LOCUS       CP117215               12268 bp    DNA     circular BCT 14-FEB-2023
DEFINITION  Klebsiella pneumoniae strain 2021QW-00056 plasmid p2021QW-00056_1,
            complete sequence.
ACCESSION   CP117215
VERSION     CP117215.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN21919093
KEYWORDS    .
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12268)
  AUTHORS   Johnson,J.D., Blankenship,H. and Soehnlen,M.
  TITLE     Long read genome assemblers struggle with small plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12268)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 12268)
  AUTHORS   Johnson,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2023) Bureau of Laboratories, Michigan Department
            of Health and Human Services, 3350 N Martin Luther King Blvd.,
            Lansing, MI 48906, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Trycycler v. v0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 127x
            Sequencing Technology             :: Oxford Nanopore, Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/02/2023 11:14:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,171
            CDSs (total)                      :: 5,046
            Genes (coding)                    :: 4,942
            CDSs (with protein)               :: 4,942
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 44 of 104
            Pseudo Genes (incomplete)         :: 49 of 104
            Pseudo Genes (internal stop)      :: 30 of 104
            Pseudo Genes (multiple problems)  :: 15 of 104
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12268
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2021QW-00056"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2021QW-00056_1"
                     /geo_loc_name="USA"
                     /collection_date="2021"
     gene            70..975
                     /locus_tag="M0J08_25775"
     CDS             70..975
                     /locus_tag="M0J08_25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="WDA85472.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDD
                     GRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMV
                     IQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDV
                     DRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSE
                     ARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFA
                     LDSGNEEYLAAAKAQLAAAE"
                     /name="Mph(A) family macrolide 2'-phosphotransferase"
     gene            972..2210
                     /gene="mrx(A)"
                     /locus_tag="M0J08_25780"
                     /gene_synonym="mrx"
     CDS             972..2210
                     /gene="mrx(A)"
                     /locus_tag="M0J08_25780"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="WDA85473.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWAG
                     ATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTGLI
                     ACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGAAIG
                     LLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLLRPLF
                     GIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVGARISA
                     QNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRTPPELLG
                     RVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQFATMAAA
                     ATASAPTHTEGEH"
                     /name="mrx(A)"
     gene            2210..2794
                     /gene="mphR(A)"
                     /locus_tag="M0J08_25785"
     CDS             2210..2794
                     /gene="mphR(A)"
                     /locus_tag="M0J08_25785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="WDA85474.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAALI
                     QRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVNYL
                     ISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDG
                     ELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
                     /name="mphR(A)"
     gene            complement(3287..4051)
                     /locus_tag="M0J08_25790"
     CDS             complement(3287..4051)
                     /locus_tag="M0J08_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="WDA85475.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHT
                     TIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFY
                     LSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGIR
                     GEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
                     /name="IS6-like element IS6100 family transposase"
     gene            complement(4169..4234)
                     /locus_tag="M0J08_25795"
                     /pseudo=""
     CDS             complement(4169..4234)
                     /locus_tag="M0J08_25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /name="EAL domain-containing protein"
     gene            complement(4278..4583)
                     /locus_tag="M0J08_25800"
     CDS             complement(4278..4583)
                     /locus_tag="M0J08_25800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="WDA85476.1"
                     /translation="MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTV
                     YPMLHGLEKKGYLTSRHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG"
                     /name="PadR family transcriptional regulator"
     gene            complement(4594..5799)
                     /gene="chrA"
                     /locus_tag="M0J08_25805"
     CDS             complement(4594..5799)
                     /gene="chrA"
                     /locus_tag="M0J08_25805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004896675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0015109 - chromate transmembrane transporter activity
                     [Evidence IEA]; GO_process: GO:0015703 - chromate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="WDA85477.1"
                     /translation="MTNNPTDDSRPWSVFLIFLRLGLTSFGGPIAHLGYFRAEFVTRRR
                     WLSERSYADLVALCQFLPGPASSQVGIAVGLSRAGYSGALAAWAGFTLPSAIALILFAL
                     GISSYGDYVSQGALHGLKVVAVAVVAQAVWGMARNLCTDGLRVTIMAIATCVVLLVPSA
                     WGQVGVIAIAGIAGRLLFKPAKVVEHDPLPITVSHRAGVLWLSLFFVLLIGLPVLAELM
                     PSQTMAMVDSFYRVGSLVFGGGHVVLPLLQAEVVPSGWVNNESFLAGYGAAQAVPGPLF
                     TFAAFLGASMNTAPSGWIGGIVCLLAIFAPSFLLVVGSMPFWERLRRNTGIQAALAGIN
                     AAVVGLLLAALYQPVWTSAIFQPQDFGLALVALVALMFWKLPPWLVVLGSGAAGWLLSV
                     AL"
                     /name="chrA"
     gene            complement(5955..6158)
                     /locus_tag="M0J08_25810"
     CDS             complement(5955..6158)
                     /locus_tag="M0J08_25810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDA85478.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARID
                     RTFAETFVLRSELLGIASENGK"
                     /name="hypothetical protein"
     gene            complement(6286..7125)
                     /gene="sul1"
                     /locus_tag="M0J08_25815"
     ARG             complement(6290..7125)
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(7119..7466)
                     /locus_tag="M0J08_25820"
     CDS             complement(7119..7466)
                     /locus_tag="M0J08_25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="WDA85466.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="quaternary ammonium compound efflux SMR transporter
                     QacE delta 1"
     gene            complement(7583..8421)
                     /locus_tag="M0J08_25825"
                     /pseudo=""
     CDS             complement(7583..8421)
                     /locus_tag="M0J08_25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /name="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
     gene            complement(8427..8717)
                     /locus_tag="M0J08_25830"
     CDS             complement(8427..8717)
                     /locus_tag="M0J08_25830"
                     /inference="COORDINATES: protein motif:HMM:NF017990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1010 domain-containing protein"
                     /protein_id="WDA85467.1"
                     /translation="MFIQTAFSFSGVIQCLFCLFSGLRLHGLRRFSVFLASSPCVASAS
                     SYRFCSAVPPRWRSVFSRLAPVAKFKLSVLASGSNISVKPTRILRSAYLAR"
                     /name="DUF1010 domain-containing protein"
     gene            complement(8829..9326)
                     /gene="dfrA12"
                     /locus_tag="M0J08_25835"
     ARG             complement(8833..9326)
                     /name="dfrA12"
                     /gene="dfrA12"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            9471..10484
                     /gene="intI1"
                     /locus_tag="M0J08_25840"
     CDS             9471..10484
                     /gene="intI1"
                     /locus_tag="M0J08_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="WDA85469.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
                     /name="intI1"
     gene            10687..11037
                     /locus_tag="M0J08_25845"
     CDS             10687..11037
                     /locus_tag="M0J08_25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="WDA85470.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVNQ
                     WRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATETS
                     VKPDACDSPPSG"
                     /name="DUF3330 domain-containing protein"
     gene            11163..11438
                     /locus_tag="M0J08_25850"
                     /pseudo=""
     CDS             11163..11438
                     /locus_tag="M0J08_25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="incomplete; partial on complete genome; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            11512..12216
                     /locus_tag="M0J08_25855"
     CDS             11512..12216
                     /locus_tag="M0J08_25855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology. GO_function:
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WDA85471.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             complement(7123..7466)
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             71..972
                     /name="mph(A)"
                     /gene="mph(A)"
                     /gene_name="Mph(A) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7634..8421)
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             71..975
                     /name="mphA"
                     /gene="mphA"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7631..8409)
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             973..2210
                     /name="Mrx"
                     /gene="Mrx"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 ggattgaata taaccgacgt gactgttaca tttaggtggc taaacccgtc aagccctcag
       61 gagtgaatca tgaccgtagt cacgaccgcc gatacctccc aactgtacgc acttgcagcc
      121 cgacatgggc tcaagctcca tggcccgctg actgtcaatg agcttgggct cgactatagg
      181 atcgtgatcg ccaccgtcga cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc
      241 gaggtaagcg cgaaggtcga accagaggcg cgggtgctgg caatgctcaa gaatcgcctg
      301 ccgttcgcgg tgccggactg gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc
      361 gaagactcga ctgcgatggt catccagcct ggttcgtcca cgcccgactg ggtcgtgccg
      421 caggactcgg aggtcttcgc ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc
      481 cccatttccg ccgccgtgga tgcggggatg ctcatccgta caccgacgca ggcccgtcag
      541 aaggtggccg acgacgttga ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc
      601 caccggtggc agcgctggct cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg
      661 catggcgatc tctacgtggg ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg
      721 atcgactgga gcgaggcccg cgttgatgac cctgccatcg acatggccgc gcaccttatg
      781 gtctttggtg aagaggggct cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg
      841 gtgtggccgc ggctcgccca ccacatcgcg gagcgccttg cgttcggggc ggtcacctac
      901 gcactcttcg ccctcgactc gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc
      961 gccgcagcgg aatgagcgaa cgtcgatata gcccgctcgc gacgctgttc gcggcgacct
     1021 ttctcttccg gatcggcaac gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc
     1081 atacaaagag cgcggcctgg gcgggcgcca cggccgctag cagcgtcatc gcgaccatca
     1141 tcggcgcgtg ggttggtggt ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga
     1201 tctcgggtgt ggtgggcggc gtggccatgg cgagcatccc actgctcgat gccgttggcg
     1261 ccctctcgaa cactgggctg atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg
     1321 gtatggccgc gcaggacagt gagctgccca aactcggcca cgtcgccggg ctctccgttg
     1381 agcgcgtctc gtcactgaaa gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc
     1441 ttgggggggc cgcaatcggc ctgcttggcg ctgcgccaac gctcgggctg acggcgttct
     1501 gctccgtcct tgcaggtctg ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga
     1561 tgaccacgac ggcgactctc tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc
     1621 tgctgcgccc tctctttggt atagtgatga tcttcgtggg catcgttggc gccaacggca
     1681 gcgtcatcat gcctgcgctg tttgtagatg caggacgcca agtagcagag ctcgggctgt
     1741 tctcctcaat gatgggggct ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg
     1801 cccggatatc agcgcagaac tggctggcgg tggcattttg tggctctgcg gtgggctcgc
     1861 ttctgctttc acagttgcca ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac
     1921 tgctgaccgg ctcagtctct cccattctca acgctgccat ctacaaccgc acgccgccag
     1981 aacttctcgg ccgggtactc ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg
     2041 ttatgcttgc ggccggcgcg tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat
     2101 cggccgtgtt tgcggggctc gtggctctac tctcgctccg tcttcaattt gctacaatgg
     2161 cggcggcagc cacagcctcc gccccaaccc atacagaagg tgaacactga tgccccgccc
     2221 caagctcaag tccgatgacg aggtactcga ggccgccacc gtagtgctga agcgttgcgg
     2281 tcccatagag ttcacgctca gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt
     2341 aatccagcgc ttcaccaacc gcgatacgct gctggtgagg atgatggagc gcggcgtcga
     2401 gcaggtgcgg cattacctga atgcgatacc gataggcgca gggccgcaag ggctctggga
     2461 atttttgcag gtgctcgttc ggagcatgaa cactcgcaac gacttctcgg tgaactatct
     2521 catctcctgg tacgagctcc aggtgccgga gctacgcacg cttgcgatcc agcggaaccg
     2581 cgcggtggtg gaggggatcc gcaagcgact gcccccaggt gctcctgcgg cagctgagtt
     2641 gctcctgcac tcggtcatcg ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga
     2701 gctagctgat catgtgctgg ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca
     2761 cgacgatttc caactcctcc aggcacatgc gtaaacggag gtgtgcagag tccctgcggc
     2821 aggcgacgaa cacgaccgtc gtcgattagt accggtacgg tcggtggtat cgaagtcttg
     2881 atcaccactc aggtctacgg cttacaaatg gtgaccatcc cgatacttgc gtcagagcac
     2941 cgggccgatt ctttgacagt gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg
     3001 gtcgagacga tactccgcca atgcgcccag caaacaacct ggccatcgca ggtggtgggg
     3061 agcggtgtgg cggatgagtt ggacaagttg gtgtagcagc acgagcacgg cgagataaca
     3121 tcgcaggagt tcgacatgct caagagacag ctgattgcga atcgcgatgc agattcataa
     3181 cccgattgcg ggttggcttc actccaccat caccgagcag actagcacgg cgggctctgt
     3241 tgcaaagatt ggcggcagtc agaggtaggc tgtcgctctg cgccgatcag gcggctgctg
     3301 cgaaatggtg gttgagcatg cccatggcct ccgtcagcgc cgagggccca atgccaaaag
     3361 ctctctccac aaggcgcacc tcgcccctga tgccgggctg caggcaccag gggcgagcct
     3421 gtcctttgcg cagggctcgc atgacttcga atcccttgat cgtggcatag gccgtgggga
     3481 tcgatttgaa accgcgcacc ggcttgatca gtatcttgag ctttccgtga tcggcctcga
     3541 tcacgttatt gagatacttc acctgccggt gggccgtctc ccggtccagc tttccttcgc
     3601 gcttcaattc ggtgatcgct gcaccatagc tcggcgcttt gtcggtattg agcgtggcag
     3661 gcttttccca gtgcttcagg cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg
     3721 cgctgcgggt cggcgacagg tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt
     3781 acaggtaggt ccacttgccc cgcaccttga cgtaggtttc atccaggcgc cagctcggat
     3841 caaagccacg ccgccagaac cagcgcagcc gcttctccat ctccggggcg tagcactgga
     3901 cccagcgata gatcgtcgta tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa
     3961 ggtcgcgata gctgatcgga tagcgacaat accagcgcac cgcccacagg atcacatcac
     4021 cctggaaatg gcgccacttg aaatccgtca tcgttccgtc cgtccaatct ccgccaagca
     4081 tgctcaagct tcacgatttt tgcaacagag cccacacgag tattgagcat agtcgagatt
     4141 ggtgcagatc acttctgata ttgaactgtc aggagctggc tgcacaacag ccattacgcc
     4201 caatcaactg gtgcagtcgt cttctgaaaa tgacaatcca gttagggtat agctcaacct
     4261 gacatagaag caaaaactca accaccttct accaactctc cgaacagctc cttgaccttt
     4321 gttttcgcat cagcaagtgc agttctgcct tgttcagtga tgtcataaac acgccgttca
     4381 cgtcgcccgg tgcgttcgtg gcgtgaggtc agatagcctt ttttttccag gccgtgcagc
     4441 atcgggtaca cggtgccagc gctcatctcg tagccgtgtc ggcgtagctc ttcgatgatc
     4501 cccagcccaa agacaggttc ctcggctgca tggtgaagga tgtgcaggcg gatcaaaccg
     4561 ccgtagaggt ctttgtcagt cattttttgt gcctcacaga gcgacgctca acagccaccc
     4621 agctgcaccg ctaccgagga caaccagcca cggcgggagc ttccagaaca taagtgcgac
     4681 aagggcaact aatgccaagc cgaagtcttg cggctgaaag atggcgctag tccatacagg
     4741 ctgatacagc gcggccagca gcaagccgac tacagcggca ttgatcccgg ccagcgcagc
     4801 ttggatgcct gtattgcggc gcaaacgctc ccaaaatggc attgatccga cgaccagcaa
     4861 gaacgagggc gcgaagatag ccagcagaca cacaatgccg ccgatccagc ccgacggggc
     4921 ggtgttcatc gaggcaccaa gaaacgcggc gaacgtgaac aaagggccgg gcaccgcttg
     4981 agctgccccg taccccgcga gaaaggattc attgttgacc cagccggagg gcaccacttc
     5041 ggcttgcagt aatggcagca caacgtgacc accgccgaac accagtgatc cgacacgata
     5101 gaaggaatcc accattgcca tggtttgact tggcatcagt tcggccaaca ccggcaggcc
     5161 aatcagcaag acaaagaaca gcgagagcca aagcacgccg gcccggtgac tgaccgtgat
     5221 aggtaggggg tcatgctcaa caactttcgc tggcttgaac aataaccggc ctgcgatgcc
     5281 tgcgatagca atcacgccaa cctgtcccca cgcggacggc acaagtaaaa cgacgcaggt
     5341 agcaattgcc atgatggtga ctcgcagccc atccgtgcat aggttacgcg ccatgcccca
     5401 tactgcttga gcgaccacgg ccacagccac cacttttaag ccatgcaacg cgccctgcga
     5461 gacgtaatcg ccatagctgg agatgccgag cgcaaaaagg atcaaggcta tggcagacgg
     5521 cagcgtgaag ccagcccaag cagccagcgc cccgctgtat ccagcccgag acagtcctac
     5581 cgctatgccg acctggctgc ttgcaggccc tggcaagaac tgacaaagcg cgaccaagtc
     5641 agcatagctc cgttcggaga gccagcgccg ccgtgtgaca aattcggcgc ggaagtagcc
     5701 caagtgcgca atggggccgc caaaagatgt caatccaagc cgcagaaaaa taagaaagac
     5761 cgaccatggt ctgctgtcat cggtagggtt attcgtcata ctttcgcctt catgatctgc
     5821 aacgagttga tcaataataa gcgaaattcg ataacgaaat tcgatataaa tctagaaaaa
     5881 aatacctcta tgtgtactac gcagttttag ctgtggcttt cacaggagca cgcttactta
     5941 cggcttagcg tgctttattt tccgttttct gaggcgatcc ctaggagctc ggatctcagg
     6001 acgaaggtct ccgcgaatgt ccggtcgatc cgcgcgacgt cccaggcggg cgttcccttg
     6061 gcggacatcc acgccgcagc gtcgtgcatc agccgcacaa cctcgtcgat atcacccgag
     6121 caggcgaccc gaacgttcgg aggctcctcg ctgtccattc gctcccctgg cgcggtatga
     6181 accgccgcct catagtgcag tttgatcctg acgagcccag catgtctgcg cccaccttcg
     6241 cggaacctga ccagggtccg ctagcgggcg gccggaaggt gaatgctagg catgatctaa
     6301 ccctcggtct ctggcgtcgc gactgcgaaa tttcgcgagg gtttccgaga aggtgattgc
     6361 gcttcgcaga tctccaggcg cgtgggtgcg gacgtagtca gcgccattgc cgatcgcgtg
     6421 aagttccgcc gcaaggctcg ctggacccag atcctttaca ggaaggccaa cggtggcgcc
     6481 caagaaggat ttccgcgaca ccgagaccaa tagcggaagc cccaacgccg acttcagctt
     6541 ttgaaggttc gacagcacgt gcagcgatgt ttccggtgcg gggctcaaga aaaatcccat
     6601 ccccggatcg aggatgagcc ggtcggcagc gaccccgctc cgtcgcaagg cggaaacccg
     6661 cgcctcgaag aaccgcacaa tctcgtcgag cgcgtcttcg ggtcgaaggt gaccggtgcg
     6721 ggtggcgatg ccatcccgct gcgctgagtg cataaccacc agcctgcagt ccgcctcagc
     6781 aatatcggga tagagcgcag ggtcaggaaa tccttggata tcgttcaggt agcccacgcc
     6841 gcgcttgagc gcatagcgct gggtttccgg ttggaagctg tcgattgaaa cacggtgcat
     6901 ctgatcggac agggcgtcta agagcggcgc aatacgtctg atctcatcgg ccggcgatac
     6961 aggcctcgcg tccggatggc tggcggccgg tccgacatcc acgacgtctg atccgactcg
     7021 cagcatttcg atcgccgcgg tgacagcgcc ggcggggtct agccgccggc tctcatcgaa
     7081 gaaggagtcc tcggtgagat tcagaatgcc gaacaccgtc accatggcgt cggcctccgc
     7141 agcgacttcc acgatgggga tcgggcgagc aaaaaggcag caattatgag ccccatacct
     7201 acaaagcccc acgcatcaag cttttgccca tgaagcaacc aggcaatggc tgtaattatg
     7261 acgacgccga gtcccgacca gactgcataa gcaacaccga cagggatgga tttcagaacc
     7321 agagaaagaa aataaaatgc gatgccataa ccgattatga caacggcgga aggggcaagc
     7381 ttagtaaagc cctcgctaga ttttaatgcg gatgttgcga ttacttcgcc aactattgcg
     7441 ataacaagaa aaagccagcc tttcatgata tatctcccaa tttgtgtagg gcttattatg
     7501 cacgcttaaa aataataaaa gcagacttga cctgatagtt tggctgtgag caattatgtg
     7561 cttagtgcat ctaacgccgg agttaagccg ccgcgcgtag cgcggtcggc ttgaacgaat
     7621 tgttagacat catttaccaa ctgacttgat gatctcgcct ttcacaaagc gaataaattc
     7681 ttccaagtga tctgcgcgtg aggccaagtg atcttctttt tgtcccagat aagcttgctt
     7741 agcttcaagt aagacgggct gatactgggc aggtaggcgt tttattgccc agtcggcagc
     7801 gacatccttc ggcgcgattt tgccggttat tgcgctgtac caaatgcggg acaacgtaag
     7861 cactacattt cgctcatcgc cggcccagtc gggctgcgag ttccatagct tcaaggtttc
     7921 cctcagcgcc tcgaatagat cctgttcagg aaccgggtca aagaattcct ccgctgccgg
     7981 acctaccaag gcaacgctat gttctcttgc ttttgtaagc aggatagcta gatcaatgtc
     8041 gatcatggct ggctcgaaga tacccgcaag aatgtcattg cgctgccatt ctccaaattg
     8101 cagctcgcgc ttagccggat aacgccacgg gatgatgtcg tcatgcacga caagggtgac
     8161 ttctatagcg cggagcgtct cgctctcgcc agggaaagcc gaagcctcca taagatcatt
     8221 gagcaatgct cgccgcgtcg tttcatcaag ctttacggcc acagtaacca acaaatcaat
     8281 atcgctgtat ggcttcaggc cgccatccac tgcggagccg tacaaatgca cggccagcaa
     8341 cgttgattcc agatggcgct caatgacgct tagcacctct gatagttggt tcgaaatttc
     8401 gatggtcacc gcttccctca tgatgtctaa cgggcgaggt aagccgaccg cagaatgcgg
     8461 gtcggcttga ccgaaatgtt agaaccagaa gccaaaacgg ataacttgaa tttggcgacg
     8521 ggcgctaacc gtgaaaaaac gctgcgccac cgaggcggca cagcactgca aaaacgatag
     8581 ctgcttgcgc ttgctacgca agggctagag gccaaaaaga ctgaaaacct gcgcagccca
     8641 tgcaggcgaa gcccggaaaa aaggcaaaac aggcactgaa taacgcctga aaagctaaat
     8701 gccgtttgaa taaacatgag ctaaataaag ctgggtttca gtggtgctaa cgttggacgt
     8761 aacgagagcc ggagcgcagc ggagggaacc aaaatgcgca gcattttggc gtcccgttga
     8821 cggaatggtt agccgtttcg acgcgcataa acggagtggg tgtacggaat tacagcttga
     8881 atggtttcgg ttgagacaag ctcgaattct gtttcgttga gcattgggaa gaaggcgtca
     8941 ccctcgaagg tttgatgtac ctcagataga aacacgccgt gggcgtgagg tagtgccaga
     9001 gtgtatatct cagctccgcc cgcgacgtag agttcattgc cgagttcgga tgccaaagcg
     9061 atagcgtgcg acagcgttga aacaactacg cagccagtgg cgcggtagtt agcttggcgt
     9121 gagattacca atgtgtgacg gttcggtaga ggcttgccga tagactcaaa ggtctttcgc
     9181 cccatgacaa cgacttttcc ctcagtgagt ctgcgaaaaa tcttctgctc acccggaatt
     9241 ttccagggga tattaggacc attgccaata acccgattgg ctcccatcgc agcaacgaga
     9301 taaatgcgta ctgattccga gttcatatgg ctaactttgt tttagggcga ctgccctgct
     9361 gcgtaacatc gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct
     9421 gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg
     9481 ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca
     9541 tacgctactt gcattacagc ttaccaaccg aacaggctta tgtccactgg gttcgtgcct
     9601 tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat
     9661 ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt caggcattgg
     9721 cggccttgct gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga
     9781 tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct gaccccggat gaagtggttc
     9841 gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccagcttctg tatggaacgg
     9901 gcatgcggat cagtgagggt ttgcaactgc gggtcaagga tctggatttc gatcacggca
     9961 cgatcatcgt gcgggagggc aagggctcca aggatcgggc cttgatgtta cccgagagct
    10021 tggcacccag cctgcgcgag cagctgtcgc gtgcacgggc atggtggctg aaggaccagg
    10081 ccgagggccg cagcggcgtt gcgcttcccg acgcccttga gcggaagtat ccgcgcgccg
    10141 ggcattcctg gccgtggttc tgggtttttg cgcagcacac gcattcgacc gatccacgga
    10201 gcggtgtcgt gcgtcgccat cacatgtatg accagacctt tcagcgcgcc ttcaaacgtg
    10261 ccgtagaaca agcaggcatc acgaagcccg ccacaccgca caccctccgc cactcgttcg
    10321 cgacggcctt gctccgcagc ggttacgaca ttcgaaccgt gcaggatctg ctcggccatt
    10381 ccgacgtctc tacgacgatg atttacacgc atgtgctgaa agttggcggt gccggagtgc
    10441 gctcaccgct tgatgcgctg ccgcccctca ctagtgagag gtagggcagc gcaagtcaat
    10501 cctggcggat tcactacccc tgcgcgaagg ccatcggtgc cgcatcgaac ggccggttgc
    10561 ggaaagtcct ccctgcgtcc gctgatggcc ggcagcagcc cgtcgttgcc tgatggatcc
    10621 aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa
    10681 acgacaatgg aggtggtagc cgagggtgtg gaaacacccg actgccttgc gtggttgcgg
    10741 caggcgggtt gcgacacggt gcagggtttc ctgttcgcca ggccgatgcc ggcggcggcc
    10801 ttcgtcggct tcgtcaacca atggaggaac accaccatga acgccaatga accgagcacc
    10861 agttgctgcg tgtgctgcaa ggaaatcccg ctcgatgccg ccttcacgcc ggaaggggcc
    10921 gagtacgtgg agcatttctg cgggctggag tgctatcagc gcttccaggc gcgggccagc
    10981 actgcgaccg aaaccagcgt caaaccggac gcttgtgatt cgccgccgtc aggttgaggc
    11041 ataccctaac ctgatgtcag atgccatgtg taaattgcgt caggatagga ttgaattttg
    11101 aatttattga catatctcgt tgaaggtcat agagtcttcc ctgacatttt gcagggaatt
    11161 ccatgactgg acagcgcatt gggtatatca gggtcagcac cttcgaccag aacccggaac
    11221 ggcaactgga aggcgtcaag gttgatcgcg cttttagcga caaggcatcc ggcaaggatg
    11281 tcaagcgtcc gcaactggaa gcgctgataa gcttcgcccg caccggcgac accgtggtgg
    11341 tgcatagcat ggatcgcctg gcgcgcaatc tcgatgattt gcgccggatc gtgcaaacgc
    11401 tgacacaacg cggcgtgcat atcgaattcg tcaaggaaca cctcagttgg cactgttgca
    11461 aatagtcggt ggtgataaac ttatcatccc cttttgctga tggagctgca catgaaccca
    11521 ttcaaaggcc ggcattttca gcgtgacatc attctgtggg ccgtacgctg gtactgcaaa
    11581 tacggcatca gttaccgtga gctgcaggag atgctggctg aacgcggagt gaatgtcgat
    11641 cactccacga tttaccgctg ggttcagcgt tatgcgcctg aaatggaaaa acggctgcgc
    11701 tggtactggc gtaacccttc cgatctttgc ccgtggcaca tggatgaaac ctacgtgaag
    11761 gtcaatggcc gctgggcgta tctgtaccgg gccgtcgaca gccggggccg cactgtcgat
    11821 ttttatctct cctcccgtcg taacagcaaa gctgcatacc ggtttctggg taaaatcctc
    11881 aacaacgtga agaagtggca gatcccgcga ttcatcaaca cggataaagc gcccgcctat
    11941 ggtcgcgcgc ttgctctgct caaacgcgaa ggccggtgcc cgtctgacgt tgaacaccga
    12001 cagattaagt accggaacaa cgtgattgaa tgcgatcatg gcaaactgaa acggataatc
    12061 ggcgccacgc tgggatttaa atccatgaag acggcttacg ccaccatcaa aggtattgag
    12121 gtgatgcgtg cactacgcaa aggccaggcc tcagcatttt attatggtga tcccctgggc
    12181 gaaatgcgcc tggtaagcag agtttttgaa atgtaaggcc tttgaataag acaaaaggct
    12241 gcctcatcgc taactttgca acagtgcc
//
