LOCUS       CP117207                4084 bp    DNA     circular BCT 14-FEB-2023
DEFINITION  Escherichia coli strain 2021QW-00057 plasmid p2021QW-00057_3,
            complete sequence.
ACCESSION   CP117207
VERSION     CP117207.1
DBLINK      BioProject: PRJNA288601
            BioSample: SAMN22066472
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Johnson,J.D., Blankenship,H. and Soehnlen,M.
  TITLE     Long read genome assemblers struggle with small plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4084)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2022) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
REFERENCE   3  (bases 1 to 4084)
  AUTHORS   Johnson,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2023) Bureau of Laboratories, Michigan Department
            of Health and Human Services, 3350 N Martin Luther King Blvd.,
            Lansing, MI 48906, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Trycycler v. v0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140x
            Sequencing Technology             :: Oxford Nanopore, Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/06/2023 11:07:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,145
            CDSs (total)                      :: 5,027
            Genes (coding)                    :: 4,828
            CDSs (with protein)               :: 4,828
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 199
            CDSs (without protein)            :: 199
            Pseudo Genes (ambiguous residues) :: 0 of 199
            Pseudo Genes (frameshifted)       :: 69 of 199
            Pseudo Genes (incomplete)         :: 131 of 199
            Pseudo Genes (internal stop)      :: 43 of 199
            Pseudo Genes (multiple problems)  :: 43 of 199
            Pseudo Genes (short protein)      :: 5 of 199
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2021QW-00057"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p2021QW-00057_3"
                     /geo_loc_name="USA"
                     /collection_date="2021"
     gene            1..702
                     /locus_tag="M0L40_00320"
     CDS             1..702
                     /locus_tag="M0L40_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WDA85539.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLAVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(960..3119)
                     /locus_tag="M0L40_00325"
     CDS             complement(960..3119)
                     /locus_tag="M0L40_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WDA85540.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSLMPDERTGLIHDYTRK
                     SGVAEAVILTPATAPTWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALTNAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYSSEQDKIQSLSQRMEKLRLHYRKVEKQDIPASQMVFETQWQQWGASGLAALRAQL
                     LKREEELRRREQEDTERRRAERLAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRSRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSA
                     TFEQLLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAIPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRENRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="MobA/MobL family protein"
     gene            3298..3552
                     /gene="mobC"
                     /locus_tag="M0L40_00330"
     CDS             3298..3552
                     /gene="mobC"
                     /locus_tag="M0L40_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WDA85538.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2762..3120)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.444"
                     /coverage="100.0"
                     /evalue="1.88e-155"
     Typing          3121..3297
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2..702
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(964..3206)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.524"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtcggatc taattgccta taaatccaat gcgctggttg aagccagtta taaattgacc
       61 ctacaggaac agcgattttt gttactttgc atcggtcgat tgaagtctgg ggctgatgca
      121 gaatctccaa aattgcaaaa gacaatgacc attacagctg cggaatattt cgattcattc
      181 cctgacatgg ggcggaaaaa tgccgaggtt cagttacagg aagcaattga tcgtttgtgg
      241 gacaggtcaa tcattctcaa ggatgacgag aaacgtgagg agttccgctg gatccagtat
      301 cgggcacagt acgctaaagg cgaggccaga gcacaaataa cattttctga cgcagtaatg
      361 ccttatttga cacaactaca agggcaattt actagagttg taatcaagaa tatatctaga
      421 ttatcaagaa gttacagtat aaggatatac gaactcctcc agcagttccg cagtactggc
      481 gaacgtatta tcgctctcga tgattttcgc tcaatgcttg atatagagca taaatatcaa
      541 acatataaat cacttaacca gcaattatta agaccctgca ttgacgaact gaacaaaaaa
      601 agcgacctgg ctgtaacggt tgagacgatc aaaaaggggc gcacggttgt agccctgcat
      661 tttcgcttca aggaagacaa acaaatcaag atgacgatct aaaatcgatt ttaaggcacc
      721 taaaacgctc cctgctgcgt tttttttgtg ttagtagtgc gaaggtacta ttgaagtgaa
      781 aaattgcgtc aaaagcgctc taatgcagcg aaaaaagaac ccccattttt tgggggcgga
      841 aaaacaaaac tatgcttgca ttatgtgtgt gtgcttcttg cattcatccc tgtcgatgaa
      901 catcgaagaa catcgacgaa cataacacga tgttaaataa aacaaaatat tgttcacatt
      961 cactgtgacc aaccgcgatc atgtctcctc tcggtggcgt gtgttcgtgc atcttcctcg
     1021 cgccgccatt cgtcccaggt acgattaaaa cccagcgagc ggaacagatg atccggctgt
     1081 ttttctggtg tgttgacggc agcaaaaagc ggtgcctcac gggttttcag atgaaactcc
     1141 ataagttccg gcgcaggtga tatgtggctg tctgcatcgt gatttacggc aacaccatac
     1201 cctgcatacc gtaacccctt atcggtcagt gcactgatga ccagctggct gttatcggct
     1261 gagggaacgg caaacgcggg atacagcgta ccattaagcc ggacaacacc gcccagagag
     1321 tcatccacgc ccatgaccag caaaagctga agctggcggt tttcccggtc gacagaactc
     1381 attaccggca tcagcgtcgg tggcagacgt ccctgcgtaa aatccacctt ccttgccgcc
     1441 agtgatggaa tggctggctg gcggtcttct ccaagacaga aagactccgg ccatgttttc
     1501 ggctggcgga gattacgggt atgttgcggc catgtctggc aaacctgcgt cagcagttgc
     1561 tcaaaggtcg cagaggaata aacacgttgc agtggtggac gttgaccgat ttcgcttcgg
     1621 tcacagatat aaccactcct gtcacgcatt aaatcaacac tctcaaaaat tctggagcgg
     1681 tatttctcca gctcgttccc ccagagcgta tattctccgt tccggttgtg aagcagaagg
     1741 taacaggtca tttccccgtt acctggcatg ggagcctcgc cataccccgt aacgactgct
     1801 ctcagtaccc ctgaatccat aacggcattg tcatttttcc tgagctgctc agccagtcgc
     1861 tccgcgcgac gacgttctgt atcctcctgc tccctccggc gcaattcctc ttcacgttta
     1921 agcaactgag ctctgagcgc tgcaaggccg gaagcccccc actgctgcca ctgtgtttca
     1981 aataccatct gactcgcggg aatgtcctgt ttctcaacct tccggtaatg cagccgtaat
     2041 ttttccatgc gctggctcag cgactgaatt ttgtcctgtt cgctgctgta ttccggtgca
     2101 gcccattgca tcaggggaat acgggaaaaa ccggacgaaa acggcgatga ttttttcagg
     2161 ctggcagcat ggctcatcgc ttcctttcgt tcggtgttca gctcttccca gcgtctgcgc
     2221 gtcgcctgca tatctgccat catcgacatg gcattactgg tgagtttcca tgccgcgtta
     2281 ctgtccttcg gcaacgcttc gatgatgtca cgtagtgtgt acgaagttgc gttgtcagga
     2341 agcacgatat tcagaaggtc agccacacgg cgggcaggct ctgtcagccc gctgttcctg
     2401 agttcggtac gggaaatctg tatttccagg ttaagcctgt taatcagacg attctgttcg
     2461 ccgcgttctg tctcgatacc gcgtgtttcc atcgcacagg cagactttcc aaggtgaata
     2521 ccaggcgttt tctcaagccc ctgtcgctcg tatgaacgat ggtctatctc ttcctgataa
     2581 ccggcgttcg tcagtgccct gttcgcatgg tcagcccatg atgcccgcca tgtctcggca
     2641 tgtgtccggt cgttccagtc cctgaccttt cctccaaatc ccgcaggtcc gacagctctt
     2701 gtggtcagca tgatgtgcgc atggggattt gttttatcca tgtgatggaa cgccacatca
     2761 gcaatcatgc cctgactgac aaagttttcc ctgacaaagg cgagaacggt ttcccgtgcg
     2821 gcatcctgcg gcagttcgcg ggggatggcg agttcaattt cccgcgcaag ttgtgagttc
     2881 ttccggcgct cagccttctc aacagcattc cagagttcgg cacggttcgt acaccaggtg
     2941 ggggcggttg cgggagtcag aatcacggcc tcagccacgc cgcttttgcg ggtgtaatcg
     3001 tgaatcagtc cggtacgctc atcaggcatc agtgaaccgg aacgataggc agcagaagca
     3061 acggcgctgt agccgttttt tcgcgaaatg attttcatgc tgagatgata aatcgccatt
     3121 tctgccgtaa tgcctttttg tggttttctt tcagccccgc agggcgcaca caatcgaaac
     3181 ggagtggaga ttgtataagt gcgcacttcg tgattttccg tgcattatgc cagctcagtt
     3241 tttaaactac cagtgacgcg ctaaaatgcg atttttcatc gcgcacggag ttacatcatg
     3301 agcagaacac tcgaacagaa gattgctgat gctgaggcca gattacagcg cctgaaggcg
     3361 aagagcagga gtctggacac agcgcagaag gtcattgtgg gtgcagcatt gctggcaaag
     3421 gtcagaaagc cggaggaggt gcagttgcgt gcctggttac tccagttcct gaaggcagag
     3481 gtgacacgac aggctgatgt gacgcgcata ctgccgctga ttaacgagct ggaagcgctg
     3541 ccaggacagt gattgtatcg tccgctcgcc gcagtcccag cgaccgagcg ttagcgagcg
     3601 agtgggcgag gaagcggaag tgaatccgtc gcctgattga gcacatacgc acggtttttc
     3661 gggaggtgat atgcgctgaa cccgcatgaa tactggattt ccacagggtt ggagggggtg
     3721 tattcggaac ttacaattct gcttttttgc aacgcattgt ttttattggt tttttgtttt
     3781 cagcactcca agttatccac aggctcgtcg ttaagttttt agatcttgtt tttaagatct
     3841 tgtttttaat acttgtttta gcgccaaaat tccttataga aaacaaaatg ttacaccgct
     3901 ataggtaata tgatttaagg taaaggtaat atgatttaag gtgtttaggt aatatgattt
     3961 aaggtaaagg caatatgatt taaggtaata ttcacagaaa aagggatatt aaaataaaaa
     4021 ggcattgcct ttcgttgagg gtatgtttat cttttattca ttaccttttg gggagggatg
     4081 ctct
//
