LOCUS       CP117209                2101 bp    DNA     circular BCT 14-FEB-2023
DEFINITION  Escherichia coli strain 2021QW-00057 plasmid p2021QW-00057_1,
            complete sequence.
ACCESSION   CP117209
VERSION     CP117209.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN22066472
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Johnson,J.D., Blankenship,H. and Soehnlen,M.
  TITLE     Long read genome assemblers struggle with small plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 2101)
  AUTHORS   Johnson,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2023) Bureau of Laboratories, Michigan Department
            of Health and Human Services, 3350 N Martin Luther King Blvd.,
            Lansing, MI 48906, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Trycycler v. v0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140x
            Sequencing Technology             :: Oxford Nanopore, Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/06/2023 11:07:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,145
            CDSs (total)                      :: 5,027
            Genes (coding)                    :: 4,828
            CDSs (with protein)               :: 4,828
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 69 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 43 of 199
            Pseudo Genes (multiple problems)  :: 43 of 199
            Pseudo Genes (short protein)      :: 5 of 199
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2021QW-00057"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2021QW-00057_1"
                     /geo_loc_name="USA"
                     /collection_date="2021"
     gene            complement(66..332)
                     /locus_tag="M0L40_00350"
     CDS             complement(66..332)
                     /locus_tag="M0L40_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WDA85544.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(336..1301)
                     /locus_tag="M0L40_00355"
     CDS             complement(336..1301)
                     /locus_tag="M0L40_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WDA85545.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     QDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(677..908)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 agcactagag attgcatgca atctcgtacc tatcacctca gcctgccgct ggctcttttc
       61 cagaactaag aatgcgttct taaaaatcca aacttgatac cgtttaaatc tattttcaca
      121 aaaagagttt ttaccctctc gtttgttttc tgaatatata aaattaattc atcctcaaaa
      181 ccttcgtgcg gatgaaaacc aaatgtgcca aaacttaatc catcaataag caaaataagg
      241 tttttaaacc tgtcaggaat caccgcttca cttcttaaaa aaaataaacc aatagtctta
      301 tttccattca ggtattcaat attaatattc atacgttaat ccgatttatc cttctcaggg
      361 gcgcgtttgt aacgccgttt acctgaatcc cagacaaacg ccgtccgctt cccgtcatca
      421 gtgccatccc ctacatcgtc ggcaatgaca agatcctgat tggtttctcg atccaactgg
      481 aggacgtttt ttagcgcccc accggtcgag ataaaacggc gcttgtgaag ctgccgcgtc
      541 agctcaagaa accactcagg atcgtttgcc atatcttccg gtttaacgga gtatttcagc
      601 gtttcagcaa ccgcgctttg cagttgctcg gcaacgttgg ccacaacctc acctgtctta
      661 gtttttactg cccgaatatc gatattcggc tcatagttca cccgcaagca atcgcgccag
      721 agttctaccc aacgttcgtg cttaacgtag ttcttccctt taaaccaaga aggttgcacc
      781 atcagcagac agtgaaaatg cggatgtgcg ctgccatcct tacctcgcgt cacctccgta
      841 gccctgatcc acccctgaac aggtgatagc tcctttcgct tttccattcg cttaaacgcc
      901 gcattcattg ctgtaaggac tgttccaagt tcacctatct cgcagttcct gacagttaac
      961 gtcagaaaca accatcggga agacgggtaa tccacaacga ttttcggtag agcctgataa
     1021 aaacgggctt gccacatgag ggttcttctc cactggcaga cagggcaatg acggacacgg
     1081 caaaattgcg cactacgaag ccgtaggcgc gtttctccgg tttccatgat ggtagaccaa
     1141 ccaaaacgta acaaaccgct acaggaggcc atacggagtg catagcgctc gaattcagca
     1201 gcagtctggt agataccccc gacagaatcg gtaagcgtgc gctgggtatc ccaaactgca
     1261 tcacgggggc tgtagtccga aaggaattta tcttctgaca ttaagcctgc tccgatctgg
     1321 gcaggctgtg atttgatgtg gtatattcat cacgtacctg tgcattgcga gccagtacat
     1381 gtcaccagag tttgccgctg aggtgacaca ttcgaaatgt tggggagtgt tgacgcactc
     1441 cccaacgccg attaaaaacg cctcaattct gctccatttt ctttgacctg tcaaaaccca
     1501 ctcaaagcct cgtcattact gggctaaacc actatttcat atccgccatt tatcccttaa
     1561 gtataaatac aaggatcacg cgcgagaatt gcgcaaaaaa taaccacaat tttaaccccc
     1621 caaaacaagc acccccgaac ggcttacccc aaccttttcc acacacccga tcaccaaaac
     1681 agggaataaa aacaaaaccc tgtcttacag gtggatggcc tcacttcgtg aaaccatcca
     1741 ccccgcataa gttttttaat cggtggtgtc gtcaatcact aaatcggaac cccaaagggg
     1801 acccccacga tttagcattt gacgagatta actctcggag atggcatcat gcttttaacg
     1861 gactttaaaa aacggtgaac ggatgcggat tttaatccgt gtctgtgagg cgttgagcaa
     1921 cgagcaaagc gaggcggtag aaagagagcc agcggcaggc tcaggacgat aatccgggga
     1981 aaatgtacca ctcgcagagt gttaacattt gacatcggat tgtctatcct gcattggtgt
     2041 cgagttggca aggctgagga taatgctgag gataatgctg aggataatgc aggatagaca
     2101 g
//
