LOCUS       CP115357                5462 bp    DNA     circular BCT 30-AUG-2023
DEFINITION  Escherichia coli strain CUVET16-707 plasmid pCUVET16-707.1, complete
            sequence.
ACCESSION   CP115357
VERSION     CP115357.1
DBLINK      BioProject: PRJNA914526
            BioSample: SAMN32339845
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2023 14:56:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,842
            CDSs (total)                      :: 4,728
            Genes (coding)                    :: 4,215
            CDSs (with protein)               :: 4,215
            Genes (RNA)                       :: 114
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 513
            CDSs (without protein)            :: 513
            Pseudo Genes (ambiguous residues) :: 0 of 513
            Pseudo Genes (frameshifted)       :: 407 of 513
            Pseudo Genes (incomplete)         :: 125 of 513
            Pseudo Genes (internal stop)      :: 42 of 513
            Pseudo Genes (multiple problems)  :: 53 of 513
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET16-707"
                     /isolation_source="Prostatic abscess"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET16-707.1"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2016-10-28"
                     /collected_by="Pattrarat Chanchaithong"
     gene            3..98
                     /locus_tag="PCS85_24185"
                     /pseudo=""
     CDS             3..98
                     /locus_tag="PCS85_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="incomplete; partial on complete genome; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            193..837
                     /locus_tag="PCS85_24190"
     CDS             193..837
                     /locus_tag="PCS85_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB6"
                     /protein_id="WMO74189.1"
                     /translation="MALALVGEKIDRNRFTGEKIENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNASALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGAQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDNYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB6"
     gene            complement(929..1834)
                     /locus_tag="PCS85_24195"
     CDS             complement(929..1834)
                     /locus_tag="PCS85_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="WMO74190.1"
                     /translation="MAGLNKEKKTINKTNSARFPAPPFQQQQQPFPGLAGKMQPRPDHG
                     EDSYQGSGRLNGRKVLITGGDSGIGRAVAIAYAREGADVAINYLPEEEDDAREVVDLIK
                     KAGRNVLVPGDIRDEAFCGHLVTQAVKGLGGLDILVNNAGRQQFCESIEELTTEDFDAT
                     FKTNVYAMFWITKAAIPHLSPDSVIINTSSVQAYEPSEILLDYAQTKAAIVAFTKSLAK
                     QLAPKGIRVNAVAPGPYWTVLQCCGGQPQEKVEKFGANAPLGRPGQPVEIAPLYVTLAA
                     RENSYTSGQVWCSDGGTGTL"
                     /name="SDR family oxidoreductase"
     gene            complement(2299..3839)
                     /locus_tag="PCS85_24200"
                     /pseudo=""
     CDS             complement(2299..3839)
                     /locus_tag="PCS85_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050481.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISCR1 family transposase"
                     /name="IS91-like element ISCR1 family transposase"
     gene            complement(4244..5083)
                     /gene="sul1"
                     /locus_tag="PCS85_24205"
     ARG             complement(4248..5083)
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(5077..5424)
                     /locus_tag="PCS85_24210"
     CDS             complement(5077..5424)
                     /locus_tag="PCS85_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="WMO74188.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="quaternary ammonium compound efflux SMR transporter
                     QacE delta 1"
     ARG             complement(5081..5424)
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             194..834
                     /name="qnrB6"
                     /gene="qnrB6"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB6"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             158..837
                     /name="QnrB6"
                     /gene="QnrB6"
                     /gene_name="quinolone resistance protein (qnr)"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 gcttacagca ggcaaaattt aaccagaaaa aagccgctga attactgggg ctgacttacc
       61 atcagcttcg cgctttgctg aaaaaacacc agatttaacg cacatttgca gatgtcatat
      121 tggcggattt gacgcataac cccatcaggg tttaccatga cgccattact gtataaaaaa
      181 acaggtacaa atatggctct ggcactcgtt ggcgaaaaaa ttgacagaaa ccgtttcacc
      241 ggtgagaaaa ttgaaaatag tacatttttt aactgtgatt tttcaggtgc cgacctgagc
      301 ggcactgaat ttatcggctg tcagttctat gatcgtgaaa gccagaaagg gtgcaatttt
      361 agtcgtgcga tgctgaaaga tgccattttt aaaagctgtg atttatccat ggcggatttt
      421 cgcaatgcca gtgcgctggg cattgaaatt cgccactgcc gcgcacaagg cgcagatttc
      481 cgcggcgcaa gctttatgaa tatgatcacc acgcgcacct ggttttgtag cgcatatatc
      541 acgaatacca atctaagcta cgccaatttt tcgaaagtcg tgttggaaaa gtgtgagctg
      601 tgggaaaacc gttggatggg tgcccaggta ctgggcgcga cgttcagtgg ttcagatctc
      661 tccggcggcg agttttcgac tttcgactgg cgagcagcga acttcacaca ttgcgatctg
      721 accaattcgg agttgggtga cttagatatt cggggcgttg atttacaagg cgttaagctg
      781 gacaactacc aggcgtcgtt gctcatggag cggcttggca tcgcggtgat tggttagcat
      841 tcacggagcg gtaagtaccg ctcccgccct tatacaatag tgtccgccct tgccttaaaa
      901 gtcactttcg acgcacagag cgtaaacgtt agagggttcc ggtcccccca tcagaacacc
      961 agacctgacc agacgtatag ctgttctccc gagcggccag ggtgacataa agcggcgcga
     1021 tttccaccgg ttgaccaggg cgtcccagcg gcgcatttgc cccaaatttc tccacctttt
     1081 cctgcggttg accaccacag cactgcagta cagtccagta tggacccggc gcgacggcat
     1141 tgacacggat ccctttcggg gccagctgtt tcgccagcga tttagtaaat gccacgatag
     1201 ctgctttagt ctgagcataa tcaagcaaga tttcacttgg ctcataagcc tgtacggagg
     1261 aggtattaat tatcacgctg tctggtgaaa gatggggtat ggccgctttg gtgatccaaa
     1321 acatagcgta aacattggtt ttgaatgttg cgtcgaagtc ttctgtggtg agttcctcaa
     1381 ttgactcaca aaattgctga cgacccgcat tattgacgag gatatccaac cctcccagtc
     1441 cttttaccgc ctgtgttacc agatgaccgc aaaaagcctc atcacggata tcgccgggga
     1501 ccaaaacatt tctgcctgct ttttttatca gatcgaccac ttcacgcgca tcgtcttctt
     1561 cttcaggtaa atagttaatc gcaacatcag cgccttcacg agcataggca atagcaacag
     1621 cacgaccaat ccctgaatcc ccaccggtaa ttaatacctt gcgcccattc aggcgaccgc
     1681 ttccctgata gctgtcttct ccatgatcgg ggcgcggttg cattttcccc gccagcccgg
     1741 gaaagggctg ctgctgctgt tgaaaaggcg gcgccggaaa acgcgcagaa ttggttttat
     1801 tgatggtctt tttttccttg tttaagccag ccattcaatt acctctcatt atttatcaca
     1861 aagactaata atcgtagacc aaaatgaatg gtatgaccta tattatccag ctacacttaa
     1921 ttttctcgga atattaccct ccctactttc ctctaccgtt gcgcttatat ccccaccaac
     1981 gttagattta atttaaaaaa tattatttga gacttatcca gttcgcagtg ttaataaata
     2041 gcaaccacac ttaacatctc aatctggggt ataggaagta taaaccacct ttttgctcct
     2101 catccgaagt atcttacctg aaattccctc actcgtttac cgctcaagcc ccaattttaa
     2161 ctgccggtcc agcctaaacc gctctaataa ggttcgattt ggcggtaaaa tctctagcct
     2221 gatagctcga gagatacaaa ctgccccacc gccccgttta aaagttggca gtgttgagca
     2281 gtgttggatt tggggtcgtc agtcaaagag acgactctgt gatggatcga acaggctggg
     2341 agtcagtggc ggcgctcgtt ctggtggcag ctcacgctgc ttggcggcat tcgccttggc
     2401 tgttttctgt ttcagatgct tgagaatctg ctcaatgacc ttcggatctt cgatgctggc
     2461 aatcactttg acgtgaccgc cgcagtgttc gcagacttca atatcaatat tgaagactcg
     2521 cttgaggcgt tgcatccagg tcatggcgcg gtggcgctct gcaggactct tgtcacgcca
     2581 gttagtatcg agaccttccg atttgtcggg cttcttgccc cgcttggcgg gtgttacttg
     2641 aactcggtgt ttgctgttcg gtgcaaagac gccgtggaag cgtgtgaggt tgactcgcgg
     2701 cttaggtacc aacgcagcga gtttggcgat gaagtccagc ggctcgaaga tcacatgggt
     2761 ggtgccattg cggtacggag ttttgagctc gtaacgcacc tgcccattgg cggttaatgc
     2821 cagacgtttt tctgaaaccg ctggccgact aatgtagcga cacaagcgct caagcttatc
     2881 ccgctgatgc gcttcggcca tcacaccggc gtgtagcgag aaaccagcat ggttggctac
     2941 tcgactgctt gagtcggctt tatcctcacg ccctggcaag gtttgcaggg tgaagacttg
     3001 cgcccttgct gggggccgac ggcaatgcga tacgtaaccg aagcaccatg taattgagtc
     3061 agcgtatcgt cttcgccctc ttccagtgtc aaccacgtat tctcggcatc acgctccaaa
     3121 atcccacgct tttccatgca gcgagcgatg cgatggctga gggtgtgagc gagcgtattc
     3181 agctcatcgt aagtgggtgc cttgacacga tggaagcgtt gcttgccata gtcatcttcg
     3241 gcatagacac catcgagaaa cagcatgtgg tagtggacat tgagatttag cgcggagcca
     3301 aagcgttgga taagagtcac tgagccagtt tgtgcagagg ctttggtgta accggctttt
     3361 ttgatcagat gagttgagag tgtacgatag acgatactca agacctggcc catcagctgg
     3421 ggatggcgag ccagcaaaaa gcgtagctgg aaaggaaagc tgagcaccca ctggcgaatg
     3481 ggctccttgg ggaagacttc gtctatcagc agcgccgcac tctcggccat ccggcgggca
     3541 ccgcagctag ggcaaaagcc gcgtcgttta cagctgaagg cgaccagacg ctcgtgatga
     3601 caatcctcgc agcgaacccg catgaaacca tactccagac ggccacattg gaggaggtcg
     3661 ttgaattctt gttggatgta gcgaggcagg tgttgacctt gggcttcgag tgaggctttg
     3721 aaggctgggt agtgctgctc aaccagctgg tagagcagcg tctggtcggg ttggtggcgt
     3781 tcgtaaccgt ttgtttgagt gggcgattga ctcgccgtgg cgttccttgc cagcgacatg
     3841 ggtatcctcc gctgatactg tggttatgta cagtatcagc ggcttgcgtt cagacgtcca
     3901 gtctggccct agacatcgct aaatgcttaa cccgcaatag ccctcacgag ttgttatcag
     3961 ccactaccgg ttgagcgaga aggttttggg ttcagggtgc tattgctcca ccaatcacaa
     4021 tactgaagcc ccaactgtta tcagttgggg ctttttcttg tctgtttgcg gcggttgcgt
     4081 tttatcggta gtcgtcgagc tctgcaccat cccacataag agcttaacgg tgcgatcttc
     4141 aacgccatca cacaaaactt tctttttcac gcacagtcaa cttattggat gttttattaa
     4201 caacccaaaa ggagatattt agcgggcggc cggaaggtga atgctaggca tgatctaacc
     4261 ctcggtctct ggcgtcgcga ctgcgaaatt tcgcgagggt ttccgagaag gtgattgcgc
     4321 ttcgcagatc tccaggcgcg tgggtgcgga cgtagtcagc gccattgccg atcgcgtgaa
     4381 gttccgccgc aaggctcgct ggacccagat cctttacagg aaggccaacg gtggcgccca
     4441 agaaggattt ccgcgacacc gagaccaata gcggaagccc caacgccgac ttcagctttt
     4501 gaaggttcga cagcacgtgc agcgatgttt ccggtgcggg gctcaagaaa aatcccatcc
     4561 ccggatcgag gatgagccgg tcggcagcga ccccgctccg tcgcaaggcg gaaacccgcg
     4621 cctcgaagaa ccgcacaatc tcgtcgagcg cgtcttcggg tcgaaggtga ccggtgcggg
     4681 tggcgatgcc atcccgctgc gctgagtgca taaccaccag cctgcagtcc gcctcagcaa
     4741 tatcgggata gagcgcaggg tcaggaaatc cttggatatc gttcaggtag cccacgccgc
     4801 gcttgagcgc atagcgctgg gtttccggtt ggaagctgtc gattgaaaca cggtgcatct
     4861 gatcggacag ggcgtctaag agcggcgcaa tacgtctgat ctcatcggcc ggcgatacag
     4921 gcctcgcgtc cggatggctg gcggccggtc cgacatccac gacgtctgat ccgactcgca
     4981 gcatttcgat cgccgcggtg acagcgccgg cggggtctag ccgccggctc tcatcgaaga
     5041 aggagtcctc ggtgagattc agaatgccga acaccgtcac catggcgtcg gcctccgcag
     5101 cgacttccac gatggggatc gggcgagcaa aaaggcagca attatgagcc ccatacctac
     5161 aaagccccac gcatcaagct tttgcccatg aagcaaccag gcaatggctg taattatgac
     5221 gacgccgagt cccgaccaga ctgcataagc aacaccgaca gggatggatt tcagaaccag
     5281 agaaagaaaa taaaatgcga tgccataacc gattatgaca acggcggaag gggcaagctt
     5341 agtaaagccc tcgctagatt ttaatgcgga tgttgcgatt acttcgccaa ctattgcgat
     5401 aacaagaaaa agccagcctt tcatgatata tctcccaatt tgtgtagggc ttattatgca
     5461 cg
//
