LOCUS       CP117210                4715 bp    DNA     circular BCT 14-FEB-2023
DEFINITION  Escherichia coli strain 2021QW-00057 plasmid p2021QW-00057_4,
            complete sequence.
ACCESSION   CP117210
VERSION     CP117210.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN22066472
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Johnson,J.D., Blankenship,H. and Soehnlen,M.
  TITLE     Long read genome assemblers struggle with small plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 4715)
  AUTHORS   Johnson,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2023) Bureau of Laboratories, Michigan Department
            of Health and Human Services, 3350 N Martin Luther King Blvd.,
            Lansing, MI 48906, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Trycycler v. v0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140x
            Sequencing Technology             :: Oxford Nanopore, Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/06/2023 11:07:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,145
            CDSs (total)                      :: 5,027
            Genes (coding)                    :: 4,828
            CDSs (with protein)               :: 4,828
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 69 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 43 of 199
            Pseudo Genes (multiple problems)  :: 43 of 199
            Pseudo Genes (short protein)      :: 5 of 199
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2021QW-00057"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2021QW-00057_4"
                     /geo_loc_name="USA"
                     /collection_date="2021"
     gene            complement(764..1417)
                     /locus_tag="M0L40_00360"
     CDS             complement(764..1417)
                     /locus_tag="M0L40_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WDA85547.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="PP2C family serine/threonine-protein phosphatase"
     gene            complement(1414..2280)
                     /locus_tag="M0L40_00365"
     CDS             complement(1414..2280)
                     /locus_tag="M0L40_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WDA85548.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            complement(2264..3220)
                     /locus_tag="M0L40_00370"
     CDS             complement(2264..3220)
                     /locus_tag="M0L40_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDA85549.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWKQEAGKY
                     RGSSKVLSPKSPFSPYFDEEWFISINVDMVKSYGKRN"
                     /name="hypothetical protein"
     gene            4296..4490
                     /locus_tag="M0L40_00375"
     CDS             4296..4490
                     /locus_tag="M0L40_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WDA85546.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3782..4037)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.899"
                     /coverage="89.0"
                     /evalue="2.61e-118"
     Typing          complement(4162..4260)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(4564..4648)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          2..501
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.02"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 ggcgcttttc cgcttcctcg ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag
       61 cggtactgac ttacacaaaa acggcagcac ggttatccac agaatcaggg gataaggccg
      121 gaaagaacat gtgagcaaaa gaccaagaac aggaagaagg ccgcgccgca ggcgtttttc
      181 cataggctcc gcccccctga cgagcatcac agaaatcgac gctcaagtca gaggtggcga
      241 aacccgacag gacttaaaga taccagacgt ttccccctgg tggctccctc gtgcgctctc
      301 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      361 cgctttctca tagctcacgc tgttggtatc tcagttcggt gtaggtcgtt cgctccaagc
      421 tgggctgtgt gcacgaaccc cccgttcagc ccgactgctg cgccttatcc ggtaactatc
      481 gtcttgagtc caacccggta agacacgcct taacgccact ggcagcagcc actggtaact
      541 gatttagagg agttagtctt gaagtcatgc gccagttaag gctaaactga aagaacaggt
      601 tttggtgact gcgctcctcc aagccagtta ccttggttct tcgagccgca aactctttga
      661 acctaagaga aaaccgcccc gcaaaggcgg tttttttctt ttacagaaaa gagattatct
      721 atatgacacg aagatctcaa tagcttcttc aaaaaacacg aaatcaagaa gggaatctaa
      781 cgtagcaaac cgtatgattc tctgcgttac ccgggatatt tttagtaaat ttagccaagt
      841 tagattctgg gttatctttc atgaggtcaa taacagtacc atcattaaaa tgctcccaaa
      901 acccatcagt acataaaata acctctttgt tttcatcatt taaatcaaaa actgaatgat
      961 tgaaaattat ctcatcagag cagaaatcaa caaatgatgt tagataattt ctgtctgggc
     1021 aataacaaac gaggtgaggt atttttttat tatcgattcc attttccttt aatcttttcc
     1081 aggcaaccga gttatcctct gttagaagga ctccactttt gttatataca cggcaatctc
     1141 ctgcgttata tatatggagg tgcgtattat ttttaaaaag gaaacatact gcagccttaa
     1201 caggaactga cttgcatggt tcaataatat ctttattcag atcaccagaa aacacgcact
     1261 tatctaacct accagagaga attttcatat tccactcaga gcaggaatac ccatccataa
     1321 taaccattcc aaacacatca ttttgacaat ttatcgtaga taataagtct aagttttcac
     1381 atctgttacc aatatcacta aaactgaacg ctatcatttg aatatatccc acggaagact
     1441 ttcatagaaa caggcaactt tttcataagc atcttcaaaa agaggtaatg atttaacggt
     1501 ggatgataaa tcctgatatc gctgactgga acgctcgata atttctacat cccttatacc
     1561 tttaacgttt aagagattat ccaattgttt accttcactc ttttttaaaa gagactccag
     1621 tattttatac ttatcttgcc gagaaaactc atatttacct aaaagataat tgatatcata
     1681 tatatcttgc tctcgatttc tctctctaac tttttgttgc aaaacggatc gatatttttc
     1741 agccatcaaa tcagttaagc tgtaggtctt tacgctatca ttgtcatcat caccatctaa
     1801 tgataaaaag tcaaaatcat acgttttttc gttaaagcta aaatcgatat ttatcactga
     1861 aggtgattga ttagattcaa gccttttcat tgcagacgca ttagaacgat cgcaaaaggc
     1921 tatatttatc tgtacagttg gaaatgttcc ttgtgcatta ggttgaattt tatgagattg
     1981 aactttacat ttaataccat atcccaattc agcctcagca acatccattg agtcattaaa
     2041 cctattgaaa aaatccccca agtcaatttc agaaagtttt ttttcggtag aaaaatcaat
     2101 atcctttgtg tatctatcac taccatgctt aacaccaaga agagtccctc ccttcataac
     2161 catcatttca ttcaaactct cctctataga aatagattta agtattatat gtacagcctg
     2221 gcgcatcaac ctgtacttcc caccagactc ttcgatccaa ttattaattt ctttttccat
     2281 aagacttaac catatcaaca ttaatagaaa taaaccattc ttcatcaaag taaggtgaga
     2341 atggactctt tggagatagc actttcgaac tacctctgta tttacccgcc tcttgtttcc
     2401 attcgcttat ttttttatta ttaaccccaa taattctatc caaaatgaat ccaaccctta
     2461 ccttgtcaat atgacggcca agcttatcga cttcctttat aatcagaggt gcatattttt
     2521 taccgtgctc aataaatgat tccaatacat gctcctcacc accacagtaa tcaggccgcc
     2581 tggtcatatc cataaaggtc ttaccgatag atgataccct gacaggggaa ttacgagcct
     2641 tcattggctc aacataattt gtttcttgtg taacgataat ttttattcca ttgacctcag
     2701 aatttcttgg aaatctaggc atgaattgtt tggcttcgtc tatatcagga ttatcctttt
     2761 gaaaatcatg atagaactta ttcttccact gttcggaacg acatgtcgta aagtggatta
     2821 tcttgggtat tttatctgtg atgttatgcc aatccatagc ggataaatag gataagtatc
     2881 catatggata cgtagagcac actatatcat tttccggaga accatcgtta cctttaacaa
     2941 aaaagacctc atagaaaccg ccaattaaat gggggtaagc atgatgctta tcgttacttt
     3001 caactcttga taaaacccct gactttaata attttaatat atttttttca aattcattag
     3061 gtcctgcttt gttctttcta atttgatata tcctctcacc attatattct ttttttaaat
     3121 aaaaaaggta aatctgcaaa gagatttctt ctttggttat gactggtttt ttatattcat
     3181 tcaacaacca agacgttagc gcttcttcga taagatacat attcaacctt ttcggtttag
     3241 tctctaatag aatatacgtc ctaaggacgt atattctatt agagattgat tgtcaagaaa
     3301 aaaataataa caaacaagca tttgccattt ttatattttt gatatcctat aaaataaatc
     3361 ctaaggattt attttatagg ataataagta ggaagtcaag aaaaagttta cttatatcac
     3421 cgttttcata acacctcatg cgatcaattc agcggcggtt atagttcaaa tccatgatta
     3481 ggacatctgt tgctcataaa cgatgagcgt aaaagatcat ttttcagcgt catttaccat
     3541 aacgtcgcat tgcgcgcacc gctaaaacgc gctgaaagcg attctgccgc gaacaccgca
     3601 aaaagagatg ttctccccgg ggtaaaccgg aaaaatgcgt caggatcgct ttcagctcac
     3661 tgcatagcta tgcatgaaag tgaatgacga tcggtttggg cgcatacagt gcccatgaga
     3721 cacattttcg gacattttca gttcgtgaat ccctgactca ttgctacata tctgaacaga
     3781 cacccctagc agcgccccca ggggtgtcct gtaaaaaaac acaccgcgcc gctagcagca
     3841 cccctaatat aagatgatgt tttttatgaa aatagtcagt accaccccta aaaagcggtg
     3901 ttggcgcagc tacaacaccg cgccgacacc gcttttttaa atatcacaaa gagagcaaga
     3961 agaactaatt tgtcataact ctctatttat aaagaaaaat cagcaaaaac ttgtttttgc
     4021 gtggggtgtg ggcatttcag gcatgagaac agactcgcgt ttttccggag tatcagcgga
     4081 attattgatt aagaaacact cgacgcagag cgaaatttta tgcaacagcg gctgcactgc
     4141 acagggatta tttatcagtt gatatgctgt gccatgatac gtcctaaaaa aacaggacag
     4201 gatgtgcaat tgtaataccg tcgcccgcaa cgtcttacaa ttaccacctg gtcagggtcc
     4261 acccgacacc cggtactgac ggagaacaac ggaaggtgaa caaacagcaa caaactgcgc
     4321 tgaatatggc gcgatttatc aaaagccaga gcctgacact gcttgaaaaa ctggatgctc
     4381 tggatgccga cgaacaggcg gccatgtgcg aacgactgca cgaactcgcg gaagaactcc
     4441 agaacagcat ccaggttcgc tttgagaccg aaagtgaaac aggaacataa cgaagctgac
     4501 ggaggcgctc acggcttgtc cgcgagcgtt ttcggcagca gacaagcccg ccagggcgcg
     4561 tcagcgggtg tcggggcgaa gccctgaccc agtcacgtag cgatagcgga gtgtatactg
     4621 gcttaactat gcggcatcag tgcgaattgt atgaaaagtg ccccatgccg ggtgtgaaat
     4681 tccgcacaga tgcgtaagga gaaaatgcgc gtcca
//
