus_tag="QDY25_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHG96550.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSCT
                     VCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
                     /name="hypothetical protein"
     gene            6094..6327
                     /gene="tnpB"
                     /locus_tag="QDY25_26720"
                     /pseudo=""
     CDS             6094..6327
                     /gene="tnpB"
                     /locus_tag="QDY25_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial on complete
                     genome; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /name="tnpB"
     gene            6347..7920
                     /locus_tag="QDY25_26725"
                     /pseudo=""
     CDS             6347..7920
                     /locus_tag="QDY25_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /name="IS66 family transposase"
     gene            complement(7950..8105)
                     /locus_tag="QDY25_26730"
                     /pseudo=""
     CDS             complement(7950..8105)
                     /locus_tag="QDY25_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            complement(8186..8623)
                     /locus_tag="QDY25_26735"
     CDS             complement(8186..8623)
                     /locus_tag="QDY25_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WHG96541.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSKGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="entry exclusion protein 1"
     gene            8883..10451
                     /gene="cea"
                     /locus_tag="QDY25_26740"
     CDS             8883..10451
                     /gene="cea"
                     /locus_tag="QDY25_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WHG96542.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETKRQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(10448..10675)
                     /locus_tag="QDY25_26745"
     CDS             complement(10448..10675)
                     /locus_tag="QDY25_26745"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WHG96551.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            10837..10974
                     /locus_tag="QDY25_26750"
     CDS             10837..10974
                     /locus_tag="QDY25_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_component:
                     GO:0019867 - outer membrane [Evidence IEA]; GO_process:
                     GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WHG96543.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(12001..12192)
                     /locus_tag="QDY25_26755"
     CDS             complement(12001..12192)
                     /locus_tag="QDY25_26755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WHG96544.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          11829..11916
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(11260..11815)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          330..1879
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaaacgcg agaggatgct cacaatacgg gttactgatg atgaacatgc ccggttactt
       61 gaacgctgtg agggtaaaca actggcggta tggatgcggc gggtctgcct gggggagccg
      121 gttgcccgtt ccggaaaact gccgacactg gcgccgccgt tactgcgtca gctggccgcc
      181 atcggaaata acctgaatca gacagcccgt aaggtgaaca gcgggcagtg gtcttccggt
      241 gaccgggttc aggtggtggc cgcactgatg gccatcgggg atgagctgcg ccggctgcgt
      301 ctggctgtca gggaacaggg ggcgcgggat gatagttaaa tttcatgcca ggggaaaagg
      361 tggtggcagt ggtccggttg attacctgct ggggagggag cgtaaccgcg aaggcgcaac
      421 ggtgcttcag ggtaatccgg aagaagtccg ggaactcatc gatgccacgc catttgcgaa
      481 gaaatacacg tccggtgttc tgtcgttcgc ggagaaggag ctgccgccgg gaggacgtga
      541 aaaagtgatg gcgagctttg agcgtgtact gatgcccggt ctcgaaaaaa atcagtacag
      601 catcctgtgg gtggagcacc aggacaaggg acggcttgag ctgaattttg tcattccgaa
      661 catggagcta cagaccggaa aacgcctcca gccgtactac gaccgcgcag acaggcctag
      721 aattgatgcc tggcagacgc tggtgaatca ccattacggg ctgcatgacc cgaacgcccc
      781 ggagaaccgc aggacgctga cactccctga taacctgcct gaaacgaaac aggcgcttgc
      841 tgagggcgtc acgcgaggta tagatgcact ttaccatgcc ggagagataa aaggccgtca
      901 ggatgtgatt caggcgctca ctgaggcggg gctggaagtg gtcagggtga cgcgaagcag
      961 tatcagcatt gcagatccga acggcgggaa gaatatcagg ctgaaaggag cattttatga
     1021 gcaatctttt gcagacgggc gcggagttcg agaaaaagct gaaagagaga gccgaatcta
     1081 cagagaaaat gctgaacaac gagttcagga ggctcggcga atctgtaagc gaggctgtga
     1141 catcaaacga gacgaaaatc agagacgcta tagccctgtt cacagcctcg acagaggaat
     1201 cgctggaaaa acaccgggaa ggggtgaaag aggcgatgat gcagcacagg agggacgtgt
     1261 taaagctggc agggaatacg ggcatgatgt tactggggat agtctttctc ctgtttaccg
     1321 cgagtggcgg gacgctctgg tatcttggag ggaggataca ggcgaacctg gaagaaatca
     1381 ggaagcagga agagacattg cagaaactga acgcgaagac atggggcgtg gagtttgtgc
     1441 aggacgggaa caggaaattc cttgtccttc cgtacgggaa atcagcggag gtgattccct
     1501 ttcaggggaa agagtgggta catctgaagg agtgacacag agtgacagag ctggaaacac
     1561 atttgctgaa cgccttagag caactgcaac aggactatat gcagcggctg agcgaatggg
     1621 agagcgcctt cgtggaattg cagaagatgt tttcgcttac gcaacgggac aacgcgatgc
     1681 tgaacgagcg ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag cagacagaac
     1741 gcttgagcca gttatacagc gagaactgga gataagagag gaacggctga tacaggagcg
     1801 cgaacatgtg ttatccctgg aacgggagcg tcagccggaa atacaggaac gcacgctgga
     1861 tggcccttcg ctgggatggt gaaaccatga aaaatggcag cttcagtgga ttaagtgggg
     1921 gtaatgtggc ctgtaccctc tggttgcata ggtattcata cggttaaaat ttatcaggcg
     1981 cgatcgcggc agtttttcgg gtggtttgtt gccattttta cctgtctgct gccgtgatcg
     2041 cgctgaacgc gttttagcgg tgcgtacaat gcgacgttat ggtaaatctg ttaaatttaa
     2101 ttaaataacc atagagcagt tttgctagta atatggttta tataaagtta ttcttcatta
     2161 tatcaagaaa aatattttta atgactggta cgcttacagc attccctaat tgtttatatg
     2221 ccatgctatc ttttgggtgt aagatatatg agtctgggta accttgtagt cttgcgcatt
     2281 ctcttggcgt aaggcgtcgc agtctattgt tgtgatagac tccaagtcta ttggcatcag
     2341 atgccgtaag tgtaattgac actttatttg ggtcaaggaa tttgaatact tcaaaagaca
     2401 tatttccgca aactgggttg tatagtccgt ttttctcttt aaggtaaccc tttttaatta
     2461 ggttttttat tatgttggtg gtttcgctat gtttaaaaaa tgtttcaatt tgtgcttttg
     2521 acaaagcctt gccgtcttgg tgtgtgccaa aaatatgctt tctacggttt gcaattaata
     2581 aattcatgaa aattatttca tcatttgagc actcgccttt tagcccaaga tcccaagagt
     2641 gcaaggagtt tccgttcctt gtgtcaatta gcctgattcc atgaacttta tttaggtcac
     2701 cttcaaaaat gtcttcaagg cgttgcttga accaatcgct gcaatcatat gactcgtctg
     2761 gactatcctc tagtatgtca gaaacttttg attctgtata aaaatcatga ccacctttgg
     2821 atggtttgta attgtgcgta tctttggctc caagatcatt aggaatagta agtgttacct
     2881 ctttttctaa tgaggctatt atatatattc taaccctatt ttgagggaca ccaaaattac
     2941 ttgtgtttaa tagtctgtat tcaacatgat accctaaatt atgaagagat tttaatattg
     3001 ttgaaaatgt tcttccttta tcatgtgaag ttaatccacg cacattctcc aaaagacata
     3061 acttaggctt gtttttcttt atgattcttt ctacttcaaa aaataatgtg cctcgggtgt
     3121 ctgcaaaacc aagttgtttt ccagcataag agaacgcctg acaaggaaat cctgccaata
     3181 aaacatcgaa gctttcatct atatcaacat catgtatatc acaatatgat tcttcgtcga
     3241 agttaagttt gtatgtttcg catgcttttt tgtcaatttc tgagcttttt acgcattttg
     3301 ttgacaatcc aatttctttc atggcttgct caaaaccaag tctaatacca cctgtgccag
     3361 aaaaaaggtc aacaaattta atcatttcaa tgtctcgcag atgaatgttg ctagtgaatt
     3421 tataccatta tctgaaaagt taacggtcat gtcactatgg ttaattattg ttttgatggc
     3481 atttgaacgc tgaaaattac cagacccatc aatcacatat gctattttat gtcctcttga
     3541 attgagcaaa tctcttctgc ttttggctaa tcctgctttt ctttctattg ttgagtttgt
     3601 tgtaacttga aaacttattt caataccaac acattttttt gttgttgtgt tttttgctac
     3661 aatatcaaaa gtcgtaagat ctttatcgtt atgagttaca ccatcgatat ggtgtccatc
     3721 tagagatata ttgttatcaa taagcttgta aagtttttct tttataaaga tctcgcatat
     3781 gtgtccaaga tcatttgccg ttgcacctcc tgtttgcctg ctaaccatta tatatctttc
     3841 ttttacaaac tgttctattg cttctttgtt tccaattaaa ttccccaaaa cacagttatt
     3901 ttctatttcg gttggaagat ttgtattgtt tatagtatta cctccccata ggatcagcat
     3961 gcaaacatca agcatttcac gtgtaaaatc acttacaggt tgcaagagcc gtgatttttc
     4021 cacgtttaaa ttcttggttg tccatgctct attgctattg aattggtaag agtaagagct
     4081 atttcctata ttgaactcca ttattctgtc ggggtagatc ttatctaaat ctttaaagaa
     4141 gcgatgaagt ctttcgcctc caatatctga caaaaccatt aagtgcttga caaataggtt
     4201 gggtgatatc ttattgcata accgtacggc atcaaaacaa gatgtaggct tatttgttga
     4261 tattgttagt attgataaaa attcatcttg ggtctctata agagggctta tggcgctaat
     4321 agatgctgct ctttctgcaa gttctttggg ccaatataag catgcgctat tttctaattc
     4381 ttgacgtata ggaaattgaa aaaccagacg ataccgctgt ccacgcctta tgccacgtga
     4441 gcctttctgc ttttccctcc actccggtgg tttctctctc tgcatggtcg atttttatac
     4501 cgtaacaggc tgttttttat acctgcaccg attcccgggc acacttagcc tgtgtcaggc
     4561 cggcatatac ctcagcagac tgatatcccg cgcgtttttc ttcagccctg atacattcag
     4621 ccccgcaatg gcccggcggt ataccatccg gccgccacac agcacgcatt cgaacgggtc
     4681 acgactcagg aactgtttca ccatttgtgc atagcacact ttcgccactg gttccggctt
     4741 atccatcccc agagcacggt acacctgcgg cagcttctct ccacacaccc ggttggcaag
     4801 gaacccgaag tatctcacca tcttaaaaaa cttctccggg atgtgctgtt tcagcctcgc
     4861 gaccagctcc cgctgtgtca gcgtttccgt cgccgtttct cccgttttgt ggtccaggta
     4921 acggaagctc agactcgctc ctccgttgta atgcgccagc cgggaagccg ctattggtgg
     4981 cttcttcaga taacgaccca ggtagcgtgc cgtattccgc cctccggctg tcttcttcga
     5041 catgtacaca tgccagtatt ttccgccggc tttcagcacc aggcttctcc actgtgattc
     5101 cgtcgtgata tgtgacaacg attccggcat cgccagacct tctgaccacg ctttcagaag
     5161 cagctgccgc atattccaca tccaccgcga acgcatcgcg tctttcagga agctcagctt
     5221 tttccagtgt ccgtgtttat tcagacctcc gcaggtcact gacacatgca catgcggatg
     5281 ccagttgaga cgacggccat acgtatggat ggcgcagaag ataccgggtt ccagaccccg
     5341 tttccgggct gcatacagca gattctccac cgccagacgg cacacgtcat tcagcagcca
     5401 gcggttgctt tcgaacaccg gccacagcgt gtccggcagg gtgaagacca ggtgcaccca
     5461 gtcgcagtca ggaagacgat tcagctgtgt cgctatccac aggtctgtgg ccttttttcc
     5521 gcaggacggg caggcacggc tgccacatga gttagtgacg tatctgacgt gggggcactc
     5581 aggtttatcg cagttgtatt ccttcacacc cagtatccgc gtgccgcagg ccagcatttt
     5641 tgtgacagcc tcaacctcga tatcgcgcag gccgcccgca tccaggaagg atgtccagca
     5701 ctggttggct gtgaacaggc gtttcagcgg tcggggctga aaatcaccac cacttcgggt
     5761 catccgcctt catctccgct tcagtttcgt ataaatcaaa acgacggcag gtatggcaga
     5821 acgtgacgta ttcgtacggt gaatccgggt acattttgtg gaaggtttcc cagaaacaga
     5881 ccgtacagga gggatgaccg gcgataaagt ctgtaaatac agactgataa gggtgattat
     5941 tggccctggc gacggctttc agaacctctt tcaccattct ggtgtggact ttctggtgct
     6001 ccaggttgtg tgacatggga actcattctg gatggttact ctgaaagctc atattctgcc
     6061 acaccacgat ttgcagccgc caggctgccg tggttctccg ggcatctgtt catcttccgc
     6121 ggacgccggg gtgaccagat aaaagtgctg tgggctgaca gtgacggact gtgcctcttc
     6181 accaaacgcc tggagcgggg ccgcttcgtc tggccggtca cccgcgatgg aaaggttcac
     6241 cttactccgg ctcagttgtc catgcttctc gaaggcatcg actggaagca cccgaaacga
     6301 acggaacgcg ctggcatccg catataaccc gttgtaaagt gaggatatgg acacctcact
     6361 tgctcatgag aatgcccgcc tgcgggcact gttgcagacg caacaggaca ccatccgcca
     6421 gatggcgaaa tacaaccgcc tgctctcaca gcgggtggcg gcttatgctt ccgaaatcaa
     6481 ccggctgaag gcgctggttg cgaaactgca gcgcatgcag ttcggtaaaa gctcagaaaa
     6541 acttcgcgct aaaaccgaac ggcagataca ggaagcccag gagcgaatca gcgcacttca
     6601 ggaagaaatg gcggaaacgc tggatgagca atatgacccg gtactgccat ccgccctgcg
     6661 ccagtcttca gcccgtaagc cgttaccggc ctcacttccc cgtgaaaccc gggtcatccg
     6721 gccggaagag gaatgctgcc cggcctgtgg tggtgacctc agtcctctgg ggtgtgatgt
     6781 gtcagagcaa ctggagctta tcagcagcgc ctttaaggtt atcgaaacac aacgtccgaa
     6841 actggcctgt tgccggtgcg accatatcgt gcaggcaaca gtaccttcaa aacccattgc
     6901 acgcagttat gccggagcgg ggcttctggc ccatgttgtt accgggaaat atgcagacca
     6961 tctgccgtta taccgccagt cagaaatata ccgtcgccag ggcgtggagc tgagccgcgc
     7021 cacgctgggg cgctggacag agtcgccgtt gctgaactgc tggagccgct gtatgacgtc
     7081 ctgcgccagt atgtgctgat gcccggtaaa gtccatgcgg atgatatccc cgttccggtc
     7141 caggagccgg gcagtggtaa aacaagaact gcccggctgt gggtctatgt ccgtgatgac
     7201 cgtaacgccg gttcacagat gcccccggcg gtctggttcg cgtacagccc ggaccggaaa
     7261 ggtatccatc cacagaatca cctggccggt tacagcggtg tgcttcaggc cgatgcttac
     7321 ggtggttacc gggcgttata cgaatccggc agaataacgg aagccgcgtg tatggcccat
     7381 gcccggagaa aaatccacga tgtgcatgca agagtgccaa ccgacatcac cacggaagcc
     7441 ctgcagcgta tcggtgaact gtatgtcatc gaggcagaag tccggggatg ttcagcagaa
     7501 cagcgtctgg cagcaagaaa agccagagcg gctccactga tgcagtcact gtatgactgg
     7561 atacaggaac agatgaaaac actgtcgcgt cactcggata cggcaaaagc gttcgcatac
     7621 ctgctgaaac agtgggaggc cctgaacgtg tactgcagta atggctgggt ggaaatcgac
     7681 aacaacatcg cagagaacgc cttacgggga gtggccgtag gccggaaaaa ctggctcttc
     7741 gcgggttccg acagcggtgg cgaacatgcg gcggtgttgt actcgctgat cggcacatgc
     7801 cgtctgaaca atgtggaacc agaaaaatgg ctgcgttacg tcattgagca tatccaggat
     7861 tggccggcaa accgggtacg cgatctgttg ccctggaaag ttgatctgac ctctcagtaa
     7921 atatcaatac ggttctggcg agccgcttac ttttgggtgg aaaagttggg atcaaaaccg
     7981 tcatcagaag catatacgta atgccgggta tgttgttcca cgctgccctt tggggtacag
     8041 gctgtgagag ctactgttgc tgttatcatg gcgataagac aaagtttgtt tccggtacgt
     8101 ctcatgagtg tctccctacc ttaaagtatt tattctcggt gagttattat ccgaagacta
     8161 ttttatcatc agtctctgac agcgttcagc gaccgaacaa cctggaccag aatccccgtt
     8221 tcttttccag ttccagtgcc tgcctgagct gggctatctc attttgtagc tgttcccgtt
     8281 ctgcttcctg gcgtctgcga tccatatcct gtgcctgttt atcctcaagc atcagcgtca
     8341 ggcattgttt cagctcattc agctcccgga gaatgcgttc tgtctgctga tcatgtggat
     8401 tttctgcatg tcccttactg tgcctttctg gtgtctcatt ctgctttaat tcgccgtatg
     8461 cccggatcag ttcactggtt tcaaactccc gtcgtccgtc cctgccggta cggtaactga
     8521 caaggcctgc gcgcatatca cggtaaaact gtgaacgcga tctgcctgtc atttttagtg
     8581 cgtcccggag tgtgtgccat gccataaagt gacagtgtcc catagatgtc tcatctcata
     8641 gtttcagtaa aacataatga ggtctgagaa cggtaatgtt tgtgctggtt tttgtggcat
     8701 cgggcgagaa tagcgcgtgg tgtgaaagac tgtttttttg atcgttttca caaaaatgga
     8761 agtccacagt cttgacaggg aaaatgcagc ggcgtagctt ttatgctgta tataaaacca
     8821 gtggttatat gtacagtatt tatttttaac ttattgtttt aaaagtcaaa gaggatttta
     8881 taatggaaac cgcggtagcg tactataaag atggtgttcc ttatgatgat aagggacagg
     8941 taattattac tcttttgaat ggtactcctg acgggagtgg ctctggcggc ggaggtggaa
     9001 aaggaggcag taaaagtgaa agttctgcag ctattcatgc aactgctaaa tggtctactg
     9061 ctcaattaaa gaaaacacag gcagagcagg ctgcccgggc aaaagctgca gcggaagcac
     9121 aggcgaaagc aaaggcaaac agggatgcgc tgactcagcg cctgaaggat atcgtgaatg
     9181 aggctcttcg tcacaatgcc tcacgtacgc cttcagcaac agagcttgct catgctaata
     9241 atgcagctat gcaggcggaa gcagagcgtt tgcgccttgc gaaagcagaa gaaaaagccc
     9301 gtaaagaagc ggaagcagca gaaaaggctt ttcaggaagc agaacaacga cgtaaagaga
     9361 ttgaacggga gaaggctgaa acgaaacgcc agttgaaact ggctgaagct gaagagaaac
     9421 gactggctgc attgagtgaa gaagctaaag ctgttgagat cgcccaaaaa aaactttctg
     9481 ctgcacaatc tgaagtggtg aaaatggatg gagagattaa gactctcaat tctcgtttaa
     9541 gctccagtat ccatgcccgt gatgcagaaa tgaaaacgct cgctggaaaa cgaaatgaac
     9601 tggctcaggc atccgctaaa tataaagaac tggatgagct ggtcaaaaaa ctatcaccaa
     9661 gagccaatga tccgcttcag aaccgtcctt tttttgaagc aaccagacga cgggttgggg
     9721 ccggtaagat tagagaagaa aaacaaaaac aggtaacagc atcagaaaca cgtattaacc
     9781 ggataaatgc tgatataact cagatccaga aggctatttc tcaggtcagt aataatcgta
     9841 atgccggtat cgctcgtgtt catgaagctg aagaaaattt gaaaaaagca cagaataatc
     9901 tccttaattc acagattaag gatgctgttg atgcaacagt tagcttttat caaacgctga
     9961 ctgaaaaata tggtgaaaaa tattcgaaaa tggcacagga acttgctgat aagtctaaag
    10021 gtaagaaaat cggcaatgtg aatgaagctc tcgctgcttt tgaaaaatac aaggatgttt
    10081 taaataagaa attcagcaaa gccgatcgtg atgctatttt taatgcgttg gcatcggtga
    10141 agtatgatga ctgggctaaa catttagatc agtttgccaa gtacttgaag attacggggc
    10201 atgtttcttt tggatatgat gtggtatctg atatcctaaa aattaaggat acaggtgact
    10261 ggaagccact atttcttaca ttagagaaga aagctgcaga tgcaggggtg agttatgttg
    10321 ttgctttact ttttagcttg cttgctggaa ctacattagg tatttggggt attgctattg
    10381 ttacaggaat tctatgctcc tatattgata agaataaact taatactata aatgaggtgt
    10441 tagggattta attattcttt attgatataa aaagtcctag caatccaaat gggattgcta
    10501 ggaccaaaca aagtagatta tatagcataa ataggtttaa ttttgctacg ggggcgttat
    10561 ttaggttttt tcttctttcg aaaaaatctt tctttatgaa gttaaaagct atgtattcaa
    10621 tagcatattt tgaatatgga catagaatag tgcttatcac tattgcatat agcatcttat
    10681 ctgacacaag gaaataatac ccttcgctgt tttttgttat aaggtatata tatataagtg
    10741 tgcagtacag gccaaataaa atatttttta tgtagtatct taagctcata aattaaacct
    10801 cgccatatat tcttttcatt ttataaggat cgagttatga ggaaaagatt ttttgtggga
    10861 atattcgcga taaacctcct tgttggatgt caggctaact atatacgtga tgttcaggga
    10921 gggaccatcg caccatcctc ctcttctaaa ctgacgggga tcgcggttca gtagaaaaga
    10981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
    11041 aaccaccgct accaacggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
    11101 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
    11161 cgggccacta cttcaagaac tctgtagcac cgtttgtgcc atcatcgctc tgctaatccg
    11221 gttaccagtg gctgctgcca gtggcgttaa ggcgtgcctt accgggttgg actcaagacg
    11281 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    11341 cttggagcga acgacctaca ccgaactgag ataccaacag cgtgagctat gagaaagcgc
    11401 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    11461 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    11521 tcgccacctc tgacttgagc gtctattttt gtgatgctcg tcaggggggc ggagcctatg
    11581 gaaaaacgcc tgctacgtgg ccttcttcct gttcctggtc ttttgctcac atgttctttc
    11641 cggccttatc ccctgattct gtagataact gtgttaccgt ttttgtgtga gtcagtaccg
    11701 ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaaaagcgcc
    11761 tggacgtgca ttttctcctt acgcatctgt gcggcatttc acacccggca tggcgtactt
    11821 ttcatacaat ccgcactgat gccgcatggt taagccagta tacactccgc tatcgctacg
    11881 tgactgggtc agggctgcgc cccgacaccc gctaaaacct gctgacgcgc cctgacgggc
    11941 ttgtcagctc ccggcatccg ctcacagaca agctgtgacc gtctccggga gctgcatgtg
    12001 tcagaggttt tcaccgtcat ccccgaaacg tgcgaggcag ctgcggtaaa gctcatcggc
    12061 gtggtcgtga agcgattcac aaatatcggc ctgttcatct gcgtccagtt cgttgagctt
    12121 ctccagcagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
    12181 ctgtttagtc actgatgcct ccgtgtaagg gggatttctg ttcatggggt aatgataccg
//
