LOCUS       CP115341                1436 bp    DNA     circular BCT 30-AUG-2023
DEFINITION  Escherichia coli strain CUVET16-242 plasmid pCUVET16-242.8, complete
            sequence.
ACCESSION   CP115341
VERSION     CP115341.1
DBLINK      BioProject: PRJNA914526
            BioSample: SAMN32339849
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Sroithongkham,P., Pinpimai,K., Payungporn,S., Nimsamer,P.,
            Pakdeesanaeha,T., Ponglowhapan,S. and Chanchaithong,P.
  TITLE     Multidrug-resistant extraintestinal pathogenic Escherichia coli
            causing urinary tract, genital tract and bloodstream infections in
            dogs and cats
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Sroithongkham,P. and Chanchaithong,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2022) Department of Veterinary Microbiology,
            Chulalongkorn University, Henri-Dunant, Bangkok 10330, Thailand
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2022
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION and Illumina NextSeq
            550
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/04/2023 15:24:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,228
            CDSs (total)                      :: 5,103
            Genes (coding)                    :: 4,456
            CDSs (with protein)               :: 4,456
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 647
            CDSs (without protein)            :: 647
            Pseudo Genes (ambiguous residues) :: 0 of 647
            Pseudo Genes (frameshifted)       :: 467 of 647
            Pseudo Genes (incomplete)         :: 191 of 647
            Pseudo Genes (internal stop)      :: 57 of 647
            Pseudo Genes (multiple problems)  :: 60 of 647
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CUVET16-242"
                     /isolation_source="Urinary bladder mucosa"
                     /host="dog"
                     /db_xref="taxon:562"
                     /plasmid="pCUVET16-242.8"
                     /geo_loc_name="Thailand: Bangkok"
                     /collection_date="2016-07-03"
                     /collected_by="Pattrarat Chanchaithong"
     gene            34..375
                     /locus_tag="PCS78_26140"
     CDS             34..375
                     /locus_tag="PCS78_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WMO56089.1"
                     /translation="MLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRST
                     IIRWLNYLESKNALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQ
                     RRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          982..1062
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.341"
                     /coverage="87.0"
                     /evalue="2.0600000000000002e-29"
     Typing          complement(1265..1373)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="100.0"
                     /evalue="9.09e-49"
     Typing          complement(607..867)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.603"
                     /coverage="100.0"
                     /evalue="8.62e-98"
ORIGIN
        1 gtatccccaa aaggttggga acgtgttcga gagctgctga aggatagcca aggcatatca
       61 gcactgaagc tgtactcatt tttagcggag catatcgatc ctacgtgtgg cgctgtcgtt
      121 gcggatcagc aattcctagc tgaaaaactt ggagttagca gaagcacaat tattcggtgg
      181 ctcaattact tagaatcaaa aaatgcatta gttagaatcc ccgttgctgg taaggtttgt
      241 gcgtatgccc tcgatccaca tgaagtctgg aagggataca acactacgaa aaaccatgca
      301 gcgtttgtca ctaaaacact ggtcaacaaa gacggtgata ttcagcgccg aatcatggcc
      361 atgttttcaa attgagctag cggcaggcgg acaatcaggg gctacgtgtt aacgttctga
      421 ccatgattgt ctatcctgca ttgctctttt gccgcctcaa aatcctttgc gtgtttttgc
      481 tccccgttct ccagaaaaaa ccgagccgcc acggttccgg cagcgccttg agcgctaccg
      541 gaaaccgtct ggggagaacc ccagaccccc atcgctgcaa caccgacggt ctacgaccac
      601 ctaccaaccg catggctgcc tgttccgtgc aaatgggctt tgcacggggc tggcgcggtt
      661 cctggcgacc tgaaagcccc tggttagggg tatcggcgcg cttgacctcc tgagagcctc
      721 tgtaagcgtt tttcgcgcct tccatgccct ggcgggcatc taaaacccgt ttcgtgcgtc
      781 tgagagcttt tgagcgcgtt tgaggggcta tctggcgact catccccaaa agaacaacac
      841 cgggatcacc gacaacatgc gagaggtggc accgctcgcc gcagccccag cgaccgaacg
      901 ttagtgagcg agtgggcgag gaagcggaag agaaccggaa accacattga gcacgtacgc
      961 actgatgcgg ggtgtcgggg cgaagccctg accagatggc aaacggaata tcgtcgcggg
     1021 tgacggtatt acatttgcac atcctgtccc gatttctgag gcgttttagc ggtgaacgga
     1081 cggcaaaacc atgatggagc tgttgaacgt tgagagcgtg aaatctggca aaaatcacag
     1141 ctggtaaggc ttgcagagga gtaagccagt atacactccg ctatcgctac gtgactgggt
     1201 cagggcttcg ccccgacacc cccaaagggc gttgcgttgc acgcaacacc cttgcctaga
     1261 atagatcttt tacgccgatt tgtaagtggt tgttttttta gtgtgttttt tatttacctg
     1321 tcgcatccag gatgacacaa cgttgtcgca tcctggatgc gacaacacgc aacaaggtgt
     1381 tgcacagatg tgtcatgatg gcttagattt gaaccaacgt tagtgcatgg gatttt
//
