LOCUS       CP118826               13607 bp    DNA     circular BCT 15-MAY-2023
DEFINITION  Staphylococcus hominis strain 7056 plasmid pML7056-1, complete
            sequence.
ACCESSION   CP118826
VERSION     CP118826.1
DBLINK      BioProject: PRJNA937407
            BioSample: SAMN33407035
KEYWORDS    .
SOURCE      Staphylococcus hominis
  ORGANISM  Staphylococcus hominis
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13607)
  AUTHORS   Belhout,C., Boyen,F., Vereecke,N., Theuns,S., Taibi,N., Stegger,M.,
            de la Fe-Rodriguez,P.Y., Bouayad,L., Elgroud,R. and Butaye,P.
  TITLE     Prevalence and Molecular Characterization of Methicillin-Resistant
            Staphylococci (MRS) and Mammaliicocci (MRM) in Dromedary Camels from
            Algeria: First Detection of SCCmec-mecC Hybrid in
            Methicillin-Resistant Mammaliicoccus lentus
  JOURNAL   Antibiotics (Basel) 12 (4), 674 (2023)
   PUBMED   37107036
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13607)
  AUTHORS   Belhout,C., Stegger,M., Vereecke,N., Elgroud,R. and Butaye,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) Department of Pathobiology, Pharmacology and
            Zoological Medicine, Universiteit Gent, Salisburylaan 133, Merelbeke
            9820, Belgium
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Trycycler; minimap2; medaka v. v0.5.3; v2.20;
            v1.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 270x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/27/2023 12:51:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,264
            CDSs (total)                      :: 2,180
            Genes (coding)                    :: 1,982
            CDSs (with protein)               :: 1,982
            Genes (RNA)                       :: 84
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 165 of 198
            Pseudo Genes (incomplete)         :: 33 of 198
            Pseudo Genes (internal stop)      :: 11 of 198
            Pseudo Genes (multiple problems)  :: 11 of 198
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13607
                     /organism="Staphylococcus hominis"
                     /mol_type="genomic DNA"
                     /strain="7056"
                     /isolation_source="Nose"
                     /host="Camelus dromedarius"
                     /db_xref="taxon:1290"
                     /plasmid="pML7056-1"
                     /geo_loc_name="Algeria: Ouargla"
                     /collection_date="2022-06-06"
     gene            join(13587..13607,1..1725)
                     /locus_tag="PYH62_11230"
     CDS             join(13587..13607,1..1725)
                     /locus_tag="PYH62_11230"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="WHI80290.1"
                     /translation="MKKNKGKMEANQIITNTIKNKQAEDYDGNFDELLFDTFFYDYNNI
                     ELSRIKTDDYGDGGADYIFFVCNDQYILSSEDVDQLEEDSEVEIHFIQIKETDKLDSAV
                     PTRLIELTRNFFKYEKAPHYNEEIISNIELFQKITEKILPKGRIDVTFYYFGLFSKHQL
                     DNAKDIKQRFETLKNSIADYDFVKNSNYKIYTSSDIFNSLSKEREYEYQFKKIDKYSAE
                     VNEETDETEALISLIPIQQFYDFITDDDDTINTKLFEHNIRDYKGKSNVNKDIINTLDK
                     KESLQFWWLNNGITIIVEEIKESSSAKKISIKNPQIVNGLQTSYSIFNYFHENSDKLKK
                     ENRSIFIKIIRIDPEEEGVELDITKATNKQNEIRDKDIRANDEVQKNIELYFKTKGKFY
                     QRKDKYYTNRRMNKKDIITLFDLAKYVYTIMFKDPVFTRNNPGKLLKDDKYSKIFKIDD
                     PKQDYDIYYCCYKIYDTINAYNKGKINIVSDEFSKSNFIHHIVYICVSILQNSKDYDPS
                     NIKNINLNNVNEELIDIAYKILNKVIVNNNIPGSQVLKNIKEQPFLALMRKEIDKYNKS
                     KKNKK"
                     /name="AIPR family protein"
     gene            1904..2512
                     /locus_tag="PYH62_11235"
     CDS             1904..2512
                     /locus_tag="PYH62_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WHI80291.1"
                     /translation="MIIGYARVSSIDQNLERQLDNLKTFGVEKIFTEKQSGKSVENRHV
                     FQEALNFVRMGDRFVVESIDRLGRNYDEVINTVNYLKDKEVQLMITSLPMMNEVIGNPL
                     LDKFMKDLIIQILAMVSEQERNESKRRQAQGIQVAKEKGVYKGRPLLYSANAKDPQKRI
                     IYHRVVEMLEEGQAISKIAKEVNITRQTVYRIKHDKGLS"
                     /name="recombinase family protein"
     gene            2695..2922
                     /locus_tag="PYH62_11240"
     CDS             2695..2922
                     /locus_tag="PYH62_11240"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHI80292.1"
                     /translation="MVGNDFERKKDELNGIIKYFSEDNDNDGWHESEKSTALGTIALRL
                     EEIGEETIEYNTEERKELLLLSAKAIRLIY"
                     /name="hypothetical protein"
     gene            complement(3006..3569)
                     /locus_tag="PYH62_11245"
     CDS             complement(3006..3569)
                     /locus_tag="PYH62_11245"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="WHI80293.1"
                     /translation="MVRATPLLTTTKPRKIHKDGIYFKGLRYFSTNLIAYITEEVFIRY
                     DPQDISEIRVYLNNQFLSIAYCSDLENQSISIKEIERLRVNKKRELGKIGYRSLKKQEE
                     FNGLEELVRLQPRNYEIDLIIIDEVDRLNYKTLETIRDIYDQLDIGVVLIGMPGTEKRL
                     SRYPQLYSRIGFSHQFEKLSNVHY"
                     /name="Mu transposase C-terminal domain-containing protein"
     gene            complement(3581..4888)
                     /locus_tag="PYH62_11250"
     CDS             complement(3581..4888)
                     /locus_tag="PYH62_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor"
                     /protein_id="WHI80294.1"
                     /translation="MTDKKKLDTKNDKAWQILFETHDIVDECHKNGFYEISADDIKKVR
                     EPRLMTKFDHYNNLPIQFKDENLVILPNRRGNYVIGNFDVYKELKYDETIKPEIVSFPH
                     TIDTIKPQIVTSESVALNIAHISRMLSKLANNENLDLTVTGRMGSGKFSYDVLNKTTNT
                     NYNFAVENSQIEIDGSYESKNHFLIVESKMHTAEDFMARQLYYPFRTLSSRTNKKINPI
                     FFTLSNDVYSFFIYDVVDINNYNSLQLVKQVNFMFNHKSIIKDDLSNLYKNANEVPEPL
                     APFPQADSFERVIDLLNLLVEKDLMFEEITLNYDFTSRQTGYYTNAGIYLGLIEKFTDE
                     DNEIAYRITKKGLDIMQKEIKEKYLSLATEIISHKPFYLAFKHYIKNNQVPSKKETVNY
                     MKKCNIYNVGSDVTRDRRSSTIRGWIKWIANLPEEI"
                     /name="translation elongation factor"
     gene            5034..5870
                     /locus_tag="PYH62_11255"
     CDS             5034..5870
                     /locus_tag="PYH62_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="WHI80295.1"
                     /translation="MTKNNEIIKPILKYVGGKSKVLPDIKANMPSEYNRYFEPFLGGAS
                     VLLGIQPKSAIVNDINFEVINLYRVVKNNPYELIEDLKKHVNEKEYYYSIRALDRDEEL
                     FAKLTAVERASRILYINKTGFNGILRYSQKGYLNVPMGKYKNPNIVNEEGILKLSKYLN
                     ENDITILNTDFIEACADAKEGDFIYSDSPYIPISKTSAFTSYNADGFTIEDQIRLRDLA
                     LDLDKRGCKVMLSNSSADMVYDLYRDFNIRHVEVARAINSKGDGRGKIKEVLITNY"
                     /name="DNA adenine methylase"
     gene            complement(5990..6751)
                     /locus_tag="PYH62_11260"
     CDS             complement(5990..6751)
                     /locus_tag="PYH62_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WHI80296.1"
                     /translation="MKTIKMIADELGVTKQTIVNNAKRLNISFEKENGINYIKDNDYLK
                     IIEKITKKERTTHNKESVKKELFNENTTKVEKYIHNNSNSFETLKTKVNELEKQIEIFE
                     TRAKNDEKYIENLTKQLDQQNSNVNTLNKLLENQQILSLENNKKIQKLERQLEEERQLN
                     YSFDTTMNNRQNINPQEAIFTEESQNINQQQKECQPTEVNHKNITEENKHDSTDELTKE
                     DKFVEKTKSNSSDNREAEPPKKRFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            7193..8128
                     /locus_tag="PYH62_11265"
     CDS             7193..8128
                     /locus_tag="PYH62_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WHI80283.1"
                     /translation="MPNFEKYNLSQVKTERFYQLPKYLFEDAYFKKMSAEAKIMYALLK
                     DRFELSLQNEWVDKNNNIYFIFSNKHLCEYLGYAEQKIIKLKKELIKFNLLTQERVGLN
                     KPNRLYLLKPNYDIEASHIKELPNSQFQNNEFGSSRTVNLSGQELPNSQSNDTDYNDTD
                     YIKTNSNDTYDMNDKKTTYSSNHTNHSNHYYSKFNDEALKFQLLEELPQSIQTYLSNFS
                     VSEIKLIKSVLLKAKTSFNNSIDAYYLLEDMEFEIVSVLKRFKATLIQKNETVEAMQGY
                     LMKSLKSEFAEVHTLNKRRDHLPITSLFDQ"
                     /name="replication initiator protein A"
     gene            complement(8354..8593)
                     /locus_tag="PYH62_11270"
     CDS             complement(8354..8593)
                     /locus_tag="PYH62_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHI80284.1"
                     /translation="MNDFKNLKKTNAAIEKAELRKHRLKNLDRKERAHRLIRKGAILEK
                     YFECEHLSPNETEELLKMYAKYINTNKPNKFKKK"
                     /name="hypothetical protein"
     gene            complement(8598..8879)
                     /locus_tag="PYH62_11275"
     CDS             complement(8598..8879)
                     /locus_tag="PYH62_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHI80285.1"
                     /translation="MSKIKQTDITKLSQLEQQIEQLKEQKKQQEAQLSHKIGGYFLNKL
                     DLNMINSSDELYKIMDKVIEVFNQDIEQNGSNRNNENLKENEINQNSK"
                     /name="hypothetical protein"
     gene            9083..11005
                     /locus_tag="PYH62_11280"
     CDS             9083..11005
                     /locus_tag="PYH62_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WHI80286.1"
                     /translation="MEKGYFMLRVTNINRATKNIVASASYRSDEALYSERTDEKIKFRN
                     HIVKPESMILTPQNAPDWTKDRQRLWNEVDKVEKHNAKTKNPRLAKEVLLSLPNDFDRE
                     VQTELTKDFIKSEFVDKGMVADISIHRDDMNNPHAHVLLTQRPFNSDGTWGKKTKTRTQ
                     YDENGDAILNKTGNKVRKQERFADFDFKEVRKHWELKLNQYSEREQSLRRYDSRSFEAQ
                     GLEKIAEIPLTREEYRLEQNEKKRCEKEGIEYKPVTYYGQKNEQIRRYNRGEVSQLFSD
                     KEKEQAQNEIRLFIQNANDQVSENEQSYSILFDRYQRDVGYSEAKETIKNTFDSASTFG
                     RKIQNDLLKNEIKRRQLKFYAKNYDENYQFRKEIESKGYSQQEFVNIVETNYKALLNEK
                     NKLASREEKRKEIYQSAKDVYLTESKKNNLIVQQMYPKSHKQFSSDEKAFIVNEAQKGN
                     IIKASNVRYLFKQNNNIPTNVLLEDTYKKASKDIFFSERQLKHLEKDSFEYNVELQFIK
                     EKEKEIESYKEHIDEDLRIDIGDKNFEKVKGQTSNEKVQLLIKLEKYNNVDQEEVIEKH
                     INQVDRNDNDNRIEHGNEQSNHINLPTINASDFLMYYANETEKQNNINYKKKKKTQERN
                     KQLRR"
                     /name="MobA/MobL family protein"
     gene            11313..11771
                     /locus_tag="PYH62_11285"
     CDS             11313..11771
                     /locus_tag="PYH62_11285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WHI80287.1"
                     /translation="MDPIVSNILTSLAVNYFSSFSEAIVKEFFKKAIKEKPEIEDELKA
                     AKSSYDFEEIFQESTGIIALNANDDEIKVFGGLLDALRGIKFDHGNGKVEIQGSVLNAP
                     VLVTGGTVKSSGSTFIGENTELKSSGSRILVGKDSGIYLGGNSSISQN"
                     /name="hypothetical protein"
     gene            11786..12400
                     /locus_tag="PYH62_11290"
     CDS             11786..12400
                     /locus_tag="PYH62_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WHI80288.1"
                     /translation="MKYKVINNINRTAKKSRNKLFFKDKDIKNVRFFNSNLTYFHFKKR
                     VFKNVDFLGCNLKHSVFHGGIFINCVFYNTNLSKSNFLGANFVNCHFINCKFIKTKNFN
                     FNNCNVITSDFSSVNVSKKLENKIFTLNRVPAFKRSYIFTTKRSKGTKINKGIIAVLKN
                     EFSDHELFRILSVVNNKTNARIKNFITYGQFYEFGRIYFKK"
                     /name="pentapeptide repeat-containing protein"
     gene            complement(12747..13295)
                     /locus_tag="PYH62_11295"
     CDS             complement(12747..13295)
                     /locus_tag="PYH62_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WHI80289.1"
                     /translation="MSKIGYIRHLPKSESIDLQLLKLRSYGCNCIFIESEDKNIRELDE
                     LSNCLKFLKKGDTLVVNNLQHLGKTPKEFVLFSQWLLTKNIKLDIVDMKISTSDEKTKD
                     MFYSILYAINENESTLASERTKLGLKTARARGRNGGRPSISNEIKKQIKDLYREKKLTV
                     VEIAKKVGVSTTTVYRTLK"
                     /name="recombinase family protein"
     Typing          7194..8128
                     /name="rep_cluster_1215"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.397"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9084..10936
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.689"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 atggaagcaa atcaaattat tacaaatact ataaaaaata aacaggctga agattacgat
       61 ggtaattttg atgaattact atttgataca tttttttatg attataataa tatagaacta
      121 agccgaatta aaacagatga ctatggagat ggaggagctg actatatatt ttttgtatgt
      181 aatgatcaat atatacttag ttcagaagat gtagaccaat tagaggaaga ttcagaagtt
      241 gaaatacact ttatacaaat taaagaaact gataaattgg actctgctgt acctacaaga
      301 ttaattgaat tgacaagaaa cttttttaaa tatgaaaaag ctcctcacta taatgaagaa
      361 attatttcta atatagaatt atttcaaaaa ataacagaaa agattttacc taaagggaga
      421 atagacgtta cgttttatta ttttggctta tttagtaagc atcaacttga taatgctaaa
      481 gatataaaac agagatttga gactttaaag aattcaatag ctgattatga tttcgttaaa
      541 aattctaatt ataaaatata tacatcatca gatatattta attcgctatc aaaagaaaga
      601 gaatatgaat atcagtttaa aaaaatcgat aaatatagtg ccgaagtaaa tgaagaaacg
      661 gatgaaactg aagcactgat ttctttaatt ccaatacagc aattttatga ttttattaca
      721 gatgacgatg ataccataaa cacaaaatta tttgaacata atataagaga ttataaaggg
      781 aaatcaaatg tcaacaaaga tattattaat acattagata aaaaagaaag tttacaattt
      841 tggtggttaa ataatggtat aactatcatt gtagaagaaa ttaaggaatc aagttcagct
      901 aaaaaaatat ctataaaaaa tccacaaatt gtaaatgggt tacaaacctc gtattcgatt
      961 tttaattatt ttcatgagaa tagtgataaa ttaaagaaag aaaatcgctc aattttcatt
     1021 aaaataataa gaatagatcc cgaagaagag ggtgttgaac tagatataac aaaagctaca
     1081 aacaaacaaa atgaaattag agataaagat atacgtgcta atgatgaagt tcaaaaaaat
     1141 atagagttgt attttaaaac aaaaggaaaa ttttatcaaa gaaaagataa atattacaca
     1201 aatcgtagaa tgaataaaaa agatataatt actttatttg atttagcgaa atatgtatat
     1261 accatcatgt ttaaagatcc tgtttttaca agaaataatc ctggaaaact tttgaaagat
     1321 gataaatata gtaagatttt taaaattgat gatcctaaac aggattatga tatttattat
     1381 tgctgctata aaatatacga tactattaat gcatacaaca aaggaaagat aaacattgtt
     1441 tcagatgaat tttcaaaatc taattttatt catcatatag tatatatttg tgtatctata
     1501 ttacaaaata gtaaagacta tgacccaagt aatataaaaa atattaactt aaataatgta
     1561 aatgaggaat taatagacat agcatacaaa atacttaata aagttattgt taataataat
     1621 atcccaggaa gtcaagtact taaaaacatt aaagaacaac catttcttgc actcatgaga
     1681 aaagaaattg ataaatataa caaatctaag aagaataaaa aataatattt ataatgaata
     1741 atactatgag aacttattta agccctattt tttagaaaaa tcgtaattaa ataagaattt
     1801 agtgtaattc aagggtacac cctaatcata caaaataagt tattgaattt atagtaactt
     1861 aaacgtatga ttagggtgta tattaattat ggagtgaaaa tgaatgatta taggttatgc
     1921 aagagtatct tcgatagatc agaatttaga gagacagcta gataatttga aaacatttgg
     1981 cgtagagaaa atatttactg agaaacaatc gggaaagtca gtagaaaata gacacgtatt
     2041 tcaagaagca cttaactttg tgagaatggg cgatagattt gtagtagaat cgattgatcg
     2101 cttaggtcgt aattatgatg aagtgattaa cacagttaat tatttaaagg ataaagaagt
     2161 tcaattgatg attactagtt tacctatgat gaatgaagtc attggcaatc ctttattaga
     2221 taagtttatg aaagatttaa tcatacaaat attagcaatg gtttcagaac aagaacgaaa
     2281 tgaaagtaaa cgtagacaag cacaaggtat ccaagttgcg aaagaaaaag gggtatataa
     2341 agggcgccct ttactttatt cagcgaatgc taaagaccca cagaaacgta ttatttatca
     2401 tcgcgtggtg gaaatgttag aagaagggca agcaattagt aaaattgcca aagaagttaa
     2461 tattacaaga caaacagttt atagaattaa acatgataag ggattatctt gataaatgga
     2521 ttgtcaatgt aaatgagcgt tcctcacagc gtttcatttc tcattaaata ttatgaataa
     2581 tagaaaattg ataattatac aagaattgac aagaagtttc gatgtttatc ttagtttaat
     2641 gaagtgaaag ttataattgt attgaatatt atcttgtcga aaagggagat gactatggta
     2701 ggaaacgatt ttgaaagaaa aaaagatgaa ctcaatggaa ttattaaata cttctctgaa
     2761 gataatgaca atgacggttg gcatgaaagt gaaaaatcaa cagctttagg tactattgca
     2821 ctaagattag aagaaatagg ggaagaaact attgagtata atactgagga aaggaaagaa
     2881 ctacttctat tatcagcaaa agcaattagg ctcatatatt aacactcaaa tagtcattta
     2941 tatctaatta atagttataa gtataacaaa acacatacat aatatgtatg tgttttaact
     3001 gtgtctcaat aatgaacgtt acttaatttt tcaaattgat gactgaaacc aatgcgagaa
     3061 tataattgag gataccttga taatcgtttc tctgtgcccg gcataccgat taatacaaca
     3121 ccaatatcaa gttgatcata aatatctcta atagtttcca atgttttata atttaaccta
     3181 tcaacttcat caatgataat taaatcaatt tcataattcc ttggttgcaa tcttacaagt
     3241 tcttctaatc cattaaactc ctcttgcttt ttcaatgatc gatacccaat ttttcctaat
     3301 tctcttttct tatttactct tagtctttcg atttctttta tacttatact ttggttttct
     3361 aagtcactgc aatacgcaat acttaaaaat tgattattta aataaactcg aatttctgaa
     3421 atatcttgag gatcatatct tataaaaact tcttctgtta tataagcaat gagattagtg
     3481 gagaaatatc ttaacccttt aaaataaata ccatctttgt gtatttttct aggtttagtt
     3541 gtagttaata aaggagtggc tctgaccact cctttaatag ttatatttct tcaggaagat
     3601 tggcaatcca tttaatccaa cctctgattg tactacttct acgatctcta gtaacatcag
     3661 aacctacgtt atatatattg catttcttca tatagttgac agtttctttc ttactaggaa
     3721 cttgattatt cttaatataa tgtttgaatg ctagatagaa tggcttgtgt gagataattt
     3781 cagtagcaag tgatagatat ttctctttta tttctttttg cataatatct aacccttttt
     3841 tagttattct atatgcgatt tcattgtctt cgtcagtaaa cttctctatt aaacctaaat
     3901 atattcctgc atttgtgtag tatccagtct gtcttgaagt gaagtcataa ttaagagtta
     3961 tttcttcgaa cattaaatct ttttcaacta ataagttaag taaatctata actctttcga
     4021 agctgtcagc ttgtggaaac ggcgcaagtg gctctggaac ttcattagca tttttataaa
     4081 ggttacttaa gtcgtctttg atgatacttt tatgattaaa cataaaattg acttgtttca
     4141 ctaattgtag tgagttgtag ttattaatat caacaacatc ataaataaag aagctataca
     4201 catcattcga caaagtgaag aagatagggt ttattttttt attagtacga gaacttaaag
     4261 ttctaaaagg atagtataat tgacgagcca taaaatcttc agcggtatgc attttagatt
     4321 caacaattaa aaagtggttt ttactttcgt agctaccatc gatttctatt tgagaattct
     4381 ctaccgcaaa attatagtta gtattagtag ttttattaag aacatcataa gagaattttc
     4441 ctgaacccat tctaccagtg acagttaaat ctaaattttc attgtttgct agtttactaa
     4501 gcattctact gatatgtgca atatttaatg cgacactttc agatgtaacg atttgaggtt
     4561 taatggtatc gatagtatgt gggaaactaa cgatttctgg cttgatagtt tcatcatatt
     4621 taagttcttt atatacatca aagtttccta taacgtagtt cccacggcga tttggtaaaa
     4681 ttacaagatt ttcatcttta aattgtatag gtaagttatt gtaatggtca aactttgtca
     4741 ttagtcgagg ctctctaacc tttttaatat catctgcaga tatttcatag aaaccatttt
     4801 tatggcattc atcgacaata tcgtgcgttt caaatagtat ttgccatgct ttatcgtttt
     4861 ttgtatctaa tttcttctta tcagtcatat ctaacaccat cctttttagt taatttaatt
     4921 ataaatggga tggtaaaata atgaaaatat taataggagc acaaacagtt gcgtaagcat
     4981 gttattataa ataaatagaa cgagcgttcg taaataaatt taaggtgatt tatatgacta
     5041 aaaataatga aataataaaa cctatactta agtatgtagg tggtaaatca aaagttttac
     5101 cagatattaa ggcgaatatg ccatcagaat ataaccgata ttttgagcca tttttaggtg
     5161 gtgctagcgt tttattaggt attcaaccta aatcggcgat agttaatgat attaattttg
     5221 aagttattaa tctttataga gtagttaaaa acaatccata cgaattaatt gaggatttaa
     5281 aaaagcacgt gaatgaaaaa gagtattact atagcatcag agcgttagat agagatgagg
     5341 aattatttgc taaattaact gctgttgaac gtgcatcacg tatactttat attaataaaa
     5401 ctgggtttaa tggtatttta cgatattctc aaaaagggta tttaaatgta ccaatgggta
     5461 agtataaaaa ccctaatatc gtaaatgagg aaggcatttt aaaattaagt aagtacttaa
     5521 atgaaaatga tataacaatt ctaaacaccg atttcataga ggcttgtgct gatgcaaaag
     5581 aaggagactt tatatattcc gattctccat atattcccat cagtaaaaca tcagctttca
     5641 cgtcatataa tgcagatggc tttacaatag aagaccaaat tcgcttaaga gatttagcct
     5701 tagatttaga caaaagaggc tgcaaagtaa tgttgagcaa tagctcagca gatatggtgt
     5761 atgatttata tagggacttt aatattagac acgttgaagt cgcaagagca attaacagta
     5821 aaggcgatgg tcgtggtaaa attaaagagg tattaataac gaattactaa tgaaaaggag
     5881 taatttatat agcaactaca gtaaatgaaa ataaatgatt atcagaagtt tgattcacat
     5941 taaaaaggca taacaagttc atgaacttgt tatgcctttt taattgcaat tagttaccaa
     6001 ataaacgact ccaaaatctc tttttaggtg gttctgcctc cctattatca ctggaattac
     6061 tcttcgtttt ttcaacaaac ttatcctctt ttgttaattc atctgtactg tcatgcttgt
     6121 tctcttcagt tatattttta tgatttactt cagttggttg acattctttt tgttgttggt
     6181 tgatattttg agattcttca gtaaaaattg cttcttgtgg attaatattc tgtctattat
     6241 tcatcgttgt atcaaaagaa taattaagct gtctttcttc ttctaattgt ctttctagtt
     6301 tttgaatttt tttattgttt tctaaagata atatttgttg attctccaac agtttattta
     6361 atgtattaac attgctattt tgttgatcta attgtttagt tagattctca atatattttt
     6421 catcattttt agctctagtt tcaaagattt ctatttgctt ttctaattca tttacttttg
     6481 tttttaatgt ctcaaaacta tttgaattat tatgtatata cttttcaact tttgtagtat
     6541 tttcattaaa taattctttt tttactgatt ctttattatg ggttgttctc tctttcttag
     6601 tgattttttc tataattttt aaataatcat tatctttaat ataattaatt ccattttctt
     6661 tttcaaaaga tatatttaat cttttagcat tattaacaat agtttgttta gttacaccta
     6721 attcatcagc aatcatttta atagttttca taacattcac ccttaggtac ctaatttagt
     6781 acctaaattt taccacctaa caaaatttat ttcatcattt agtactaaat ttactatcta
     6841 ttaactacct aaatgcctaa ttatccaccc ataaaatgtt tttgatactt aattaggtac
     6901 taaaatatcg aattatacgt ttggtagttt taggtgtatt tttaactaca tttaaaactc
     6961 tttatatacg ccattgaaag tgttaatatt actttataaa atattaaaag agtcgatgct
     7021 acgccaatag catcgactct cggtaataaa acgattcgca ctcgtttgtt tatatatttt
     7081 tttgatactt gtattatata tatctaatca tctaagtgca agcacaaaac atataactta
     7141 cgtaaaaatt gttttattac ctcaatccca aaatggaaat gaggttttta ttatgcccaa
     7201 ttttgaaaaa tataatttat cacaagtaaa aactgaaaga ttttatcaac tgcctaaata
     7261 tttatttgaa gatgcatatt ttaagaaaat gtctgcggaa gccaaaatta tgtatgcgtt
     7321 attaaaagat cgttttgaat tatccctcca aaatgaatgg gtagataaaa ataataatat
     7381 ttactttatt ttcagtaata aacatttgtg tgaatactta ggttatgcag aacaaaaaat
     7441 tataaaattg aaaaaagagt taataaaatt taatttacta actcaagaac gtgttggtct
     7501 taataaacca aatagattat acctattaaa acctaattat gacattgaag ccagtcatat
     7561 caaggaactt ccaaattcac agttccagaa caatgaattt ggaagttcta gaactgtgaa
     7621 tttaagtggt caagaacttc caaattcaca gtctaatgat actgattata atgacactga
     7681 ttatattaag actaatagta atgatacgta tgatatgaat gataagaaaa caacatattc
     7741 tagtaatcat acaaatcatt caaatcacta ctattcaaaa ttcaatgatg aagctttaaa
     7801 atttcaatta cttgaagaac taccacaaag tattcaaact tatttaagta acttttctgt
     7861 atctgaaatt aaacttatta aatctgtgtt attaaaagcc aaaacatcct tcaacaattc
     7921 tattgatgca tattatttat tagaagatat ggaatttgaa attgttagtg ttcttaagcg
     7981 ttttaaagct acattaattc aaaaaaatga aaccgttgaa gcaatgcaag gatacttaat
     8041 gaaatctctt aaatctgaat ttgcagaagt acatacgctt aataaacgac gtgatcattt
     8101 acccatcact tctttatttg atcaataata caacttaata agctaacaat gatccaatta
     8161 gaggaatatc tttaattaga tgttctctta actgtaggcg aactttattt tcaatgaaat
     8221 gtaattaatt aagtaagtgg gactaaaatc gaaaagtttt agtctcattt ttataaacaa
     8281 ttattattca ttgttactac tatcagcaat tttttctttt tagtggaact caagtatttg
     8341 taagcatgat aaattacttc tttttaaatt tatttggctt attcgtatta atgtatttgg
     8401 catacatctt taacaattct tctgtttcat taggacttaa atgttcacat tcaaaatatt
     8461 tttcaagtat tgcgcctttt cgaataagtc tatgcgctct ttcttttcga tctaaatttt
     8521 ttaatctatg ttttcttaat tcagcttttt caattgcagc atttgttttt ttgagatttt
     8581 taaaatcatt catcttatca cttactattc tgattaattt cgttttcttt taaattttca
     8641 ttgtttctgt tactgccatt ttgttcgata tcttgattaa atacttcaat gactttatcc
     8701 atgattttat acaattcatc actagagtta atcatattta agtcaagttt atttaaaaaa
     8761 taaccaccaa ttttatgaga tagttgtgct tcttgttgtt tcttttgctc tttcagttgt
     8821 tcaatttgtt gttctaattg acttaatttc gtaatatcgg tttgtttgat tttagacata
     8881 aaaaaactcc cttaggttgt ctatctaaag cataccataa ttcattgggt aagtcgcaca
     8941 ttcatggtat aatataacca gttcttaaga cactgcccat ttacatgcaa attagaaatt
     9001 ggcataaaaa tgggcaacca tactggttga tcgttatagt tcccacaggg gtttaaattc
     9061 aaaaaagaga ggaggtaaaa tcatggagaa aggttacttt atgttaagag tgactaacat
     9121 taatcgagca actaaaaata ttgttgcgtc agcatcgtat cgttctgatg aagcattata
     9181 tagtgaacgt acagatgaaa aaattaagtt tagaaatcat atagttaaac ctgaaagcat
     9241 gattttaaca cctcaaaatg cgcctgattg gacgaaagat agacagcgtt tgtggaatga
     9301 agtcgataaa gttgaaaagc ataatgcgaa aactaaaaat cctagattag ccaaagaagt
     9361 cttattaagt ttgcctaatg attttgatag agaagtgcaa acagaattaa caaaagattt
     9421 tataaaaagt gaatttgtag acaaaggaat ggttgctgat atttcgattc atagagatga
     9481 tatgaacaac ccacacgccc atgttctact tactcaaaga ccttttaatt cagatggtac
     9541 ttgggggaaa aagacaaaaa cgagaacgca atatgatgaa aatggcgatg cgattttaaa
     9601 taaaactggt aataaagtta gaaaacaaga acgatttgcc gactttgatt tcaaagaagt
     9661 tcgtaagcat tgggaattaa aattaaatca atactcagaa cgtgaacaaa gtttaagacg
     9721 atatgatagt cgttcttttg aagcgcaagg tttagaaaaa atcgcagaaa ttccacttac
     9781 aagagaagaa tatagattag aacaaaacga aaaaaaacgc tgtgaaaaag agggtatcga
     9841 gtacaaaccc gttacttatt acggacagaa aaatgaacaa attagacgat ataatcgtgg
     9901 cgaagtatca caattattta gtgataaaga gaaagaacaa gcacaaaatg aaattagatt
     9961 atttattcaa aatgctaacg atcaagtaag cgaaaatgaa caaagttatt ctattttgtt
    10021 tgatagatat caacgtgatg ttggctattc agaagctaag gaaaccatta aaaatacgtt
    10081 tgatagtgcg tctacctttg gtcgtaaaat tcaaaatgac ttactcaaaa atgaaattaa
    10141 gagacgtcaa ttaaaattct atgctaaaaa ttacgatgaa aattatcagt ttagaaaaga
    10201 aattgaaagt aagggatata gtcaacaaga gtttgttaat attgttgaaa ctaactataa
    10261 agcgttactc aatgaaaaga ataagttagc aagtagagaa gaaaaacgta aagagattta
    10321 tcaatcagct aaagatgttt atctaactga aagtaaaaaa aataatctaa ttgttcagca
    10381 aatgtaccct aaatctcata aacaatttag tagtgatgag aaagcattta ttgttaatga
    10441 ggcacaaaaa ggtaacatta tcaaagcgtc taatgttcgg tatctattca aacaaaataa
    10501 caatattcct actaatgtat tactagaaga tacttataaa aaagcaagta aggatatttt
    10561 ctttagtgaa agacagctta aacacttaga aaaagatagc tttgaatata acgttgagtt
    10621 gcaattcatt aaggaaaaag agaaagaaat tgaaagttat aaagaacata ttgatgagga
    10681 tttaagaata gatataggcg ataaaaactt tgaaaaagta aaaggccaaa cctctaatga
    10741 gaaagttcaa ttactcatta aacttgaaaa atataacaat gtcgatcaag aagaagttat
    10801 cgaaaaacac attaatcaag tggatagaaa tgataatgac aatcgtattg aacatggaaa
    10861 tgaacaatct aatcatatta atttaccaac gataaatgcc agtgacttcc ttatgtacta
    10921 tgcgaacgaa actgaaaaac aaaacaatat taactataag aaaaagaaga agacacaaga
    10981 gagaaataaa caattgagaa gatagtaggc cgttgaaata atacaaaatt cattctttag
    11041 taataagaaa cttaaacaga tgtggtacat tgattttatt ttaaagataa cattgaaatt
    11101 cacaattata tacgggattt taacttatca aacaaattga aacgaatgtc tgcatagcgt
    11161 gagctattaa gccgaccatt cgacaagttt tgattatgtt aaaggttctg aggcttaacg
    11221 tcaataaagc aattggaata aattttatat tattatttat aatcattact ttattttgta
    11281 gtgaatttaa atttttggaa ggggaatttt atatggatcc aatagttagt aatattttga
    11341 cttctttggc tgttaattat ttttctagtt tttctgaagc aatagttaaa gaatttttca
    11401 aaaaagctat aaaagaaaaa ccagaaattg aagacgaatt aaaagctgct aagtcgtcct
    11461 atgattttga ggaaattttt caagaatcta ctggaattat tgctttaaat gcaaatgatg
    11521 acgagataaa agtatttggt ggtcttttag atgcacttag gggaattaaa tttgaccatg
    11581 gtaatgggaa agttgaaatt caaggttctg tattaaatgc acctgtatta gttacaggtg
    11641 gaactgtaaa atcgagtggt tcgacattta ttggagaaaa tacagaatta aaaagttcag
    11701 gatctagaat tttagttgga aaagattcag gtatttattt aggtggtaat tctagtattt
    11761 cacaaaatta aagaggagga gttttgtgaa atataaagtt ataaataata ttaatcgcac
    11821 agcgaaaaag tctagaaata aattattttt taaggataaa gatattaaaa atgttaggtt
    11881 tttcaattca aatttaactt attttcactt taagaaaagg gtttttaaaa acgttgattt
    11941 tttaggatgt aatttaaaac atagtgtctt tcatggcggt atatttatta attgtgtttt
    12001 ttataatact aatttatcta agtctaattt tttaggtgct aattttgtaa actgtcattt
    12061 tattaattgc aaatttatta aaactaagaa ttttaatttt aataattgta atgtaattac
    12121 tagtgatttc tcttctgtaa atgtttcaaa gaaactagaa aataaaatat ttactttaaa
    12181 tcgagttcct gcttttaaaa ggtcttatat ttttactaca aagcgttcta aagggactaa
    12241 aataaataaa gggataattg ctgttttgaa aaatgagttt agtgatcatg agttatttag
    12301 aatactttct gttgttaata ataaaacgaa tgcgcgtatt aagaatttta ttacttatgg
    12361 tcagttttat gaatttgggc gaatttattt caaaaaatga tataataatt tcgatccccc
    12421 cggcgctagt aatagcttag gtgaatgttc gacaacggat cacgttaatt tcgccagttt
    12481 ttgggaagga gtgttgctta tgatgaataa actaaaaagc aatcttgatt atgttacgga
    12541 cgtcattaca tttgtattgg tattcgttaa agttatatta ctcttagttg ggcttccttc
    12601 tcgattgctc gaggggataa cgaaaaaaga cattggaaaa ttccaatgtc ttttttcgtt
    12661 atttgaaacg agttaaaaac gagtttcttc ttgtactgat aattaatatt ttttatatta
    12721 gacagtattg tatagtgaag tgtatcttat ttaagtgttc tataaactgt tgtagtactg
    12781 acaccaactt ttttagcaat ttcaacaact gtaagttttt tttctctgta taaatcttta
    12841 atttgctttt tgatttcatt agatattgaa ggtcttcctc cattacgacc tctagctctt
    12901 gcagtcttta atcctagttt tgttcgttcg cttgctaagg tggattcatt ttcattaata
    12961 gcatataaaa tagaataaaa catatcttta gtcttttcat cactagtaga tatcttcata
    13021 tctactatat ctaacttaat atttttcgtt agtaaccatt gtgaaaaaag aacaaattct
    13081 ttaggtgttt tacctaaatg ttggagatta ttgacgacta gtgtgtcacc cttctttaaa
    13141 aattttaaac agttacttaa ttcatcaagt tctctgatat ttttatcttc actttcaatg
    13201 aatatacaat tacaaccata acttctgagt ttaagtaact gtaaatcgat tgattcactt
    13261 ttaggtagat gacgaatata accaatttta gacattaaat tatctccttt aggatgtagt
    13321 aaaatttgaa gttatctata ttttattaaa atcaagttta ttattattga tttttgatat
    13381 ttatatagac gtaaaatgac ttatcgtaag tggatatgct tgtatattcc taggtggtat
    13441 aataaatagt gaacgttttt atcgctcaga cgttaaattt aacgtctgag cgaagatata
    13501 ttattttgtt aatcggattg taacattagg taatgaaaaa ctacaaaacc gtaaattact
    13561 tgaaattaga aaggagaagt taaaaattga aaaaaaacaa aggtaaa
//
