LOCUS       CP118827                4439 bp    DNA     linear   BCT 15-MAY-2023
DEFINITION  Staphylococcus hominis strain 7056 plasmid pML7056-2.
ACCESSION   CP118827
VERSION     CP118827.1
DBLINK      BioProject: PRJNA937407
            BioSample: SAMN33407035
KEYWORDS    .
SOURCE      Staphylococcus hominis
  ORGANISM  Staphylococcus hominis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Belhout,C., Boyen,F., Vereecke,N., Theuns,S., Taibi,N., Stegger,M.,
            de la Fe-Rodriguez,P.Y., Bouayad,L., Elgroud,R. and Butaye,P.
  TITLE     Prevalence and Molecular Characterization of Methicillin-Resistant
            Staphylococci (MRS) and Mammaliicocci (MRM) in Dromedary Camels from
            Algeria: First Detection of SCCmec-mecC Hybrid in
            Methicillin-Resistant Mammaliicoccus lentus
  JOURNAL   Antibiotics (Basel) 12 (4), 674 (2023)
   PUBMED   37107036
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Belhout,C., Stegger,M., Vereecke,N., Elgroud,R. and Butaye,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 270x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/27/2023 12:51:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,264
            CDSs (total)                      :: 2,180
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 165 of 198
            Pseudo Genes (incomplete)         :: 33 of 198
            Pseudo Genes (internal stop)      :: 11 of 198
            Pseudo Genes (multiple problems)  :: 11 of 198
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus hominis"
                     /mol_type="genomic DNA"
                     /strain="7056"
                     /isolation_source="Nose"
                     /host="Camelus dromedarius"
                     /db_xref="taxon:1290"
                     /plasmid="pML7056-2"
                     /geo_loc_name="Algeria: Ouargla"
                     /collection_date="2022-06-06"
     gene            <1..1380
                     /gene="tet(K)"
                     /locus_tag="PYH62_11300"
     ARG             2..1377
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1566..2807
                     /gene="mobV"
                     /locus_tag="PYH62_11305"
     CDS             1566..2807
                     /gene="mobV"
                     /locus_tag="PYH62_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_process:
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WHI80298.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            3334..4278
                     /locus_tag="PYH62_11310"
     CDS             3334..4278
                     /locus_tag="PYH62_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0003677 - DNA binding [Evidence IEA]; GO_function:
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WHI80299.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     Typing          3335..4278
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1567..2804
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgtttagtt tatataaaaa atttaaaggt ttgttttata gcgttttatt ttggctttgt
       61 attctttcat tttttagtgt attaaatgaa atggttttaa atgtttcttt acctgatatt
      121 gcaaatcatt ttaatactac tcctggaatt acaaactggg taaacactgc atatatgtta
      181 actttttcga taggaacagc agtatatgga aaattatctg attatataaa tataaaaaaa
      241 ttgttaatta ttggtattag tttgagctgt cttggttcat tgattgcttt tattggtcac
      301 aatcactttt ttattttgat ttttggtagg ttagtacaag gagtaggatc tgctgcattc
      361 ccttcactga ttatggtggt tgtagctaga aatattacaa gaaaaaaaca aggcaaagcc
      421 tttggtttta taggatcaat tgtagcttta ggtgaagggt taggtccttc aataggggga
      481 ataatagcac attatattca ttggtcttac ctacttatac ttcctatgat tacaatagta
      541 actatacctt ttcttattaa agtaatggta cctggtaaat caacaaaaaa tacattagat
      601 atcgtaggta ttgttttaat gtctataagt attatatgtt ttatgttatt tacgacaaat
      661 tataattgga cttttttaat actcttcaca atcttttttg tgatttttat taaacatatt
      721 tcaagagttt ctaacccttt tattaatcct aaactaggga aaaacattcc gtttatgctt
      781 ggtttgtttt ctggtgggct aatattttct atagtagctg gttttatatc aatggtgcct
      841 tatatgatga aaactattta tcatgtaaat gtagcgacaa taggtaatag tgttattttt
      901 cctggaacca tgagtgttat tgtttttggt tattttggtg gttttttagt ggatagaaaa
      961 ggatcattat ttgtttttat tttaggatca ttgtctatct ctataagttt tttaactatt
     1021 gcattttttg ttgagtttag tatgtggttg actactttta tgtttatatt tgttatgggc
     1081 ggattatctt ttactaaaac agttatatca aaaatagtat caagtagtct ttctgaagaa
     1141 gaagttgctt ctggaatgag tttgctaaat ttcacaagtt ttttatcaga gggaacaggt
     1201 atagcaattg taggaggttt attgtcacta caattgatta atcgtaaact agttctggaa
     1261 tttataaatt attcttctgg agtgtatagt aatattcttg tagccatggc tatccttatt
     1321 attttatgtt gtcttttgac gattattgta tttaaacgtt ctgaaaagca gtttgaatag
     1381 ttatattata ttttggttta gaactatgag tggctagcat tttgccactc attttttgcg
     1441 ttagcaaaaa caggtttaag cctcgcagag cacacgtatt aacgacttat taaaaataag
     1501 tctagtgtgt tagacttaaa ctattaaata cacatgaaac ctttgtgctt aggagtgatt
     1561 tttatatgtc ttattccatt gttagagttt caaaagttaa atctggaaca aatacaacgg
     1621 gcatacaaaa acatgttcaa agagaaaata ataattatga aaatgaagat atagaccata
     1681 gtaaaactta cttaaattat gatttggtaa atgctaataa acagaatttt aataacttga
     1741 ttgatgaaaa aatcgaacag aattatacag gcaaaagaaa aattagaaca gacgcgatta
     1801 aacacattga tggtttaatt acatcagaca atgatttctt tgataatcaa acgccagaag
     1861 atacaaagca gttttttgaa tatgctaaag agtttttaga acaagaatac ggtaaagata
     1921 atttattata tgcaacagtt cacatggacg aaaaaacacc acatatgcat tatggcgttg
     1981 ttccaataac tgatgatggt cgtttaagtg ctaaagaagt tgtaggtaat aaaaaagctt
     2041 taacagcgtt tcaagataga tttaatgagc atgttaaaca acgaggatat gatttagaac
     2101 gtgggcaatc aagacaagta acaaatgcta aacatgagca aataagtcag tataaacaaa
     2161 aaacagaata tcataagcaa gaatatgaac gtgagagcca aaaaacagac catataaagc
     2221 aaaagaacga taaattaatg caagagtacc aaaaatcgtt aaatacgctt aaaaagccta
     2281 taaatgttcc gtatgagcaa gaaactgaaa aagtaggtgg tttatttagc aaagaaatac
     2341 aagaaactgg aaatgttgta ataagccaaa aagatttcaa tgaatttcag aaacagataa
     2401 aagctgctca agatatttcg gaagattacg agtatataaa gtctggtaga gccttagatg
     2461 ataaagataa ggaaatacga gagaaagatg atttattaaa taaagcagtt gagcgtattg
     2521 aaaacgcaga cgataatttt aaccaacttt acgaaaatgc aaagccactt aaagagaata
     2581 tagaaatagc gttaaagctt ttaaaaatct tactaaaaga gttagaacga gttttaggaa
     2641 gaaatacctt tgcggaaaga gttaataagt taacagaaga tgaaccaaaa ctaaatggtt
     2701 tagcaggaaa cttagataaa aaaatgaatc cagaattata ttcagaacag gaacagcaac
     2761 aagaacaaca aaagaatcaa aaacgagata gaggtatgca cttatagaac atgcatttat
     2821 gccgagaaaa cttattggtt ggaatgggct atgtgttagc taacttgtta gcgagttggt
     2881 tggacttgaa ttgggattaa tcccaagaaa gtaccaactc aacaacacat aaagccctgt
     2941 aggttccgac caataaggaa attggaataa agcaataaaa ggagttgaag aaatgaaatt
     3001 cagagaagcc tttgagaatt ttataacaag taagtatgta cttggtgttt tagtagtttt
     3061 aactgtttac cagataatac aaatgcttaa ataaaaaaag acttgatctg attagaccaa
     3121 gtcttttgat agtgttatat taataacaaa ataaaaagga gtcgctcacg ccctgaccaa
     3181 agtttgtgaa cgacatcatt caaagaaaaa aacactgagt tgtttttata atcttgtata
     3241 tttagatatt aaacgatatt taaatataca tcaagatata tatttgggtg agcgattcct
     3301 taaacgaaat tgagattaag gagtcgattt tttatgtata aaaacaatca tgcaaatcat
     3361 tcaaatcatt tggaaaatca cgatttagac aatttttcta aaaccggcta ctctaatagc
     3421 cggttggacg cacatactgt gtgcatatct gatccaaaat taagttttga tgcaatgacg
     3481 atcgttggaa atctcaaccg agacaacgct caagcccttt ctaaatttat gagtgtagag
     3541 ccccaaataa gactttggga tattcttcaa acaaagttta aagctaaagc acttcaagaa
     3601 aaagtttata ttgaatatga caaagtgaaa gcagatagtt gggatagacg taatatgcgt
     3661 attgaattta atccaaacaa acttacacga gatgaaatga tttggttaaa acaaaatata
     3721 ataagctaca tggaagatga cggttttaca agattagatt tagcctttga ttttgaagat
     3781 gatttgagtg actactatgc aatgtctgat aaagcagtta agaaaactat tttttatggt
     3841 cgtaatggta agccagaaac aaaatatttt ggcgtgagag atagtaatag atttattaga
     3901 atttataata aaaagcaaga acgtaaagat aatgcagatg ctgaagttat gtctgaacat
     3961 ttatggcgtg tagaaatcga acttaaaaga gatatggtgg attactggaa tgattgcttt
     4021 agtgatttac atatcttgca accagattgg aaaactatcc aacgcactgc ggatagagca
     4081 atagttttta tgttattgag tgatgaagaa gaatggggaa agcttcacag aaattctaga
     4141 acaaaatata agaatttgat aaaagaaatt tcgccagtcg atttaacgga cttaatgaaa
     4201 tcgactttaa aagcgaacga aaaacaattg caaaaacaaa tcgatttttg gcaacatgaa
     4261 tttaaatttt ggaaatagtg tacatattaa tattactgaa caaaaatgat atatttaaac
     4321 tattctaatt taggaggatt tttttatgaa gtgtctattt aaaaatttgg ggaatttata
     4381 tgaggtgaaa gaataattta cccctataaa ctttagtcac ctcaagtaaa gaggtaaaa
//
