LOCUS       CP122670                7480 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Escherichia coli strain ETEC4072 plasmid unnamed4, complete
            sequence.
ACCESSION   CP122670
VERSION     CP122670.1
DBLINK      BioProject: PRJNA917806
            BioSample: SAMN32549649
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7480)
  AUTHORS   Vereecke,N., Van Hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Virotyping and genetic antimicrobial susceptibility testing of
            porcine ETEC/STEC strains and associated plasmid types
  JOURNAL   Front Microbiol 14, 1139312 (2023)
   PUBMED   37143544
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7480)
  AUTHORS   Vereecke,N., Van hoorde,S., Sperling,D., Theuns,S., Devriendt,B. and
            Cox,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 245x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/14/2023 12:14:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,146
            CDSs (total)                      :: 5,021
            Genes (coding)                    :: 3,999
            CDSs (with protein)               :: 3,999
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 1,022
            CDSs (without protein)            :: 1,022
            Pseudo Genes (ambiguous residues) :: 0 of 1,022
            Pseudo Genes (frameshifted)       :: 859 of 1,022
            Pseudo Genes (incomplete)         :: 182 of 1,022
            Pseudo Genes (internal stop)      :: 45 of 1,022
            Pseudo Genes (multiple problems)  :: 62 of 1,022
            Pseudo Genes (short protein)      :: 4 of 1,022
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..7480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ETEC4072"
                     /isolation_source="feces"
                     /host="Sus scrofa"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="Belgium"
                     /collection_date="2021"
                     /collected_by="IMM_DI04"
     gene            join(7167..7480,1..11)
                     /locus_tag="QDW68_25185"
                     /pseudo=""
     CDS             join(7167..7480,1..11)
                     /locus_tag="QDW68_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            220..651
                     /locus_tag="QDW68_25190"
                     /pseudo=""
     CDS             220..651
                     /locus_tag="QDW68_25190"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            689..1207
                     /locus_tag="QDW68_25195"
     CDS             689..1207
                     /locus_tag="QDW68_25195"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WHH75240.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            1214..1447
                     /locus_tag="QDW68_25200"
     CDS             1214..1447
                     /locus_tag="QDW68_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WHH75241.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1810..3057)
                     /locus_tag="QDW68_25205"
     CDS             complement(1810..3057)
                     /locus_tag="QDW68_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WHH75242.1"
                     /translation="MIKFVDLFSGTGGIRLGFEQAMKEIGLSTKCVKSSEIDKKACETY
                     KLNFDEESYCDIHDVDIDESFDVLLAGFPCQAFSYAGKQLGFADTRGTLFFEVERIIKK
                     NKPKLCLLENVRGLTSHDKGRTFSTILKSLHNLGYHVEYRLLNTSNFGVPQNRVRIYII
                     ASLEKEVTLTIPNDLGAKDTHNYKPSKGGHDFYTESKVSDILEDSPDESYDCSDWFKQR
                     LEDIFEGDLNKVHGIRLIDTRNGNSLHSWDLGLKGECSNDEIIFMNLLIANRRKHIFGT
                     HQDGKALSKAQIETFFKHSETTNIIKNLIKKGYLKEKNGLYNPVCGNMSFEVFKFLDPN
                     KVSITLTASDANRLGVYHNNRLRRLTPRECARLQGYPDSYILHPKDSMAYKQLGNAVSV
                     PVIKNIFLDIMKNNFI"
                     /name="DNA cytosine methyltransferase"
     gene            complement(3054..4064)
                     /locus_tag="QDW68_25210"
     CDS             complement(3054..4064)
                     /locus_tag="QDW68_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHH75243.1"
                     /translation="MEFQELENSACLYWPKELAERAASISAISPLIETQDEFLSILTIS
                     TNKPTSCFDAVRLCNKISPNLFVKHLMVLSDIGGERLHRFFKDLDKIYPDRIMEFNIGN
                     SSYSYQFNSNRAWTTKNLNVEKSRLLQPVSDFTREMLDVCMLILWGGNTINNTNLPTEI
                     ENNCVLGNLIGNKEAIEQFVKERYIMVSRQTGGATANDLGHICEIFIKEKLYKLIDNNI
                     SLDGHHIDGVTHNDKDLTTFDIVAKNTTTKKCVGIEISFQVTTNSTIERKAGLAKSRRD
                     LLNSRGHKIAYVIDGSGNFQRSNAIKTIINHSDMTVNFSDNGINSLATFICETLK"
                     /name="hypothetical protein"
     gene            complement(4265..5452)
                     /locus_tag="QDW68_25215"
     CDS             complement(4265..5452)
                     /locus_tag="QDW68_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element IS91 family transposase"
                     /protein_id="WHH75244.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVTESVTKIMACGTTL
                     MGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVFTL
                     PCQYWSLVFHNRWLLAEMSRIAADVILEICHQTDVEPGIFTVIHTWGRDQQWHPHIHLS
                     TTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPELVIPDELAVEGNSKRDWNR
                     FLDTHYRRGWNVNISRVMDNATHVAVYFGSYLKKPPVSMSRLEHYAGQDEIGLRYNSHR
                     TKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEEVVYVITETVRKTA
                     MQIRWRGMYQRLLKVDPLKCILCGSQMRFTGLKRGYRLAELVLMHERLARQQVCG"
                     /name="IS91-like element IS91 family transposase"
     gene            complement(5452..5817)
                     /locus_tag="QDW68_25220"
     CDS             complement(5452..5817)
                     /locus_tag="QDW68_25220"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHH75238.1"
                     /translation="MARSAKPRKRKPAPQRSKLPRYVVKLHPDDFFDEEDAEVLRFDNF
                     DDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGGMY
                     CPECGTVHWPDGVTPPV"
                     /name="hypothetical protein"
     gene            complement(6911..7102)
                     /locus_tag="QDW68_25225"
     CDS             complement(6911..7102)
                     /locus_tag="QDW68_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WHH75239.1"
                     /translation="MTKQEKTAINMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          6749..6833
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          complement(6181..6738)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.583"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1551
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.649"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7138..7304
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.214"
                     /coverage="97.0"
                     /evalue="4.19e-50"
     Typing          7307..7479
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="97.0"
                     /evalue="4.19e-50"
ORIGIN
        1 atgattgtta aatttcatgc caggggaaaa ggcggtggca gtggtccggt tgattacctg
       61 ctgggaaggg agcgtaaccg ggaaggggca acggtgcttc agggtaatcc ggaagaagtc
      121 cgggaactca tcgatgccac gccatttgcg aagaaataca cgtcaggtgt tctgtcgttc
      181 gcggagaagg agctgccgcc gggaggacgt gaaaaggtga tggcgagctt tgagcgtgta
      241 ctgatgcccg gtctcgaaaa gaatcagtac agcatcctgt gggtggagca ccaggacaag
      301 ggacggcttg agctgaattt tgtcattccg aacatggagc tacagaccgg aaaacgcctc
      361 cagccgtact acgaccgggc agacaggccg agaattgatg cctggcagac gctggtgaat
      421 caccattacg ggctgcatga ccccaacgcc ccggagaacc accggatact gactctccct
      481 gataacctgc cggaaacgaa acaggcgctt gcagagagcg tcacacgcgg gatagacgct
      541 ctttatcatg tcggggagat aaaaggccgt caggatgtga ttcaggcgct cacagaggca
      601 gggcttgaag tggtcagggt gacgcgaagc agtatcagca ttgcagaccc gaacggtggg
      661 aagaatatca ggctgaaagg agcattctat gagcaatctt ttacagacgg gcgcggagtt
      721 cgagaaaaag ctgaaagaga gagccgaatc tacagagaca atgctgaacg acgagttcag
      781 caagctcgaa aaatctgtaa gcaaggctgt gacctcaaac gagacgaaaa taaaagacgc
      841 tatagccctg ttcacagctt cgacagagga atcactgaaa aaacaccggg aaggggtgaa
      901 agaggcgatg atgcagcaca ggaaggacgt gttaaagctg gcagggaata cgggcatgat
      961 gttactgggg atggtcattt tcctgtttac cgtgagtggc gggacgctct ggtatcttgg
     1021 cgggcggata cagacgaacc tggaagaaat caggaagcag gaagagacat tgcagaaact
     1081 gaacgcgaag acatggggcg tggagtttgt gcaggacggg agaaggaaat ttctggtcat
     1141 cccgcagggg aaatcggcaa cggtgattcc ctatcagggg aaggactggg tacaactgac
     1201 ggagtgacac agagtgacgg agctggaaag acatttgctg aacgcattag agcaactgca
     1261 acaggactat atgcagcggc tgaacgaatg ggagagcgcc ttcgtggaat tgcagaaaat
     1321 gttttcgctt acgcaacggg acaacgcgat gctgaacgag caggtcatgc agttgagtca
     1381 gcaggtgcag cacttgagcg agcggacaga acgcttgagc cagttataca gcgagaacag
     1441 gagataaggg acgaacggct gatacaggaa cgcgaacatg agttatccct ggagcgggaa
     1501 cgccagccgg aaatacagga acgcacactg gatggtcctt cactgggatg gtgaaactat
     1561 gaaaaatggc aggttaagtg gattaagacg ggctaatgtg gtctgtacca tctggttgca
     1621 taggtattca tgcggttaaa atttatcagg cgcgatcgcg gcagtttttc gggtggtttg
     1681 ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc ggtgcgcgta
     1741 atgcgacgtt atggtaaatc tgttaaattt aattaaataa ccatagagca gttttgctag
     1801 taatatggtt tatataaagt tattcttcat tatatcaaga aaaatatttt taatgactgg
     1861 tacgcttaca gcattcccta attgtttata tgccatgcta tcttttgggt gtaagatata
     1921 tgagtctggg taaccttgta gtcttgcgca ttctcttggc gtaaggcgtc gcagtctatt
     1981 gttgtgatag actccaagtc tattggcatc agatgccgta agtgtaattg acactttatt
     2041 tgggtcaagg aatttgaata cttcaaaaga catatttccg caaactgggt tgtatagtcc
     2101 gtttttctct ttaaggtaac cctttttaat taggtttttt attatgttgg tggtttcgct
     2161 atgtttaaaa aatgtttcaa tttgtgcttt tgacaaagcc ttgccgtctt ggtgtgtgcc
     2221 aaaaatatgc tttctacggt ttgcaattaa taaattcatg aaaattattt catcatttga
     2281 gcactcgcct tttagcccaa gatcccaaga gtgcaaggag tttccgttcc ttgtgtcaat
     2341 tagcctgatt ccatgaactt tatttaggtc accttcaaaa atgtcttcaa ggcgttgctt
     2401 gaaccaatcg ctgcaatcat atgactcgtc tggactatcc tctagtatgt cagaaacttt
     2461 tgattctgta taaaaatcat gaccaccttt ggatggtttg taattgtgcg tatctttggc
     2521 tccaagatca ttaggaatag taagtgttac ctctttttct aatgaggcta ttatatatat
     2581 tctaacccta ttttgaggga caccaaaatt acttgtgttt aatagtctgt attcaacatg
     2641 ataccctaaa ttatgaagag attttaatat tgttgaaaat gttcttcctt tatcatgtga
     2701 agttaatcca cgcacattct ccaaaagaca taacttaggc ttgtttttct ttatgattct
     2761 ttctacttca aaaaataatg tgcctcgggt gtctgcaaaa ccaagttgtt ttccagcata
     2821 agagaacgcc tgacaaggaa atcctgccaa taaaacatcg aagctttcat ctatatcaac
     2881 atcatgtata tcacaatatg attcttcgtc gaagttaagt ttgtatgttt cgcatgcttt
     2941 tttgtcaatt tctgagcttt ttacgcattt tgttgacaat ccaatttctt tcatggcttg
     3001 ctcaaaacca agtctaatac cacctgtgcc agaaaaaagg tcaacaaatt taatcatttc
     3061 aatgtctcgc agatgaatgt tgctagtgaa tttataccat tatctgaaaa gttaacggtc
     3121 atgtcactat ggttaattat tgttttgatg gcatttgaac gctgaaaatt accagaccca
     3181 tcaatcacat atgctatttt atgtcctctt gaattgagca aatctcttct gcttttggct
     3241 aatcctgctt ttctttctat tgttgagttt gttgtaactt gaaaacttat ttcaatacca
     3301 acacattttt ttgttgttgt gttttttgct acaatatcaa aagtcgtaag atctttatcg
     3361 ttatgagtta caccatcgat atggtgtcca tctagagata tattgttatc aataagcttg
     3421 taaagttttt cttttataaa gatctcgcat atgtgtccaa gatcatttgc cgttgcacct
     3481 cctgtttgcc tgctaaccat tatatatctt tcttttacaa actgttctat tgcttctttg
     3541 tttccaatta aattccccaa aacacagtta ttttctattt cggttggaag atttgtattg
     3601 tttatagtat tacctcccca taggatcagc atgcaaacat caagcatttc acgtgtaaaa
     3661 tcacttacag gttgcaagag ccgtgatttt tccacgttta aattcttggt tgtccatgct
     3721 ctattgctat tgaattggta agagtaagag ctatttccta tattgaactc cattattctg
     3781 tcggggtaga tcttatctaa atctttaaag aagcgatgaa gtctttcgcc tccaatatct
     3841 gacaaaacca ttaagtgctt gacaaatagg ttgggtgata tcttattgca taaccgtacg
     3901 gcatcaaaac aagatgtagg cttatttgtt gatattgtta gtattgataa aaattcatct
     3961 tgggtctcta taagagggct tatggcgcta atagatgctg ctctttctgc aagttctttg
     4021 ggccaatata agcatgcgct attttctaat tcttggaact ccatgattat tccttgatcg
     4081 ataggaattt aaatccccaa aagactaaaa aagcaccaca aaacggatgt ttcttcaaca
     4141 ccccttgtgc accatatgag cggaaacgac tattaaacag gatgacactg attgatacag
     4201 ggtatgaatg gcggtttttt gctccgtttc cggtaaaatg gacgcaactt cccctctgcg
     4261 gctctcagcc gcacacctgc tgtcgtgcca gtcgctcatg catcaggacc agctctgcca
     4321 gtcggtaacc ccgcttcagc cccgtaaaac gcatctgact tccgcacaga atgcacttca
     4381 gcgggtcaac cttcagtaac ctctgataca tccctctcca cctgatttgc atcgccgttt
     4441 ttctcaccgt ctccgttatg acgtacacaa cttcttccag taagcggcgc ttcaccggac
     4501 tcaggaaacc gtagtacctc accatacgga accccttatc tgctacatgc caggagaacc
     4561 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgta cggtgactgt
     4621 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagccgactc atcgacaccg
     4681 gtggcttttt caggtaagag ccgaagtaca ccgccacatg tgtggcgtta tccatcaccc
     4741 tggatatgtt gacattccag ccgcggcggt aatgcgtgtc caggaagcga ttccagtccc
     4801 gtttgctgtt tccttccact gccagttcat ccggtattac cagctccggg tatttccggg
     4861 acagtagccg cgttatccgg taacgccaca tgctcatcac cttacgggcg taaaaatgaa
     4921 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
     4981 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
     5041 ctgtctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgccagta
     5101 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
     5161 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
     5221 ccccgcagtg cggacaggag cggcttttac accggaagca gacctttttg gtgtggcaac
     5281 agtccggtga cgaacagcac cattgcgtgt agcccatcag cgtggtcccg catgccatga
     5341 tttttgtcac tgactcagtc accaccggac gcactgaacc ttccggctgt ttctccagcc
     5401 agttaagcca gcggttaccc tgctgaaaaa tatcggcaaa acggggaagc atcagacggg
     5461 tggggtaacg ccatccggcc agtgaactgt gccgcattcc gggcagtaca taccaccggc
     5521 actgataccg gcaagaatgg ttgcaaactc ccgctccgta cagcgggcta tttccggata
     5581 cccttcgtca tcgacacgaa caaaccagaa gaccagcttt ttgtttcctg catccagaaa
     5641 gaacggaata cccaggtcag cgcagcactc aacggcatcg tcaaaattat caaagcgcag
     5701 aacttcagcg tcttcttcgt caaaaaaatc atccggatga agtttcacaa catagcgggg
     5761 aagtttgctt ctttgtggtg cgggtttgcg tttacggggt ttagctgaac gggccatata
     5821 actacctgaa agacaatgac attatctttt tttataacgg taattccaga ccatgacaag
     5881 ccgcagccgc caggctgcct actcggtaaa actatccaaa tagaccttga gttacacccc
     5941 tatgatgggt aatctctacg ggcgtaaacg aaaaaaccca cgttctcagg cgtgggtttt
     6001 tcgaaggtta gaagtttctc aggctttcta acccaaggta acagggcgta caagaccgct
     6061 gccaccaaat ctgtcctttc agtgtagccg cagtcgggcc ttcacttcaa gaactccgta
     6121 tcagcaattt cttgtacacc ctctaccaat ggctgctgac agtggcgtta agtcgtgtct
     6181 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6241 gggttcgtgc acacagccca gcttggagcg aacggcctac accgagccga gataccaaca
     6301 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6361 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6421 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttc tgtgatgctc
     6481 gtcagggggg cggagcctat ggaaaaacgc cagcggcgtg gccttttccg ggttcttgtt
     6541 ttttgctcac atgtttcctt tggcgttatc ccctgattct gtggataacc gcattaccgt
     6601 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag
     6661 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggcatttc
     6721 acacccggca tggggcactt ttcatacaat ctgcactgat gccgcatagt taagccagta
     6781 tacactccgc tagcgctacg tgactggttc agggcttcgc cccgaaaccc cctgacgcgc
     6841 cctgacgggc ttgtctgctc ccggcatccg ctcacagaca agctgtgacc gtctccggga
     6901 gctgtatgtg tcagaggttt tcaccgtcat ccccgaagcg tgcgaggcag ctgcggtaaa
     6961 gctcgtcggc gtggtcgtga agtgattcac aaatatcggc ctgttcatct gcgtccagtt
     7021 cgttgagttt ctccagcagc gttaatgtct ggcttctgat aaagcgggcc atgttaatgg
     7081 cggttttttc ctgtttagtc actgatgcct ccgtgtaagg gggatttctg ttttatgggg
     7141 taatgatacc gatgaaacgc gagaggatgc tcacaatacg ggttactgat gatgaacatg
     7201 cccggttact tgaacgctgt gagggtaaac agctggcggt atggatgcgg cgggtctgcc
     7261 tgggggagcc ggttgcccgt tccgggaaac tgccgacact ggcgaccgcc gttactgcgt
     7321 cagctggccg ccatcgggaa taacctgaat cagacagcga ggaaggtgaa cagcgggcag
     7381 tggtcttccg gtgatcgggt tcaggtgttg gctgcgctga tggccatcgg ggaggagctg
     7441 cgtcggctgc gtctggccgt cagggaacag ggcgcgcggg
//
