LOCUS       CP122703                7480 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC4063 plasmid unnamed3, complete
            sequence.
ACCESSION   CP122703
VERSION     CP122703.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549640
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 290x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 12:02:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,290
            CDSs (total)                      :: 5,167
            Genes (coding)                    :: 4,141
            CDSs (with protein)               :: 4,141
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 1,026
            CDSs (without protein)            :: 1,026
            Pseudo Genes (ambiguous residues) :: 0 of 1,026
            Pseudo Genes (frameshifted)       :: 898 of 1,026
            Pseudo Genes (incomplete)         :: 127 of 1,026
            Pseudo Genes (internal stop)      :: 44 of 1,026
            Pseudo Genes (multiple problems)  :: 45 of 1,026
            Pseudo Genes (short protein)      :: 3 of 1,026
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC4063"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            join(7116..7480,1)
                     /locus_tag="QDW63_26410"
     CDS             join(7116..7480,1)
                     /locus_tag="QDW63_26410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHH25681.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDAEVLRFDNF
                     DDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVTPPV"
                     /name="hypothetical protein"
     gene            1..1188
                     /locus_tag="QDW63_26415"
     CDS             1..1188
                     /locus_tag="QDW63_26415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element IS91 family transposase"
                     /protein_id="WHH25682.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVTESVTKIMACGTTL
                     MGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTL
                     PCQYWSLVFHNRWLLAEMSRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHIHLS
                     TTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPELVIPDELAVEGNSKRDWNR
                     FLDTHYRRGWNVNISRVMDNATHVAVYFGSYLKKPPVSMSRLEHYAGQDEIGLRYNSHR
                     TKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEEVVYVITETVRKTA
                     MQIRWRGMYQRLLKVDPLKCILCGSQMRFTGLKRGYRLAELVLMHERLARQQVCG"
                     /name="IS91-like element IS91 family transposase"
     gene            1737..2378
                     /locus_tag="QDW63_26420"
     CDS             1737..2378
                     /locus_tag="QDW63_26420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHH25683.1"
                     /translation="MEKSRLLQPVSDFTREMLDVCMLILWGGNTINNTNLPTEIENNCV
                     LGNLIGNKEAIEQFVKERYIMVSRQTGGATANDLGHICEIFIKEKLYKLIDNNISLDGH
                     HIDGVTHNDKDLTTFDIVAKNTTTKKCVGIEISFQVTTNSTIERKAGLAKSRRDLLNSR
                     GHKIAYVIDGSGNFQRSNAIKTIINHSDMTVNFSDNGINSLATFICETLK"
                     /name="hypothetical protein"
     gene            2375..3622
                     /locus_tag="QDW63_26425"
     CDS             2375..3622
                     /locus_tag="QDW63_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WHH25684.1"
                     /translation="MIKFVDLFSGTGGIRLGFEQAMKEIGLSTKCVKSSEIDKKACETY
                     KLNFDEESYCDIHDVDIDESFDVLLAGFPCQAFSYAGKQLGFADTRGTLFFEVERIIKK
                     NKPKLCLLENVRGLTSHDKGRTFSTILKSLHNLGYHVEYRLLNTSNFGVPQNRVRIYII
                     ASLEKEVTLTIPNDLGAKDTHNYKPSKGGHDFYTESKVSDILEDSPDESYDCSDWFKQR
                     LEDIFEGDLNKVHGIRLIDTRNGNSLHSWDLGLKGECSNDEIIFMNLLIANRRKHIFGT
                     HQDGKALSKAQIETFFKHSETTNIIKNLIKKGYLKEKNGLYNPVCGNMSFEVFKFLDPN
                     KVSITLTASDANRLGVYHNNRLRRLTPRECARLQGYPDSYILHPKDSMAYKQLGNAVSV
                     PVIKNIFLDIMKNNFI"
                     /name="DNA cytosine methyltransferase"
     gene            complement(3985..4218)
                     /locus_tag="QDW63_26430"
     CDS             complement(3985..4218)
                     /locus_tag="QDW63_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WHH25678.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4225..4743)
                     /locus_tag="QDW63_26435"
     CDS             complement(4225..4743)
                     /locus_tag="QDW63_26435"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WHH25679.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            complement(4781..5212)
                     /locus_tag="QDW63_26440"
                     /pseudo=""
     CDS             complement(4781..5212)
                     /locus_tag="QDW63_26440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5421..5745)
                     /locus_tag="QDW63_26445"
                     /pseudo=""
     CDS             complement(5421..5745)
                     /locus_tag="QDW63_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5810..6001
                     /locus_tag="QDW63_26450"
     CDS             5810..6001
                     /locus_tag="QDW63_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WHH25680.1"
                     /translation="MTKQEKTAINMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6080..6164)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          6175..6732
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.583"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3882..5431)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.649"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5428..5606)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.333"
                     /coverage="98.0"
                     /evalue="1.48e-51"
     Typing          complement(5609..5775)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.214"
                     /coverage="98.0"
                     /evalue="1.48e-51"
ORIGIN
        1 atgcttcccc gttttgccga tatttttcag cagggtaacc gctggcttaa ctggctggag
       61 aaacagccgg aaggttcagt gcgtccggtg gtgactgagt cagtgacaaa aatcatggca
      121 tgcgggacca cgctgatggg ctacacgcaa tggtgctgtt cgtcaccgga ctgttgccac
      181 accaaaaagg tctgcttccg gtgtaaaagc cgctcctgtc cgcactgcgg ggtgaaggct
      241 ggcgcacagt ggatacagta tctgctgagc ctggtccccg actgcccgtg gcagcatatt
      301 gtgttcacac ttccctgcca gtactggtcc ctggtgttcc acaaccggtg gttactggca
      361 gagatgagcc gcattgcagc ggatgtgata ctggaaatct gccatcagac agatgtggag
      421 ccggggatat tcacggtgat ccacacatgg gggcgtgacc agcagtggca tccgcatatc
      481 catttatcga caactgccgg tggtgtgacg tcgggccaca cctggaaaaa tcttcatttt
      541 tacgcgcgta aggtgatgag catgtggcgt taccggataa cgcggctact gtcccggaaa
      601 tacccggagc tggtaatacc ggatgaactg gcagtggaag gaaacagcaa acgggactgg
      661 aatcgcttcc tggacacgca ttaccgccgc ggctggaatg tcaacatatc cagggtgatg
      721 gataacgcca cacatgtggc ggtgtacttc ggctcttacc tgaaaaagcc accggtgtcg
      781 atgagtcggc tggagcatta tgccggtcag gatgaaatcg gtctgcgtta caacagtcac
      841 cgtacaaaac gggaagaata cctgttgatg agtggagatg agttcatgga aaggttctcc
      901 tggcatgtag cagataaggg gttccgtatg gtgaggtact acggtttcct gagtccggtg
      961 aagcgccgct tactggaaga agttgtgtac gtcataacgg agacggtgag aaaaacggcg
     1021 atgcaaatca ggtggagagg gatgtatcag aggttactga aggttgaccc gctgaagtgc
     1081 attctgtgcg gaagtcagat gcgttttacg gggctgaagc ggggttaccg actggcagag
     1141 ctggtcctga tgcatgagcg actggcacga cagcaggtgt gcggctgaga gccgcagagg
     1201 ggaagttgcg tccattttac cggaaacgga gcaaaaaacc gccattcata ccctgtatca
     1261 atcagtgtca tcctgtttaa tagtcgtttc cgctcatatg gtgcacaagg ggtgttgaag
     1321 aaacatccgt tttgtggtgc ttttttagtc ttttggggat ttaaattcct atcgatcaag
     1381 aattagaaaa tagcgcatgc ttatattggc ccaaagaact tgcagaaaga gcagcatcta
     1441 ttagcgccat aagccctctt atagagaccc aagatgaatt tttatcaata ctaacaatat
     1501 caacaaataa gcctacatct tgttttgatg ccgtacggtt atgcaataag atatcaccca
     1561 acctatttgt caagcactta atggttttgt cagatattgg aggcgaaaga cttcatcgct
     1621 tctttaaaga tttagataag atctaccccg acagaataat ggagttcaat ataggaaata
     1681 gctcttactc ttaccaattc aatagcaata gagcatggac aaccaagaat ttaaacgtgg
     1741 aaaaatcacg gctcttgcaa cctgtaagtg attttacacg tgaaatgctt gatgtttgca
     1801 tgctgatcct atggggaggt aatactataa acaatacaaa tcttccaacc gaaatagaaa
     1861 ataactgtgt tttggggaat ttaattggaa acaaagaagc aatagaacag tttgtaaaag
     1921 aaagatatat aatggttagc aggcaaacag gaggtgcaac ggcaaatgat cttggacaca
     1981 tatgcgagat ctttataaaa gaaaaacttt acaagcttat tgataacaat atatctctag
     2041 atggacacca tatcgatggt gtaactcata acgataaaga tcttacgact tttgatattg
     2101 tagcaaaaaa cacaacaaca aaaaaatgtg ttggtattga aataagtttt caagttacaa
     2161 caaactcaac aatagaaaga aaagcaggat tagccaaaag cagaagagat ttgctcaatt
     2221 caagaggaca taaaatagca tatgtgattg atgggtctgg taattttcag cgttcaaatg
     2281 ccatcaaaac aataattaac catagtgaca tgaccgttaa cttttcagat aatggtataa
     2341 attcactagc aacattcatc tgcgagacat tgaaatgatt aaatttgttg accttttttc
     2401 tggcacaggt ggtattagac ttggttttga gcaagccatg aaagaaattg gattgtcaac
     2461 aaaatgcgta aaaagctcag aaattgacaa aaaagcatgc gaaacataca aacttaactt
     2521 cgacgaagaa tcatattgtg atatacatga tgttgatata gatgaaagct tcgatgtttt
     2581 attggcagga tttccttgtc aggcgttctc ttatgctgga aaacaacttg gttttgcaga
     2641 cacccgaggc acattatttt ttgaagtaga aagaatcata aagaaaaaca agcctaagtt
     2701 atgtcttttg gagaatgtgc gtggattaac ttcacatgat aaaggaagaa cattttcaac
     2761 aatattaaaa tctcttcata atttagggta tcatgttgaa tacagactat taaacacaag
     2821 taattttggt gtccctcaaa atagggttag aatatatata atagcctcat tagaaaaaga
     2881 ggtaacactt actattccta atgatcttgg agccaaagat acgcacaatt acaaaccatc
     2941 caaaggtggt catgattttt atacagaatc aaaagtttct gacatactag aggatagtcc
     3001 agacgagtca tatgattgca gcgattggtt caagcaacgc cttgaagaca tttttgaagg
     3061 tgacctaaat aaagttcatg gaatcaggct aattgacaca aggaacggaa actccttgca
     3121 ctcttgggat cttgggctaa aaggcgagtg ctcaaatgat gaaataattt tcatgaattt
     3181 attaattgca aaccgtagaa agcatatttt tggcacacac caagacggca aggctttgtc
     3241 aaaagcacaa attgaaacat tttttaaaca tagcgaaacc accaacataa taaaaaacct
     3301 aattaaaaag ggttacctta aagagaaaaa cggactatac aacccagttt gcggaaatat
     3361 gtcttttgaa gtattcaaat tccttgaccc aaataaagtg tcaattacac ttacggcatc
     3421 tgatgccaat agacttggag tctatcacaa caatagactg cgacgcctta cgccaagaga
     3481 atgcgcaaga ctacaaggtt acccagactc atatatctta cacccaaaag atagcatggc
     3541 atataaacaa ttagggaatg ctgtaagcgt accagtcatt aaaaatattt ttcttgatat
     3601 aatgaagaat aactttatat aaaccatatt actagcaaaa ctgctctatg gttatttaat
     3661 taaatttaac agatttacca taacgtcgca ttacgcgcac cgctaaaacg cgttcagcgc
     3721 gatcacggca gcagacaggt aaaaatggca acaaaccacc cgaaaaactg ccgcgatcgc
     3781 gcctgataaa ttttaaccgc atgaatacct atgcaaccag atggtacaga ccacattagc
     3841 ccgtcttaat ccacttaacc tgccattttt catagtttca ccatcccagt gaaggaccat
     3901 ccagtgtgcg ttcctgtatt tccggctggc gttcccgctc cagggataac tcatgttcgc
     3961 gttcctgtat cagccgttcg tcccttatct cctgttctcg ctgtataact ggctcaagcg
     4021 ttctgtccgc tcgctcaagt gctgcacctg ctgactcaac tgcatgacct gctcgttcag
     4081 catcgcgttg tcccgttgcg taagcgaaaa cattttctgc aattccacga aggcgctctc
     4141 ccattcgttc agccgctgca tatagtcctg ttgcagttgc tctaatgcgt tcagcaaatg
     4201 tctttccagc tccgtcactc tgtgtcactc cgtcagttgt acccagtcct tcccctgata
     4261 gggaatcacc gttgccgatt tcccctgcgg gatgaccaga aatttccttc tcccgtcctg
     4321 cacaaactcc acgccccatg tcttcgcgtt cagtttctgc aatgtctctt cctgcttcct
     4381 gatttcttcc aggttcgtct gtatccgccc gccaagatac cagagcgtcc cgccactcac
     4441 ggtaaacagg aaaatgacca tccccagtaa catcatgccc gtattccctg ccagctttaa
     4501 cacgtccttc ctgtgctgca tcatcgcctc tttcacccct tcccggtgtt ttttcagtga
     4561 ttcctctgtc gaagctgtga acagggctat agcgtctttt attttcgtct cgtttgaggt
     4621 cacagccttg cttacagatt tttcgagctt gctgaactcg tcgttcagca ttgtctctgt
     4681 agattcggct ctctctttca gctttttctc gaactccgcg cccgtctgta aaagattgct
     4741 catagaatgc tcctttcagc ctgatattct tcccaccgtt cgggtctgca atgctgatac
     4801 tgcttcgcgt caccctgacc acttcaagcc ctgcctctgt gagcgcctga atcacatcct
     4861 gacggccttt tatctccccg acatgataaa gagcgtctat cccgcgtgtg acgctctctg
     4921 caagcgcctg tttcgtttcc ggcaggttat cagggagagt cagtatccgg tggttctccg
     4981 gggcgttggg gtcatgcagc ccgtaatggt gattcaccag cgtctgccag gcatcaattc
     5041 tcggcctgtc tgcccggtcg tagtacggct ggaggcgttt tccggtctgt agctccatgt
     5101 tcggaatgac aaaattcagc tcaagccgtc ccttgtcctg gtgctccacc cacaggatgc
     5161 tgtactgatt cttttcgaga ccgggcatca gtacacgctc aaagctcgcc atcacctttt
     5221 cacgtcctcc cggcggcagc tccttctccg cgaacgacag aacacctgac gtgtatttct
     5281 tcgcaaatgg cgtggcatcg atgagttccc ggacttcttc cggattaccc tgaagcaccg
     5341 ttgccccttc ccggtttcgc tcccttccca gcaggtaatc aaccggacca ctgccaccgc
     5401 cttttcccct ggcatgaaat ttaacaatca tcccgcgcgc cctgttccct gacggccaga
     5461 cgcagccgac gcagctcctc cccgatggcc atcagcgcag ccaacacctg aacccgatca
     5521 ccggaagacc actgcccgct gttcaccttc ctcgctgtct gattcaggtt attcccgatg
     5581 gcggccagct gacgcagtaa cggcggtcgc cagtgtcggc agtttcccgg aacgggcaac
     5641 cggctccccc aggcagaccc gccgcatcca taccgccagc tgtttaccct cacagcgttc
     5701 aagtaaccgg gcatgttcat catcagtaac ccgtattgtg agcatcctct cgcgtttcat
     5761 cggtatcatt accccataaa acagaaatcc cccttacacg gaggcatcag tgactaaaca
     5821 ggaaaaaacc gccattaaca tggcccgctt tatcagaagc cagacattaa cgctgctgga
     5881 gaaactcaac gaactggacg cagatgaaca ggccgatatt tgtgaatcac ttcacgacca
     5941 cgccgacgag ctttaccgca gctgcctcgc acgcttcggg gatgacggtg aaaacctctg
     6001 acacatacag ctcccggaga cggtcacagc ttgtctgtga gcggatgccg ggagcagaca
     6061 agcccgtcag ggcgcgtcag ggggtttcgg ggcgaagccc tgaaccagtc acgtagcgct
     6121 agcggagtgt atactggctt aactatgcgg catcagtgca gattgtatga aaagtgcccc
     6181 atgccgggtg tgaaatgccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     6241 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtatcag
     6301 ctcactcaaa gacggtaatg cggttatcca cagaatcagg ggataacgcc aaaggaaaca
     6361 tgtgagcaaa aaacaagaac ccggaaaagg ccacgccgct ggcgtttttc cataggctcc
     6421 gcccccctga cgagcatcac agaaatcgac gctcaagtca gaggtggcga aacccgacag
     6481 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6541 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6601 atagctcacg ctgttggtat ctcggctcgg tgtaggccgt tcgctccaag ctgggctgtg
     6661 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6721 ccaacccggt aagacacgac ttaacgccac tgtcagcagc cattggtaga gggtgtacaa
     6781 gaaattgctg atacggagtt cttgaagtga aggcccgact gcggctacac tgaaaggaca
     6841 gatttggtgg cagcggtctt gtacgccctg ttaccttggg ttagaaagcc tgagaaactt
     6901 ctaaccttcg aaaaacccac gcctgagaac gtgggttttt tcgtttacgc ccgtagagat
     6961 tacccatcat aggggtgtaa ctcaaggtct atttggatag ttttaccaag gaataatcat
     7021 ggagttccga gtaggcagcc tggcggctgc ggcttgtcat ggtctggaat taccgttata
     7081 aaaaaagata atgtcattgt ctttcaggta gttatatggc ccgttcagct aaaccccgta
     7141 aacgcaaacc cgcaccacaa agaagcaaac ttccccgcta tgttgtgaaa cttcatccgg
     7201 atgatttttt tgacgaagaa gacgctgaag ttctgcgctt tgataatttt gacgatgccg
     7261 ttgagtgctg cgctgacctg ggtattccgt tctttctgga tgcaggaaac aaaaagctgg
     7321 tcttctggtt tgttcgtgtc gatgacgaag ggtatccgga aatagcccgc tgtacggagc
     7381 gggagtttgc gaccattctt gccggtatca gtgccggtgg tatgtactgc ccggaatgcg
     7441 gcacagttca ctggccggat ggcgttaccc cacccgtctg
//
